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Yi He,1,2 Lejia Zhang,1,2 Siyuan Huang,1,2 Yuquan Tang,3 Yiming Li,1,2 Hongyu Li,1,2 Guanhui Chen,4 Xun Chen,1,2 Xiliu Zhang,1,2 Wei Zhao,1,2 Feilong Deng,1,2 Dongsheng Yu1,2 1Hospital of Stomatology, Guanghua School of Stomatology, Institute of Stomatological Research, Sun Yat-sen University, GuangZhou, 510080, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, GuangZhou, 510080, People’s Republic of China; 3Zhujiang Hospital, Southern Medical University, Guangzhou, 510080, People’s Republic of China; 4Department of Stomatology, the Seventh Affiliated Hospital, Sun Yat-sen University, ShenZhen, 518107, People’s Republic of ChinaCorrespondence: Feilong Deng; Dongsheng Yu, Email dengfl@mail.sysu.edu.cn; yudsh@mail.sysu.edu.cnBackground: The ability of nanomaterials to induce osteogenic differentiation is limited, which seriously imped the repair of craniomaxillofacial bone defect. Magnetic graphene oxide (MGO) nanocomposites with the excellent physicochemical properties have great potential in bone tissue engineering. In this study, we aim to explore the craniomaxillofacial bone defect repairment effect of MGO nanocomposites and its underlying mechanism.Methods: The biocompatibility of MGO nanocomposites was verified by CCK8, live/dead staining and cytoskeleton staining. The function of MGO nanocomposites induced osteogenic differentiation of BMSCs was investigated by ALP activity detection, mineralized nodules staining, detection of osteogenic genes and proteins, and immune-histochemical staining. BMSCs with or without MGO osteogenic differentiation induction were collected and subjected to high-throughput circular ribonucleic acids (circRNAs) sequencing, and then crucial circRNA circAars was screened and identified. Bioinformatics analysis, Dual-luciferase reporter assay, RNA binding protein immunoprecipitation (RIP), fluorescence in situ hybridization (FISH) and osteogenic-related examinations were used

Lejia Zhang,* Kuangwu Pan,* Siyuan Huang, Xiliu Zhang, Xinyu Zhu, Yi He, Xun Chen, Yuquan Tang, Lingyu Yuan, Dongsheng Yu Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dongsheng Yu, Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Guangzhou, People’s Republic of China, Email yudsh@mail.sysu.edu.cnPurpose: Nanomaterial-based photodynamic therapy (PDT) has been commonly used for the treatment of cancerous tumors. Despite significant achievements made in this field, the intrinsic impact of nanomaterials-based PDT on the mechanical properties of oral squamous cell carcinoma (OSCC) cells is not entirely understood. Here, we used atomic force microscopy (AFM) to measure the stiffness of OSCC cells subjected to PDT in co-culture systems to evaluate the T cell-mediated cancer cell-killing effects.Methods: In this study, AFM was used to assess the stiffness of PDT-subjected cells. The phototoxicity of graphdiyne oxide (GDYO) was assessed using confocal laser scanning microscopy (CLSM), measurements of membrane cholesterol levels, and assessments of the F-actin cytoskeleton. A co-culture system was used to evaluate the effects of CD8+ T cells (cytotoxic T lymphocytes), demonstrating how PDT modulates the mechanical properties of cancer cells and activates T cell responses. The antitumor immunotherapeutic effect of GDYO was further evaluated in a murine xenograft model.Results: GDYO increased the mechanical stiffness of tumor cells and augmented T-cell cytotoxicity and inflammatory cytokine secretion (IFN-γ and TNF-α) under laser in vitro. Furthermore, GDYO-based PDT exerted inhibitory effects on OSCC models and elicited antitumor immune responses via specific cytotoxic T

Changes in the stiffness of chondrocyte extracellular matrix (ECM) are involved in the pathological progression of osteoarthritis (OA). However, the downstream responses of cartilage ECM stiffness are still unclear. YAP (Yes-associated protein) has been extensively studied as a mechanotransducer, we thus hypothesized that by targeting the downstream molecule activity of ECM stiffness could maintain chondrocyte phenotype and prevent cartilage degeneration in OA. Here, we showed that human cartilage matrix stiffened during pathological progression of OA, and the chondrocyte YAP activity was associated with ECM stiffness. We then mimicked the physiological and pathological stiffness of human cartilage by using PDMS-based substrates, and found that YAP was activated in chondrocytes seeded on stiff substrate, gradually losing their phenotype. In addition, it was observed that YAP was also significantly activated in mice OA development, and conditional knockout (cKO) of YAP in mice preserved collagen II expression and protected cartilage from degeneration in the OA model. Furthermore, intra-articular injection of YAP-selective inhibitor, Verteporfin, significantly maintained cartilage homeostasis in mice OA model. This study indicates that the application of mechanotransducer-targeted drugs could be a potential therapeutic approach for cartilage repair in OA.

