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Rhododendronluteum, Rhododendron cinsine dahil bir bitkiolup, bu cinse ait bitkiler geleneksel tedavide sıklıkla kullanılmaktadır. Rhododendron türlerinin sitotoksiketkilerini araştıran çok sayıda çalışma olmasına rağmen, R. luteum türünün sitotoksik etkilerini inceleyen çalışmalarsınırlıdır. Bu çalışmanın amacı R. luteumyaprak ekstraktının antioksidan özelliklerini ve insan kanser hücreleri üzerindekisitotoksik etkisini araştırmaktır. Ekstraktın toplam fenolik içeriği (TPC),toplam flavonoid içeriği (TFC) ve indirgeyici gücü spektrofotometrikprosedürler kullanılarak değerlendirildi. Ekstraktın beş kanser (insan meme,kolon, akciğer, prostat ve karaciğer karsinomu) ve insan fibroblast hücreleriüzerindeki sitotoksik etkisi MTT analizi kullanılarak belirlendi. EkstraktınTPC ve indirgeyici güç değerleri g örnek başına sırasıyla 173.2±3.97 mg gallikasit eşdeğeri ve 629.5±3.49 mg troloks eşdeğeri olarak bulundu. R. luteum yaprak ekstraktı normalfibroblast hücrelerine kıyasla özellikle kolon (1.9 kat) ve karaciğer (2.2 kat)kanser hücrelerine karşı seçici sitotoksisite sergiledi. Bu çalışma R. luteum yaprak ekstraktınınantiproliferatif etkisini ortaya koyan ilk çalışmadır. Ekstraktta bulunansitotoksik moleküllerin tanımlanması ve etki mekanizmalarının belirlenmesi içindaha kapsamlı çalışmalara ihtiyaç vardır., Rhododendron luteum belongs to the genus Rhododendron, membersof which are frequently used in folk medicine. Various studies haveinvestigated the cytotoxic effect of different Rhododendron species, butthere have been limited studies of the cytotoxic effect of R. luteum.The aim of this study was to investigate the antioxidant properties andcytotoxic effect of R. luteum leaf extract on the human cancer cells.The total phenolic content (TPC), total flavonoid content (TFC), and reducingpower of the extract were evaluated using spectrophotometric procedures. Thecytotoxic effect of the extract on five cancers (human breast, colon, lung,prostate, and liver carcinoma) and human fibroblast cells was determined usingthe MTT assay. TPC and reducing power values of extract were found 173.2±3.97mg gallic acid equivalents, and 629.5±3.49 mg trolox equivalents per to gsample, respectively. R. luteum leafextract exhibited selective cytotoxicity especially against colon (1.9 fold)and liver (2.2 fold) cancer cells compared to normal fibroblast cells. This isthe first study to reveal the antiproliferative effect of R. luteum leaf extract. Further studies are now needed to identifythe cytotoxic molecules in the extract and their mechanisms.

Oxidative stress comes out as a result of an imbalance between the reactive oxygen species (ROS) and antioxidant defenses. ROS besides damaging cellular molecules such as proteins and lipids, can also cause the formation of strand breaks and pyrimidine- and purine-derived lesions in DNA, thus leading to genome destabilization. To protect biomolecules against the attack of free radicals and/or to prevent the damage from happening, numerous natural and synthetic free radical scavengers and antioxidants have been developed and studied. Primula is a plant genius which comprises about 400 species. It is accepted as a good antioxidant source due to its phenolic contents. The aim of this study was to evaluate the protective effects of the water extract of Primula vulgaris on fibroblast cells exposed to oxidative agents by comet assay. Fibroblast cells (passage number is between 1 and 6) were pre-treated for 1 h with different concentrations (100, 250, and 500 µg/mL) of water extracts Primula vulgaris before the genotoxic agent. Then, the cells were treated with 20 µM H2O2 at 5 minutes and comet assay was performed. The water extracts of Primula vulgaris significantly decreased H2O2-induced DNA damage at the concentrations of 250 and 500 µg/mL (P

