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Previous research yielded conflicting results on the association between cigarette smoking and risk of SARS-CoV-2 infection. Since the prevalence of smoking is high globally, the study of its impact on COVID-19 pandemic may have considerable implications for public health. This study is the first to investigate the association between the SARS-CoV-2 antibody sero-positivity and biochemically verified smoking status, to refine current estimates on this association. SARS-CoV-2-specific IgG and serum cotinine levels (a well-known marker of tobacco exposure) were assessed in a large sero-epidemiological survey conducted in the town of Troina (Sicily, Italy). A propensity score matching was carried out to reduce the effect of possible factors on SARS-CoV-2 infection risk among study participants. Of the 1785 subjects included in our study, one-third was classified as current smokers, based on serum cotinine levels. The overall proportion of subjects with positive serology for SARS-CoV-2 IgG was 5.4%. The prevalence of SARS-CoV-2 antibody positivity and previous COVID-19 diagnosis were reduced in smokers. This reduced prevalence persisted after adjusting for possible confounders (such as sex, age, previous infection, chronic conditions, and risk group) at regression analyses, and the point estimates based on the PS-matched models resulted consistent with those for the unmatched population. This study found a lower proportion of positive SARS-CoV-2 serology among current smokers, using direct laboratory measures of tobacco exposure and thus avoiding possible bias associated with self-reported smoking status. Results may also serve as a reference for future clinical research on potential pharmaceutical role of nicotine or nicotinic-cholinergic agonists against COVID-19.

Background: After the global spread of SARS-CoV-2, research has highlighted several aspects of the pandemic, focusing on clinical features and risk factors associated with infection and disease severity. However, emerging results on the role of smoking in SARS-CoV-2 infection susceptibility or COVID-19 outcomes are conflicting, and their robustness remains uncertain. Objective: In this context, this study aims at quantifying the proportion of SARS-CoV-2 antibody seroprevalence, studying the changes in antibody levels over time, and analyzing the association between the biochemically verified smoking status and SARS-CoV-2 infection. Methods: The research design involves a 6-month prospective cohort study with a serial sampling of the same individuals. Each participant will be surveyed about their demographics and COVID-19-related information, and blood sampling will be collected upon recruitment and at specified follow-up time points (ie, after 8 and 24 weeks). Blood samples will be screened for the presence of SARS-CoV-2-specific antibodies and serum cotinine, being the latter of the principal metabolite of nicotine, which will be used to assess participants' smoking status. Results: The study is ongoing. It aims to find a higher antibody prevalence in individuals at high risk for viral exposure (ie, health care personnel) and to refine current estimates on the association between smoking status and SARS-CoV-2/COVID-19. Conclusions: The added value of this research is that the current smoking status of the population to be studied will be biochemically verified to avoid the bias associated with self-reported smoking status. As such, the results from this survey may provide an actionable metric to study the role of smoking in SARS-CoV-2 infection and COVID-19 outcomes, and therefore to implement the most appropriate public health measures to control the pandemic. Results may also serve as a reference for future clinical research, and the methodology could be exploite

In India, the coffee root-lesion nematode (Pratylenchus coffeae) is one of the most destructive pests In many coffee growing areas. The present study was attempted to understand the effects of native microbial consortium (i.e. combination of Azospirillum, Azotobacter, Phosphobacteria, Gluconacetobacter and Psettdomonas) along with arbuscutar mycorrhizal (AM) fungi on the control of coffee root-lesion nematode under nursery conditions. The results indicated that application of microbial consortium (5.0 g seedling−1) plus AM fungi (5.0 g seedling−1) on arabica coffee seedlings found to be superior in increasing all the growth parameters viz., leaf area, shoot length, shoot girth, root length, dry weight, leaf chlorophyll, total phenol of leaf and roots with significant reduction in the nematode populations after three months (18.0 number 100 ml−1 of sterile soil and 18.6 number 100 ml−1 of non-sterile soil) and after six months (14,8 number 100 ml−1 of sterile soil and 17.1 number 100 ml−1 non sterile soli) compared to control. Similar to soil nematode population, the microbial consortium plus AM applied treatments recorded significantly lower nematode population in roots after six months of inoculation compared to other treatments.

In India, the coffee root-lesion nematode (Pratylenchus coffeae) is one of the most destructive pests In many coffee growing areas. The present study was attempted to understand the effects of native microbial consortium (i.e. combination of Azospirillum, Azotobacter, Phosphobacteria, Gluconacetobacter and Psettdomonas) along with arbuscutar mycorrhizal (AM) fungi on the control of coffee root-lesion nematode under nursery conditions. The results indicated that application of microbial consortium (5.0 g seedling−1) plus AM fungi (5.0 g seedling−1) on arabica coffee seedlings found to be superior in increasing all the growth parameters viz., leaf area, shoot length, shoot girth, root length, dry weight, leaf chlorophyll, total phenol of leaf and roots with significant reduction in the nematode populations after three months (18.0 number 100 ml−1 of sterile soil and 18.6 number 100 ml−1 of non-sterile soil) and after six months (14,8 number 100 ml−1 of sterile soil and 17.1 number 100 ml−1 non sterile soli) compared to control. Similar to soil nematode population, the microbial consortium plus AM applied treatments recorded significantly lower nematode population in roots after six months of inoculation compared to other treatments.