Xiliu Zhang,1 Hongyu Li,1,* Chen Yi,1,* Guosheng Chen,2 Ye Li,1 Ying Zhou,1 Guanhui Chen,1 Yiming Li,1 Yi He,1 Dongsheng Yu1 1Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, People’s Republic of China; 2MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dongsheng YuGuanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, People’s Republic of ChinaEmail yudsh@mail.sysu.edu.cnIntroduction: With the innovation of photosensitizers, photodynamic therapy is now widely used in antitumor detection and treatment. Graphene quantum dots (GQDs) are proposed as a promising alternative photosensitizer due to their high biocompatibility, specific photoactivity, and strong tumor concentration. However, the changes in host immunity triggered by GQDs have only rarely been reported.Methods: In this work, GQDs as photosensitizers were conjugated to polyethylene glycol (PEG) to enhance solubility and blood circulation. The phototoxicity of the resulting GQD-PEG nanomaterials was then detected in vitro and in vivo. The antitumor immunity triggered by GQD-PEG under irradiation was further evaluated in an oral squamous cell carcinoma animal model.Results: The obtained GQD-PEG nanomaterials exhibited low cytotoxicity, good solution stability, and excellent endocytosis. Both in vitro and in vivo, all demonstrated strong ablation for oral squamous cell carcinoma under irradiation. Meanwhile, host-immunity-related CD8+ T cells (cytotoxic T lymphocytes) and proinflammatory cytokines, including IFN-γ and TNF-α, were significantly increased after photo-activated antitumor activity.Conclusion: The

Xiliu Zhang,1 Hongyu Li,1,* Chen Yi,1,* Guosheng Chen,2 Ye Li,1 Ying Zhou,1 Guanhui Chen,1 Yiming Li,1 Yi He,1 Dongsheng Yu1 1Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, People’s Republic of China; 2MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dongsheng YuGuanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, People’s Republic of ChinaEmail yudsh@mail.sysu.edu.cnIntroduction: With the innovation of photosensitizers, photodynamic therapy is now widely used in antitumor detection and treatment. Graphene quantum dots (GQDs) are proposed as a promising alternative photosensitizer due to their high biocompatibility, specific photoactivity, and strong tumor concentration. However, the changes in host immunity triggered by GQDs have only rarely been reported.Methods: In this work, GQDs as photosensitizers were conjugated to polyethylene glycol (PEG) to enhance solubility and blood circulation. The phototoxicity of the resulting GQD-PEG nanomaterials was then detected in vitro and in vivo. The antitumor immunity triggered by GQD-PEG under irradiation was further evaluated in an oral squamous cell carcinoma animal model.Results: The obtained GQD-PEG nanomaterials exhibited low cytotoxicity, good solution stability, and excellent endocytosis. Both in vitro and in vivo, all demonstrated strong ablation for oral squamous cell carcinoma under irradiation. Meanwhile, host-immunity-related CD8+ T cells (cytotoxic T lymphocytes) and proinflammatory cytokines, including IFN-γ and TNF-α, were significantly increased after photo-activated antitumor activity.Conclusion: The

The fifth generation (5G) wireless access technology, known as New Radio (NR), features flexibility to support a variety of usage scenarios. One of the basic concepts in 5G NR is bandwidth part (BWP), which is, at a high level, a set of contiguous resource blocks configured inside a channel bandwidth. BWP spans across many 5G NR specifications developed by the 3rd Generation Partnership Project. Understanding how BWP operates is vital to understanding 5G NR. This article provides an overview of the essentials of BWP in the NR technical specifications. We describe fundamental BWP concepts, BWP configuration methods, and BWP switch mechanisms. We also discuss user equipment capabilities in terms of BWP support and share our thoughts on use cases of BWP for NR deployments., Comment: 7 pages, 3 figures, 2 tables