WOS: 000245874800007 PubMed: 17479644 Acute phase proteins are sensitive markers of tissue necrosis and inflammatory process. These markers may be especially useful in the neonatal period, in which mortality and morbidity rates are high, because fetus and baby are subjected to numerous metabolic, genetic, physiologic and environmental injuries such as neonatal asphyxia and septicemia. The purpose of the present study was to establish normal cord blood levels of some acute phase proteins in healthy term neonates. Umbilical cord blood was obtained at the time of vaginal delivery in 60 newborn infants (30 girls, 30 boys). Specific protein concentrations were measured by nephelometric assay. Transferrin, ceruloplasmin, alpha-1 antitrypsin, prealbumin, and alpha-2 macroglobulin concentrations [arithmetic mean (+/- SD)] were found to be 199.7 (+/- 34.6) mg/dl, 14.6 (+/- 4.0) mg/dl, 160.2 (+/- 23.6) mg/dl, 11.9 (+/- 2.2) mg/dl, and 284.6 (+/- 44.4) mg/dl, respectively. Prealbumin levels for girls [12.9 (+/- 2.2)] were found to be significantly higher than those of boys [10.9 (+/- 1.8)] (p

WOS: 000242740500015 PubMed: 17168134 We report on a rare hepatoid adenocarcinoma of the stomach producing alpha-fetoprotein (AFP) in five cases. Definitive features included an aggressive, invasive, and rapidly progressing neoplasm showing areas morphologically comparable to those of hepatocellular carcinomas. All patients had multiple metastases to lymph nodes and/or liver. The serum AFP level of the patients was between 83-87.900 ng/ml. Two subtotal and one palliative gastrectomy was performed. A short duration of chemotherapy was administered only in two patients. The length of survival averaged 4.7 months. Our experience together with what has been reported in literature suggest that the course of hepatoid adenocarcinoma of the stomach is more aggressive than an ordinary adenocarcinoma and that from a diagnostic point of view distinction from an adenocarcinoma may be accomplished histochemically and by measuring serum AFP levels.

WOS: 000451243800007, PubMed ID: 30595946, Rosa canina is a member of the genus Rosa that has long been used for medical objectives. Several studies have reported cytotoxic effects of different Rosa species, but there has been only limited investigation of the cytotoxic effect of R. canina. The purpose of the current study was to examine the potential effect of R. canina extract on cell viability, the cell cycle, apoptosis, and the expression of telomerase in human colon cancer (WiDr) cells. The cytotoxic effect of the extract was determined using MIT assay. The mechanism involved in the cytotoxic effect of the extract was then evaluated in terms of apoptosis and the cell cycle using flow cytometry. Mitochondrial membrane potential (MMP) was investigated using the fluorometric method, and expression levels of telomerase were studied using RT-PCR. R. canina extract exhibited a selective cytotoxic effect on WiDr cells compared with normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells. R. canina extract significantly repressed telomerase expressions at treatment times of 48 and 72 h in WiDr cells. Our results suggest that R. canina may have considerable potential for development as a novel natural product-based anticancer agent. (C) 2018 Xi'an Jiaotong University. Production and hosting by Elsevier B.V., Foundation of Scientific Research of Gumushane University [13.F5119.02.1], The authors would like to thank the Foundation of Scientific Research of Gumushane University for financially supporting this research under Project No: 13.F5119.02.1. The authors also wish to thank Professor Ersan Kalay from the Medical Biology Department, Karadeniz Technical University for professional assistance with the flow cytometry studies.

WOS: 000469066500001, PubMed ID: 31112051, Enriched in flavonoid compounds, phenol acids, and terpene derivatives, propolis has been shown to regulate apoptosis signaling pathways and alter the expression of microRNAs (miRNAs). In the present study, it has been aimed to examine the effects of Turkish propolis on miRNA levels of breast cancer (MCF-7) cells, and its relationship with cell proliferation and apoptosis. Cytotoxic activity of ethanolic propolis extract (EEP) was evaluated using MTT assay. Mechanisms involved in the cytotoxic action of Turkish propolis in MCF-7 cells were investigated with regard to apoptosis and cell cycle using flow cytometry and western blot. Mitochondrial membrane potential (MMP) were evaluated by spectrofluorometric method. miRNA levels were detected by qRT-PCR method. EEP exhibited selective toxicity against MCF-7 cells compared to normal fibroblast cells. EEP increased the cell cycle arrest at the G(1) phase. EEP elevated the apoptotic cell death through increasing pro-apoptotic protein levels (p21, Bax, p53, p53-Ser46, and p53-Ser15), decreasing MMP and altering the expression levels of specific tumor suppressors (miR-34, miR-15a, and miR-16-5p) and oncogenic (miR-21) miRNAs. These data support that Turkish propolis may be evaluated as a potential natural agent for new anticancer drugs in future., Karadeniz Technical University Scientific Research Foundation (Trabzon, Turkey) [2016-5680], This study was supported by the Karadeniz Technical University Scientific Research Foundation (Trabzon, Turkey) under project number 2016-5680.

