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Clear cell renal cell carcinoma (ccRCC) is the most common histological and devastating subtype of renal cell carcinoma. Necroptosis is a form of programmed cell death that causes prominent inflammatory responses. miRNAs play a significant role in cancer progression through necroptosis. However, the prognostic value of necroptosis-related miRNAs remains ambiguous. In this study, 39 necroptosis-related miRNAs (NRMs) were extracted and 17 differentially expressed NRMs between normal and tumor samples were identified using data form The Cancer Genome Atlas (TCGA). After applying univariate Cox proportional hazard regression analysis and LASSO Cox regression model, six necroptosis-related miRNA signatures were identified in the training cohort and their expression levels were verified by qRT-PCR. Using the expression levels of these miRNAs, all patients were divided into the high- and low-risk groups. Patients in the high-risk group showed poor overall survival ( P < 0.0001 ). Time-dependent ROC curves confirmed the good performance of our signature. The results were verified in the testing cohort and the entire TCGA cohort. Univariate and multivariate Cox regression models demonstrated that the risk score was an independent prognostic factor. Additionally, a predictive nomogram with good performance was constructed to enhance the implementation of the constructed signature in a clinical setting. We then employed miRBD, miRTarBase, and TargetScan to predict the target genes of six necroptosis-related miRNAs. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that 392 potential target genes were enriched in cell proliferation-related biological processes. Six miRNAs and 59 differentially expressed target genes were used to construct an miRNA–mRNA interaction network, and 11 hub genes were selected for survival and tumor infiltration analysis. Drug sensitivity analysis revealed potential drugs that may contribute to cancer management. Hence, necroptosis-related genes play an important role in cancer biology. We developed, for the first time, a necroptosis-related miRNA signature to predict ccRCC prognosis.

A wideband circularly polarized printed antenna is proposed and fabricated, which employs monofilar spiral stubs and a slit in the asymmetrical ground plane which are fed by an inverted L-shaped microstrip feedline. The CP operation is realized by embedding an inverted-L shaped strip and modified ground plane and can be markedly improved by loading monofilar spiral stubs asymmetrically connected at the edge of the ground plane. After optimization, the measured results of the finally structure demonstrate that a 10-dB bandwidth of 67.6% from 4.6 to 9.3 GHz and a 3-dB axial-ratio bandwidth (ARBW) for circular polarization (CP) of 60.1% from 5 to 9.3 GHz could be achieved which could completely cover the WLAN (5.725-5.85 GHz) band. Therefore, the proposed antenna is suitable for circular polarization applications in C band. To explain the mechanism of broadband circular polarization operation, the analysis of magnetic fields distributions and a parametric study of the design are given. Compared to other recent works, a simpler structure, wider axial ratio and impedance bandwidths and a more compact size are the key features of the proposed antenna.

A novel dual-band, dual-circularly polarized antenna is proposed and fabricated. The proposed antenna consists of an asymmetric U-shaped slot and an inverted L-shaped slot which are designed to excite two orthogonal E vectors with equal amplitude and 90° phase difference (PD), in addition, fed by a coplanar waveguide (CPW) Furthermore, a left-hand circular polarization in the direction of z > 0 and a right-hand circular polarization instead of the opposite direction both at the lower and upper bands are exhibited by the radiations of the antenna. Good agreement is achieved between the measurement and simulation, which indicates that a 10-dB bandwidth of 38.75% from 2.56 to 3.8 GHz and 21.8% from 10.01 to 12.53 GHz, while a 3-dB axial-ratio bandwidth (ARBW) of 13.4% from 2.77 to 3.2 GHz and 9.23% from 10.25 to 11.25 GHz at two operation bands, respectively, are covered in the designed antenna. To explain the mechanism of dual-band dual-circular polarization, the analysis of magnetic fields distributions and a parametric study of the design are given. Meanwhile, compared to other recent works, a single layer structure, wider axial ratio and impedance bandwidths and a more compact size are the key features of the proposed antenna.