Search

We evaluated the metabolic and the nutritional aspects of 134 urolithiasis children in order to outline the characteristics of idiopathic urolithiasis in children. This prospective study group of 134 children (56 females, 78 males) with renal calculi was evaluated. The age range of the patients was six months to 16 years. A dietary survey was performed on every child. All patients were investigated with respect to stone localization and serum and urine risk factors. Statistical analysis of data was carried out using software SPSS 11.0 for Windows. Hypercalciuria was the most common risk factor detected in this group (28.3%). A decrease of water intake was noted in all age groups, especially in the rural area (549.6 mL/day vs. 1150.6 mL/day), and there was an increase in animal protein intake in 17 cases (mean: 1.9 g/kg). In addition, increased intake of starchy foods and food with high oxalate content (sorgum) were detected in the ten to 16 years age group (51%) of our study. Calcium oxalate monohydrate represents the principal component of idiopathic stone (58.2%), which is more frequent in children (68%) than in infants (51.7%) (P

Resume But Les etudes qui ont evalue l’influence de l’âge et du sexe sur la composition des calculs urinaires dans le monde arabe sont rares. L’objectif de cette etude a ete d’identifier la composition des calculs urinaires dans la population du centre tunisien et d’etudier leur evolution en fonction du sexe et l’âge. Patients et methodes Notre travail a porte sur 1200 patients lithiasiques tunisiens (729 de sexe masculin et 471 de sexe feminin) âges de six mois a 92 ans, qui ont ete admis dans les services d’urologie et de chirurgie pediatrique entre 2001–2010. L’analyse des calculs urinaires a ete realisee respectivement a l’aide d’un stereomicroscope et par spectroscopie infrarouge afin de determiner, respectivement, le type morphologique et de la composition moleculaire de chaque calcul. Resultats Nous avons observe une predominance de la lithiase renale qui a represente 48,6 % de l’ensemble des calculs. Les enfants et les personnes âgees ont ete les plus touches par la lithiase vesicale. L’analyse de la composition a montre que l’oxalate de calcium monohydrate (whewellite) etait majoritaire dans 51,8 % des calculs et dans 39,6 % des noyaux, sa frequence a diminue en fonction de l’âge de 61,4 % chez les jeunes adultes a 47,7 % chez les personnes âgees en faveur de l’augmentation des calculs d’acide urique (16,4 % et 35,6 % respectivement [ p Conclusion L’analyse de ces donnees a montre que les calculs urinaires en Tunisie avaient les memes caracteristiques physico-chimiques analogues a celles qui ont ete observees dans les pays developpes.

Background Studies that evaluate the effect of age on stone composition are scarce. The aim of this study was to highlight the changes in epidemiological characteristics (stone composition and location) of urolithiasis according to patients' age. Methods We studied 1,301 urolithiasis patients with age ranging from 6 months to 92 yr (781 males and 520 females). Stone analysis was performed using a stereomicroscope and infrared spectroscopy to determine the morphological type and molecular composition of each stone. Results The annual average incidence of new stone formation was 31.7 per 100,000 persons. In 71.8% of cases, calculi were located in the upper urinary tract. Compared to other age groups, children and old men were more affected by bladder stones. Calcium oxalate monohydrate was the most frequent stone component, even though its frequency decreased with age (59.5% in young adults and 43.7% in the elderly, P

Background Studies that evaluate the effect of age and gender on the stone composition are scarce. The aim of this study is to highlight the modification of epidemiological characteristics of this pathology according to patients’ sex and age. Patients and methods We studied 1269 urolithiasic patients ranging from 6 months to 92 years old and known as having urinary stones (752 males and 517 females). Stone analysis was performed respectively using a stereomicroscope and infrared spectroscopy to determine, respectively, the morphological type and molecular composition of each. Results The annual average incidence of new stone formation was 30.25 per 100,000 inhabitants. In 1041 patients (81%), calculi were located in the upper urinary tract. Children and old men were more affected by bladder stones. Calcium oxalate monohydrate remains the most frequent stone component even if its frequency fell according to age (63.2% in teenagers and 40.6% in the elderly [ p p Conclusion The analysis of these data shows that urinary stones in Tunisia are tending to evolve in the same direction as in industrialized countries.

