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Nausea is an uncomfortable sensation that accompanies many therapeutics, especially diabetes treatments involving glucagon-like peptide-1 receptor (GLP1R) agonists. Recent studies in mice have revealed that GLP1R-expressing neurons in the area postrema play critical roles in nausea. Here, we characterized a ligand-conjugated saporin that can efficiently ablate GLP1R+ cells from humans, mice, and the Suncus murinus, a small animal model capable of emesis. This new tool provides a strategy to manipulate specific neural pathways in the area postrema in the Suncus murinus and may help elucidate roles of area postrema GLP1R+ neurons in emesis during therapeutics involving GLP1R agonists.

When evolution leads to differences in body size, organs generally scale along. A well-known example of the tight relationship between organ and body size is the scaling of mammalian molar teeth. To investigate how teeth scale during development and evolution, we compared molar development from initiation through final size in the mouse and the rat. Whereas the linear dimensions of the rat molars are twice that of the mouse molars, their shapes are largely the same. Here, we focus on the first lower molars that are considered the most reliable dental proxy for size-related patterns due to their low within-species variability. We found that scaling of the molars starts early, and that the rat molar is patterned equally as fast but in a larger size than the mouse molar. Using transcriptomics, we discovered that a known regulator of body size, insulin-like growth factor 1 (Igf1), is more highly expressed in the rat molars compared to the mouse molars. Ex vivo and in vivo mouse models demonstrated that modulation of the IGF pathway reproduces several aspects of the observed scaling process. Furthermore, analysis of IGF1-treated mouse molars and computational modeling indicate that IGF signaling scales teeth by simultaneously enhancing growth and by inhibiting the cusp-patterning program, thereby providing a relatively simple mechanism for scaling teeth during development and evolution. Finally, comparative data from shrews to elephants suggest that this scaling mechanism regulates the minimum tooth size possible, as well as the patterning potential of large teeth.

The population dynamics of shrews (Soricidae) are not well known even though they form an important part of forest ecosystems and represent suitable bioindicators of ecosystem quality. The aim of this study was to evaluate the population dynamics of shrews in mountain and upland forest clearings in four study areas within the Czech Republic and to reveal how climatic factors influenced fluctuations in their abundance for a decade (2007-2017). In total, we trapped 7,538 individuals of 18 small mammal species. From 760 individuals of seven shrew species, the common shrew (Sorex araneus) was significantly dominated in all study areas. We did not observe any significant, regular multi-annual cycles of the common shrew. However, a cross-correlation in density fluctuation of this species was detected in all mountain areas indicating the influence of environmental factors acting on a larger geographical scale. The autumn abundance of shrews was dependent on the subset of climatic variables, together explaining 56% of the variance in the linear regression model. Except for the length of the snow cover of GREATER-THAN OR EQUAL TO 5 cm, all other significant variables were associated with North Atlantic Oscillation (NAO). Longer duration of snow cover during the winter before trapping, higher average NAO value during months before trapping, and NAO value in September influenced negatively the autumn abundance of shrews, contrary, higher value of NAO in May and October increased the abundance. Our results demonstrate the sensitivity of shrews to winters with a longer period of snow cover and to climatic oscillations associated with the NAO, whose effect is monthly dependent and probably indirectly influencing shrews through their prey., info:eu-repo/semantics/openAccess

The population dynamics of shrews (Soricidae) are not well known even though they form an important part of forest ecosystems and represent suitable bioindicators of ecosystem quality. The aim of this study was to evaluate the population dynamics of shrews in mountain and upland forest clearings in four study areas within the Czech Republic and to reveal how climatic factors influenced fluctuations in their abundance for a decade (2007-2017). In total, we trapped 7,538 individuals of 18 small mammal species. From 760 individuals of seven shrew species, the common shrew (Sorex araneus) was significantly dominated in all study areas. We did not observe any significant, regular multi-annual cycles of the common shrew. However, a cross-correlation in density fluctuation of this species was detected in all mountain areas indicating the influence of environmental factors acting on a larger geographical scale. The autumn abundance of shrews was dependent on the subset of climatic variables, together explaining 56% of the variance in the linear regression model. Except for the length of the snow cover of GREATER-THAN OR EQUAL TO 5 cm, all other significant variables were associated with North Atlantic Oscillation (NAO). Longer duration of snow cover during the winter before trapping, higher average NAO value during months before trapping, and NAO value in September influenced negatively the autumn abundance of shrews, contrary, higher value of NAO in May and October increased the abundance. Our results demonstrate the sensitivity of shrews to winters with a longer period of snow cover and to climatic oscillations associated with the NAO, whose effect is monthly dependent and probably indirectly influencing shrews through their prey., info:eu-repo/semantics/openAccess

The population dynamics of shrews (Soricidae) are not well known even though they form an important part of forest ecosystems and represent suitable bioindicators of ecosystem quality. The aim of this study was to evaluate the population dynamics of shrews in mountain and upland forest clearings in four study areas within the Czech Republic and to reveal how climatic factors influenced fluctuations in their abundance for a decade (2007-2017). In total, we trapped 7,538 individuals of 18 small mammal species. From 760 individuals of seven shrew species, the common shrew (Sorex araneus) was significantly dominated in all study areas. We did not observe any significant, regular multi-annual cycles of the common shrew. However, a cross-correlation in density fluctuation of this species was detected in all mountain areas indicating the influence of environmental factors acting on a larger geographical scale. The autumn abundance of shrews was dependent on the subset of climatic variables, together explaining 56% of the variance in the linear regression model. Except for the length of the snow cover of GREATER-THAN OR EQUAL TO 5 cm, all other significant variables were associated with North Atlantic Oscillation (NAO). Longer duration of snow cover during the winter before trapping, higher average NAO value during months before trapping, and NAO value in September influenced negatively the autumn abundance of shrews, contrary, higher value of NAO in May and October increased the abundance. Our results demonstrate the sensitivity of shrews to winters with a longer period of snow cover and to climatic oscillations associated with the NAO, whose effect is monthly dependent and probably indirectly influencing shrews through their prey., info:eu-repo/semantics/openAccess

The population dynamics of shrews (Soricidae) are not well known even though they form an important part of forest ecosystems and represent suitable bioindicators of ecosystem quality. The aim of this study was to evaluate the population dynamics of shrews in mountain and upland forest clearings in four study areas within the Czech Republic and to reveal how climatic factors influenced fluctuations in their abundance for a decade (2007-2017). In total, we trapped 7,538 individuals of 18 small mammal species. From 760 individuals of seven shrew species, the common shrew (Sorex araneus) was significantly dominated in all study areas. We did not observe any significant, regular multi-annual cycles of the common shrew. However, a cross-correlation in density fluctuation of this species was detected in all mountain areas indicating the influence of environmental factors acting on a larger geographical scale. The autumn abundance of shrews was dependent on the subset of climatic variables, together explaining 56% of the variance in the linear regression model. Except for the length of the snow cover of GREATER-THAN OR EQUAL TO 5 cm, all other significant variables were associated with North Atlantic Oscillation (NAO). Longer duration of snow cover during the winter before trapping, higher average NAO value during months before trapping, and NAO value in September influenced negatively the autumn abundance of shrews, contrary, higher value of NAO in May and October increased the abundance. Our results demonstrate the sensitivity of shrews to winters with a longer period of snow cover and to climatic oscillations associated with the NAO, whose effect is monthly dependent and probably indirectly influencing shrews through their prey.

