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Wen-Qi Xu,1,2 Jing-Wei Liu,1,2 Xiao-Yu Zhu,1,2 Xiao-Li Zheng,1,2 Kai Chen,1,2 Xiang-Sheng Chen,1,2 Yue-Ping Yin1,2 1STD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, People’s Republic of China; 2STD Reference Laboratory, National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of ChinaCorrespondence: Yue-Ping YinSTD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 12, Jiangwangmiao Street, Nanjing, 210042, People’s Republic of ChinaTel/Fax +86 258 547 8024Email yinyp@ncstdlc.orgPurpose: Neisseria gonorrhoeae, resistant to the first-line treatment option ceftriaxone, is widespread in China from 2016. Nowadays, diverse reagents of disks and strips for rapid gonococcal antimicrobial susceptibility tests used in clinics are culture-based disks diffusion and gradient strips methods. This study aimed to evaluate the accuracy, quality, and availability of almost all disks and strips acquired in the Chinese market and serve as a reference for clinical selection.Methods: We tested the performance of 15 commercial disks and 9 commercial gradient strips acquired in China, compared with traditional agar dilution method. The overall performance was evaluated by the categorical agreement. The reagent accuracy of gradient strips was assessed by the essential agreement.Results: A total of 167 gonococcal isolates were used to evaluate antimicrobial disks from three brands. The overall categorical agreements were 71.7% to 81.8% for ceftriaxone, less than 58% for cefixime, 100% for spectinomycin, over 98% for ciprofloxacin, below 70.5% for penicillin, and 73.3% to 81.8% for tetracycline. A total of 81 isolates were tested for different gradient strips. Categorical agreements were over

Wen-Qi Xu,1,2 Xiao-li Zheng,1,2 Jing-Wei Liu,1,2 Qian Zhou,1,2 Xiao-Yu Zhu,1,2 Jin Zhang,1,2 Yan Han,1,2 Kai Chen,1,2 Shao-Chun Chen,1,2 Xiang-Sheng Chen,1,2 Yue-Ping Yin1,2 1STD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China; 2STD Reference Laboratory, National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of ChinaCorrespondence: Yue-Ping YinSTD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of ChinaTel/Fax +86 258 547 8024Email yinyp@ncstdlc.orgPurpose: This study aimed to determine the minimum inhibitory concentrations (MICs) of ertapenem on Neisseria gonorrhoeae collected from eight Chinese provinces in 2018.Methods: The MICs of ertapenem on 503 Neisseria gonorrhoeae isolates (415 isolates selected randomly and 88 isolates selected with preference) were measured using the agar dilution method. For comparison, the MICs of ceftriaxone and azithromycin were detected.Results: Among 415 randomly selected isolates, the MIC range for ertapenem was from ≤ 0.008 mg/L to 0.5 mg/L. The corresponding MIC50 and MIC90 were 0.06 and 0.125 mg/L, respectively. Twelve of 415 isolates (2.9%) exhibited MIC values ≥ 0.25 mg/L, and only one isolate (0.2%) had a MIC of 0.5 mg/L. By comparing all 503 tested isolates, a correlation of r = 0.487 (P < 0.001) between ertapenem and ceftriaxone MIC was observed, and the correlation between MICs of ertapenem and azithromycin was low (r = − 0.12, P = 0.007). In 24 ceftriaxone-decreased susceptibility isolates, four isolates (16.7%) showed a MIC ≥ 0.25 mg/L for ertapenem. In 85 azithromycin resistant isolates, three isolates (3.5%) showed a MIC ≥ 0.25 mg/L for ertapenem.Conclusion: T