Resumen Objetivos Los estudios que evaluan el efecto de la edad y el genero sobre la composicion de las piedras renales son escasos. El objetivo de este estudio es subrayar la modificacion de las caracteristicas epidemiologicas de esta patologia de acuerdo con el sexo y la edad de los pacientes. Material y metodos Estudiamos a 1.269 pacientes con urolitiasis de edades comprendidas entre 6 meses y 92 anos y que se conocia que tenian calculos urinarios (752 varones y 517 mujeres). Se realizo un analisis de las piedras usando un microscopio estereoscopico y espectroscopia infrarroja para determinar respectivamente el tipo morfologico y la composicion molecular de cada una. Resultados La incidencia anual promedio de formacion de nuevas piedras fue de 30,25 por cada 100.000 personas. En 1.041 pacientes (el 81%) los calculos estuvieron localizados en el tracto urinario superior. Los ninos y ancianos fueron mas afectados por piedras en la vejiga. El monohidrato de oxalato de calcio sigue siendo el componente mas frecuente de los calculos, a pesar de que su frecuencia caia en relacion con la edad (63,2% en adolescentes y 40,6% en ancianos [p Conclusion El analisis de estos datos muestra que los calculos urinarios en Tunez tienden a evolucionar en la misma direccion que en paises indrustrializados.

The epidemiology of renal stones has changed all over the world toward a predominance of calcium oxalate stones. However, studies evaluating the influence of age and gender on the distribution of the various types of urinary calculi are scarce. We will present the results of a study based on 205 calculi (from 122 boys and 83 girls) analyzed by infrared spectroscopy between 1993 and 2007. 90% of stones were collected by surgery, whereas only 6% by spontaneous passage. The biological exploration was performed in only 126 cases. Etiology was metabolic in 13.5%. 10.7% of stones were infectious, 13.1% were endemic and 54.9% were idiopathic. 7.8% were secondary to urinary stasis with malformation but no infection. Calcium oxalate (CaOx) was the predominant constituent in 54.7% of stones, followed by calcium phosphate and purines 14.6% in each of the cases. We found an increasing prevalence of calcium oxalate stones with age in both genders (42.9% in infant vs. 59.3% in child) (P < 0.05). Struvite stones were more frequent in infant than children. Purine stones were predominant in 20% of cases, but its prevalence decreases with age. The increase in calcium oxalate stone in school age children and the decrease in struvite and purine stones confirm the change on the etiology of urolithiasis according to age.

Resume Buts Les caracteristiques epidemiologiques sont importantes a prendre en consideration afin de cerner l’etiologie des enfants lithiasiques. Dans cette etude nous avons etudie la composition des calculs en fonction du sexe et de l’âge. Patients et methodes Notre travail portait sur 205 enfants lithiasiques tunisiens âges de trois mois a 16 ans, admis dans notre service entre 1993–2007. Un bilan metabolique urinaire, de premiere intention, a ete realise chez 126 patients. L’analyse physique et chimique des calculs a ete realisee respectivement par un stereomicroscope et par spectroscopie infrarouge. L’analyse statistique des resultats a ete faite a l’aide du logiciel SPSS 11.0. Resultats La localisation des calculs urinaires a ete vesicale dans 30,7 % des cas. Elle a ete plus frequente chez les nourrissons que chez les enfants de plus de dix ans ( p p Conclusion La prevalence plus importante des calculs d’oxalate de calcium et plus faible des calculs de purines chez les grands enfants a suggere la presence de facteurs de risque lithogenes differents en fonction de l’age. Une modification des habitudes alimentaires est probablement en cause.

