1. PPARγ ligands inhibit telomerase activity and hTERT expression through modulation of the Myc/Mad/Max network in colon cancer cells
- Author
-
Barbara Giglioni, Giuseppina Barrera, Angelo Cerbone, Piergiorgio Pettazzoni, Elisa Menegatti, Mario U. Dianzani, Carlo Ferretti, Stefania Pizzimenti, Berardi Daniela, Rosalba Minelli, and Cristina Toaldo
- Subjects
Telomerase ,Blotting, Western ,Peroxisome proliferator-activated receptor ,Myc/Mad/Max network ,Biology ,Ligands ,CaCo-2 cells ,Sp1 ,Proto-Oncogene Proteins c-myc ,Rosiglitazone ,15-deoxy-prostaglandin J2 ,Humans ,Telomerase reverse transcriptase ,RNA, Messenger ,Promoter Regions, Genetic ,Transcription factor ,PPARγ ligands ,chemistry.chemical_classification ,Regulation of gene expression ,Sp1 transcription factor ,Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ,Prostaglandin D2 ,Reverse Transcriptase Polymerase Chain Reaction ,telomerase activity ,Promoter ,Articles ,DNA, Neoplasm ,Cell Biology ,Molecular biology ,Telomere ,Gene Expression Regulation, Neoplastic ,PPAR gamma ,Repressor Proteins ,h-TERT ,chemistry ,GW9662 ,Colonic Neoplasms ,Molecular Medicine ,Thiazolidinediones ,Protein Binding - Abstract
In human cells the length of telomeres depends on telomerase activity. This activity and the expression of the catalytic subunit of human telomerase reverse transcriptase (hTERT) is strongly up-regulated in most human cancers. hTERT expression is regulated by different transcription factors, such as c-Myc, Mad1 and Sp1. In this study, we demonstrated that 15d-PG J2 and rosiglitazone (an endogenous and synthetic peroxisome proliferators activated receptor gamma (PPARgamma) ligand, respectively) inhibited hTERT expression and telomerase activity in CaCo-2 colon cancer cells. Moreover, both ligands inhibited c-Myc protein expression and its E-box DNA binding activity. Additionally, Mad1 protein expression and its E-box DNA binding activity were strongly increased by 15d-PG J2 and, to a lesser extent, by rosiglitazone. Sp1 transcription factor expression and its GC-box DNA binding activity were not affected by both PPARgamma ligands. Results obtained by transient transfection of CaCo-2 cells with pmaxFP-Green-PRL plasmid constructs containing the functional hTERT core promoter (including one E-box and five GC-boxes) and its E-box deleted sequences, cloned upstream of the green fluorescent protein reporter gene, demonstrated that 15d-PG J2, and with minor effectiveness, rosiglitazone, strongly reduced hTERT core promoter activity. E-boxes for Myc/Mad/Max binding showed a higher activity than GC-boxes for Sp1. By using GW9662, an antagonist of PPARgamma, we demonstrated that the effects of 15d-PG J2 are completely PPARgamma independent, whereas the effects of rosiglitazone on hTERT expression seem to be partially PPARgamma independent. The regulation of hTERT expression by 15d-PG J2 and rosiglitazone, through the modulation of the Myc/Max/Mad1 network, may represent a new mechanism of action of these substances in inhibiting cell proliferation.
- Published
- 2009