The dataset contains sampling data of 100 pitfalls that were collected weekly in the Meijendel dune area from March 1953 until March 1960. The dataset contains more than 250 beetle species together with a number of mammal species. The research was done by the Animal Ecology and Plant Ecology group of Leiden University. In particular, drs. G.J. de Bruyn throughout the years has made much effort to empty the pitfalls, identify specimens. In addition, he acted as custodian of the specimen collections and archival records until these could be digitized. Pitfalls were placed in groups of three in replicates of the same set of habitats, running along a gradient from open vegetation dominated by low vegetation (<5cm) and bare sand, through to various stands of woods, groves and shrub dominated vegetations.

Background: Orthohantaviruses and leptospira are emerging zoonotic pathogens of high public health significance. The epidemiology of orthohantavirus infections and leptospirosis is similar and presents related clinical pictures in humans. However, a paucity of data on actual reservoir hosts for orthohantaviruses and leptospira exists. Therefore, this study aimed at determining the occurrence of orthohantaviruses and leptospira in small mammals captured in an endemic region of Sri Lanka. Methods: Rodents and shrews were morphologically and/or genetically identified using morphological keys and DNA barcoding techniques targeting the cytochrome oxidase b subunit gene (Cytb). Lung tissues and sera were subsequently analyzed for the presence of orthohantavirus RNA using qRT-PCR. Sera of rats were tested for IgG antibodies against orthohantaviruses and leptospira. Results: Forty-three (43) small mammals representing: Rattus (R.) rattus (black rat) or R. tanezumi (Asian rat), Suncus murinus (Asian house shrew), R. norvegicus (brown rat) and Mus musculus (house mouse) were investigated. No orthohantavirus RNA was detected from the lung tissue or serum samples of these animals. Elevated levels of IgG antibodies against Puumala orthohantavirus (PUUV) and/or Seoul orthohantavirus (SEOV) antigens were detected in sera of 28 (72%) out of the 39 rats analysed. Interestingly, 36 (92%) of the 39 rats also showed presence of anti leptospira-IgG antibodies in their serum, representing dual infection or dual exposure in 26/39 (66.7%) of examined rats. Conclusions: This project targets important public health questions concerning the occupational risk of orthohantavirus infections and/or leptospirosis in an endemic region of Sri Lanka. Most rats (72%) in our study displayed antibodies reacting to orthohantavirus NP antigens, related to PUUV and/or SEOV. No correlation between the orthohantavirus and leptospira IgG antibody levels were noticed. Finally, a combination of both morphologic

Background: Orthohantaviruses and leptospira are emerging zoonotic pathogens of high public health significance. The epidemiology of orthohantavirus infections and leptospirosis is similar and presents related clinical pictures in humans. However, a paucity of data on actual reservoir hosts for orthohantaviruses and leptospira exists. Therefore, this study aimed at determining the occurrence of orthohantaviruses and leptospira in small mammals captured in an endemic region of Sri Lanka. Methods: Rodents and shrews were morphologically and/or genetically identified using morphological keys and DNA barcoding techniques targeting the cytochrome oxidase b subunit gene (Cytb). Lung tissues and sera were subsequently analyzed for the presence of orthohantavirus RNA using qRT-PCR. Sera of rats were tested for IgG antibodies against orthohantaviruses and leptospira. Results: Forty-three (43) small mammals representing: Rattus (R.) rattus (black rat) or R. tanezumi (Asian rat), Suncus murinus (Asian house shrew), R. norvegicus (brown rat) and Mus musculus (house mouse) were investigated. No orthohantavirus RNA was detected from the lung tissue or serum samples of these animals. Elevated levels of IgG antibodies against Puumala orthohantavirus (PUUV) and/or Seoul orthohantavirus (SEOV) antigens were detected in sera of 28 (72%) out of the 39 rats analysed. Interestingly, 36 (92%) of the 39 rats also showed presence of anti leptospira-IgG antibodies in their serum, representing dual infection or dual exposure in 26/39 (66.7%) of examined rats. Conclusions: This project targets important public health questions concerning the occupational risk of orthohantavirus infections and/or leptospirosis in an endemic region of Sri Lanka. Most rats (72%) in our study displayed antibodies reacting to orthohantavirus NP antigens, related to PUUV and/or SEOV. No correlation between the orthohantavirus and leptospira IgG antibody levels were noticed. Finally, a combination of both morphologic

Background: Orthohantaviruses and leptospira are emerging zoonotic pathogens of high public health significance. The epidemiology of orthohantavirus infections and leptospirosis is similar and presents related clinical pictures in humans. However, a paucity of data on actual reservoir hosts for orthohantaviruses and leptospira exists. Therefore, this study aimed at determining the occurrence of orthohantaviruses and leptospira in small mammals captured in an endemic region of Sri Lanka. Methods: Rodents and shrews were morphologically and/or genetically identified using morphological keys and DNA barcoding techniques targeting the cytochrome oxidase b subunit gene (Cytb). Lung tissues and sera were subsequently analyzed for the presence of orthohantavirus RNA using qRT-PCR. Sera of rats were tested for IgG antibodies against orthohantaviruses and leptospira. Results: Forty-three (43) small mammals representing: Rattus (R.) rattus (black rat) or R. tanezumi (Asian rat), Suncus murinus (Asian house shrew), R. norvegicus (brown rat) and Mus musculus (house mouse) were investigated. No orthohantavirus RNA was detected from the lung tissue or serum samples of these animals. Elevated levels of IgG antibodies against Puumala orthohantavirus (PUUV) and/or Seoul orthohantavirus (SEOV) antigens were detected in sera of 28 (72%) out of the 39 rats analysed. Interestingly, 36 (92%) of the 39 rats also showed presence of anti leptospira-IgG antibodies in their serum, representing dual infection or dual exposure in 26/39 (66.7%) of examined rats. Conclusions: This project targets important public health questions concerning the occupational risk of orthohantavirus infections and/or leptospirosis in an endemic region of Sri Lanka. Most rats (72%) in our study displayed antibodies reacting to orthohantavirus NP antigens, related to PUUV and/or SEOV. No correlation between the orthohantavirus and leptospira IgG antibody levels were noticed. Finally, a combination of both morphologic

