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7 results on '"Olmo, Roenick P."'

1. Mosquito vector competence for dengue is modulated by insect-specific viruses

Author

Olmo, Roenick P., Todjro, Yaovi M. H., Aguiar, Eric R. G. R., de Almeida, João Paulo P., Ferreira, Flávia V., Armache, Juliana N., de Faria, Isaque J. S., Ferreira, Alvaro G. A., Amadou, Siad C. G., Silva, Ana Teresa S., de Souza, Kátia P. R., Vilela, Ana Paula P., Babarit, Antinea, Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J. M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C. P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A. Z., Drumond, Betânia P., Kroon, Erna G., Recker, Mario, Sedda, Luigi, Marois, Eric, Imler, Jean-Luc, Marques, João T., Olmo, Roenick P., Todjro, Yaovi M. H., Aguiar, Eric R. G. R., de Almeida, João Paulo P., Ferreira, Flávia V., Armache, Juliana N., de Faria, Isaque J. S., Ferreira, Alvaro G. A., Amadou, Siad C. G., Silva, Ana Teresa S., de Souza, Kátia P. R., Vilela, Ana Paula P., Babarit, Antinea, Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J. M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C. P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A. Z., Drumond, Betânia P., Kroon, Erna G., Recker, Mario, Sedda, Luigi, Marois, Eric, Imler, Jean-Luc, and Marques, João T.

Published
2023

2. Mosquito vector competence for dengue is modulated by insect-specific viruses

Author

Olmo, Roenick P., Todjro, Yaovi M. H., Aguiar, Eric R. G. R., de Almeida, João Paulo P., Ferreira, Flávia V., Armache, Juliana N., de Faria, Isaque J. S., Ferreira, Alvaro G. A., Amadou, Siad C. G., Silva, Ana Teresa S., de Souza, Kátia P. R., Vilela, Ana Paula P., Babarit, Antinea, Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J. M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C. P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A. Z., Drumond, Betânia P., Kroon, Erna G., Recker, Mario, Sedda, Luigi, Marois, Eric, Imler, Jean-Luc, Marques, João T., Olmo, Roenick P., Todjro, Yaovi M. H., Aguiar, Eric R. G. R., de Almeida, João Paulo P., Ferreira, Flávia V., Armache, Juliana N., de Faria, Isaque J. S., Ferreira, Alvaro G. A., Amadou, Siad C. G., Silva, Ana Teresa S., de Souza, Kátia P. R., Vilela, Ana Paula P., Babarit, Antinea, Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J. M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C. P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A. Z., Drumond, Betânia P., Kroon, Erna G., Recker, Mario, Sedda, Luigi, Marois, Eric, Imler, Jean-Luc, and Marques, João T.

Published
2023

3. Mosquito vector competence for dengue is modulated by insect-specific viruses

Author

Olmo, Roenick P., Todjro, Yaovi M.H., Aguiar, Eric R.G.R., de Almeida, João Paulo P., Ferreira, Flávia V., Armache, Juliana N., de Faria, Isaque J.S., Ferreira, Alvaro G.A., Amadou, Siad C.G., Silva, Ana Teresa S., de Souza, Kátia P.R., Vilela, Ana Paula P., Babarit, Antinea, Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J.M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C.P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A.Z., Drumond, Betânia P., Kroon, Erna G., Recker, Mario, Sedda, Luigi, Marois, Eric, Imler, Jean Luc, Marques, João T., Olmo, Roenick P., Todjro, Yaovi M.H., Aguiar, Eric R.G.R., de Almeida, João Paulo P., Ferreira, Flávia V., Armache, Juliana N., de Faria, Isaque J.S., Ferreira, Alvaro G.A., Amadou, Siad C.G., Silva, Ana Teresa S., de Souza, Kátia P.R., Vilela, Ana Paula P., Babarit, Antinea, Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J.M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C.P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A.Z., Drumond, Betânia P., Kroon, Erna G., Recker, Mario, Sedda, Luigi, Marois, Eric, Imler, Jean Luc, and Marques, João T.

