Search

Your search keyword '"Saiardi, Adolfo"' showing total 12 results
Start Over Author "Saiardi, Adolfo" Database OAIster
12 results on '"Saiardi, Adolfo"'

1. The phytase RipBL1 enables the assignment of a specific inositol phosphate isomer as a structural component of human kidney stones

Author

Liu, Guizhen, Riemer, Esther, Schneider, Robin, Cabuzu, Daniela, Bonny, Olivier, Wagner, Carsten A; https://orcid.org/0000-0002-9874-8898, Qiu, Danye, Saiardi, Adolfo, Strauss, Annett, Lahaye, Thomas, Schaaf, Gabriel, Knoll, Thomas, Jessen, Jan P, Jessen, Henning J; https://orcid.org/0000-0002-1025-9484, Liu, Guizhen, Riemer, Esther, Schneider, Robin, Cabuzu, Daniela, Bonny, Olivier, Wagner, Carsten A; https://orcid.org/0000-0002-9874-8898, Qiu, Danye, Saiardi, Adolfo, Strauss, Annett, Lahaye, Thomas, Schaaf, Gabriel, Knoll, Thomas, Jessen, Jan P, and Jessen, Henning J; https://orcid.org/0000-0002-1025-9484

Abstract

Kidney stones and patient urine contain inositol 1,2,3-trisphosphate as demonstrated by capillary electrophoresis mass spectrometry with an internal heavy isotope reference.

Published
2023

2. Inositol Pyrophosphate-Controlled Kinetochore Architecture and Mitotic Entry in S. pombe

Author

Künzel, Natascha Andrea, Alcázar-Román, Abel R., Saiardi, Adolfo, Bartsch, Simon Maria, Daunaraviciute, Sarune, Fiedler, Dorothea, Fleig, Ursula, Künzel, Natascha Andrea, Alcázar-Román, Abel R., Saiardi, Adolfo, Bartsch, Simon Maria, Daunaraviciute, Sarune, Fiedler, Dorothea, and Fleig, Ursula

Abstract

Inositol pyrophosphates (IPPs) comprise a specific class of signaling molecules that regulate central biological processes in eukaryotes. The conserved Vip1/PPIP5K family controls intracellular IP8 levels, the highest phosphorylated form of IPPs present in yeasts, as it has both inositol kinase and pyrophosphatase activities. Previous studies have shown that the fission yeast S. pombe Vip1/PPIP5K family member Asp1 impacts chromosome transmission fidelity via the modulation of spindle function. We now demonstrate that an IP8 analogue is targeted by endogenous Asp1 and that cellular IP8 is subject to cell cycle control. Mitotic entry requires Asp1 kinase function and IP8 levels are increased at the G2/M transition. In addition, the kinetochore, the conductor of chromosome segregation that is assembled on chromosomes is modulated by IP8. Members of the yeast CCAN kinetochore-subcomplex such as Mal2/CENP-O localize to the kinetochore depending on the intracellular IP8-level: higher than wild-type IP8 levels reduce Mal2 kinetochore targeting, while a reduction in IP8 has the opposite effect. As our perturbations of the inositol polyphosphate and IPP pathways demonstrate that kinetochore architecture depends solely on IP8 and not on other IPPs, we conclude that chromosome transmission fidelity is controlled by IP8 via an interplay between entry into mitosis, kinetochore architecture, and spindle dynamics., Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), Medical Research Council (MRC), Peer Reviewed

Published
2022

3. Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles

Author

Nag, Sudeshna, Rani, Shikha, Mahanty, Sarmistha, Bissig, Christin, Arora, Pooja, Azevedo, Cristina, Saiardi, Adolfo, van der Sluijs, Peter, Delevoye, Cedric, van Niel, Guillaume, Raposo, Graca, Setty, Subba Rao Gangi, Nag, Sudeshna, Rani, Shikha, Mahanty, Sarmistha, Bissig, Christin, Arora, Pooja, Azevedo, Cristina, Saiardi, Adolfo, van der Sluijs, Peter, Delevoye, Cedric, van Niel, Guillaume, Raposo, Graca, and Setty, Subba Rao Gangi

Abstract

Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing melanosomes. However, the mechanism of cargo segregation on SEs is largely unknown. Here, RNAi screening in melanocytes revealed that knockdown of Rab4A results in defective melanosome maturation. Rab4A-depletion increases the number of vacuolar endosomes and disturbs the cargo sorting, which in turn lead to the mislocalization of melanosomal proteins to lysosomes, cell surface and exosomes. Rab4A localizes to the SEs and forms an endosomal complex with the adaptor AP-3, the effector rabenosyn-5 and the motor KIF3, which possibly coordinates cargo segregation on SEs. Consistent with this, inactivation of rabenosyn-5, KIF3A or KIF3B phenocopied the defects observed in Rab4A-knockdown melanocytes. Further, rabenosyn-5 was found to associate with rabaptin-5 or Rabip4/4' (isoforms encoded by Rufy1) and differentially regulate cargo sorting from SEs. Thus, Rab4A acts a key regulator of cargo segregation on SEs.This article has an associated First Person interview with the first author of the paper.