Background: Orthohantaviruses and leptospira are emerging zoonotic pathogens of high public health significance. The epidemiology of orthohantavirus infections and leptospirosis is similar and presents related clinical pictures in humans. However, a paucity of data on actual reservoir hosts for orthohantaviruses and leptospira exists. Therefore, this study aimed at determining the occurrence of orthohantaviruses and leptospira in small mammals captured in an endemic region of Sri Lanka. Methods: Rodents and shrews were morphologically and/or genetically identified using morphological keys and DNA barcoding techniques targeting the cytochrome oxidase b subunit gene (Cytb). Lung tissues and sera were subsequently analyzed for the presence of orthohantavirus RNA using qRT-PCR. Sera of rats were tested for IgG antibodies against orthohantaviruses and leptospira. Results: Forty-three (43) small mammals representing: Rattus (R.) rattus (black rat) or R. tanezumi (Asian rat), Suncus murinus (Asian house shrew), R. norvegicus (brown rat) and Mus musculus (house mouse) were investigated. No orthohantavirus RNA was detected from the lung tissue or serum samples of these animals. Elevated levels of IgG antibodies against Puumala orthohantavirus (PUUV) and/or Seoul orthohantavirus (SEOV) antigens were detected in sera of 28 (72%) out of the 39 rats analysed. Interestingly, 36 (92%) of the 39 rats also showed presence of anti leptospira-IgG antibodies in their serum, representing dual infection or dual exposure in 26/39 (66.7%) of examined rats. Conclusions: This project targets important public health questions concerning the occupational risk of orthohantavirus infections and/or leptospirosis in an endemic region of Sri Lanka. Most rats (72%) in our study displayed antibodies reacting to orthohantavirus NP antigens, related to PUUV and/or SEOV. No correlation between the orthohantavirus and leptospira IgG antibody levels were noticed. Finally, a combination of both morphologic

Land-use change is the main driver of biodiversity loss in the Mediterranean basin. New socio-economic conditions produced a rewilding process so that cultural landscapes are being invaded by more natural habitats. We analyze the effects of landscape change on the demography and the spatial distribution of Crocidura russula in six protected areas of the western Mediterranean basin. The study was conducted in the period 2008–2020 on 19 live trapping plots representing the three main natural habitats of the area (scrubland, pinewood, and holm oak woodland). We used a multiscale approach to ensure that the scale of response matched landscape structure (from plot to landscape) using either vegetation profiles (LiDAR) and land use data obtained from years 2007 and 2017. Statistical models (multiple-season single-species occupancy models) showed that C. russula populations were strongly associated to habitat features at the plot level. These models were used to predict occupancy at sampling units for the whole study area (850 km2), showing contrasting trends that shifted at relatively small spatial scales (expansions and retractions of species ranges). Parks showing extreme scrubland encroachment (−8% of area) and afforestation (+6%) significantly reduced habitat suitability for shrews and reductions in occupancy (−5%). Results would indicate faster changes in the spatial distribution of the target species than previously expected on the basis of climate change, driven by fast landscape changes.

Shrews (family Soricidae) are tiny mole-shaped mammals belong to the order Eulipotyphla. The main objective of this study is to screen wild specimens of shrews for specific RNA viruses cause hemorrhagic fevers. Wild specimens of shrews were collected from rural areas in Kingdom of Saudi Arabia. Collection of shrews were carried out from the traps then identified using the classical morphological keys. Specimens were dissected then extraction of single stranded RNA of shrews was performed from internal organs including lungs, livers, kidneys, and stomach using QiagenRNeasy Mini Kit. RT-PCR was utilized for screening of Crimean- Congo hemorrhagic fever virus (CCHFV), Rift Valley Fever Virus (RVFV), and Chikengunya virus (CHIKV), and Sindbis virus (SINV). The results revealed SINV, CHIKV, and CCHFV were all found in the internal viscera of shrews in four different groups. This demonstrated that the viruses were propagating and spreading throughout the tissues of the shrews. Conclusions: Depending to our knowledge results of this study constitute first record in Kingdom Saudi Arabia and significantly highlight some of the neglected wild reservoirs of arboviruses; therefore future studies should focus on evaluating other hosts, including bats., Correction in: Journal of King Saud University Science, Volume 34, Issue 3, Article Number 101862, DOI 10.1016/j.jksus.2022.101862

Shrews (family Soricidae) are tiny mole-shaped mammals belong to the order Eulipotyphla. The main objective of this study is to screen wild specimens of shrews for specific RNA viruses cause hemorrhagic fevers. Wild specimens of shrews were collected from rural areas in Kingdom of Saudi Arabia. Collection of shrews were carried out from the traps then identified using the classical morphological keys. Specimens were dissected then extraction of single stranded RNA of shrews was performed from internal organs including lungs, livers, kidneys, and stomach using QiagenRNeasy Mini Kit. RT-PCR was utilized for screening of Crimean- Congo hemorrhagic fever virus (CCHFV), Rift Valley Fever Virus (RVFV), and Chikengunya virus (CHIKV), and Sindbis virus (SINV). The results revealed SINV, CHIKV, and CCHFV were all found in the internal viscera of shrews in four different groups. This demonstrated that the viruses were propagating and spreading throughout the tissues of the shrews. Conclusions: Depending to our knowledge results of this study constitute first record in Kingdom Saudi Arabia and significantly highlight some of the neglected wild reservoirs of arboviruses; therefore future studies should focus on evaluating other hosts, including bats., Correction in: Journal of King Saud University Science, Volume 34, Issue 3, Article Number 101862, DOI 10.1016/j.jksus.2022.101862

Shrews (family Soricidae) are tiny mole-shaped mammals belong to the order Eulipotyphla. The main objective of this study is to screen wild specimens of shrews for specific RNA viruses cause hemorrhagic fevers. Wild specimens of shrews were collected from rural areas in Kingdom of Saudi Arabia. Collection of shrews were carried out from the traps then identified using the classical morphological keys. Specimens were dissected then extraction of single stranded RNA of shrews was performed from internal organs including lungs, livers, kidneys, and stomach using QiagenRNeasy Mini Kit. RT-PCR was utilized for screening of Crimean- Congo hemorrhagic fever virus (CCHFV), Rift Valley Fever Virus (RVFV), and Chikengunya virus (CHIKV), and Sindbis virus (SINV). The results revealed SINV, CHIKV, and CCHFV were all found in the internal viscera of shrews in four different groups. This demonstrated that the viruses were propagating and spreading throughout the tissues of the shrews. Conclusions: Depending to our knowledge results of this study constitute first record in Kingdom Saudi Arabia and significantly highlight some of the neglected wild reservoirs of arboviruses; therefore future studies should focus on evaluating other hosts, including bats., Correction in: Journal of King Saud University Science, Volume 34, Issue 3, Article Number 101862, DOI 10.1016/j.jksus.2022.101862