KEY MESSAGE : Here, we describe a new developed quantitative real-time PCR method for the detection and quantification of a new specific endogenous reference gene used in GMO analysis. The key requirement of this study was the identification of a new reference gene used for the differentiation of the four genomic sections of the sugar beet (Beta vulgaris L.) (Beta, Corrollinae, Nanae and Procumbentes) suitable for quantification of genetically modified sugar beet. A specific qualitative polymerase chain reaction (PCR) assay was designed to detect the sugar beet amplifying a region of the adenylate transporter (ant) gene only from the species of the genomic section I of the genus Beta (cultivated and wild relatives) and showing negative PCR results for 7 species of the 3 other sections, 8 related species and 20 non-sugar beet plants. The sensitivity of the assay was 15 haploid genome copies (HGC). A quantitative real-time polymerase chain reaction (QRT-PCR) assay was also performed, having high linearity (R (2) 0.994) over sugar beet standard concentrations ranging from 20,000 to 10 HGC of the sugar beet DNA per PCR. The QRT-PCR assay described in this study was specific and more sensitive for sugar beet quantification compared to the validated test previously reported in the European Reference Laboratory. This assay is suitable for GMO quantification in routine analysis from a wide variety of matrices.

The clinical and biological characteristics of urolithiasis in children are still evolving in our country. Since the eighties no study has been conducted to determine incidence of urolithiasis in Tunisia.To define the current status of urolithiasis in Tunisian children.The records of 414 children with urolithiasis treated between 1983 and 2007 were found in a multi-centric study which took into consideration to age, sex, and history diagnosis and physical, laboratory, and radiologic findings. Clinical and biological date were performed in 344 cases. Stone analysis was performed by spectrophotometry.The incidence of paediatric urolithiasis has steadily decreased from 0.7/ 10 000 of the child population / year in 1991-1994 to 0.1 cases/10 000 children/ year in 2007. There was a continuous decrease in bladder stone over the past 25 years (47.1% in 1982-1986 Vs 10.2% in 2002-2007). Stones were homogeneous in 31.4% of cases. The main component was calcium oxalate stones in 36.4% of the cases. Since the eighties the increase of oxalates stones (15.6% in eighties Vs 51.5% actually) were with depends to purines and struvite stones.Epidemiologic characteristics of the urinary lithiasis (sex, age, localization and composition) have changed in Tunisia, due to the the improvements of standard of living. The occidentalization of the nutritional practices and the receding of the culinary traditions gave birth to new risks of urolithiasis characterized by the emergence of calcium oxalate stones.

We evaluated the metabolic and the nutritional aspects of 134 urolithiasic children, in order to outline the risk factors that contribute to idiopathic stone formation in children.In this prospective study 134 children (56 females, 78 males) with renal calculi were evaluated. The age of the patients ranged 6 months to 16 years. A dietary survey was performed on every child. All patients were investigated with respect to stone localization, serum and urine risk factors. Statistical analysis of data was carried out using software SPSS 11.0 for Windows. Statistical significance was determined using chi-square test.Hypercalciuria was the commonest risk factor detected in this group (28.3%). A decrease of water intake was noted in all age group specially in the rural area (549.6 vs 1150.6 ml/day), and an increase in animal protein intake in 17 cases (mean 1.9 g/kg). In addition, increased intake of starchy foods and food with high oxalate content (sorgum) was observed in our 10-16 years group (51%). Calcium oxalate monohydrate represents the principal component of idiopathic stone (58.2%), which is more frequent in children (68%) than infants (51.7%) (P0.02).The high frequency of idiopathic urolithiasis highlights the influence of dietary habit in stone formers in our country. The increase in calcium oxalate stones in school-age children confirms the change in the etiology of urolithiasis according to age.

