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This project report describes the use of machine learning algorithms for sentiment analysis of Twitter data. The dataset is collected from Twitter using API key and pre-processed using various Natural Language Processing (NLP) techniques such as stop word removal, punctuation removal, tokenization, stemming, and lemmatization. The pre-processed data is then split into training and testing sets and subjected to feature extraction using count vectorization. Support Vector Machine (SVM) algorithm is implemented for classification and performance metrics such as accuracy, precision, recall, and F1-score are analyzed. The output is visualized using graphs and the project is implemented in a web application using Flask. The report discusses the existing and proposed systems for sentiment analysis, highlighting the advantages and disadvantages of using SVM algorithm. The methodology for model building and testing is also presented in detail. Additionally, the report presents a case study of Vader, a sentiment analysis tool, and its performance on Twitter data. The project report concludes with a discussion on the future scope of the project such as improving the accuracy of the model and incorporating real-time analysis. The report provides a comprehensive overview of sentiment analysis using machine learning algorithms on Twitter data, highlighting the importance and relevance of this field in today's digital age. Overall, the project report serves as a valuable resource for anyone interested in sentiment analysis and machine learning on social media data.

Objectives: Osteoporosis is a common skeletal disorder affecting postmenopausal women. Data suggest that postmenopausal women are at increased risk of periodontal diseases. Amino bisphosphonates are potent inhibitors of bone resorption and effectively used in the treatment of osteoporosis. Preliminary data indicate that there is a potential role for bisphosphonates in the management of periodontitis. Hence, this randomized placebo-controlled trial was designed to investigate the clinical efficacy of amino bisphosphonate on periodontal disease status among postmenopausal women. Materials and Methods: Thirty patients were randomly allocated to two treatment groups: Group A, which received scaling and root debridement and 70 mg weekly single oral dose of alendronate drug, and Group B, which received scaling and root debridement and placebo drug for 6 months. Clinical periodontal measurements were carried out for all patients at the baseline and 6 months later. Mandibular bone mineral density (BMD) was measured using a dual energy X-ray absorptiometer at the beginning of the study and the end of 6 months. Results: A weekly single oral dose of 70 mg alendronate was well-tolerated. The intragroup comparison showed significant improvement in periodontal parameters in both groups. The intergroup comparison showed a significant increase in BMD after 6 months in Group A when compared with Group B (P = 0.0179). Conclusion: Single oral dose of 70 mg alendronate per week is well-tolerable, gastro-intestinally safe, and improves the clinical outcome of nonsurgical periodontal therapy.

In this paper, a brain tumor segmentation method has been proposed. Image segmentation is required for the detection of brain tumors. For this purpose, brain images are divided into two distinct areas. This is one of the main but also the most complex element of the tumor identification process. A brain tumor is a result of mass of tissue that grows, it is the most important cause of the increased mortality rate among children as well as adults. This paper proposes a brain tumor segmentation method by using a combination of Adaptive Histogram Equalization and U-Net architecture. The performance of U-Net architecture and U-Net Architecture with Adaptive Histogram Equalization Technique are contrast by using PSNR (Peak Signal to Noise Ratio), MSE (Mean Squared Error), Quality and Complexity parameters. Overlap-tile strategy is used to smooth the segmentation of large images. Many MR images were collected, and the observations were carried out for statistical analysis using the Adaptive Histogram Equalization and U-Net architecture.

Langerhans’ cell histiocytosis (LCH) is a rare disease of the reticuloendothelial system in which there are abnormal proliferation and accumulation of histiocytes, abnormal cells deriving from bone marrow that can migrate from the skin to the lymph nodes. Langerhans’ cell histiocytosis has three variants: unifocal (eosinophilic granuloma), multifocal unisystem (Hand–Schuller–Christian triad), and multifocal multisystem (Letterer–Siwe disease). We present a case of oral lesions associated with LCH in a young male aged 15 years. The history, radiological appearance, histopathology, and treatment options of the patient are discussed. How to cite this article: Soangra R, Kapoor A, Meena D, et al. Langerhans’ Cell Histiocytosis Diagnosed through Periodontal Lesion in a 15-year-old Child: A Case Report. Int J Clin Pediatr Dent 2020;13(S-1):S115–S118.