Using the DeNitrification-DeComposition (DNDC, version 9.5) model, we investigated the soil organic carbon (SOC) changes from 1980 to 2009 in Eastern China's upland-crop fields in northern Jiangsu Province. A currently most detailed high-resolution soil database, containing 17,024 polygons at a scale of 1:50,000, derived from 983 unique upland soil profiles, was used. A coarser county-level soil database was also used for a pair-wise simulation for comparison. We found that SOC changes modeled with the county-level soil database differ significantly from those with high-resolution soil data, with the deviation ranging from -64% to 8.0% in different counties. This implies that coarse soil data may lead to large biases in SOC simulation. With the high-resolution database, the model estimates a SOC increase of 37.89 TgC in the top soils (0-50 cm) over the study area of 3.93 Mha for the past three decades, with an average rate of 322 kg C ha(-1) year(-1). The SOC accumulation in the study region accounts for 10.2% of annual national carbon sequestration of upland soils, compared with the fraction of 3.7% in the total upland area of China. This underscores its significance to national climate mitigation. The annual SOC change varied between 61 to 519 kg C ha(-1) year mainly driven by the variations in N-fertilizer and manure applications. This study highlights the significance of high-resolution soil databases in quantifying SOC changes. Our high-resolution estimates of SOC will support farming and carbon management in this region. (C) 2016 Elsevier B.V. All rights reserved.

Agro-ecosystem models have been widely used to quantify soil organic carbon (SOC) dynamics based on digital soil maps. However, most of the studies use soil data of single or limited choices of map scales, thus the influence of map scales on SOC dynamics has rarely been quantified. In this study, six digital paddy soils databases of the Tai -Lake region in China at scales of 1:50,000 (P005),1:200,000 (P02),1:500,000 (P05), 1:1,000,000 (P1), 1:4,000,000 (P4), and 1:14,000,000 (P14) were used to drive the DNDC (DeNitrification & DeComposition) model to quantify SOC dynamics for the period of 2001-2019. Model simulations show that the total SOC changes from 2001 to 2019 in the top layer (0-30 cm) of paddy soils using P005, P02, P05, P1, P4, and P14 soil maps would be 3.44, 3.71, 1.41, 2.01, 3.57 and 0.10 Tg C, respectively. The simulated SOC dynamics are significantly influenced by map scales. Taking the total SOC changes based on the most detailed soil map, P005, as a reference, the relative deviation of P02, P05, P1, P4, and P14 were 7.9%, 58.9%, 41.6%, 3.9%, and 97.0%, respectively. Such differences are primarily attributed to missing soil types and spatial variations in soil types in coarse -scale maps. Although the relative deviation of P4 soil map for the entire Tai -Lake region is the lowest, substantial differences (i.e., 22-1010%) exist at soil subgroups level. Overall, soil map scale of P02 provides best accuracy for quantifying SOC dynamics of paddy soils in the study region. Considering the soil data availability of. entire China, P1 soil map is also recommended.This study suggested how to select an appropriate scale of input soil data for modeling the carbon cycle of agro-ecosystems. (C) 2016 Elsevier B.V. All rights reserved.

Understanding the impacts of climate change and agricultural management practices on soil organic carbon (SOC) dynamics is critical for implementing optimal farming practices and maintaining agricultural productivity. This study examines the influence of climatic variables and agricultural management on carbon sequestration potentials in Tai-Lake Paddy soils of China using the DeNitrification-DeComposition (DNDC, version 9.1) model, with a high-resolution soil database (1:50,000). Model simulations considered the effects of no-tillage, the application rates of manure, N fertilization, and crop residue, water management, and changes in temperature and precipitation. We found that the carbon sequestration potential in the top soils (0-30 cm) for the 2.32 Mha paddy soils of the Tai-Lake region varied from 4.71 to 44.31 Tg C under the feasible management practices during the period of 2001-2019. The sequestration potential significantly increased with increasing application of N-fertilizer, manure, conservation tillage, and crop residues, with an annual average SOC changes ranged from 107 to 121 kg C ha(-1) yr(-1), 159 to 326 kg C ha(-1) yr(-1), 78 to 128 kg C ha(-1) yr(-1), and 489 to 1005 kg C ha(-1) yr(-1), respectively. Toward mitigating greenhouse emissions and N losses, no-tillage and increase of crop residue return to soils as well as manure application are recommended for agricultural practice in this region. Our analysis of climate impacts on SOC sequestration suggests that the rice paddies in this region will continue to be a carbon sink under future warming conditions. Specifically, with rising air temperature of 2.0 degrees C and 4 degrees C, the average annual SOC changes were 52 and 21 kg C ha(-1) yr(-1), respectively. (C) 2016 Elsevier B.V. All rights reserved.