Abstract

Aedes aegypti and A. albopictus mosquitoes are the main vectors for dengue virus (DENV) and other arboviruses, including Zika virus (ZIKV). Understanding the factors that affect transmission of arboviruses from mosquitoes to humans is a priority because it could inform public health and targeted interventions. Reasoning that interactions among viruses in the vector insect might affect transmission, we analysed the viromes of 815 urban Aedes mosquitoes collected from 12 countries worldwide. Two mosquito-specific viruses, Phasi Charoen-like virus (PCLV) and Humaita Tubiacanga virus (HTV), were the most abundant in A. aegypti worldwide. Spatiotemporal analyses of virus circulation in an endemic urban area revealed a 200% increase in chances of having DENV in wild A. aegypti mosquitoes when both HTV and PCLV were present. Using a mouse model in the laboratory, we showed that the presence of HTV and PCLV increased the ability of mosquitoes to transmit DENV and ZIKV to a vertebrate host. By transcriptomic analysis, we found that in DENV-infected mosquitoes, HTV and PCLV block the downregulation of histone H4, which we identify as an important proviral host factor in vivo.

Published
2023

4. Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus

Author

Lutrat, Célia, Olmo, Roenick P., Baldet, Thierry, Bouyer, Jérémy, Marois, Eric, Lutrat, Célia, Olmo, Roenick P., Baldet, Thierry, Bouyer, Jérémy, and Marois, Eric

Abstract

Aedes albopictus is a major vector of arboviruses. Better understanding of its sex determination is crucial for developing mosquito control tools, especially genetic sexing strains. In Aedes aegypti, Nix is the primary gene responsible for masculinization and Nix-expressing genetic females develop into fertile, albeit flightless, males. In Ae. albopictus, Nix has also been implicated in masculinization but its role remains to be further characterized. In this work, we establish Ae. albopictus transgenic lines ectopically expressing Nix. Several are composed exclusively of genetic females, with transgenic individuals being phenotypic and functional males due to the expression of the Nix transgene. Their reproductive fitness is marginally impaired, while their flight performance is similar to controls. Overall, our results show that Nix is sufficient for full masculinization in Ae. albopictus. Moreover, the transgene construct contains a fluorescence marker allowing efficient automated sex sorting. Consequently, such strains constitute valuable sexing strains for genetic control.

Published
2022

5. Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus

Author

Lutrat, Célia, Olmo, Roenick P., Baldet, Thierry, Bouyer, Jérémy, Marois, Eric, Lutrat, Célia, Olmo, Roenick P., Baldet, Thierry, Bouyer, Jérémy, and Marois, Eric

Abstract

Aedes albopictus is a major vector of arboviruses. Better understanding of its sex determination is crucial for developing mosquito control tools, especially genetic sexing strains. In Aedes aegypti, Nix is the primary gene responsible for masculinization and Nix-expressing genetic females develop into fertile, albeit flightless, males. In Ae. albopictus, Nix has also been implicated in masculinization but its role remains to be further characterized. In this work, we establish Ae. albopictus transgenic lines ectopically expressing Nix. Several are composed exclusively of genetic females, with transgenic individuals being phenotypic and functional males due to the expression of the Nix transgene. Their reproductive fitness is marginally impaired, while their flight performance is similar to controls. Overall, our results show that Nix is sufficient for full masculinization in Ae. albopictus. Moreover, the transgene construct contains a fluorescence marker allowing efficient automated sex sorting. Consequently, such strains constitute valuable sexing strains for genetic control.