A Pre-filled syringe is a disposable syringe that is supplied already loaded with the substance to be injected. It is a unit dose of parenteral medication to which a needle has been fixed by a producer. They are small which make them easy to carry and are dependable for delivering a precise dose of medication. These reasons are leading to growth of parenteral medication in pharmaceutical market. The intent of this review article is to provide information on regulatory guidelines involved in submission approach for marketing authorization of pre-filled syringe in developed region (USA, EU) and developing region (India). It also further explains about complexities associated with Pre-filled syringes in terms of regulations, submission approaches and quality development considerations in developing region. There is a need to develop an effective regulatory framework to make regulations of Pre-filled syringes more comprehensive for regulatory bodies. A harmonized forum to be made through which single application can be made and single review process can be followed to avoid deviations in regulatory pathways.

Bardet Biedl Syndrome (BBS) is a rare, heterogenous, ciliopathic autosomal recessive condition, affecting multiple organs. Primary features include polydactyly, obesity, mental retardation, hypogonadism, retinopathy and renal failure. Dental anomalies is regarded as secondary features, include hypodontia, microdontia, short roots and deep palate. Less than 15 cases have been reported from India. This article reports a case of Bardet- Biedl Syndrome in a male with typical systemic and oral features. This case exemplifies the need of multidisciplinary approach.

Health care practices that are not a part of a country’s tradition and are not integrated into the dominant health care system are called Complementary and Alternative Medicine. The most common Complementary and Alternative Medicine therapies include Acupuncture, Ayurveda medicine, Homeopathy, Naturopathy, chiropractic therapy, Herbal medicines, massage therapy, biofeedback, Hypnosis, Meditation, Deep breathing exercises, Yoga and Tai Chi. The term ‘‘periodontal disease,’’ refers to both gingivitis and periodontitis. Periodontitis follows gingivitis and is influenced by the individual’s immune inflammatory response which involves the destruction of the supporting structures of the teeth, including periodontal ligaments, bones, and soft tissues. Periodontitis demands serious concern because it is a direct cause of tooth mortality. Alternative medications, which are having many beneficial properties, are a good choice for the prevention or treatment of oral diseases without any side effects. In a developing country such as India, several people cannot even afford their daily basic expenses, and for them, these alternative medicines will be a boon.

Background Host modulation therapy has emerged as a new concept for the treatment of periodontal disease. Recently, a lot of research is being done in product containing docosahexaenoic acid (DHA) and eicosapentanoic acid (EPA). Omega-3 PUFA have therapeutic, anti-inflammatory and protective properties. This systematic review analysed the adjunctive use of omega-3 fatty acids in periodontal therapy of periodontitis patients. Methods PICO question (patient, intervention, comparison, and outcome) was formed. Keywords were generated and were fed in databases. The databases were Pubmed, Cochrane library and LIVIVO. Studies selected are Randomized clinical trial, clinical studies, and longitudinal studies. Meta –analysis were performed for Pocket depth (PD), Clinical attachment level (CAL), Gingival index (GI) and Plaque Index (PI). Risk of bias was also assessed. Results On analysis of all the 8 studies at 3 months showed significant effect of omega −3 fatty acid on clinical attachment level (CAL), Pocket depth (PD). There was significant effect of omega-3 fatty acids in 4 studies at 6 months. Conclusion Within the limitation of the review, omega- 3 polyunsaturated fatty acids seems to have a positive effect on periodontal healing following periodontal therapy. Chronic periodontitis patient should be counselled to incorporate omega −3 fatty acid in their diet along with standard periodontal therapy.

In medical image processing and analysis, segmentation is most compulsory assignment. In this paper, A multi-directional region growing approach is presented which use the concept of multiple seed selection to reduce the time consumption of region growing segmentation technique. The multiple seed selection concept works on the basis of eight-connected neighboring pixels. The attentiveness of the approach includes the selection of easiness of inceptive pixel and robustness to noises and the sequence of pixel execution. In order to choose a suitable threshold, the conception of neighboring difference transform (NDT) is presented for reducing the concern of threshold assortment issue. Exploratory outcome exhibits that this approach can acquire outstanding segmenting outcome; it will also not affect the result in the case of images with noises.

