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Removal of inorganic phosphate (Pi) from broiler diets could improve sustainability in poultry production. Small-scale studies of total Pi replacement by a novel consensus bacterial 6-phytase variant have been promising but larger scale studies are needed. In this study, effects of 4 diets on growth performance and bone quality were evaluated: PC1, nutritionally adequate and containing Pi without enzyme supplementation; PC2, as PC1 but containing xylanase (2,000 units [XU]/kg) and 75 kcal/kg reduction in ME; IPF1 and IPF2, as PC1 and PC2, respectively, formulated without Pi and 1.5 g/kg reduction in Ca, containing phytase at 3,000, 2,000, and 1,000 phytase units (FTU)/kg during starter (1–10 d), grower (10–22 d), and finisher (22–37 d) phases. A randomized complete block design used 820 Ross 308 mixed-sex birds/pen and 8 pens/diet. The phytase in IPF diets maintained all growth performance parameters, per phase and cumulatively, equivalent to the respective PC. Contrast analysis of pooled PC vs. pooled IPF data showed that phytase in IPF diets reduced or maintained FCR during all phases and cumulatively (−5.0 points during 22–37 d and −3.0 points during 1–37 d; P < 0.05). Tibia ash at d 21 and 36 was not different in birds fed IPF and PC diets, but tibia breaking strength was higher in birds fed IPF diets (+6.6% and +1.3%, respectively; P < 0.05). Overall (0–37 d), total feed costs per kilogram BW gain were reduced (P < 0.05) in the phytase supplemented IPF diets vs. PC diets under commercial broiler production conditions.

1. The effect of increasing the dose level of a novel consensus bacterial 6-phytase variant on apparent ileal digestibility (AID) of phosphorus (P), phytic acid (inositol hexa-phosphate, IP6) and ileal IP6 degradation profile was studied in diets containing varying phytate-P (PP) levels. 2. Ross 308, one-day-old males (n = 1,800) were allocated to cages (20 birds/cage, six cages/treatment) in a completely randomised design employing a 3 × 5 factorial arrangement (three PP levels: 2.45 (low) 2.95 (medium) and 3.45 g/kg (high); five dose levels of phytase (PhyG): 0, 500, 1,000, 2,000 and 4,000 FTU/kg). Phased diets were based on wheat, corn, soybean meal, rapeseed meal and rice bran (d 0 to 10; 2.60 g/kg digestible P, 7.6 g/kg calcium (Ca); d 11 to 21; 2.10 g/kg digestible P, 6.4 g/kg Ca). Ileal digesta was collected on d 21 for determination of P, IP6 and IP-esters content. Data were analysed by factorial ANOVA; means separation was achieved using Tukey’s HSD test. 3. Increasing PP reduced AID of IP6 and sum of IP3-6 (%) (P < 0.05) but absolute P-release (g/kg diet) above NC was increased (P < 0.05) at high vs. low PP. Increasing phytase dose exponentially increased (P < 0.001) AID IP6, sum of IP3-6 (%) and digestible IP3-6-P g/kg diet (P < 0.001). AID P was increased but there was an interaction with PP level (P < 0.001). Ileal accumulation of IP5-3-P was universally low with PhyG at ≥1,000 FTU/kg (<0.06 g/100 g DM). At 2,000 and 4,000 FTU/kg, AID IP6 was 97.2, 92.7, 92.6% and 100, 97.2, 97.1%, respectively, at low, medium and high PP. At 2,000 FTU/kg, phytate-P release estimated as the increase (above NC) in ileal digestible sum of IP3-6-P in the diet was 2.26, 2.59 and 3.10 g/kg in low, medium and high PP, respectively. 4. The data demonstrated that the novel phytase was effective in breaking down phytate to low IP-esters in diets with varied PP content but the optimal dose level for maximising P-release may differ in diets with varying PP content.

