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[Background] The European mink (Mustela lutreola, L. 1761) is a critically endangered mustelid, which inhabits several main river drainages in Europe. Here, we assess the genetic variation of existing populations of this species, including new sampling sites and additional molecular markers (newly developed microsatellite loci specific to European mink) as compared to previous studies. Probabilistic analyses were used to examine genetic structure within and between existing populations, and to infer phylogeographic processes and past demography., [Results] According to both mitochondrial and nuclear microsatellite markers, Northeastern (Russia, Estonia and Belarus) and Southeastern (Romania) European populations showed the highest intraspecific diversity. In contrast, Western European (France and Spain) populations were the least polymorphic, featuring a unique mitochondrial DNA haplotype. The high differentiation values detected between Eastern and Western European populations could be the result of genetic drift in the latter due to population isolation and reduction. Genetic differences among populations were further supported by Bayesian clustering and two main groups were confirmed (Eastern vs. Western Europe) along with two contained subgroups at a more local scale (Northeastern vs. Southeastern Europe; France vs. Spain)., [Conclusions] Genetic data and performed analyses support a historical scenario of stable European mink populations, not affected by Quaternary climate oscillations in the Late Pleistocene, and posterior expansion events following river connections in both North- and Southeastern European populations. This suggests an eastern refuge during glacial maxima (as already proposed for boreal and continental species). In contrast, Western Europe was colonised more recently following either natural expansions or putative human introductions. Low levels of genetic diversity observed within each studied population suggest recent bottleneck events and stress the urgent need for conservation measures to counteract the demographic decline experienced by the European mink.

Objective: To study the characteristics of balance performance in a sample of patients with increasing postural instability after acquired brain injury (ABI) and to establish the clinical utility of a new computerized posturographic system (NedSVE/IBV). Methods: This study included 108 patients with ABI divided into five groups from minimal to severe postural impairment. All patients were assessed with the NedSVE/IBV system and with traditional balance measures. Posturographic analyses included the modified clinical test of sensory interaction on balance, the limits of stability and the weight-shifting test. Sensitivity to detect changes and reproducibility were evaluated in 63 patients who were followed-up for 6 months and in 20 patients who were evaluated on two separate occasions during the same week, respectively. Results: The patients showed reduced stability limits, abnormal postural responses and an increased reliance on visual input with differences in intensity directly related to their degree of balance impairment. Posturographic study showed excellent convergent validity, reproducibility and sensitivity to detect changes. Conclusion: The data suggests that, regardless of the intensity of postural instability, there is a common mechanism of sensory processing to maintain balance after ABI. The NedSVE-IBV system is a valid tool to quantify balance after ABI.

Objective: To study the characteristics of balance performance in a sample of patients with increasing postural instability after acquired brain injury (ABI) and to establish the clinical utility of a new computerized posturographic system (NedSVE/IBV). Methods: This study included 108 patients with ABI divided into five groups from minimal to severe postural impairment. All patients were assessed with the NedSVE/IBV system and with traditional balance measures. Posturographic analyses included the modified clinical test of sensory interaction on balance, the limits of stability and the weight-shifting test. Sensitivity to detect changes and reproducibility were evaluated in 63 patients who were followed-up for 6 months and in 20 patients who were evaluated on two separate occasions during the same week, respectively. Results: The patients showed reduced stability limits, abnormal postural responses and an increased reliance on visual input with differences in intensity directly related to their degree of balance impairment. Posturographic study showed excellent convergent validity, reproducibility and sensitivity to detect changes. Conclusion: The data suggests that, regardless of the intensity of postural instability, there is a common mechanism of sensory processing to maintain balance after ABI. The NedSVE-IBV system is a valid tool to quantify balance after ABI.

Orculella bulgarica (Hesse 1915) has been recorded from Bulgaria and western Asia, including Turkey, but has also been reported from Spain by several authors. Most studies on this species have been on subfossil shells. Recent findings of living populations in Turkey and Spain have allowed us to report on the reproductive system morphology and mtDNA sequences of this taxon. Despite the apparent lack of conchological differences between specimens from these two geographical areas, this new information revealed the presence of two species. Review of the literature pertaining to Iberian orculids, led us to conclude that the examined population in Spain species must be assigned to Orculella aragonica (Westerlund 1897), and all previous reports of O. bulgarica from the Iberian Peninsula should be ascribed to the former. This species is redescribed and diagnosed herein, highlighting differences between it and O. bulgarica. Some notes about its conservation status and biogeographic origin are also provided.