Rosuvastatin (RSV) is a new synthetic, hydrophilic statin with potent anti-inflammatory and osseodifferentiation actions. Autogenous bone graft (ABG) is still considered the gold standard in reconstructive bone surgery. Addition of platelet-rich fibrin (PRF) to ABG provides sustained release of various growth factors and facilitates survival of the graft.The study aims to clinically and radiographically compare the effectiveness of ABG and PRF with and without 1.2 mg RSV gel in the surgical treatment of intrabony defect in chronic Periodontitis patient.This was a randomized controlled clinical trial.Thirty-nine patients (one site per participant) with chronic periodontitis were randomly divided into three groups: Group 1 (open flap debridement [OFD] + placebo), Group 2 (OFD + ABG + PRF), and Group 3 (OFD + ABG + PRF + 1.2 mg RSV). Relative attachment level (RAL) and probing pocket depth (PPD) were recorded at baseline, 3, 6, and 9 months. Radiographic measurements such as defect height (A and B) and defect width (C) were calculated at baseline and 9 months.Intergroup comparison was done using Kruskal-Wallis ANOVA. An intragroup comparison was done using Friedman test and Wilcoxon signed-rank test.The mean PPD reduction and mean RAL gain were highly significant in Group 3 and Group 2 than Group 1. For Group 3, a significant reduction of defect height and width and a significant amount of bone fill were achieved than Group 2 and Group 1.Addition of 1.2 mg RSV gel, PRF, and ABG has synergistic effects, explaining their role as a regenerative material in the treatment of intrabony defects.

Background and aim. Dentinal hypersensitivity is a significant clinical problem encountered in daily dental practice. The management of this condition requires a good understanding of the complexity of the problem, as well as the variety of treatments currently available. The treatment approaches can be either home care products or professionally applied desensitizing agents. The present in-vitro study was designed to investigate the dentinal tubule occluding ability of commercially available nano HA containing mouthwash using FESEM analysis. Methods: In the present in -vitro study, 15 human premolars and canines were taken and sectioned mesiodistally. A total of 30 dentinal samples were obtained. All the dentinal discs were etched with 6% citric acid for 2 minutes. The treated samples were washed thoroughly with distilled water for 30 seconds. Samples were divided in two groups of 15 each. The specimens in Group I were shaken vigorously in the Vitis Sensitive mouthwash for 2 min twice daily for 14 days. After this intervention samples were placed in distilled water. Group II were immersed in distilled water. Samples were subjected to FESEM to analyze for tubular occlusion. Results. In group I nearly complete dentinal surface occlusion was present on the 7th and 14th day and precipitates were seen covering a large part of the dentinal surface. In group II no dentinal tubular occlusion was observed. Conclusion. The results of the present study support the ability of nHA containing Vitis sensitive mouthwash to occlude the dentinal tubules and thus it may demonstrate a significant reduction in dentinal hypersensitivity when used clinically.

Objective Periodontitis initiation and progression are a result of host immune inflammatory response to oral pathogens. Several pharmacological agents are being delivered locally, to improve periodontal health. Hence, the present randomized placebo controlled clinical trial is designed to check the clinical and antimicrobial efficacy of locally delivered 1.2% rosuvastatin (RSV) in intrabony defects (IBD) in periodontitis patients. Materials and Methods One-hundred patients were randomly allotted into two treatment groups: group A received 1. 2% RSV gel, scaling and root debridement and group B received placebo gel, scaling and root debridement. Clinical parameters, including modified sulcus bleeding index (mSBI), probing depth (PD), clinical attachment level (CAL), and plaque index (PI), were recorded at baseline before phase 1 and after 6 months. Radiographic assessment of IBD was done by cone beam computed tomography at baseline and after 6 months. Anaerobic colony count was done at baseline and after 180 days. Results On intragroup comparison, there is a significant improvement in periodontal parameters in both the groups. On intergroup comparison, there is significant gain in CAL in group A than group B (p = 0.04). There is significant decrease in PD in group A, compared to group B. There is significant bone fill in group A (p = 0.034), compared to group B. With respect to mSBI, PI, and anaerobic colony count, there is no significant difference between the two groups after 6 months. No adverse effect was noticed in any subjects. Conclusion The author concludes that 1.2% RSV gel when delivered locally into IBD improved periodontal clinical parameters such as PD and CAL and showed significant bone fill.