Published
2018

4. Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles

Author

Sub Cellular Protein Chemistry, Cellular Protein Chemistry, Nag, Sudeshna, Rani, Shikha, Mahanty, Sarmistha, Bissig, Christin, Arora, Pooja, Azevedo, Cristina, Saiardi, Adolfo, van der Sluijs, Peter, Delevoye, Cedric, van Niel, Guillaume, Raposo, Graca, Setty, Subba Rao Gangi, Sub Cellular Protein Chemistry, Cellular Protein Chemistry, Nag, Sudeshna, Rani, Shikha, Mahanty, Sarmistha, Bissig, Christin, Arora, Pooja, Azevedo, Cristina, Saiardi, Adolfo, van der Sluijs, Peter, Delevoye, Cedric, van Niel, Guillaume, Raposo, Graca, and Setty, Subba Rao Gangi

Published
2018

5. Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles

Author

Sub Cellular Protein Chemistry, Cellular Protein Chemistry, Nag, Sudeshna, Rani, Shikha, Mahanty, Sarmistha, Bissig, Christin, Arora, Pooja, Azevedo, Cristina, Saiardi, Adolfo, van der Sluijs, Peter, Delevoye, Cedric, van Niel, Guillaume, Raposo, Graca, Setty, Subba Rao Gangi, Sub Cellular Protein Chemistry, Cellular Protein Chemistry, Nag, Sudeshna, Rani, Shikha, Mahanty, Sarmistha, Bissig, Christin, Arora, Pooja, Azevedo, Cristina, Saiardi, Adolfo, van der Sluijs, Peter, Delevoye, Cedric, van Niel, Guillaume, Raposo, Graca, and Setty, Subba Rao Gangi

Published
2018

6. Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles

Author

Sub Cellular Protein Chemistry, Cellular Protein Chemistry, Nag, Sudeshna, Rani, Shikha, Mahanty, Sarmistha, Bissig, Christin, Arora, Pooja, Azevedo, Cristina, Saiardi, Adolfo, van der Sluijs, Peter, Delevoye, Cedric, van Niel, Guillaume, Raposo, Graca, Setty, Subba Rao Gangi, Sub Cellular Protein Chemistry, Cellular Protein Chemistry, Nag, Sudeshna, Rani, Shikha, Mahanty, Sarmistha, Bissig, Christin, Arora, Pooja, Azevedo, Cristina, Saiardi, Adolfo, van der Sluijs, Peter, Delevoye, Cedric, van Niel, Guillaume, Raposo, Graca, and Setty, Subba Rao Gangi

Published
2018

7. VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP8 and Jasmonate-Dependent Defenses in Arabidopsis

Author

Laha, Debabrata, Johnen, Philipp, Azevedo, Cristina, Dynowski, Marek, Weiß, Michael, Capolicchio, Samanta, Mao, Haibin, Iven, Tim, Steenbergen, Merel, Freyer, Marc, Gaugler, Philipp, de Campos, Marília K F, Zheng, Ning, Feussner, Ivo, Jessen, Henning J, van Wees, Saskia C M, Saiardi, Adolfo, Schaaf, Gabriel, Laha, Debabrata, Johnen, Philipp, Azevedo, Cristina, Dynowski, Marek, Weiß, Michael, Capolicchio, Samanta, Mao, Haibin, Iven, Tim, Steenbergen, Merel, Freyer, Marc, Gaugler, Philipp, de Campos, Marília K F, Zheng, Ning, Feussner, Ivo, Jessen, Henning J, van Wees, Saskia C M, Saiardi, Adolfo, and Schaaf, Gabriel

Abstract

Diphosphorylated inositol polyphosphates, also referred to as inositol pyrophosphates, are important signaling molecules that regulate critical cellular activities in many eukaryotic organisms, such as membrane trafficking, telomere maintenance, ribosome biogenesis, and apoptosis. In mammals and fungi, two distinct classes of inositol phosphate kinases mediate biosynthesis of inositol pyrophosphates: Kcs1/IP6K- and Vip1/PPIP5K-like proteins. Here, we report that PPIP5K homologs are widely distributed in plants and that Arabidopsis thaliana VIH1 and VIH2 are functional PPIP5K enzymes. We show a specific induction of inositol pyrophosphate InsP8 by jasmonate and demonstrate that steady state and jasmonate-induced pools of InsP8 in Arabidopsis seedlings depend on VIH2. We identify a role of VIH2 in regulating jasmonate perception and plant defenses against herbivorous insects and necrotrophic fungi. In silico docking experiments and radioligand binding-based reconstitution assays show high-affinity binding of inositol pyrophosphates to the F-box protein COI1-JAZ jasmonate coreceptor complex and suggest that coincidence detection of jasmonate and InsP8 by COI1-JAZ is a critical component in jasmonate-regulated defenses.

