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9 results on '"Adam Micheline"'

2. Inhibition of vascular smooth muscle cell proliferation and migration in vitro and neointimal hyperplasia in vivo by adenoviral-mediated atrial natriuretic peptide delivery

Author

Larifla, Laurent, Deprez, Isabelle, Pham, Isabelle, Rideau, Dominique, Louzier, Vanessa, Adam, Micheline, Eloit, Marc, Foucan, Lydia, Adnot, Serge, Teiger, Emmanuel, Université des Antilles (UA), Physiopathologie et Thérapeutiques Respiratoires, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10, Génétique Moléculaire et Cellulaire (UGMC), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, and École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], restenosis, natriuretic peptide, carotid artery, viruses, [SDV]Life Sciences [q-bio], atrial natriuretic peptide, cardiovascular system, gene transfer, intimal hyperplasia, hormones, hormone substitutes, and hormone antagonists, anp
Abstract

Chantier qualité GA; International audience; Background: Vascular smooth muscle cell (VSMC) proliferation and migration are important components of the remodeling process in atherosclerosis or following angioplasty. Atrial natriuretic peptide (ANP) inhibits the growth of VSMCs in vitro but this effect has not been proven in vivo. In the present study, we examined the effects of local overexpression of ANP following gene transfer on in vitro VSMC proliferation and migration and in vivo neointimal formation in a rat carotid artery model of vascular injury. Methods: ANP gene transfer was performed using a recombinant adenovirus containing the ANP cDNA controlled by the Rous sarcoma virus (RSV) long terminal repeat (Ad-RSV-ANP). A recombinant adenovirus expressing the RSV-controlled β-galactosidase gene (Ad-RSV-β-gal) was used as the control. Rat VSMC culture was used for in vitro studies. In the in vivo experiments, carotid arteries were analyzed after balloon injury and local infusion of the viral solution. Results: VSMCs transfected by Ad-RSV-ANP produced a significant amount of ANP detected by immunoreactive assay and accumulated about 6.5 times more cGMP than the viral control. VSMC proliferation stimulated with 10% fetal calf serum was reduced by 31% and migration by 25%. Fourteen days after injury, neointimal formation and the intima/media ratio were reduced by 25% and 28%, respectively, in the Ad-RSV-ANP-treated group compared to the control group. Conclusions: The present study demonstrates the efficacy of recombinant adenovirus Ad-RSV-ANP with respect to inhibiting rat VSMC proliferation and migration. Our findings also provide evidence that ANP is implicated in the modulation of vascular remodeling following endothelial injury.

Published
2012
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3. L’Europe du Nord : une nouvelle terre d’émergence pour les arboviroses ?

Author

Lara, Estelle, Breard, Emmanuel, Doceul, Virginie, Sailleau, Corinne, Viarouge, Cyril, DESPRAT, alexandra, Adam, Micheline, Chauveau, Emilie, VITOUR, Damien, Dominguez, Morgane, Zientara, Stephan, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Virologie UMR1161 (VIRO), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), and École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2012

4. Bluetongue virus dsRNA activate the innate antiviral response through the RLRs pathway

Author

Chauveau, Emilie, Adam, Micheline, Sailleau, Corinne, Breard, Emmanuel, Zientara, Stephan, Vitour, Damien, Virologie UMR1161 (VIRO), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), and École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011
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5. NS3 of bluetongue virus interferes with the innate antiviral response

Author

Vitour, Damien, Lara, Estelle, Chauveau, Emilie, Adam, Micheline, Sailleau, Corinne, Breard, Emmanuel, Zientara, Stephan, Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), International Cytokine Society. ITA., and Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011
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6. Role de la proteine NS3 du virus FCO dans l'immunité innée antivirale

Author

Chauveau, Emilie, Adam, Micheline, Sailleau, Corinne, Breard, Emmanuel, Viarouge, Cyril, Zientara, Stephan, Vitour, Damien, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2011

7. NS3 of bluetongue virus interferes with the innate antiviral response

Author

Lara, Estelle, DOCEUL, Virginie, Adam, Micheline, Sailleau, Corinne, Bréard, Emmanuel, Viarouge, Cyril, DESPRAT, Alexandra, Zientara, Stephan, VITOUR, DAMIEN, and Chauveau, Emilie

Subjects
Animal biology, Santé publique et épidémiologie, fièvre catarrhale ovine, Biologie animale, réponse immunitaire, interférence
Published
2011

8. Protection following intramuscular injection of replication-defective adenovirus vectored vaccines is linked to promoter efficiency in muscle representative cells

Author

Ambriović-Ristov, Andreja, Adam, Micheline, Monteil, Martine, Paulin, Denise, Eloit, Marc, and Vitale, Branko

Subjects
adenovirus-based vaccines, glycoprotein D, immune response, viruses
Abstract

To investigate the respective role of transduced cells in the induction of immune response following intramuscular inoculation of adenovirus-based vaccines, we generated several replication-defective adenoviruses expressing the glycoprotein D gene of pseudorabies virus (PRV) under the control of four different promoters : major late promoter of adenovirus type 2, human cytomegalovirus immediate-early promoter/enhancer (CMV), Rous sarcoma virus - long terminal repeat promoter, and human desmin gene 5' regulatory region (DES). All the adenovirus constructs were able to fully protect mice against challenge with PRV. The far most effective adenovirus constructs, on the criterion of protective doses and specific antibody response induction, were those in which the foreign gene was driven by the DES or CMV promoter. Wide variations in promoter strength in vitro were evidenced in several cell types representative of putative target cells following muscular inoculation (myoblasts, myotubes, fibroblasts, macrophages and endothelial cells). We confirmed previous findings that due to the E1A enhancer, only partial regulation of tissue-specific cellular promoters (i.e. DES) can be conferred, after introduction of the foreign gene cassette in the E1A region of adenovirus. The level of efficacy in vivo, was not correlated with the level of expression in myotubes, but paralleled the level of expression in endothelial cells and in myoblasts. These results suggest that, following adenovirus injection, locally produced cytokines may induce myoblasts to act as local antigen presenting cells.

Published
1998

9. PS1-105 Bluetongue virus dsRNA activate the innate antiviral response through the RLRs pathway

Author

Adam Micheline, Breard Emmanuel, Sailleau Corinne, Vitour Damien, Zientara Stephan, and Chauveau Emilie

Subjects
0303 health sciences, Immunology, Hematology, Biology, Biochemistry, Virology, Virus, 03 medical and health sciences, RNA silencing, 0302 clinical medicine, Immunology and Allergy, Molecular Biology, 030304 developmental biology, 030215 immunology
Published
2011
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