Previously, we showed that Rab5a and Rab5b differentially regulate fluid-phase and receptor-mediated endocytosis in Leishmania, respectively. To unequivocally demonstrate the role of Rab5b in hemoglobin endocytosis in Leishmania, we generated null-mutants of Rab5b parasites by sequentially replacing both copies of LdRab5b with the hygromycin and neomycin resistance gene cassettes. LdRab5b−/− null-mutant parasite was confirmed by qPCR analysis of genomic DNA using LdRab5b specific primers. LdRab5b−/− cells showed severe growth defect indicating essential function of LdRab5b in parasite. To characterize the role of Rab5b in Hb endocytosis in parasites, LdRab5b−/− cells were rescued by exogenous addition of hemin in growth medium. Our results showed that LdRab5b−/− cells are relatively smaller in size. Ultrastructural analysis revealed the presence of relatively enlarged flagellar pocket and bigger intracellular vesicles in these cells in comparison to control cells. Both promastigotes and amastigotes of Rab5b null-mutant parasites were unable to internalize Hb but fluid phase endocytosis of different markers was not affected. However, complementation of LdRab5b:WT in LdRab5b−/− cells (LdRab5b−/−:pRab5b:WT) rescued Hb internalization in these cells. Interestingly, LdRab5b−/− cells showed significantly less Hb-receptor on cell surface in comparison to control cells indicating a block in HbR trafficking. Finally, we showed that LdRab5b−/− parasites can infect the macrophages but are unable to survive after 96 h of infection in comparison to control cells. However, supplementation of hemin in the growth medium significantly rescued LdRab5b−/− Leishmania survival in macrophage indicating that LdRab5b function is essential for the acquisition of heme from internalized Hb for the survival of Leishmania.

Aim: A clinical trial to compare the relative efficacy of hydroxyapatite in combination with either PRF (Platelet rich fibrin) or collagen membrane in treatment of grade II furcation defects of mandibular molars. Methodology: The sample consisted of 35 Grade II mandibular molar furcation defects planed for open flap debridement (OFD) (Group 1, n=12), Platelet-rich fibrin membrane + Hydroxyapatite (HA) with OFD (Group 2, n=12), and Collagen membrane (CM) + HA with OFD (Group 3, n=11). These were recorded at baseline and 9 months postoperatively for plaque index (PI), gingival index (GI), probing depth (PPD), relative vertical and relative horizontal clinical attachment levels (RVCAL and RHCAL), and intrabony defect depth (IBD). Results: PI and GI scores decreased significantly in all three groups at 9 months postoperatively as compared to baseline scores. After 9 months of follow up visits mean PD reduction was greater in Group 2 (3.66 ± 0.98 mm) and Group 3 (3.45 ± 1.5 mm) than Group 1 (2.08 ± 0.90 mm), and mean RVCAL and RHCAL gain were greater in Group 2 (3.25 ± 0.75 mm and 3.92 ± 0.29 mm, respectively) and Group 3 (3.09 ± 0.70 mm and 3.89 ± 0.54 mm) compared with Group 1 (1.33 ± 0.77 mm and 2.25 ± 0.62 mm). A significant reduction in IBD depth was found in Groups 2 (2.16*0.38 mm) and 3 (1.58*0.35 mm) than in Group 1 (0.70*0.15 mm). The differences in PI, GI, PPD, RVCAL, and RHCAL between Groups 2 and 3 at baseline and 9 months were not statistically significant. There was a statistically significant difference in the depth reduction of IBD between Groups 2 and 3 at nine months. Conclusion: Within the scope of this study, it can be stated that the use of HA in conjunction with PRF or CM in the treatment of grade II mandibular furcation abnormalities resulted in a considerable reduction in GI PI, PPD, RVCAL, RHCAL, and IBD depth on follow up visit at 9 months after surgery. However, the combination of HA with PRF membrane exhibited significant improvement in radiographic parameters in comparison to the combination of HA with Collagen membrane.

