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Rationale: Aneurysmal subarachnoid hemorrhage (SAH) has high morbidity and mortality. While the primary injury results from the initial bleeding cannot currently be influenced, secondary injury through vasospasm and delayed cerebral ischemia worsens outcome and might be a target for interventions to improve outcome. To date, beside the aneurysm treatment to prevent re-bleeding and the administration of oral nimodipine, there is no therapy available, so novel treatment concepts are needed. Evidence suggests that inflammation contributes to delayed cerebral ischemia and poor outcome in SAH. Some studies suggest a beneficial effect of anti-inflammatory glucocorticoids, but there are no data from randomized controlled trials examining the efficacy of glucocorticoids. Therefore, current guidelines do not recommend the use of glucocorticoids in SAH. Aim: The Fight INflammation to Improve outcome after aneurysmal Subarachnoid HEmorRhage (FINISHER) trial aims to determine whether dexamethasone improves outcome in a clinically relevant endpoint in SAH patients. Methods and design: FINISHER is a multicenter, prospective, randomized, double-blinded, placebo-controlled clinical phase III trial which is testing the outcome and safety of anti-inflammatory treatment with dexamethasone in SAH patients. Sample size estimates: In all, 334 patients will be randomized to either dexamethasone or placebo within 48 h after SAH. The dexamethasone dose is 8 mg tds for days 1-7 and then 8 mg od for days 8-21. Study outcome: The primary outcome is the modified Rankin Scale (mRS) at 6 months, which is dichotomized to favorable (mRS 0-3) versus unfavorable (mRS 4-6). Discussion: The results of this study will provide the first phase III evidence as to whether dexamethasone improves outcome in SAH.

Glioblastoma IV (GBM) is one of the deadliest malignant diseases in adults and is characterized by a high mutation rate and multiple traits to suppress inborn and acquired immunity. We here approached autophagy-related cell death in newly established GBM cell lines derived from individual tumor isolates. Treatment with a small molecule, termed Vacquinol-1 (Vac) exhibited 100% GBM cell death, which was related to mitochondrial dysfunction, calcium-induced endoplasmic reticulum (ER)-stress, and autophagy. The toxicity of Vac was significantly increased by the inhibition of transient receptor potential cation channel, subfamily M, member 7 (TRPM7). TRPM7 is overexpressed in GBM as well as in many other tumors and thus may be a potential target by the natural compound carvacrol. Of note, at higher concentrations, Vac also induced growth inhibition and cell death in non-transformed cell types. However, in the presence of the TRPM7 inhibitor carvacrol, the tumor-selective effect of Vac was very much increased. Results given in the present study are based on long-term video microscopy using IncuCyteZOOM®, calcium measurements, and 3D ultrastructural analysis using the cryofixed material.

Glioblastoma IV (GBM) is one of the deadliest malignant diseases in adults and is characterized by a high mutation rate and multiple traits to suppress inborn and acquired immunity. We here approached autophagy-related cell death in newly established GBM cell lines derived from individual tumor isolates. Treatment with a small molecule, termed Vacquinol-1 (Vac) exhibited 100% GBM cell death, which was related to mitochondrial dysfunction, calcium-induced endoplasmic reticulum (ER)-stress, and autophagy. The toxicity of Vac was significantly increased by the inhibition of transient receptor potential cation channel, subfamily M, member 7 (TRPM7). TRPM7 is overexpressed in GBM as well as in many other tumors and thus may be a potential target by the natural compound carvacrol. Of note, at higher concentrations, Vac also induced growth inhibition and cell death in non-transformed cell types. However, in the presence of the TRPM7 inhibitor carvacrol, the tumor-selective effect of Vac was very much increased. Results given in the present study are based on long-term video microscopy using IncuCyteZOOM®, calcium measurements, and 3D ultrastructural analysis using the cryofixed material.

Čtenář této práce je seznámen se základní myšlenkou iniciativy internetu věcí, je obeznámen se základní meteorologickou terminologií, s meteorologickými veličinami a s jejich vyhodnocováním. Rovněž se dočítá o možné variantě řešení návrhu IoT meteostanice zahrnující hardwarové a softwarové výstupy, návody a popisy vztažené k návrhu a celkové shrnutí problematiky., A reader of this thesis is introduced into basic think about Internet of Things initiation, is informed about elementary meteorologic terminology, about meteorologic units and about their processing. The reader will also read about possible variant of IoT weather station design solution including hardware and software outputs, including guidelines and descriptions related to the design and including final summary of the problematics.

Čtenář této práce je seznámen se základní myšlenkou iniciativy internetu věcí, je obeznámen se základní meteorologickou terminologií, s meteorologickými veličinami a s jejich vyhodnocováním. Rovněž se dočítá o možné variantě řešení návrhu IoT meteostanice zahrnující hardwarové a softwarové výstupy, návody a popisy vztažené k návrhu a celkové shrnutí problematiky., A reader of this thesis is introduced into basic think about Internet of Things initiation, is informed about elementary meteorologic terminology, about meteorologic units and about their processing. The reader will also read about possible variant of IoT weather station design solution including hardware and software outputs, including guidelines and descriptions related to the design and including final summary of the problematics.

Čtenář této práce je seznámen se základní myšlenkou iniciativy internetu věcí, je obeznámen se základní meteorologickou terminologií, s meteorologickými veličinami a s jejich vyhodnocováním. Rovněž se dočítá o možné variantě řešení návrhu IoT meteostanice zahrnující hardwarové a softwarové výstupy, návody a popisy vztažené k návrhu a celkové shrnutí problematiky., A reader of this thesis is introduced into basic think about Internet of Things initiation, is informed about elementary meteorologic terminology, about meteorologic units and about their processing. The reader will also read about possible variant of IoT weather station design solution including hardware and software outputs, including guidelines and descriptions related to the design and including final summary of the problematics.