Agro-ecosystem models have been widely used to quantify soil organic carbon (SOC) dynamics based on digital soil maps. However, most of the studies use soil data of single or limited choices of map scales, thus the influence of map scales on SOC dynamics has rarely been quantified. In this study, six digital paddy soils databases of the Tai -Lake region in China at scales of 1:50,000 (P005),1:200,000 (P02),1:500,000 (P05), 1:1,000,000 (P1), 1:4,000,000 (P4), and 1:14,000,000 (P14) were used to drive the DNDC (DeNitrification & DeComposition) model to quantify SOC dynamics for the period of 2001-2019. Model simulations show that the total SOC changes from 2001 to 2019 in the top layer (0-30 cm) of paddy soils using P005, P02, P05, P1, P4, and P14 soil maps would be 3.44, 3.71, 1.41, 2.01, 3.57 and 0.10 Tg C, respectively. The simulated SOC dynamics are significantly influenced by map scales. Taking the total SOC changes based on the most detailed soil map, P005, as a reference, the relative deviation of P02, P05, P1, P4, and P14 were 7.9%, 58.9%, 41.6%, 3.9%, and 97.0%, respectively. Such differences are primarily attributed to missing soil types and spatial variations in soil types in coarse -scale maps. Although the relative deviation of P4 soil map for the entire Tai -Lake region is the lowest, substantial differences (i.e., 22-1010%) exist at soil subgroups level. Overall, soil map scale of P02 provides best accuracy for quantifying SOC dynamics of paddy soils in the study region. Considering the soil data availability of. entire China, P1 soil map is also recommended.This study suggested how to select an appropriate scale of input soil data for modeling the carbon cycle of agro-ecosystems. (C) 2016 Elsevier B.V. All rights reserved.

Using the DeNitrification-DeComposition (DNDC, version 9.5) model, we investigated the soil organic carbon (SOC) changes from 1980 to 2009 in Eastern China's upland-crop fields in northern Jiangsu Province. A currently most detailed high-resolution soil database, containing 17,024 polygons at a scale of 1:50,000, derived from 983 unique upland soil profiles, was used. A coarser county-level soil database was also used for a pair-wise simulation for comparison. We found that SOC changes modeled with the county-level soil database differ significantly from those with high-resolution soil data, with the deviation ranging from -64% to 8.0% in different counties. This implies that coarse soil data may lead to large biases in SOC simulation. With the high-resolution database, the model estimates a SOC increase of 37.89 TgC in the top soils (0-50 cm) over the study area of 3.93 Mha for the past three decades, with an average rate of 322 kg C ha(-1) year(-1). The SOC accumulation in the study region accounts for 10.2% of annual national carbon sequestration of upland soils, compared with the fraction of 3.7% in the total upland area of China. This underscores its significance to national climate mitigation. The annual SOC change varied between 61 to 519 kg C ha(-1) year mainly driven by the variations in N-fertilizer and manure applications. This study highlights the significance of high-resolution soil databases in quantifying SOC changes. Our high-resolution estimates of SOC will support farming and carbon management in this region. (C) 2016 Elsevier B.V. All rights reserved.

Understanding the impacts of climate change and agricultural management practices on soil organic carbon (SOC) dynamics is critical for implementing optimal farming practices and maintaining agricultural productivity. This study examines the influence of climatic variables and agricultural management on carbon sequestration potentials in Tai-Lake Paddy soils of China using the DeNitrification-DeComposition (DNDC, version 9.1) model, with a high-resolution soil database (1:50,000). Model simulations considered the effects of no-tillage, the application rates of manure, N fertilization, and crop residue, water management, and changes in temperature and precipitation. We found that the carbon sequestration potential in the top soils (0-30 cm) for the 2.32 Mha paddy soils of the Tai-Lake region varied from 4.71 to 44.31 Tg C under the feasible management practices during the period of 2001-2019. The sequestration potential significantly increased with increasing application of N-fertilizer, manure, conservation tillage, and crop residues, with an annual average SOC changes ranged from 107 to 121 kg C ha(-1) yr(-1), 159 to 326 kg C ha(-1) yr(-1), 78 to 128 kg C ha(-1) yr(-1), and 489 to 1005 kg C ha(-1) yr(-1), respectively. Toward mitigating greenhouse emissions and N losses, no-tillage and increase of crop residue return to soils as well as manure application are recommended for agricultural practice in this region. Our analysis of climate impacts on SOC sequestration suggests that the rice paddies in this region will continue to be a carbon sink under future warming conditions. Specifically, with rising air temperature of 2.0 degrees C and 4 degrees C, the average annual SOC changes were 52 and 21 kg C ha(-1) yr(-1), respectively. (C) 2016 Elsevier B.V. All rights reserved.