Intracellular pathogens can modulate host Rabs and SNAREs to support their replication and immune evasion. Singh et al. show that the Salmonella effector SipA functionally mimics an R-SNARE and recruits host Q-SNAREs to promote membrane fusion. Thus, SNARE mimicry by this intracellular pathogen effector modulates the host trafficking machinery for Salmonella survival., SipA is a major effector of Salmonella, which causes gastroenteritis and enteric fever. Caspase-3 cleaves SipA into two domains: the C-terminal domain regulates actin polymerization, whereas the function of the N terminus is unknown. We show that the cleaved SipA N terminus binds and recruits host Syntaxin8 (Syn8) to Salmonella-containing vacuoles (SCVs). The SipA N terminus contains a SNARE motif with a conserved arginine residue like mammalian R-SNAREs. SipAR204Q and SipA1–435R204Q do not bind Syn8, demonstrating that SipA mimics a cognate R-SNARE for Syn8. Consequently, Salmonella lacking SipA or that express the SipA1–435R204Q SNARE mutant are unable to recruit Syn8 to SCVs. Finally, we show that SipA mimicking an R-SNARE recruits Syn8, Syn13, and Syn7 to the SCV and promotes its fusion with early endosomes to potentially arrest its maturation. Our results reveal that SipA functionally substitutes endogenous SNAREs in order to hijack the host trafficking pathway and promote Salmonella survival.

Status of bone-implant interface or osseointegration can be assessed by using resonance frequency analysis (RFA), which measures implant stability. A modified implant surface can significantly enhance osseointegration and reduce healing period. Platelet-rich fibrin (PRF) consists of fibrin mesh with entrapped platelets and leukocytes that release a huge number of growth factors which contribute to wound healing and tissue regeneration.The present study aims to evaluate the effect of PRF on osseointegration in terms of implant stability.This was a split-mouth randomized clinical trial.Sixty surgical sites were divided randomly into two groups. In Group 1 (thirty sites), PRF was placed in osteotomy sites before implant placement whereas no PRF was placed in Group 2 (thirty sites). Stability was measured using RFA in terms of implant stability quotient (ISQ) at baseline, 1 week, 1 month, and 3 months.Intergroup comparison was done using Mann-WhitneyOn intergroup comparison, Group 1 showed higher values for ISQ which were statistically significant (PRF has a significant effect on osseointegration of dental implants during the early healing period prior to loading.

Background and aim Dentinal hypersensitivity is a significant clinical problem encountered in daily dental practice. The management of this condition requires a good understanding of the complexity of the problem, as well as the variety of treatments currently available. The treatment approaches can be either home care products or professionally applied desensitizing agents. The present in-vitro study was designed to investigate the dentinal tubule occluding ability of commercially available nano HA containing mouthwash using FESEM analysis. Methods In the present in vitro study, 15 human premolars and canines were taken and sectioned mesiodistally. A total of 30 dentinal samples were obtained. All the dentinal discs were etched with 6% citric acid for 2 minutes. The treated samples were washed thoroughly with distilled water for 30 seconds. Samples were divided in two groups of 15 each. The specimens in Group I were shaken vigorously in the Vitis Sensitive mouthwash for 2 min twice daily for 14 days. After this intervention samples were placed in distilled water. Group II specimens were immersed in distilled water. Samples were subjected to FESEM to analyze for tubular occlusion. Results In group I nearly complete dentinal surface occlusion was present on the 7th and 14th day and precipitates were seen covering a large part of the dentinal surface. In group II no dentinal tubular occlusion was observed. Conclusion The results of the present study support the ability of nHA containing Vitis sensitive mouthwash to occlude the dentinal tubules and thus it may demonstrate a significant reduction in dentinal hypersensitivity when used clinically.

Differential functions of Rab5 isoforms in endocytosis are not well characterized. Here, we cloned, expressed, and characterized Rab5a and Rab5b from Leishmania and found that both of them are localized in the early endosome. To understand the role of LdRab5 isoforms in different modes of endocytosis in Leishmania, we generated transgenic parasites overexpressing LdRab5a, LdRab5b, or their dominant-positive (LdRab5a:Q93L and LdRab5b:Q80L) or dominant-negative mutants (LdRab5a:N146I and LdRab5b:N133I). Using LdRab5a or its mutants overexpressing parasites, we found that LdRab5a specifically regulates the fluid-phase endocytosis of horseradish peroxidase and also specifically induced the transport of dextran-Texas Red to the lysosomes. In contrast, cells overexpressing LdRab5b or its mutants showed that LdRab5b explicitly controls receptor-mediated endocytosis of hemoglobin, and overexpression of LdRab5b:WT enhanced the transport of internalized Hb to the lysosomes in comparison with control cells. To unequivocally demonstrate the role of Rab5 isoforms in endocytosis in Leishmania, we tried to generate null-mutants of LdRab5a and LdRab5b parasites, but both were lethal indicating their essential functions in parasites. Therefore, we used heterozygous LdRab5a(+/-) and LdRab5b(+/-) cells. LdRab5a(+/-) Leishmania showed 50% inhibition of HRP uptake, but hemoglobin endocytosis was uninterrupted. In contrast, about 50% inhibition of Hb endocytosis was observed in LdRab5b(+/-) cells without any significant effect on HRP uptake. Finally, we tried to identify putative LdRab5a and LdRab5b effectors. We found that LdRab5b interacts with clathrin heavy chain and hemoglobin receptor. However, LdRab5a failed to interact with the clathrin heavy chain, and interaction with hemoglobin receptor was significantly less. Thus, our results showed that LdRab5a and LdRab5b differentially regulate fluid phase and receptor-mediated endocytosis in Leishmania.