Objective Since 1980s, the clinical and biological characteristics of urolithiasis in Tunisian children have continuously evolved. This retrospective study defines the current status of urolithiasis among children and adolescents in Tunisia. Methods We retrospectively reviewed the records of 310 children and adolescents (age: 3 months - 19 years) between 2003 and 2010, holding urolithiasis. A first-line metabolic, urine and plasma work-up was performed in all patients. Physical and chemical analysis of the stones was performed respectively by stereomicroscopy and infrared spectroscopy. Statistical analysis of the results was performed with SPSS 11.0 software. The Chi-square test was used for comparison of percentages. Findings Our study shows a male predominance of urolithiasis with a sex ratio of 1.5. Stones were located in the upper urinary tract in 70.7% of cases. Calcium oxalate was the predominant constituent in 52.6% of stones. There was an increasing prevalence of calcium oxalate stones according to age in both genders (48.6% in infants vs 68.5% in teenagers (P

Background: Stone composition has changed substantially over the past decades in developed countries. We studied urinary stone composition in Tunisian children. Methods: We studied 205 children ranging from 3 months to 16 years old age and who were diagnosed as having urinary stones (122 boys and 83 girls). Stone analysis was performed respectively using a stereomicroscope and by infrared spectroscopy to determine, respectively, the morphological type and molecular composition of each. Results: Of 205 stones analysed, 77 (37.5%) were composed of a single constituent. Calcium oxalate (CaOx) stone were predominant, being a constituent of 54.7% of stones, followed by calcium phosphate and purines; 14.6% of cases. Calcium oxalate monohydrate was more frequently seen in girls (p Conclusions: The increase in calcium oxalate stones in school age children and the decrease of struvite and purines stones confirm the change in the etiology of urolithiasis with respect to age.

Studies evaluating the influence of age and gender on the distribution of the various types of paediatric urinary calculi are scarce. Aim of our study was to high-light the modification of epidemiological characteristics of this pathology depending on patients' age and gender.We present the results of a study based on 205 calculi (from 122 boys and 83 girls) analyzed by infrared spectroscopy between 1993 and 2007. 54.6% of the patients were under 5 years.Calcium oxalate (CaOx) was the predominant constituent in 54.7% of stones, followed by calcium phosphate and ammonium urate (14.6% each). We found a male preponderance for struvite stones (12.3% vs 1.2%), and an increasing prevalence of calcium oxalate stones with age (42.9% in infants vs 59.3% in older children). Purines stones were observed in 20% of cases, but their prevalence decreases with age (28.6% in infants vs 18.5% in old children).The increase of calcium oxalate stone rate in school age children and the decrease of purines stones confirm the change on the etiology of urolithiasis according to age.

To show an outline of the clinical and biological characteristics of paediatric urolithiasis among Tunisian children in the coast region.This retrospective study included 168 children under the age of 16 years presented with urinary stones (100 boys and 68 girls). Patients were reviewed in a multi-centric study with regard to age at diagnosis, sex, history, and physical, laboratory, and radiologic findings. The physical and chemical analysis of stones was carried out respectively by a stereomicroscope and by infra-red spectroscopy.The sex ratio was 1.47. The clinical presentation of this pathology was dominated by abdominal pain (28%), hematuria (25.6%), dysuria (16.7%) and urinary tract infection (14.3%). Stones were located in the upper urinary tract in 75.6% of cases. Of the urine cultures, 14.3% were positive. Whewellite was more frequent in children stones than in infants (p0.05) and was the main component in 46.4% of stone section and 55.4% in stone surface.The male prevalence of paediatric urolithiasis is progressively decreasing in Tunisia. The epidemiological profile of renal stones in our country has changed towards a predominance of calcium oxalate stone and upper tract location.

Objective Studies evaluating the influence of age and gender on the distribution of the various types of pediatric urinary calculi are scarce. The aim of this study was to highlight the modification of epidemiological characteristics of this pathology according to patients' sex and age. Patients and methods A total of 205 calculi (from 122 boys and 83 girls) were analyzed by infrared spectroscopy between 1993 and 2007; 54.6% of the patients were under 5 years. Results Calcium oxalate was the predominant constituent in 54.7% of stones, followed by calcium phosphate and purines (14.6% each). We found a predominance of calcium oxalate in females (59.1% vs 50.8%), and a male preponderance for struvite stones (12.3% vs 1.2%). There was an increasing prevalence of calcium oxalate stones with age in both genders (42.9% in infants vs 59.3% in older children). Purine stones were predominant in 20% of cases, but prevalence decreased with age (28.6% in infants vs 18.5% in older children). Conclusion The increase in calcium oxalate stones in school-age children and the decrease in stones containing purines confirm a change in the etiology of urolithiasis according to age.

