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The purpose of this study was to investigate whether polymorphisms in five microRNAs (miRNAs), miR-604A&gt, G, miR-608C&gt, G, 631I/D, miR-938G&gt, A, and miR-1302-3C&gt, T, are associated with the risk of idiopathic recurrent pregnancy loss (RPL). Blood samples were collected from 388 patients with idiopathic RPL (at least two consecutive spontaneous abortions) and 227 control participants. We found the miR-604 AG and AG + GG genotypes of miR-604, the miR-938 GA and GA + AA genotypes of miR-938, and the miR-1302-3CT and CT + TT genotypes of miR-1302-3 are less frequent than the wild-type (WT) genotypes, miR-604AA, miR-938GG, and miR-1302-3CC, respectively, in RPL patients. Using allele-combination multifactor dimensionality reduction (MDR) analysis, we found that eight haplotypes conferred by the miR-604/miR-608/miR-631/miR-938/miR-1302-3 allele combination, A-C-I-G-T, A-C-I-A-C, G-C-I-G-C, G-C-I-G-T, G-G-I-G-C, G-G-I-G-T, G-G-I-A-C, G-G-D-G-C, three from the miR-604/miR-631/miR-938/miR-1302-3 allele combination, A-I-G-T, G-I-G-C, G-I-A-T, one from the miR-604/miR-631/miR-1302-3 allele combination, G-I-C, and two from the miR-604/miR-1302-3 allele combination, G-C and G-T, were less frequent in RPL patients, suggesting protective effects (all p &lt, 0.05). We also identified the miR-604A&gt, G and miR-938G&gt, A polymorphisms within the seed sequence of the mature miRNAs and aligned the seed sequences with the 3′UTR of putative target genes, methylenetetrahydrofolate reductase (MTHFR) and gonadotropin-releasing hormone receptor (GnRHR), respectively. We further found that the binding affinities between miR-604/miR-938 and the 3′UTR of their respective target genes (MTHFR, GnRHR) were significantly different for the common (miR-604A, miR-938G) and variant alleles (miR-604G, miR-938A). These results reveal a significant association between the miR-604A&gt, A polymorphisms and idiopathic RPL and suggest that miRNAs can affect RPL in Korean women.

Additional file 1: Figure S1, related to Fig. 1, presents SAR analysis of the anti-proliferative effects of the MNPC derivatives. Figure S2, related to Fig. 2, presents MNPC dose-dependently blocks primary GBM sphere formation capacity and FDA-approved NTZ and TIZ exhibit selective inhibition against U87MG/EGFRvIII cells. Figure S3, related to Fig. 3, presents MNPC displays no inhibition against cell proliferation in normal cells and invasion or AEP enzyme in U87MG EGFRvIIII cells. Figure S4, related to Fig. 4, additionally shows that NQO1 and GSTP1 selectively mediate U87MG/EGFRvIII but not U87MG cell proliferation and oxidative stress. Figures S5 and Fig. S6 show that NQO1 and GSTP1 are highly positively correlated in GBM. Figure S7, related to Figs. 6 and 7, presents NQO1 and GSTP1 overexpression in U87MG cells and does not affect the parental U87MG proliferation. Figure S8, related to Fig. 8, presents MNPC suppresses tumor growth of U87MG/EGFRvIII cells and elongates the nude mouse life span. Table S1 provides information on binding affinities and inhibitory effects of MNPC with GSTP1 and its mutants. Table S2 presents crystallographic data and refinement statistics. Table S3 provides the primers used for mutagenesis. (PDF 3953 kb)

We investigated the mode of delivery and perinatal outcomes in low-risk pregnant women whose labor was electively induced or expectantly managed at term. Healthy women with viable, vertex singleton pregnancies at 37+0 to 40+6 weeks of gestation were included. Women electively induced (n = 416) in each week (37+0–37+6, 38+0–38+6, 39+0–39+6, 40+0–40+6 weeks) were compared with pregnant women with spontaneous labor (n = 487). The primary outcome was mode of delivery. A propensity score (PS) was derived using logistic regression to model the probability of elective induction group as a function of potential confounders. Altogether, 284 women with elective induction were matched with 284 women who underwent expectant management to create a PS-matched population. All analysis was performed using SAS software, version 9.4 (SAS Institute Inc., Cary, NC). All P values reported of the significance level was set at

Additional file 1: Table S1. Details of miRNA polymorphisms for PCR-RFLP analysis.

Supplementary_Tables_and_figure for Association Between miR-605A>G, miR-608G>C, miR-631I>D, miR-938C>T, and miR-1302-3C>T Polymorphisms and Risk of Recurrent Implantation Failure by Hyun Ah Lee, Eun Hee Ahn, Hyo Geun Jang, Jung Oh Kim, Ji Hyang Kim, Yu Bin Lee, Woo Sik Lee, and Nam Keun Kim in Reproductive Sciences