Yan Han,1,2 Yue-Ping Yin,1,2 Jing-Wei Liu,1,2 Kai Chen,1,2 Bang-Yong Zhu,3 Ke Zhou,1,2 Mei-Qin Shi,1,2 Wen-Qi Xu,1,2 Tulip A Jhaveri,4 Xiang-Sheng Chen1,2 1Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China; 2National Center for STD Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of China; 3Institute of Dermatology, Guangxi Autonomous Region, Nanning, People’s Republic of China; 4Division of Medical Microbiology, Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USACorrespondence: Yue-Ping YinInstitute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, 12 Jiangwangmiao Street, Nanjing, 210042, People’s Republic of ChinaTel/Fax +86 258 547 8024Email yinyp@ncstdlc.orgBackground: Previous studies have investigated rectal Mycoplasma genitalium (MG) in men who have sex with men (MSM), while little is known about the prevalence of rectal MG infection in individuals attending sexually transmitted disease (STD) clinics in China. We aimed to estimate the prevalence of rectal MG infection in this population and identified the potential risk factors for rectal MG infection.Methods: A cross-sectional study was conducted among individuals attending STD clinics located in China from June 2018 to August 2020. Univariate and multivariate logistic regression analyses were conducted to explore the association of different risk factors for rectal MG infection.Results: A total of 1,382 patients were included in the final analyses. A total of 30 of 1377 rectal swabs (2.2%) and 77 of 1374 urogenital samples (5.6%) were positive for MG. In Guangxi, 18 of 47 patients (38.3%) infected with urogenital MG and 5 of 19 patients (26.3%) infected with rectal MG received the recommended treatment. Factors found to be significantly associated with rectal MG infection included: male (adjusted odds

Xiao-Li Zheng,1,2 Wen-Qi Xu,1,2 Jing-Wei Liu,1,2 Xiao-Yu Zhu,1,2 Shao-Chun Chen,1,2 Yan Han,1,2 Xiu-Qin Dai,1,2 Isabelle Griffin Goodman,3 Christoph Budjan,4,5 Xiang-Sheng Chen,1,2 Yue-Ping Yin1,2 1Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China; 2National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of China; 3Boston University, Boston, MA, USA; 4Department of Data Sciences, Dana-Farber Cancer Institute, Boston, MA, USA; 5Department of Systems Biology, Harvard Medical School, Boston, MA, USACorrespondence: Yue-Ping YinInstitute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 12, Jiangwangmiao Street, Nanjing 210042, People’s Republic of ChinaTel/Fax +86 258 547 8024Email yinyp@ncstdlc.orgPurpose: The study aimed to evaluate meropenem, fosfomycin, berberine hydrochloride, and doxycycline minimum inhibitory concentrations (MICs) of Neisseria gonorrhoeae collected from eight provinces in China in 2018.Methods: The MICs of 540 Neisseria gonorrhoeae isolates (451 isolates selected randomly and 89 isolates selected with preference) were determined to meropenem, fosfomycin, berberine hydrochloride, and doxycycline using the agar dilution method, and the MICs of ceftriaxone and azithromycin were detected for comparison.Results: Among 451 randomly selected isolates, the MIC90 was 0.06 mg/L for meropenem, 64 mg/L for fosfomycin, 64 mg/L for berberine hydrochloride, and 16 mg/L for doxycycline. All isolates showed the MIC ≤ 0.125 mg/L to meropenem, 13 isolates (2.9%) showed MIC > 64 mg/L to fosfomycin, 8 isolates (1.8%) demonstrated MIC > 64 mg/L to berberine hydrochloride, and 271 isolates (60.1%) demonstrated MIC > 1 mg/L to doxycycline. Comparing all

Shao-Chun Chen,1,2,* Jing-Wei Liu,1,2,* Xing-Zhong Wu,3 Wen-Ling Cao,4 Feng Wang,5 Jin-Mei Huang,3 Yan Han,1,2 Xiao-Yu Zhu,1,2 Bang-Yong Zhu,6 Quan Gan,6 Xiao-Zheng Tang,7 Xing Shen,8 Xiao-Lin Qin,3 Yu-Qi Yu,3 He-Ping Zheng,3 Yue-Ping Yin1,2 1Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China; 2National Center for STD Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of China; 3Clinical Laboratory, Guangdong Provincial Dermatology Hospital, Guangzhou, Guangdong, People’s Republic of China; 4Clinical Laboratory, Guangzhou Dermatology Hospital, Guangzhou, Guangdong, People’s Republic of China; 5Shenzhen Center for Chronic Diseases Control, Shenzhen, Guangdong, People’s Republic of China; 6Clinical Laboratory, Guangxi Provincial Dermatology Hospital, Nanning, Guangxi, People’s Republic of China; 7Clinical Laboratory, Hainan Provincial Dermatology Hospital, Haikou, Hainan, People’s Republic of China; 8Zhuhai Center for Chronic Diseases Control, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yue-Ping YinNational Center for STD Control,Chinese Center for Disease Control and Prevention, 12 Jiangwangmiao Street, Nanjing 210042, People’s Republic of ChinaTel +86-25-85478024Email yinyp@ncstdlc.orgHe-Ping ZhengGuangdong Provincial Dermatology Hospital, 2 Lujing Road, Guangzhou 510091, People’s Republic of ChinaEmail zhhpf@hotmail.comIntroduction: Antimicrobial resistance (AMR) of Neisseria gonorrhoeae (N. gonorrhoeae) becomes a grave public health problem in the world. A strengthened Antimicrobial Resistance Surveillance Program is needed to track the trend of AMR development. However, the lack of a proper antimicrobial susceptibility test (AST) method is a barrier to expand the AMR surveillance in Chin