Orculella bulgarica (Hesse 1915) has been recorded from Bulgaria and western Asia, including Turkey, but has also been reported from Spain by several authors. Most studies on this species have been on subfossil shells. Recent findings of living populations in Turkey and Spain have allowed us to report on the reproductive system morphology and mtDNA sequences of this taxon. Despite the apparent lack of conchological differences between specimens from these two geographical areas, this new information revealed the presence of two species. Review of the literature pertaining to Iberian orculids, led us to conclude that the examined population in Spain species must be assigned to Orculella aragonica (Westerlund 1897), and all previous reports of O. bulgarica from the Iberian Peninsula should be ascribed to the former. This species is redescribed and diagnosed herein, highlighting differences between it and O. bulgarica. Some notes about its conservation status and biogeographic origin are also provided.

Orculella bulgarica (Hesse 1915) has been recorded from Bulgaria and western Asia, including Turkey, but has also been reported from Spain by several authors. Most studies on this species have been on subfossil shells. Recent findings of living populations in Turkey and Spain have allowed us to report on the reproductive system morphology and mtDNA sequences of this taxon. Despite the apparent lack of conchological differences between specimens from these two geographical areas, this new information revealed the presence of two species. Review of the literature pertaining to Iberian orculids, led us to conclude that the examined population in Spain species must be assigned to Orculella aragonica (Westerlund 1897), and all previous reports of O. bulgarica from the Iberian Peninsula should be ascribed to the former. This species is redescribed and diagnosed herein, highlighting differences between it and O. bulgarica. Some notes about its conservation status and biogeographic origin are also provided.

Orculella bulgarica (Hesse 1915) has been recorded from Bulgaria and western Asia, including Turkey, but has also been reported from Spain by several authors. Most studies on this species have been on subfossil shells. Recent findings of living populations in Turkey and Spain have allowed us to report on the reproductive system morphology and mtDNA sequences of this taxon. Despite the apparent lack of conchological differences between specimens from these two geographical areas, this new information revealed the presence of two species. Review of the literature pertaining to Iberian orculids, led us to conclude that the examined population in Spain species must be assigned to Orculella aragonica (Westerlund 1897), and all previous reports of O. bulgarica from the Iberian Peninsula should be ascribed to the former. This species is redescribed and diagnosed herein, highlighting differences between it and O. bulgarica. Some notes about its conservation status and biogeographic origin are also provided.

Orculella bulgarica (Hesse 1915) has been recorded from Bulgaria and western Asia, including Turkey, but has also been reported from Spain by several authors. Most studies on this species have been on subfossil shells. Recent findings of living populations in Turkey and Spain have allowed us to report on the reproductive system morphology and mtDNA sequences of this taxon. Despite the apparent lack of conchological differences between specimens from these two geographical areas, this new information revealed the presence of two species. Review of the literature pertaining to Iberian orculids, led us to conclude that the examined population in Spain species must be assigned to Orculella aragonica (Westerlund 1897), and all previous reports of O. bulgarica from the Iberian Peninsula should be ascribed to the former. This species is redescribed and diagnosed herein, highlighting differences between it and O. bulgarica. Some notes about its conservation status and biogeographic origin are also provided.

Orculella bulgarica (Hesse 1915) has been recorded from Bulgaria and western Asia, including Turkey, but has also been reported from Spain by several authors. Most studies on this species have been on subfossil shells. Recent findings of living populations in Turkey and Spain have allowed us to report on the reproductive system morphology and mtDNA sequences of this taxon. Despite the apparent lack of conchological differences between specimens from these two geographical areas, this new information revealed the presence of two species. Review of the literature pertaining to Iberian orculids, led us to conclude that the examined population in Spain species must be assigned to Orculella aragonica (Westerlund 1897), and all previous reports of O. bulgarica from the Iberian Peninsula should be ascribed to the former. This species is redescribed and diagnosed herein, highlighting differences between it and O. bulgarica. Some notes about its conservation status and biogeographic origin are also provided.

The European mink (Mustela lutreola L., 1761) is an endangered carnivore species whose populations suffered a severe decline during the last century. The genotyping of eight polymorphic microsatellite loci revealed a relatively low number of alleles per locus (two to eight), as well as low levels of polymorphism (observed and expected heterozygosity values per locus were 0.49 and 0.54, respectively). Cross-specific polymerase chain reaction amplifications were successful in seven closely related mustelid species suggesting that these loci may be useful not only for assessing genetic variability in European mink populations but also for determining potential hybridization events between M. lutreola and other mustelid species.

The European mink (Mustela lutreola L., 1761) is an endangered carnivore species whose populations suffered a severe decline during the last century. The genotyping of eight polymorphic microsatellite loci revealed a relatively low number of alleles per locus (two to eight), as well as low levels of polymorphism (observed and expected heterozygosity values per locus were 0.49 and 0.54, respectively). Cross-specific polymerase chain reaction amplifications were successful in seven closely related mustelid species suggesting that these loci may be useful not only for assessing genetic variability in European mink populations but also for determining potential hybridization events between M. lutreola and other mustelid species.