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We performed this study on the subterranean mole rat Nannospalax xanthodon (Nehring, 1898) in western Turkey and we analyzed karyotypes of 121 specimens from 54 localities. We determined that N. xanthodon has 2n = 36, NF = 70; 2n = 38, NF = 74; 2n = 40, NF = 72; 2n = 50, NF = 70; 2n = 50, NF = 74; 2n = 52, NF = 70; 2n = 56, NF = 72; and 6 different cytotypes of 2n = 60, NF = 74, 76, 78, 80, 82, 84 in the specimens analyzed from western Turkey. We flled most of the karyological gaps in western Anatolia and documented distributional areas of cytotypes. © TÜBİTAK.