Although some studies have evaluated thecytotoxic activity of different Fabaceaespecies, there has been only limited research into the cytotoxic effect of Dorycnium pentaphyllum. The purpose of this study wasto determine the antioxidant properties and cytotoxic effect of D. pentaphyllum extract on various humancancer cells. The total phenolic content (TPC) of the extract was determinedusing colorimetric method. The cytotoxic effect of the extract on human lung(A549), liver (HepG2), and breast (MCF-7) cancer cells and a normal humanfibroblast cells was assessed using the MTT assay. TPC value of extract wasfound as 140.3±1.1 mg gallic acid equivalent per g sample. Extract showedselective cytotoxicity on all studied cancer cells compared to normalfibroblast cells, and the IC50 values of the extract in the cancercells range from 100.4 to 298.5 μg/mL. This is the first study to reveal thecytotoxic effect of D. pentaphyllumextract on cancer cell lines. Phytomedical applications of D. pentaphyllum may represent promising approaches in the treatmentof cancer., Fabaceaeailesine mensup bitkilerin sitotoksik etkilerini konu alan bazı çalışmalarbulunmakla birlikte, Dorycnium pentaphyllum'unsitotoksik etkisi ile ilgili sınırlı araştırma bulunmaktadır. Bu çalışmanınamacı, D. pentaphyllum ekstraktının antioksidanözelliklerinin ve çeşitli insan kanser hücreleri üzerindeki sitotoksik etkisinibelirlenmesidir. Ekstraktın toplam fenolik içeriği kolorimetrik yöntemkullanılarak belirlendi. Ekstraktın insan akciğer (A549), karaciğer (HepG2) vememe (MCF-7) kanser hücreleri ile normal insan fibroblast hücreleri üzerindekisitotoksik etkisi MTT analizi kullanılarak değerlendirildi. Ekstraktın toplamfenolik içerikdeğerig örnekbaşına 140.3±1.1 mg gallik asit eşdeğeriolarak bulundu. Ekstraktın çalışılan tümkanser hücreserileri üzerindenormal fibroblast hücrelerine göre seçicisitotoksik etki gösterdiği belirlendi ve IC50değerleri100.4-298.5 μg/mL arasında hesaplandı. Bu çalışma D. pentaphyllum ekstraktının kanser hücre serileri üzerindeki sitotoksiketkisini ortaya koyan ilk çalışmadır. D.pentaphyllum'un fitokimyasal uygulamaları kanser tedavisinde umut vericiyaklaşımları temsil edebilir.

WOS: 000374779500018, PubMed ID: 26700423, Cancer is a heterogeneous disease, two of whose characteristic features are uncontrollable cell proliferation and insufficient apoptosis. Various studies have investigated the antiproliferative effects of propolis, a natural bee product, from different countries, and its cytotoxic effects have been attributed to its polyphenol contents. The purpose of this study was to show the cytotoxic effects, and possible mechanisms involved, of ethanolic extract of Turkish propolis (EEP) on the human lung cancer (A549) cell line. Cytotoxic activity of EEP on A549 cells was revealed using the MTT assay. Mechanisms involved in the cytotoxic action of EEP on A549 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, endoplasmic reticulum stress using RT-PCR, and caspase activity using luminometric analysis. EEP exhibited selective toxicity against A549 cells compared to normal fibroblast cells. We determined that EEP arrested the cell cycle of A549 cells at the G(1) phase, induced endoplasmic reticulum stress, caspase activity, and apoptosis and reduced mitochondrial membrane potential. These results indicate that Turkish propolis is capable of reducing cancer cell proliferation and may have a promising role to play in the development of new anticancer drugs in the future., Karadeniz Technical University Scientific Research Foundation (Trabzon, Turkey) [2007.114.001.3, 2008.114.001.5], This study was supported by the Karadeniz Technical University Scientific Research Foundation (Trabzon, Turkey) under project numbers 2007.114.001.3 and 2008.114.001.5.