Speckle noise, which more commonly occurs in coherent imaging methods like as synthetic aperture radar, sonar, optical maser and ultrasound imaging, is undesirable final result of the image formation procedure in coherent imaging. In ultrasound imaging system, the presence of speckle results degradation in image quality and makes it tough for human interpretation and analysis. Consequently for examination of ultrasound images, speckle reduction is a very primary drawback. In the current era several methods for speckle suppression were proposed till now. Nevertheless in this paper, we've discussed model of speckle noise reduction with various filters that are basically used for speckle elimination. Different filters behave differently as few filters preserve the content material within the image and few filters provide smoothening process. Similarly some are better for edge detection and can be applied for tumor segmentation and so on. In the end, we have offered some regularly used filter assessment techniques.

The melanotic neuroectodermal tumor of infancy is an extremely rare, fast-growing but benign lesion, commonly occurring in the maxilla of children within the first year of life. Only about 380 cases of this particular tumor have been documented in the medical literature and very few of them have been reported to have occurred in late childhood. We describe here a relatively uncommon presentation of melanotic neuroectodermal tumor of infancy of maxilla arising from palatal gingiva of a 10 year-old female, its course and management by surgical excision with safe margins.

INTRODUCTION: Medical image segmentation is usually integrated as a critical step in medical image analysis, often associated with numerous clinical applications. Magnetic Resonance Imaging (MRI) provides detailed visualization of the various anatomical structures decisive for interventions and surgical plans.OBJECTIVES: The objective of this paper is to design and apply an enhanced brain tumor MRI segmentation using K-mean with K-means as machine learning based Particle Swarm Optimization (PSO) and Firefly Algorithm (FA). METHODS: A novel fitness function of Swarm Based PSO works on velocity variation is introduced, which enhances the segmented regions. The traditional k-means algorithm is enhanced by applying PSO to the segmented part. Another extension of Swarm Intelligence named Firefly is applied to compare the results of the PSO based segmentation, and Firefly based segmentation is used. RESULTS: The simulation results are evaluated in terms of precision (98%), recall (0.95), f-measure (0.96), accuracy (97%), and segmentation time (2.63s) to measure the image segmentation the quality of main results obtained.CONCLUSION: Comparative studies have shown that the proposed design using k-means combined with FA exhibited high accuracy and precision in detecting brain tumor RoI.

WRKY proteins are a large family of plant-specific transcription factors associated with regulation of biotic and abiotic stress responses, but how they respond to cereal rust pathogens has never been explored at the molecular level. Full-length cDNA of TaWRKY1B was obtained from a wheat cultivar HD2329 derivative containing leaf rust resistance gene Lr28 based on domain characteristics. The unique feature of this WRKY transcription factor gene was the close proximity of the DNA-binding domain and consensus DNA element W-Box within the open reading frame. Infection with a virulent race of leaf rust fungus resulted in 146-fold induction of the gene in resistant plants, but only 12-fold in the susceptible plants as compared with mock-inoculated controls. Docking models of 74 amino acids DNA-binding domain and 26 bp W-Box element showed that the WRKY domain, located on the β1 strand, only interacts with the W-Box at positions corresponding to W125, R126, K127 and Y128 amino acids. A truncated recombinant protein of 9.0 kD, encompassing the DNA-binding domain also showed binding specificity to the 32 bp W-Box element in electrophoretic mobility shift assays. The protein–DNA ensemble was also characterised using high-resolution atomic force microscopic imaging. The results contribute to an understanding of the molecular structure and function of a previously uncharacterised WRKY transcription factor in wheat that can be manipulated to improve biotic stress tolerance.