A research thesis submitted in partial fulfillment of the requirements for the Degree of Master of Science (Biodiversity Management and Research, Natural habitats have been fragmented by many human activities including farming, hence affecting vegetation structure. Despite being very dry, Namibia has many farms in which game, livestock and crop farming are practiced. Yet such activities affect populations of many other organisms including small mammals such as rodents, shrews and sengis. The present study investigated the effect of habitat type on rodent, shrew and sengi species abundance, richness, diversity and composition in selected open grassland habitat and woody habitats at Karachas Farm, Outjo, Namibia. Sampling at each habitat type was replicated twice. Woody plant species abundance, composition, height and percentage grass cover were determined and compared between the open grassland and woody habitats. A capture-mark-recapture (CMR) technique was employed to obtain data on rodents, shrews and sengis. Each habitat type was sampled over five consecutive nights using Sherman live traps. The result of t-, chi-square and Mann-Whitney tests, respectively, showed a statistically significant difference in the mean abundance of woody plants, plant height, and plant species richness between the open grassland and woody habitats. A Mann-Whitney test showed no statistically significant difference in the percentage grass cover between the open grassland and woody habitats. A Hierarchical Cluster Analysis (HCA) based on a Bray Curtis Similarity Index showed a low (31%) level of similarity in woody plants species composition between the open grassland and woody habitats. A total of 800 trap-nights yielded 145 individuals comprising 6 rodent red veld rat (Aethomys chrysophilus), namaqua rock mouse (Micaelamys namaquensis), bushveld gerbil (Gebilliscus leucogaster), maltimammate mouse (Mastomys natalensis), tree mouse (Thallomys paedulcus), pouched mouse (Saccostomus campestris), sengi (Elephantulus intufi) and shrew (Crocidura hirta) species. t- tests and a Mann- Whitney test, respectively, showed no statistically

Comparative phylogeography has historically been defined as the study of how genetic variation of co-distributed species has been shaped by biogeographical history. This is a mature field of study out of which several techniques have been developed directed at identifying the role ecology and geography in diversification patterns across time. I employ the tools developed in classical comparative phylogeography across multiple taxonomic scales and across regions that historically have been understudied. My first two chapters study the complex history of host-switching, codiversification, and reassortment of hantaviruses in their mammal hosts across North America. By taking a host-centric comparative phylogeographic approach to pathogen divergence, I highlighted complex evolutionary processes in host-pathogen systems and the role of host history in shaping the distribution and diversity of pathogens. My final chapter uses a comparative approach to examine the interplay between ecology and climate in shaping divergence and contact within four East Asian pika species during the Quaternary. I show that small mammals in this vast, poorly studied region responded to Pleistocene climatic cycling on finer geographic scales when compared to the North American fauna that is distributed at similar latitudes.

Introduction. The pathogenic species of the Leptospira genus cause leptospirosis in susceptible humans and animals. Rodents are natural reservoirs of the bacteria; other wild animals are accidental hosts. Shrews have been involved in the transmission cycle of this pathogen in several countries; however, in Mexico, there is no information regarding this. Objective. To describe the infection with Leptospira spp. in shrews captured in Yucatan, Mexico. Material and methods. Two shrew specimens (Cryptotis mayensis) were captured in the municipality of Hunucma. Both were euthanized in order to collect samples from the kidney, spleen, and skeletal muscle for total DNA extraction. Leptospira spp. infection was screened by standard PCR for the amplification of a 16S ribosomal gene fragment (16S rRNA). Likewise, to verify and strengthen the results, other assays were carried out for the amplification of fragments corresponding to the LipL32 and rpoC genes. Results. In the DNA extraction from kidney tissue from one specimen, amplifications of the 16S rRNA and rpoC genes were obtained. Conclusion. This work represents the first report of Leptospira spp. infection in the renal tissue of C. mayensis from Yucatan, Mexico. More studies are needed in the region to determine the participation of these animals in the epidemiological cycle of this bacterial genus., Introducción. Las especies patógenas del género Leptospira ocasionan la leptospirosis en seres humanos y animales susceptibles. Los roedores son los reservorios naturales de estas bacterias; otros animales silvestres son hospederos accidentales. Las musarañas han sido involucradas en el ciclo de transmisión de este patógeno en varios países; sin embargo, en México, no existe información al respecto. Objetivo. Describir la infección con Leptospira spp. en musarañas capturadas en Yucatán, México. Material y métodos. Se capturaron dos ejemplares de musaraña (Cryptotis mayensis) en el municipio de Hunucmá. A ambos se les practicó la eutanasia para recolectar fragmentos de riñón, bazo y músculo esquelético que sirvieron para la extracción de ADN total. La infección con Leptospira spp. se exploró por PCR convencional para la amplificación de un fragmento del gen 16S ribosomal (16S rRNA). Asimismo, para corroborar y robustecer los resultados, se realizaron otras reacciones para la amplificación de fragmentos correspondientes a los genes LipL32 y rpoC. Resultados. En la extracción de ADN correspondiente al tejido renal de un ejemplar se obtuvieron los amplificados de los genes 16S rRNA y rpoC. Conclusión. Este trabajo representa el primer reporte de la infección con Leptospira spp. en tejido renal de C. mayensis de Yucatán, México. Es necesario realizar más estudios en la región para determinar la participación de estos animales en el ciclo epidemiológico del género bacteriano.

Shrews (family Soricidae) are tiny mole-shaped mammals belong to the order Eulipotyphla. The main objective of this study is to screen wild specimens of shrews for specific RNA viruses cause hemorrhagic fevers. Wild specimens of shrews were collected from rural areas in Kingdom of Saudi Arabia. Collection of shrews were carried out from the traps then identified using the classical morphological keys. Specimens were dissected then extraction of single stranded RNA of shrews was performed from internal organs including lungs, livers, kidneys, and stomach using QiagenRNeasy Mini Kit. RT-PCR was utilized for screening of Crimean- Congo hemorrhagic fever virus (CCHFV), Rift Valley Fever Virus (RVFV), and Chikengunya virus (CHIKV), and Sindbis virus (SINV). The results revealed SINV, CHIKV, and CCHFV were all found in the internal viscera of shrews in four different groups. This demonstrated that the viruses were propagating and spreading throughout the tissues of the shrews. Conclusions: Depending to our knowledge results of this study constitute first record in Kingdom Saudi Arabia and significantly highlight some of the neglected wild reservoirs of arboviruses; therefore future studies should focus on evaluating other hosts, including bats., Correction in: Journal of King Saud University Science, Volume 34, Issue 3, Article Number 101862, DOI 10.1016/j.jksus.2022.101862

Comparative phylogeography has historically been defined as the study of how genetic variation of co-distributed species has been shaped by biogeographical history. This is a mature field of study out of which several techniques have been developed directed at identifying the role ecology and geography in diversification patterns across time. I employ the tools developed in classical comparative phylogeography across multiple taxonomic scales and across regions that historically have been understudied. My first two chapters study the complex history of host-switching, codiversification, and reassortment of hantaviruses in their mammal hosts across North America. By taking a host-centric comparative phylogeographic approach to pathogen divergence, I highlighted complex evolutionary processes in host-pathogen systems and the role of host history in shaping the distribution and diversity of pathogens. My final chapter uses a comparative approach to examine the interplay between ecology and climate in shaping divergence and contact within four East Asian pika species during the Quaternary. I show that small mammals in this vast, poorly studied region responded to Pleistocene climatic cycling on finer geographic scales when compared to the North American fauna that is distributed at similar latitudes.