Total replacement of dietary inorganic phosphate (Pi) by a novel consensus bacterial 6-phytase variant (PhyG) in phytate-rich diets (>0.3% phytate-P) was investigated in 2 trials using growth performance and bone quality as outcome measures. Both trials utilized a completely randomized design with 5 dietary treatments across 4 phases: starter (0–10 d), grower (10–21 d), finisher 1 (21–35 d), and finisher 2 (35–42 d). Treatments comprised a nutritionally adequate positive control (PC) diet containing monocalcium phosphate and 4 experimental diets (IPF1, IPF2, IPF3, and IPF4), all containing no added Pi and reduced in Ca by 0.2 to 0.3% units vs. PC. IPF1contained PhyG at 1,000 FTU/kg (all phases); IPF2 contained PhyG at 1,000 FTU/kg (all phases) and was additionally reduced in digestible AA, ME, and sodium (−0.2 to −0.4% points, −74 kcal/kg, –0.04% points, respectively, vs. PC); IPF3 contained PhyG at 3,000 FTU/kg in starter, 2,000 FTU/kg in grower, and 1,000 FTU/kg in finisher phases; and IPF4 contained xylanase (2,000 U/kg) and PhyG (2,000 FTU/kg in starter, 1,500 FTU/kg in grower, and 1,000 FTU/kg in finisher phases) and was additionally reduced in ME (–71 kcal/kg vs. PC). Ross 308 broilers were used (trial 1: n = 1,200 mixed sex; 24 birds per pen × 10 replicates; trial 2: n = 1,300 males; 26 birds × 10 replicates). During all phases in both trials, all IPF treatments maintained or improved BW, ADG, ADFI, FCR and BW-corrected FCRc and bone quality parameters vs. PC. vs. PC, treatment IPF3 increased ADG during starter phase (+10.8%) and reduced overall FCRc (−12 points, P < 0.05) in Trial 1, and increased overall ADG (+4.4%), day 35 and day 42 BW (+3.5%, +4.9%), and reduced overall FCRc (−11 points) in Trial 2 (P < 0.05). IPF4 produced equivalent performance to IPF3 (both trials). These are the first data to demonstrate total replacement of Pi by microbial phytase during an entire growth cycle in broiler diets.

Radiation has a limited but relevant role in the adjuvant therapy of gastric cancer (GC) patients. Since Chk1 plays a critical function in cellular response to genotoxic agents, we aimed to analyze the role of Chk1 in GC as a biomarker for radiotherapy resistance. We analyzed Chk1 expression in AGS and MKN45 human GC cell lines by RT-QPCR and WB and in a small cohort of human patient's samples. We demonstrated that Chk1 overexpression specifically increases resistance to radiation in GC cells. Accordingly, abrogation of Chk1 activity with UCN-01 and its expression with shChk1 increased sensitivity to bleomycin and radiation. Furthermore, when we assessed Chk1 expression in human samples, we found a correlation between nuclear Chk1 accumulation and a decrease in progression free survival. Moreover, using a luciferase assay we found that Chk1's expression is controlled by p53 and RB/E2F1 at the transcriptional level. Additionally, we present preliminary data suggesting a posttranscriptional regulation mechanism, involving miR-195 and miR-503, which are inversely correlated with expression of Chk1 in radioresistant cells. In conclusion, Chk1/microRNA axis is involved in resistance to radiation in GC, and suggests Chk1 as a potential tool for optimal stratification of patients susceptible to receive adjuvant radiotherapy after surgery.

We present observations of a new very low mass field binary, discovered during an infrared imaging survey of 250 DENIS L and very late-M dwarfs. DENIS-J055146.0-443412.2 is an M8.5+L0 pair, with a physical separation of over 200 AU. This makes it the widest very low mass binary known in the field, by an order of magnitude. Such a system is fragile, and it would not have survived a close encounter with a third body. Its existence demonstrate that some very low mass stars/brown dwarfs form without ejection from a multiple system, or any other strong dynamical interaction.

We present observations of a new very low mass field binary, discovered during an infrared imaging survey of 250 DENIS L and very late-M dwarfs. DENIS-J055146.0-443412.2 is an M8.5+L0 pair, with a physical separation of over 200 AU. This makes it the widest very low mass binary known in the field, by an order of magnitude. Such a system is fragile, and it would not have survived a close encounter with a third body. Its existence demonstrate that some very low mass stars/brown dwarfs form without ejection from a multiple system, or any other strong dynamical interaction.