International studies may not reflect the real changes of stone disease in our own country. In this retrospective work, we describe the evolution of this pathology in Tunisia during the last 12 years.The records of 133 children with urolithiasis treated in Tunisia between 1994 and 2006 were reviewed. The physical and chemical analysis of stones was carried out respectively by a stereomicroscope and by infra-red spectroscopy and the epidemiologic data were collected.The male prevalence of this pathology is progressively declining. The male to female ratio, passed from 1.7 in the 1994-97 period to 1.5 in 2006. Upper urinary tract was most frequently affected by this pathology. Over the last 12 year period urinary tract infection was in continuous increase and currently reaches 50% being 21.9% in 1994. This explains the increasing frequency of struvite stones which passed from 15.6% in 1994 to 37.5% in 2006. Ammonium urate stones became more frequent in the last few years being the main component in 25% of cases (12.5% in 1994-97). Calcium oxalate monohydrate remains the most frequent stone component even if its frequency fell (59.4% in 1994 and 12.5% in 2006) in favour of the increase of phosphatic and phospho-calcic stones.The rise in the frequency of struvite and ammonium urate stone reflects an increase in the infectious risk factors in our country.

The clinical and laboratory characteristics of urolithiasis in Tunisian children have evolved continuously since the 1980s. This retrospective study defines the current status of urolithiasis in children in Tunisia.All 104 children (age: 8-192 months) of our series were hospitalized for urolithiasis. A first-line metabolic, urine and plasma work-up was performed in 96 patients. Physical and chemical analysis of the stones was performed by stereomicroscopy and infrared spectroscopy, respectively. Statistical analysis of the results was performed with SPSS 11.0 software. The Chi-square test was used for comparison of percentages.Our study shows a male predominance of urolithiasis with a sex ratio of 1.53. The clinical features were dominated by urinary tract infections (28.8%). Stones were situated in the upper tract in 75% of cases and the lower tract in 25% of cases. Urine culture was positive in 15 patients. Identification of the crystalline composition showed that whewellite was the most frequent crystalline species in children (80.0%) and infants (57.1%). Ammonium acid urate was more frequent in infants.In this study, we observed changes in the epidemiological characteristics (bladder stones and infection stones) of urolithiasis in children over the last decade in Tunisia. The patient's age is an important factor that must be taken into account during aetiopathogenic work-up.

Context: We will try to show an outline of the clinical and biological characteristics of pediatric urolithiasis among Tunisian children in the coastal region. Materials and Methods: This retrospective study included 168 children below 16 years (100 boys and 68 girls) presented with urinary stones. Patients were reviewed in a multi-centric study with regard to age at diagnosis, sexual, historical, physical, laboratory, and radiologic findings. The physical and chemical analysis of stones was carried out by a stereomicroscope and infra-red spectroscopy respectively. Statistical Analysis: Statistical analysis of data was carried out using software SPSS 11.0 for Windows. Statistical significance was determined using chi-square test. Results: The sex ratio was 1.47. Clinical presentation of this pathology was dominated by abdominal pain (28%), hematuria (25.6%), dysuria (16.7%) and urinary tract infection (14.3%). Stones were located in the upper urinary tract in 75.6% of cases. Of the urine cultures, 14.3% were positive. Whewellite is found more frequently in children stones than infants (P Conclusions: The male prevalence of pediatric urolithiasis is less obvious in Tunisia. Calcium oxalate is the most frequent chemical compound in Tunisian pediatric urolithiasis.