Published
2015

8. VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP8 and Jasmonate-Dependent Defenses in Arabidopsis

Author

Plant Microbe Interactions, Sub Plant-Microbe Interactions, Laha, Debabrata, Johnen, Philipp, Azevedo, Cristina, Dynowski, Marek, Weiß, Michael, Capolicchio, Samanta, Mao, Haibin, Iven, Tim, Steenbergen, Merel, Freyer, Marc, Gaugler, Philipp, de Campos, Marília K F, Zheng, Ning, Feussner, Ivo, Jessen, Henning J, van Wees, Saskia C M, Saiardi, Adolfo, Schaaf, Gabriel, Plant Microbe Interactions, Sub Plant-Microbe Interactions, Laha, Debabrata, Johnen, Philipp, Azevedo, Cristina, Dynowski, Marek, Weiß, Michael, Capolicchio, Samanta, Mao, Haibin, Iven, Tim, Steenbergen, Merel, Freyer, Marc, Gaugler, Philipp, de Campos, Marília K F, Zheng, Ning, Feussner, Ivo, Jessen, Henning J, van Wees, Saskia C M, Saiardi, Adolfo, and Schaaf, Gabriel

Published
2015

9. VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP8 and Jasmonate-Dependent Defenses in Arabidopsis

Author

Plant Microbe Interactions, Sub Plant-Microbe Interactions, Laha, Debabrata, Johnen, Philipp, Azevedo, Cristina, Dynowski, Marek, Weiß, Michael, Capolicchio, Samanta, Mao, Haibin, Iven, Tim, Steenbergen, Merel, Freyer, Marc, Gaugler, Philipp, de Campos, Marília K F, Zheng, Ning, Feussner, Ivo, Jessen, Henning J, van Wees, Saskia C M, Saiardi, Adolfo, Schaaf, Gabriel, Plant Microbe Interactions, Sub Plant-Microbe Interactions, Laha, Debabrata, Johnen, Philipp, Azevedo, Cristina, Dynowski, Marek, Weiß, Michael, Capolicchio, Samanta, Mao, Haibin, Iven, Tim, Steenbergen, Merel, Freyer, Marc, Gaugler, Philipp, de Campos, Marília K F, Zheng, Ning, Feussner, Ivo, Jessen, Henning J, van Wees, Saskia C M, Saiardi, Adolfo, and Schaaf, Gabriel

Published
2015

10. VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP8 and Jasmonate-Dependent Defenses in Arabidopsis

Author

Plant Microbe Interactions, Sub Plant-Microbe Interactions, Laha, Debabrata, Johnen, Philipp, Azevedo, Cristina, Dynowski, Marek, Weiß, Michael, Capolicchio, Samanta, Mao, Haibin, Iven, Tim, Steenbergen, Merel, Freyer, Marc, Gaugler, Philipp, de Campos, Marília K F, Zheng, Ning, Feussner, Ivo, Jessen, Henning J, van Wees, Saskia C M, Saiardi, Adolfo, Schaaf, Gabriel, Plant Microbe Interactions, Sub Plant-Microbe Interactions, Laha, Debabrata, Johnen, Philipp, Azevedo, Cristina, Dynowski, Marek, Weiß, Michael, Capolicchio, Samanta, Mao, Haibin, Iven, Tim, Steenbergen, Merel, Freyer, Marc, Gaugler, Philipp, de Campos, Marília K F, Zheng, Ning, Feussner, Ivo, Jessen, Henning J, van Wees, Saskia C M, Saiardi, Adolfo, and Schaaf, Gabriel

Published
2015

11. Are inositol pyrophosphates signalling molecules?

Author

Burton, Adam, Hu, Xiaowen, Saiardi, Adolfo, Burton, Adam, Hu, Xiaowen, and Saiardi, Adolfo

Abstract

The inositol polyphosphate family of small, cytosolic molecules has a prominent place in the field of cell signalling, and inositol pyrophosphates are the most recent addition to this large family. First identified in 1993, they have since been found in all eukaryotic organisms studied. The defining feature of inositol pyrophosphates is the presence of the characteristic ‘high energy’ pyrophosphate group, which immediately attracted interest in them as possible signalling molecules. In addition to their unique ‘high energy’ pyrophosphate bond, their concentration in the cell is tightly regulated with an extremely rapid turnover. This, together with the history of other inositol polyphosphates, makes it likely that they have an important role in intracellular signalling involving some basic cellular processes. This hypothesis is supported by the surprisingly wide range of cellular functions where inositol pyrophosphates seem to be involved. A seminal finding was that inositol pyrophosphates are able to directly phosphorylate pre-phosphorylated proteins, thereby identifying an entirely new post-translational protein modification, namely serine-pyrophosphorylation. Rapid progress has been made in characterising the metabolism of these molecules in the 15 years since their first identification. However, their detailed signalling role in specific cellular processes and in the context of relevant physiological cues has developed more slowly, particularly in mammalian system. We will discuss inositol pyrophosphates from the cell signalling perspective, analysing how their intracellular concentration is modulated, what their possible molecular mechanisms of action are, together with the physiological consequences of this novel form of signalling.

Published
2009

Catalog

Books, media, physical & digital resources
See catalog results