Natural habitats have been fragmented by many human activities including farming, hence affecting vegetation structure. Despite being very dry, Namibia has many farms in which game, livestock and crop farming are practiced. Yet such activities affect populations of many other organisms including small mammals such as rodents, shrews and sengis. The present study investigated the effect of habitat type on rodent, shrew and sengi species abundance, richness, diversity and composition in selected open grassland habitat and woody habitats at Karachas Farm, Outjo, Namibia. Sampling at each habitat type was replicated twice. Woody plant species abundance, composition, height and percentage grass cover were determined and compared between the open grassland and woody habitats. A capture-mark-recapture (CMR) technique was employed to obtain data on rodents, shrews and sengis. Each habitat type was sampled over five consecutive nights using Sherman live traps. The result of t-, chi-square and Mann-Whitney tests, respectively, showed a statistically significant difference in the mean abundance of woody plants, plant height, and plant species richness between the open grassland and woody habitats. A Mann-Whitney test showed no statistically significant difference in the percentage grass cover between the open grassland and woody habitats. A Hierarchical Cluster Analysis (HCA) based on a Bray Curtis Similarity Index showed a low (31%) level of similarity in woody plants species composition between the open grassland and woody habitats. A total of 800 trap-nights yielded 145 individuals comprising 6 rodent red veld rat (Aethomys chrysophilus), namaqua rock mouse (Micaelamys namaquensis), bushveld gerbil (Gebilliscus leucogaster), maltimammate mouse (Mastomys natalensis), tree mouse (Thallomys paedulcus), pouched mouse (Saccostomus campestris), sengi (Elephantulus intufi) and shrew (Crocidura hirta) species. t- tests and a Mann- Whitney test, respectively, showed no statistically

Natural habitats have been fragmented by many human activities including farming, hence affecting vegetation structure. Despite being very dry, Namibia has many farms in which game, livestock and crop farming are practiced. Yet such activities affect populations of many other organisms including small mammals such as rodents, shrews and sengis. The present study investigated the effect of habitat type on rodent, shrew and sengi species abundance, richness, diversity and composition in selected open grassland habitat and woody habitats at Karachas Farm, Outjo, Namibia. Sampling at each habitat type was replicated twice. Woody plant species abundance, composition, height and percentage grass cover were determined and compared between the open grassland and woody habitats. A capture-mark-recapture (CMR) technique was employed to obtain data on rodents, shrews and sengis. Each habitat type was sampled over five consecutive nights using Sherman live traps. The result of t-, chi-square and Mann-Whitney tests, respectively, showed a statistically significant difference in the mean abundance of woody plants, plant height, and plant species richness between the open grassland and woody habitats. A Mann-Whitney test showed no statistically significant difference in the percentage grass cover between the open grassland and woody habitats. A Hierarchical Cluster Analysis (HCA) based on a Bray Curtis Similarity Index showed a low (31%) level of similarity in woody plants species composition between the open grassland and woody habitats. A total of 800 trap-nights yielded 145 individuals comprising 6 rodent red veld rat (Aethomys chrysophilus), namaqua rock mouse (Micaelamys namaquensis), bushveld gerbil (Gebilliscus leucogaster), maltimammate mouse (Mastomys natalensis), tree mouse (Thallomys paedulcus), pouched mouse (Saccostomus campestris), sengi (Elephantulus intufi) and shrew (Crocidura hirta) species. t- tests and a Mann- Whitney test, respectively, showed no statistically

Natural habitats have been fragmented by many human activities including farming, hence affecting vegetation structure. Despite being very dry, Namibia has many farms in which game, livestock and crop farming are practiced. Yet such activities affect populations of many other organisms including small mammals such as rodents, shrews and sengis. The present study investigated the effect of habitat type on rodent, shrew and sengi species abundance, richness, diversity and composition in selected open grassland habitat and woody habitats at Karachas Farm, Outjo, Namibia. Sampling at each habitat type was replicated twice. Woody plant species abundance, composition, height and percentage grass cover were determined and compared between the open grassland and woody habitats. A capture-mark-recapture (CMR) technique was employed to obtain data on rodents, shrews and sengis. Each habitat type was sampled over five consecutive nights using Sherman live traps. The result of t-, chi-square and Mann-Whitney tests, respectively, showed a statistically significant difference in the mean abundance of woody plants, plant height, and plant species richness between the open grassland and woody habitats. A Mann-Whitney test showed no statistically significant difference in the percentage grass cover between the open grassland and woody habitats. A Hierarchical Cluster Analysis (HCA) based on a Bray Curtis Similarity Index showed a low (31%) level of similarity in woody plants species composition between the open grassland and woody habitats. A total of 800 trap-nights yielded 145 individuals comprising 6 rodent red veld rat (Aethomys chrysophilus), namaqua rock mouse (Micaelamys namaquensis), bushveld gerbil (Gebilliscus leucogaster), maltimammate mouse (Mastomys natalensis), tree mouse (Thallomys paedulcus), pouched mouse (Saccostomus campestris), sengi (Elephantulus intufi) and shrew (Crocidura hirta) species. t- tests and a Mann- Whitney test, respectively, showed no statistically

Natural habitats have been fragmented by many human activities including farming, hence affecting vegetation structure. Despite being very dry, Namibia has many farms in which game, livestock and crop farming are practiced. Yet such activities affect populations of many other organisms including small mammals such as rodents, shrews and sengis. The present study investigated the effect of habitat type on rodent, shrew and sengi species abundance, richness, diversity and composition in selected open grassland habitat and woody habitats at Karachas Farm, Outjo, Namibia. Sampling at each habitat type was replicated twice. Woody plant species abundance, composition, height and percentage grass cover were determined and compared between the open grassland and woody habitats. A capture-mark-recapture (CMR) technique was employed to obtain data on rodents, shrews and sengis. Each habitat type was sampled over five consecutive nights using Sherman live traps. The result of t-, chi-square and Mann-Whitney tests, respectively, showed a statistically significant difference in the mean abundance of woody plants, plant height, and plant species richness between the open grassland and woody habitats. A Mann-Whitney test showed no statistically significant difference in the percentage grass cover between the open grassland and woody habitats. A Hierarchical Cluster Analysis (HCA) based on a Bray Curtis Similarity Index showed a low (31%) level of similarity in woody plants species composition between the open grassland and woody habitats. A total of 800 trap-nights yielded 145 individuals comprising 6 rodent red veld rat (Aethomys chrysophilus), namaqua rock mouse (Micaelamys namaquensis), bushveld gerbil (Gebilliscus leucogaster), maltimammate mouse (Mastomys natalensis), tree mouse (Thallomys paedulcus), pouched mouse (Saccostomus campestris), sengi (Elephantulus intufi) and shrew (Crocidura hirta) species. t- tests and a Mann- Whitney test, respectively, showed no statistically