WOS: 000403167900007, Objective: Cancer is one of the most deadly types of disease and evasion from apoptosis and unstoppable cell proliferation are accepted as its distinctive features. Many studies have evaluated the cytotoxic effect of different Morus species but, there is no study about cytotoxic effect of Morus rubra. In this study we aimed to evaluate phenolic composition, antioxidant properties and cytotoxic effect of acidified dimethyl sulfoxide extract of M. rubra (AMRE). Method: Antioxidant properties, phenolic composition and cytotoxic effect of AMRE were determined using spectrophotometric methods, HPLC, and MTT assay, respectively. Then, mechanisms of cytotoxic effect of AMRE on human prostate (PC-3) and lung (A549) cancer cells were examined in regard to cell cycle, apoptosis and mitochondrial membrane potential using flow cytometric methods. Results: Total phenolic content and reducing power values were 11.9 mg gallic acid equivalents and 42.9 mg trolox equivalents per g sample, respectively. Ascorbic and gallic acid were detected in AMRE as major antioxidant compounds. We determined that AMRE increased cell cycle arrest at G(1) phase and exhibited apoptotic features via decreasing mitochondrial membrane potential in both prostate and lung cancer cells. Conclusion: These findings demonstrate that M. rubra extract can affect the behavior of human prostate and lung cancer cells in vitro conditions, and this effect now needs to be investigated in vivo.

WOS: 000419309900011, Background: Primula vulgaris has been used in traditional treatment, and its biological functions are attributed to its polyphenolics content. Objectives: The aim of this study was to determine the phenolics composition and the antioxidant activity of water extract of P. vulgaris (WEP) and to determine its probable preventive effects against H2O2-induced DNA damage in human fibroblast cells. Methods: The total polyphenolic content (TPC), ferric reducing antioxidant power (FRAP), and radical scavenging activity of WEP were determined using spectrophotometric methods. Phenolic compounds and antigenotoxic effects of WEP were evaluated using HPLC and comet assay, respectively. Results: The TPC and FRAP values of WEP were 15.023 +/- 0.84 mg gallic acid and 82.63 +/- 0.31 mu M trolox per g sample, respectively. rho-coumaric acid and rutin were detected as major phenolics. Moreover, WEP reduced H2O2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to the positive controls (only 20 mu M H2O2 treatment). Conclusions: Primula vulgaris can be used in food, cosmetics, and drug industries because of its antioxidant and antigenotoxic activities., Foundation of Scientific Research of Karadeniz Technical University, The authors thank Dr. Huseyin Sahin (Giresun University) for his assistance with HPLC analysis. This work was supported by Foundation of Scientific Research of Karadeniz Technical University.

WOS: 000355931400005, Purpose: To investigate the total polyphenolic and flavonoid contents, antioxidant power and cytotoxic activity of ethanol extracts of Turkish propolis (EEP). Methods: The total polyphenolic and flavonoid contents of EEP were determined by spectrometric methods. Antioxidant power and cytotoxic activity of EEP were evaluated using ferric reducing antioxidant power (FRAP) and MTT assays, respectively. Results: The total polyphenolic and flavonoid contents, and FRAP value of EEP were 124.6 +/- 1.5 mg gallic acid/g sample dry weight, 42.0 +/- 0.8 mg quercetin/g sample dry weight and 311.0 +/- 2.5 mg trolox/g sample dry weight, respectively. EEP exhibited powerful cytotoxic effects against the five human cancer cell lines. The highest cytotoxic activity of Turkish EEP was demonstrated on PC-3 cell line (IC50 = 20.7 +/- 3.4 mu g/mL). Conclusion: The results demonstrate that EEP is a good source of antioxidant and a natural antitumor agent capable of reducing cancer cell proliferation., Foundation of Scientific Research of Karadeniz Technical University, Trabzon, Turkey [2007.114.001.3, 2008.114.001.5], This work was supported by Foundation of Scientific Research of Karadeniz Technical University (Project numbers: 2007.114.001.3 and 2008.114.001.5), Trabzon, Turkey.