Jing-Wei Liu,1,2 Wen-Qi Xu,1,2 Xiao-Yu Zhu,1,2 Xiu-Qin Dai,1,2 Shao-Chun Chen,1,2 Yan Han,1,2 Jun Liu,3 Xiang-Sheng Chen,1,2 Yue-Ping Yin1,21Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China; 2National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of China; 3SYNAPSE Center, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USAPurpose: Gentamicin is a promising antimicrobial for the treatment of gonorrhea. The study aimed to evaluate gentamicin minimum inhibitory concentrations (MICs) of Neisseria gonorrhoeae isolates in China.Methods: In this study, the agar dilution method was used to determine the MICs of 470 isolates collected in 2016 to four effective antimicrobials (gentamicin, azithromycin, ceftriaxone, and spectinomycin).Results: Gentamicin MICs ranged from 1 to 8 mg/L. No isolate was resistant to gentamicin. Of seven isolates simultaneously resistant to azithromycin and ceftriaxone, 6 isolates demonstrated MICs of 4 mg/L or less to gentamicin. No cross relationships were found between MICs of gentamicinand susceptibility profiles of azithromycin, ceftriaxone, and spectinomycin.Conclusion: The in vitro results suggest that gentamicin can be a promising treatment option for gonococcal infections in China. Clinical trials to evaluate the therapeutic efficacy of gentamicin are required.Keywords: Neisseria gonorrhoeae, agar dilution method, gentamicin, antimicrobial surveillance

According to the current International Rowing Federation, the distance from the rowing competition is 2000 meters, the match time depending on the different items. The boating operation relies on the paddle hand of the boat to push the boat forward quickly with the work done by the paddle hand. As a result, boating wants to pass the endpoint at less time in a specified distance, and must increase the frequency of rowing per minute on the ship as a power paddle. Increasing the strength of each paddle allows the ship to paddle at the most force of each oar. Each oar hand puts forward the required quality requirements, that is, strength, speed, muscular endurance and rhythm. At present, many scholars have put forward many methods to study sports monitoring at home and abroad, but this sport is rarer in rowing. Therefore, this study will present the intelligent prediction of rowing performance model to predict the performance of the contestants, including: We use four weight methods to defuzzify: (a) dynamic weight forecasting, (b) equal weight, (c) sequential weight, and (d) trends weight. Finally, the forecasting value is optimized by the adaptive adjustment method. Finally, this study uses the public information provided by the International Rowing Alliance website as a training prediction model. The test results are compared with the weight-cutting and forecasting methods.

According to the current International Rowing Federation, the distance from the rowing competition is 2000 meters, the match time depending on the different items. The boating operation relies on the paddle hand of the boat to push the boat forward quickly with the work done by the paddle hand. As a result, boating wants to pass the endpoint at less time in a specified distance, and must increase the frequency of rowing per minute on the ship as a power paddle. Increasing the strength of each paddle allows the ship to paddle at the most force of each oar. Each oar hand puts forward the required quality requirements, that is, strength, speed, muscular endurance and rhythm. At present, many scholars have put forward many methods to study sports monitoring at home and abroad, but this sport is rarer in rowing. Therefore, this study will present the intelligent prediction of rowing performance model to predict the performance of the contestants, including: We use four weight methods to defuzzify: (a) dynamic weight forecasting, (b) equal weight, (c) sequential weight, and (d) trends weight. Finally, the forecasting value is optimized by the adaptive adjustment method. Finally, this study uses the public information provided by the International Rowing Alliance website as a training prediction model. The test results are compared with the weight-cutting and forecasting methods.