Shrews (family Soricidae) are tiny mole-shaped mammals belong to the order Eulipotyphla. The main objective of this study is to screen wild specimens of shrews for specific RNA viruses cause hemorrhagic fevers. Wild specimens of shrews were collected from rural areas in Kingdom of Saudi Arabia. Collection of shrews were carried out from the traps then identified using the classical morphological keys. Specimens were dissected then extraction of single stranded RNA of shrews was performed from internal organs including lungs, livers, kidneys, and stomach using QiagenRNeasy Mini Kit. RT-PCR was utilized for screening of Crimean- Congo hemorrhagic fever virus (CCHFV), Rift Valley Fever Virus (RVFV), and Chikengunya virus (CHIKV), and Sindbis virus (SINV). The results revealed SINV, CHIKV, and CCHFV were all found in the internal viscera of shrews in four different groups. This demonstrated that the viruses were propagating and spreading throughout the tissues of the shrews. Conclusions: Depending to our knowledge results of this study constitute first record in Kingdom Saudi Arabia and significantly highlight some of the neglected wild reservoirs of arboviruses; therefore future studies should focus on evaluating other hosts, including bats., Correction in: Journal of King Saud University Science, Volume 34, Issue 3, Article Number 101862, DOI 10.1016/j.jksus.2022.101862

Natural habitats have been fragmented by many human activities including farming, hence affecting vegetation structure. Despite being very dry, Namibia has many farms in which game, livestock and crop farming are practiced. Yet such activities affect populations of many other organisms including small mammals such as rodents, shrews and sengis. The present study investigated the effect of habitat type on rodent, shrew and sengi species abundance, richness, diversity and composition in selected open grassland habitat and woody habitats at Karachas Farm, Outjo, Namibia. Sampling at each habitat type was replicated twice. Woody plant species abundance, composition, height and percentage grass cover were determined and compared between the open grassland and woody habitats. A capture-mark-recapture (CMR) technique was employed to obtain data on rodents, shrews and sengis. Each habitat type was sampled over five consecutive nights using Sherman live traps. The result of t-, chi-square and Mann-Whitney tests, respectively, showed a statistically significant difference in the mean abundance of woody plants, plant height, and plant species richness between the open grassland and woody habitats. A Mann-Whitney test showed no statistically significant difference in the percentage grass cover between the open grassland and woody habitats. A Hierarchical Cluster Analysis (HCA) based on a Bray Curtis Similarity Index showed a low (31%) level of similarity in woody plants species composition between the open grassland and woody habitats. A total of 800 trap-nights yielded 145 individuals comprising 6 rodent red veld rat (Aethomys chrysophilus), namaqua rock mouse (Micaelamys namaquensis), bushveld gerbil (Gebilliscus leucogaster), maltimammate mouse (Mastomys natalensis), tree mouse (Thallomys paedulcus), pouched mouse (Saccostomus campestris), sengi (Elephantulus intufi) and shrew (Crocidura hirta) species. t- tests and a Mann- Whitney test, respectively, showed no statistically

In Nepal, rapid urbanization and rural-to-urban migration especially due to internal civil conflict have catalyzed the development of temporary settlements, often along rivers on undeveloped land. This study conducted surveillance for viruses in small mammals and assessed potential risks for virus transmission to people in urban settlements along rivers in Kathmandu, Nepal. We collected samples from 411 small mammals (100 rodents and 311 shrews) at four riverside settlement sites and detected six viruses from four virus families including Thottapalayam virus; a strain of murine coronavirus; two new paramyxoviruses; and two new rhabdoviruses. Additionally, we conducted surveys of 264 residents to characterize animal-human contact. Forty-eight percent of individuals reported contact with wildlife, primarily with rodents and shrews (91%). Our findings confirm that rodents and shrews should be considered a health threat for residents of temporary settlements, and that assessment of disease transmission risk coupled with targeted surveillance for emerging pathogens could lead to improved disease control and health security for urban populations. Additionally, interventions focused on disease prevention should consider the unique urban ecology and social dynamics in temporary settlements, along with the importance of community engagement for identifying solutions that address specific multi-dimensional challenges that life on the urban river margins presents.

In Nepal, rapid urbanization and rural-to-urban migration especially due to internal civil conflict have catalyzed the development of temporary settlements, often along rivers on undeveloped land. This study conducted surveillance for viruses in small mammals and assessed potential risks for virus transmission to people in urban settlements along rivers in Kathmandu, Nepal. We collected samples from 411 small mammals (100 rodents and 311 shrews) at four riverside settlement sites and detected six viruses from four virus families including Thottapalayam virus; a strain of murine coronavirus; two new paramyxoviruses; and two new rhabdoviruses. Additionally, we conducted surveys of 264 residents to characterize animal-human contact. Forty-eight percent of individuals reported contact with wildlife, primarily with rodents and shrews (91%). Our findings confirm that rodents and shrews should be considered a health threat for residents of temporary settlements, and that assessment of disease transmission risk coupled with targeted surveillance for emerging pathogens could lead to improved disease control and health security for urban populations. Additionally, interventions focused on disease prevention should consider the unique urban ecology and social dynamics in temporary settlements, along with the importance of community engagement for identifying solutions that address specific multi-dimensional challenges that life on the urban river margins presents.

Species status statement for the Preble’s shrew (Sorex preblei).

A diversity of Borrelia burgdorferi sensu lato (Johnson, Schmid, Hyde, Steigerwalt & Brenner) (Spirochaetales: Spirochaetaceae) genomospecies, including the Lyme disease agent, Borrelia burgdorferi sensu stricto (s.s.), have been identified in the western United States. However, enzootic transmission of B. burgdorferi s.l. in small mammals and ticks is poorly characterized throughout much of the region. Here we report prevalence of B. burgdorferi s.l. in small mammal and tick communities in the understudied region of southern California. We found B. burgdorferi s.l. in 1.5% of Ixodes species ticks and 3.6% of small mammals. Infection was uncommon (~0.3%) in Ixodes pacificus Cooley and Kohls (Acari: Ixodidae), the primary vector of the Lyme disease agent to humans in western North America, but a diversity of spirochetes-including Borrelia bissettiae, Borrelia californiensis, Borrelia americana, and B. burgdorferi s.s.-were identified circulating in Ixodes species ticks and their small mammal hosts. Infection with B. burgdorferi s.l. is more common in coastal habitats, where a greater diversity of Ixodes species ticks are found feeding on small mammal hosts (four species when compared with only I. pacificus in other sampled habitats). This provides some preliminary evidence that in southern California, wetter coastal areas might be more favorable for enzootic transmission than hotter and drier climates. Infection patterns confirm that human transmission risk of B. burgdorferi s.s. is low in this region. However, given evidence for local maintenance of B. burgdorferi s.l., more studies of enzootic transmission may be warranted, particularly in understudied regions where the tick vector of B. burgdorferi s.s. occurs.

Species status statement for the Preble’s shrew (Sorex preblei).

