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6 results on '"Raiteri R"'

1. Real-time multiparameter monitoring of cellular dynamics:an automated microfluidic electrochemical analysis platform

Author

Heiskanen, Arto, Zor, Kinga, Caviglia, Claudia, Vergani, M., Landini, Ettore, Carminati, M., Dimaki, Maria, Svendsen, Winnie Edith, Ferrari, G., Wollenburger, U., Raiteri, R., Dufva, Martin, Sampietro, M., Emnéus, Jenny, Heiskanen, Arto, Zor, Kinga, Caviglia, Claudia, Vergani, M., Landini, Ettore, Carminati, M., Dimaki, Maria, Svendsen, Winnie Edith, Ferrari, G., Wollenburger, U., Raiteri, R., Dufva, Martin, Sampietro, M., and Emnéus, Jenny

Published
2014

2. A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection

Author

Zor, Kinga, Heiskanen, Arto, Caviglia, Claudia, Vergani, M., Landini, E., Shah, Fozia, Carminati, M., Martínez-Serrano, A., Moreno, T. Ramos, Kokaia, M., Benayahu, D., Keresztes, Zs., Papkovsky, D., Wollenberger, U., Svendsen, Winnie Edith, Dimaki, Maria, Ferrari, G., Raiteri, R., Sampietro, M., Dufva, Martin, Emnéus, Jenny, Zor, Kinga, Heiskanen, Arto, Caviglia, Claudia, Vergani, M., Landini, E., Shah, Fozia, Carminati, M., Martínez-Serrano, A., Moreno, T. Ramos, Kokaia, M., Benayahu, D., Keresztes, Zs., Papkovsky, D., Wollenberger, U., Svendsen, Winnie Edith, Dimaki, Maria, Ferrari, G., Raiteri, R., Sampietro, M., Dufva, Martin, and Emnéus, Jenny

Abstract

Downscaling of microfluidic cell culture and detection devices for electrochemical monitoring has mostly focused on miniaturization of the microfluidic chips which are often designed for specific applications and therefore lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility and multifunctionality of the platform, we explored amperometric monitoring of intracellular redox activity in yeast (Saccharomyces cerevisiae) and detection of exocytotically released dopamine from rat pheochromocytoma cells (PC12). Electrochemical impedance spectroscopy was used in both applications for monitoring cell sedimentation and adhesion as well as proliferation in the case of PC12 cells. The influence of flow rate on the signal amplitude in the detection of redox metabolism as well as the effect of mechanical stimulation on dopamine release were demonstrated using the programmable fluid handling capability. The here presented platform is aimed at applications utilizing cell based assays, ranging from e.g. monitoring of drug effects in pharmacological studies, characterization of neural stem cell differentiation, and screening of genetically modified microorganisms to environmental monitoring.

Published
2014

5. Real-time monitoring of cellular dynamics using a microfluidic cell culture system with integrated electrode array and potentiostat

Author

Zor, Kinga, Vergani, M., Heiskanen, Arto, Landini, E., Carminati, M., Coman, V., Vedarethinam, Indumathi, Skafte-Pedersen, Peder, Skolimowski, Maciej, Martinez Serrano, A., Kokaia, M., Ramos Moreno, T., Ghio, A., Svendsen, Winnie Edith, Dimaki, Maria, Keresztes, Zs., Adamovski, M., Wollenberger, U., Sabourin, David, Ferrari, G., Raiteri, R., Sampietro, M., Dufva, Martin, Emnéus, Jenny, Zor, Kinga, Vergani, M., Heiskanen, Arto, Landini, E., Carminati, M., Coman, V., Vedarethinam, Indumathi, Skafte-Pedersen, Peder, Skolimowski, Maciej, Martinez Serrano, A., Kokaia, M., Ramos Moreno, T., Ghio, A., Svendsen, Winnie Edith, Dimaki, Maria, Keresztes, Zs., Adamovski, M., Wollenberger, U., Sabourin, David, Ferrari, G., Raiteri, R., Sampietro, M., Dufva, Martin, and Emnéus, Jenny

Abstract

A versatile microfluidic, multichamber cell culture and analysis system with an integrated electrode array and potentiostat suitable for electrochemical detection and microscopic imaging is presented in this paper. The system, which allows on-line electrode cleaning and modification, was developed for real-time monitoring of cellular dynamics, exemplified in this work by monitoring of redox metabolism inside living yeast cells and dopamine release from PC12 cells.

Published
2011

6. Real-time monitoring of cellular dynamics using a microfluidic cell culture system with integrated electrode array and potentiostat

Author

Zor, Kinga, Vergani, M., Heiskanen, Arto, Landini, E., Carminati, M., Coman, V., Vedarethinam, Indumathi, Skafte-Pedersen, Peder, Skolimowski, Maciej, Martinez Serrano, A., Kokaia, M., Ramos Moreno, T., Ghio, A., Svendsen, Winnie Edith, Dimaki, Maria, Keresztes, Zs., Adamovski, M., Wollenberger, U., Sabourin, David, Ferrari, G., Raiteri, R., Sampietro, M., Dufva, Martin, Emnéus, Jenny, Zor, Kinga, Vergani, M., Heiskanen, Arto, Landini, E., Carminati, M., Coman, V., Vedarethinam, Indumathi, Skafte-Pedersen, Peder, Skolimowski, Maciej, Martinez Serrano, A., Kokaia, M., Ramos Moreno, T., Ghio, A., Svendsen, Winnie Edith, Dimaki, Maria, Keresztes, Zs., Adamovski, M., Wollenberger, U., Sabourin, David, Ferrari, G., Raiteri, R., Sampietro, M., Dufva, Martin, and Emnéus, Jenny

Abstract

A versatile microfluidic, multichamber cell culture and analysis system with an integrated electrode array and potentiostat suitable for electrochemical detection and microscopic imaging is presented in this paper. The system, which allows on-line electrode cleaning and modification, was developed for real-time monitoring of cellular dynamics, exemplified in this work by monitoring of redox metabolism inside living yeast cells and dopamine release from PC12 cells.

Published
2011

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