Published
2022

6. Insect-specific viruses regulate vector competence in Aedes aegypti mosquitoes via expression of histone H4

Author

Olmo, Roenick P., Todjro, Yaovi M. H., Aguiar, Eric R. G. R., de Almeida, João Paulo P., Armache, Juliana N., de Faria, Isaque J. S., Ferreira, Flávia V., Silva, Ana Teresa S., de Souza, Kátia P. R., Vilela, Ana Paula P., Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J. M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C. P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A. Z., Drumond, Betânia P., Kroon, Erna G., Sedda, Luigi, Marois, Eric, Imler, Jean-Luc, Marques, João T., Olmo, Roenick P., Todjro, Yaovi M. H., Aguiar, Eric R. G. R., de Almeida, João Paulo P., Armache, Juliana N., de Faria, Isaque J. S., Ferreira, Flávia V., Silva, Ana Teresa S., de Souza, Kátia P. R., Vilela, Ana Paula P., Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J. M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C. P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A. Z., Drumond, Betânia P., Kroon, Erna G., Sedda, Luigi, Marois, Eric, Imler, Jean-Luc, and Marques, João T.

Abstract

Aedes aegypti and Aedes albopictus are major mosquito vectors for arthropod-borne viruses (arboviruses) such as dengue (DENV) and Zika (ZIKV) viruses. Mosquitoes also carry insect-specific viruses (ISVs) that may affect the transmission of arboviruses. Here, we analyzed the global virome in urban Aedes mosquitoes and observed that two insect-specific viruses, Phasi Charoen-like virus (PCLV) and Humaita Tubiacanga virus (HTV), were the most prevalent in A. aegypti worldwide except for African cities, where transmission of arboviruses is low. Spatiotemporal analysis revealed that presence of HTV and PCLV led to a 200% increase in the chances of having DENV in wild mosquitoes. In the laboratory, we showed that HTV and PCLV prevented downregulation of histone H4, a previously unrecognized proviral host factor, and rendered mosquitoes more susceptible to DENV and ZIKV. Altogether, our data reveals a molecular basis for the regulation of A. aegypti vector competence by highly prevalent ISVs that may impact how we analyze the risk of arbovirus outbreaks.

Published
2021

7. Insect-specific viruses regulate vector competence in Aedes aegypti mosquitoes via expression of histone H4

Author

Olmo, Roenick P., Todjro, Yaovi M. H., Aguiar, Eric R. G. R., de Almeida, João Paulo P., Armache, Juliana N., de Faria, Isaque J. S., Ferreira, Flávia V., Silva, Ana Teresa S., de Souza, Kátia P. R., Vilela, Ana Paula P., Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J. M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C. P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A. Z., Drumond, Betânia P., Kroon, Erna G., Sedda, Luigi, Marois, Eric, Imler, Jean-Luc, Marques, João T., Olmo, Roenick P., Todjro, Yaovi M. H., Aguiar, Eric R. G. R., de Almeida, João Paulo P., Armache, Juliana N., de Faria, Isaque J. S., Ferreira, Flávia V., Silva, Ana Teresa S., de Souza, Kátia P. R., Vilela, Ana Paula P., Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J. M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C. P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A. Z., Drumond, Betânia P., Kroon, Erna G., Sedda, Luigi, Marois, Eric, Imler, Jean-Luc, and Marques, João T.

Abstract

Aedes aegypti and Aedes albopictus are major mosquito vectors for arthropod-borne viruses (arboviruses) such as dengue (DENV) and Zika (ZIKV) viruses. Mosquitoes also carry insect-specific viruses (ISVs) that may affect the transmission of arboviruses. Here, we analyzed the global virome in urban Aedes mosquitoes and observed that two insect-specific viruses, Phasi Charoen-like virus (PCLV) and Humaita Tubiacanga virus (HTV), were the most prevalent in A. aegypti worldwide except for African cities, where transmission of arboviruses is low. Spatiotemporal analysis revealed that presence of HTV and PCLV led to a 200% increase in the chances of having DENV in wild mosquitoes. In the laboratory, we showed that HTV and PCLV prevented downregulation of histone H4, a previously unrecognized proviral host factor, and rendered mosquitoes more susceptible to DENV and ZIKV. Altogether, our data reveals a molecular basis for the regulation of A. aegypti vector competence by highly prevalent ISVs that may impact how we analyze the risk of arbovirus outbreaks.

Published
2021

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