The genus Crocidura represents a remarkable model for the study of chromosome evolution. This is the case of the lesser white-toothed shrew (Crocidura suaveolens), a representative of the Palearctic group. Although continuously distributed from Siberia to Central Europe, C. suaveolens is a rare, habitat-specialist species in the southwesternmost limit of its distributional range, in the Gulf of Cádiz (Iberian Peninsula). In this area, C. suaveolens is restricted to genetically isolated populations associated to the tidal marches of five rivers (Guadiana, Piedras, Odiel, Tinto and Guadalquivir). This particular distributional range provides a unique opportunity to investigate whether genetic differentiation and habitat specialization was accompanied by chromosomal variation. In this context, the main objective of this study was to determinate the chromosomal characteristics of the habitat-specialist C. suaveolens in Southwestern Iberia, as a way to understand the evolutionary history of this species in the Iberian Peninsula. A total of 41 individuals from six different populations across the Gulf of Cádiz were collected and cytogenetically characterized. We detected four different karyotypes, with diploid numbers (2n) ranging from 2n = 40 to 2n = 43. Two of them (2n = 41 and 2n = 43) were characterized by the presence of B-chromosomes. The analysis of karyotype distribution across lineages and populations revealed an association between mtDNA population divergence and chromosomal differentiation. C. suaveolens populations in the Gulf of Cádiz provide a rare example of true karyotypic polymorphism potentially associated to genetic isolation and habitat specialization in which to investigate the evolutionary significance of chromosomal variation in mammals and their contribution to phenotypic and ecological divergence.

In January 2013, we carried out a mammal inventory in the south lowlands of Guatemala (elevation below 500 m), in the south slope of the Tecuamburro Volcano, in Taxisco, Santa Rosa. The area is immersed in Subtropical Very Wet Forest (Castañeda 2008). We captured a male adult shrew in a pitfall container located within a riparian forest, following the river bank locally known as El Pajal, at 410 msnm (14,119N, -90,505W). The specimen was collected, and prepared as dried skin and skull following the protocols of The Animal Care and Use Committee (Gannon et al. 2007). As a collaboration agreement, it was housed as voucher in the Mammals Collection of El Colegio de la Frontera Sur, San Cristóbal de Las Casas, Mexico, En enero de 2013, realizamos un inventario de mamíferos en las tierras bajas del sur de Guatemala (elevación por debajo de 500 m), en la ladera sur del Volcán Tecuamburro, en Taxisco, Santa Rosa. El área está inmersa en un bosque subtropical muy húmedo (Castañeda 2008). Capturamos una musaraña adulta masculina en un contenedor de trampas ubicado dentro de un bosque ribereño, siguiendo la orilla del río localmente conocida como El Pajal, a 410 msnm (14,119N, -90,505W). El espécimen se recolectó y se preparó como piel y cráneo secos siguiendo los protocolos del Comité de Cuidado y Uso de Animales (Gannon et al. 2007). Como acuerdo de colaboración, se alojó como comprobante en la Colección de Mamíferos de El Colegio de la Frontera Sur, San Cristóbal de Las Casas, México.

We develop a Bayesian method for inferring the species phylogeny under the multispecies coalescent (MSC) model. To improve the mixing properties of the Markov chain Monte Carlo (MCMC) algorithm that traverses the space of species trees, we implement two efficient MCMC proposals: the first is based on the Subtree Pruning and Regrafting (SPR) algorithm and the second is based on a node-slider algorithm. Like the Nearest-Neighbor Interchange (NNI) algorithm we implemented previously, both new algorithms propose changes to the species tree, while simultaneously altering the gene trees at multiple genetic loci to automatically avoid conflicts with the newly proposed species tree. The method integrates over gene trees, naturally taking account of the uncertainty of gene tree topology and branch lengths given the sequence data. A simulation study was performed to examine the statistical properties of the new method. The method was found to show excellent statistical performance, inferring the correct species tree with near certainty when 10 loci were included in the dataset. The prior on species trees has some impact, particularly for small numbers of loci. We analyzed several previously published datasets (both real and simulated) for rattlesnakes and Philippine shrews, in comparison with alternative methods. The results suggest that the Bayesian coalescent-based method is statistically more efficient than heuristic methods based on summary statistics, and that our implementation is computationally more efficient than alternative full-likelihood methods under the MSC. Parameter estimates for the rattlesnake data suggest drastically different evolutionary dynamics between the nuclear and mitochondrial loci, even though they support largely consistent species trees. We discuss the different challenges facing the marginal likelihood calculation and transmodel MCMC as alternative strategies for estimating posterior probabilities for species trees. [Bayes factor; Bayesian infe

As part of a short-term survey for collecting shrews during October 2006, our fieldwork team recorded the coexistence of four species of shrews: 14 individuals of Cryptotis goldmani machetes (10♀ - 4♂), 14 of C. phillipsii (6♀ - 8♂), seven of Sorex salvini oaxacae (7♀), and three of S. veraepacis mutabilis (2♀ - 1♂). All these four taxa are not sister species, even when some of them are congeneric, Como parte de unn trabajo a corto plazo para recolectar musarañas durante octubre de 2006, nuestro equipo de trabajo de campo registró la coexistencia de cuatro especies de musarañas: 14 individuos de machetes Cryptotis goldmani (10♀ - 4♂), 14 de C. phillipsii (6♀ - 8♂), siete de Sorex salvini oaxacae (7♀) y tres de S. veraepacis mutabilis (2♀ - 1♂). Todos estos cuatro taxones no son especies hermanas, incluso cuando algunos de ellos son congenéricos.

Skulls are often used to identify small mammals, and most identification keys to small mammals have been developed on the assumption that whole skulls will be available. However, the skulls of small mammals are seldom found intact in predator pellets or nests, and the bones of several individuals are often scattered and mixed, making counting impossible without the use of a specific cranial part. In addition, only a few keys include all the species found in the eastern provinces of Canada. Mandibles readily resist degradation by the gastric acids of both avian and mammalian predators and are often found intact in food caches of mustelids and in bat hibernacula. We therefore designed an illustrated dichotomous key to small mammals (mean mass <5 kg) of eastern Canada based on diagnostic mandible characters (including the teeth and one dentary bone). We identified and confirmed diagnostic characters to distinguish 55 species from the orders Lagomorpha, Rodentia, Soricomorpha, Carnivora, and Chiroptera. These diagnostic characters are based on a review of the literature and were confirmed by measurements performed on both museum and trapped specimens. In order to facilitate identification, photographic illustrations are provided for each couplet of the key. The ability to identify small mammals using their mandibles will reduce the number of skull components needed and has proven to be a useful tool in the study of the diet of predators. This key may also be helpful in identifying bats in the genera Myotis, Perimyotis, and Eptesicus, which are presently affected by the spread of white-nose syndrome (caused by Pseudogymnoascus destructans) throughout the eastern part of Canada.

BACKGROUND AND PURPOSE: To understand how anandamide transport inhibition impacts the regulation of nausea and vomiting and the receptor level mechanism of action involved. In light of recent characterization of an anandamide transporter, fatty acid amide hydrolase-1-like anandamide transporter, to provide behavioural support for anandamide cellular reuptake as a facilitated transport process. EXPERIMENTAL APPROACH: The systemic administration of the anandamide transport inhibitor ARN272 ([(4-(5-(4-hydroxy-phenyl)-3,4-diaza-bicyclo[4.4.0]deca-1(6),2,4,7,9-pentaen-2-ylamino)-phenyl)-phenylamino-methanone]) was used to evaluate the prevention of LiCl-induced nausea-induced behaviour (conditioned gaping) in rats, and LiCl-induced emesis in shrews (Suncus murinus). The mechanism of how prolonging anandamide availability acts to regulate nausea in rats was explored by the antagonism of cannabinoid 1 (CB1) receptors with the systemic co-administration of SR141716. KEY RESULTS: The systemic administration of ARN272 produced a dose-dependent suppression of nausea-induced conditioned gaping in rats, and produced a dose-dependent reduction of vomiting in shrews. The systemic co-administration of SR141716 with ARN272 (at 3.0 mg·kg(-1)) in rats produced a complete reversal of ARN272-suppressed gaping at 1.0 mg·kg(-1). SR141716 alone did not differ from the vehicle solution. CONCLUSIONS AND IMPLICATIONS: These results suggest that anandamide transport inhibition by the compound ARN272 tonically activates CB1 receptors and as such produces a type of indirect agonism to regulate toxin-induced nausea and vomiting. The results also provide behavioural evidence in support of a facilitated transport mechanism used in the cellular reuptake of anandamide.

Background and purposeTo understand how anandamide transport inhibition impacts the regulation of nausea and vomiting and the receptor level mechanism of action involved. In light of recent characterization of an anandamide transporter, fatty acid amide hydrolase-1-like anandamide transporter, to provide behavioural support for anandamide cellular reuptake as a facilitated transport process.Experimental approachThe systemic administration of the anandamide transport inhibitor ARN272 ([(4-(5-(4-hydroxy-phenyl)-3,4-diaza-bicyclo[4.4.0]deca-1(6),2,4,7,9-pentaen-2-ylamino)-phenyl)-phenylamino-methanone]) was used to evaluate the prevention of LiCl-induced nausea-induced behaviour (conditioned gaping) in rats, and LiCl-induced emesis in shrews (Suncus murinus). The mechanism of how prolonging anandamide availability acts to regulate nausea in rats was explored by the antagonism of cannabinoid 1 (CB1) receptors with the systemic co-administration of SR141716.Key resultsThe systemic administration of ARN272 produced a dose-dependent suppression of nausea-induced conditioned gaping in rats, and produced a dose-dependent reduction of vomiting in shrews. The systemic co-administration of SR141716 with ARN272 (at 3.0 mg·kg(-1)) in rats produced a complete reversal of ARN272-suppressed gaping at 1.0 mg·kg(-1). SR141716 alone did not differ from the vehicle solution.Conclusions and implicationsThese results suggest that anandamide transport inhibition by the compound ARN272 tonically activates CB1 receptors and as such produces a type of indirect agonism to regulate toxin-induced nausea and vomiting. The results also provide behavioural evidence in support of a facilitated transport mechanism used in the cellular reuptake of anandamide.

Background and purposeTo understand how anandamide transport inhibition impacts the regulation of nausea and vomiting and the receptor level mechanism of action involved. In light of recent characterization of an anandamide transporter, fatty acid amide hydrolase-1-like anandamide transporter, to provide behavioural support for anandamide cellular reuptake as a facilitated transport process.Experimental approachThe systemic administration of the anandamide transport inhibitor ARN272 ([(4-(5-(4-hydroxy-phenyl)-3,4-diaza-bicyclo[4.4.0]deca-1(6),2,4,7,9-pentaen-2-ylamino)-phenyl)-phenylamino-methanone]) was used to evaluate the prevention of LiCl-induced nausea-induced behaviour (conditioned gaping) in rats, and LiCl-induced emesis in shrews (Suncus murinus). The mechanism of how prolonging anandamide availability acts to regulate nausea in rats was explored by the antagonism of cannabinoid 1 (CB1) receptors with the systemic co-administration of SR141716.Key resultsThe systemic administration of ARN272 produced a dose-dependent suppression of nausea-induced conditioned gaping in rats, and produced a dose-dependent reduction of vomiting in shrews. The systemic co-administration of SR141716 with ARN272 (at 3.0 mg·kg(-1)) in rats produced a complete reversal of ARN272-suppressed gaping at 1.0 mg·kg(-1). SR141716 alone did not differ from the vehicle solution.Conclusions and implicationsThese results suggest that anandamide transport inhibition by the compound ARN272 tonically activates CB1 receptors and as such produces a type of indirect agonism to regulate toxin-induced nausea and vomiting. The results also provide behavioural evidence in support of a facilitated transport mechanism used in the cellular reuptake of anandamide.

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population whose measurements were significantly different from other populations of Blarina in the state, which resulted in the recognition of another species, B. shermani. Thus, I examined the species as a whole and described how cranial morphology varied geographically. My study examined B. carolinensis in North Carolina and Virginia; compared the three subspecies to determine the amount of difference between them; and also tested for the presence of populations that exhibited noticeably different morphology from the bulk of the species. Specimens were borrowed from 28 museums. The following cranial measurements were recorded for each specimen: occipito-premaxillary length, cranial breadth, maxillary breadth, interorbital breadth, height of mandible, breadth of the condyloid process, and length of molariform tooth row. Principle Components Analysis, Discriminant Function Analysis, and Multivariate Analysis of Variance showed that B. c. peninsulae was often more different than not from B. c. carolinensis and B. c. minima; and there were mixed results for the type and amount of difference between B. c. carolinensis and B. c. minima. These results indicate the existence of two subspecies rather than three. One subspecies, B. c. peninsulae, is retained and is located in peninsular Florida. Blarina caro

Considerable evidence implicates the endocannabinoid system as a neuromodulator of nausea and vomiting. The action of anandamide (AEA) can be prolonged by inhibiting its degradation, through the use of URB597 (URB), a Fatty Acid Amide Hydrolase (FAAH) enzyme inhibitor. Here we present evidence that the FAAH inhibitor, URB, interferes with cisplatin- and nicotine-induced vomiting in the Suncus murinus. In Experiment 1, shrews were injected with URB (0.9 mg/kg) or vehicle 120 min prior to the behavioral testing. They received a second injection of AEA (5 mg/kg) or vehicle 15 min prior to being injected with cisplatin (20 mg/kg) or saline and the number of vomiting episodes were counted for 60 min. In Experiment 2, shrews were injected with vehicle or URB (0.9 mg/kg) 120 min prior to receiving an injection of nicotine (5 mg/kg) or saline and the number of vomiting episodes were counted for 15 min. Experiment 3 evaluated the potential of the CB(1) antagonist, SR141716, to reverse the effect of URB on nicotine-induced vomiting. URB attenuated vomiting produced by cisplatin and nicotine and the combination of URB+AEA suppressed vomiting produced by cisplatin. The effect of URB on nicotine-induced vomiting was reversed by SR141716. These data suggest that the EC system plays a tonic role in the regulation of toxin-induced vomiting.