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All organisms leave traces of their DNA in the environment they live in. This environmental DNA (eDNA) can be used to detect and monitor single species as well as communities, and potentially be utilized in early warning systems to detect alien species. In this report, we give an overview of eDNA-based methods used to detect and monitor alien species, and give examples of speciesspecific assays designed to identify species that are alien to Norwegian nature or have the potential to establish viable populations in Norway (doorknockers). We emphasize the need for several stages of testing before species-specific assays can be operational, and discuss the importance of including models to assess detection probabilities. Going through standards for sampling and analyses, we suggest a number of minimum requirements for eDNA sampling, laboratory practice, bioinformatics and the use of reference libraries, as well as for reporting results from eDNA studies. The origin and fate of eDNA in different environments can influence its usefulness in detecting and monitoring alien species. We outline factors for terrestrial, freshwater and marine ecosystems and provide examples from six case studies where eDNA has been used to detect and/or monitor alien invasive species: invertebrates and vascular plants in soil of imported ornamental plants, freshwater crayfish and crayfish plague, Gyrodactylus salaris, pink salmon, Canadian pondweed, and American lobster. We provide a decision diagram for detection and monitoring of invasive species starting with early considerations for implementation of a monitoring program, and ending with management decision points depending on detection outcomes at different stages. Finally, we provide some key recommendations for the use of eDNA in assessments of alien and doorknocker species. Bruk av miljø-DNA og DNA-baserte metoder for å vurdere og overvåke fremmede arter og dørstokkarter. The use of eDNA and DNA-based methods to assess and monitor alien and doorknocker species

Hyperspectral imaging (HSI) is a promising technology for environmental monitoring with a lot of undeveloped potential due to the high dimensionality and complexity of the data. If temporal effects are studied, such as in a monitoring context, the analysis becomes more challenging as time is added to the dimensions of space (image coordinates) and wavelengths. We conducted a series of laboratory experiments to investigate the impact of different stressor exposure patterns on the spectrum of the cold water coral Desmophyllum pertusum. 65 coral samples were divided into 12 groups, each group being exposed to different types and levels of particles. Hyperspectral images of the coral samples were collected at four time points from prior to exposure to 6 weeks after exposure. To investigate the relationships between the corals’ spectral signatures and controlled experimental parameters, a new software tool for interactive visual exploration was developed and applied, the HypIX (Hyperspectral Image eXplorer) web tool. HypIX combines principles from exploratory data analysis, information visualization and machine learning-based dimension reduction. This combination enables users to select regions of interest (ROI) in all dimensions (2D space, time point and spectrum) for a flexible integrated inspection. We propose two HypIX workflows to find relationships in time series of hyperspectral datasets, namely morphology-based filtering workflow and embedded driven response analysis workflow. With these HypIX workflows three users identified different temporal and spatial patterns in the spectrum of corals exposed to different particle stressor conditions. Corals exposed to particles tended to have a larger change rate than control corals, which was evident as a shifted spectrum. The responses, however, were not uniform for coral samples undergoing the same exposure treatments, indicating individual tolerance levels. We also observed a good inter-observer agreement between the three HyPIX users, indicating that the proposed workflow can be applied to obtain reproducible HSI analysis results. publishedVersion

Source at https://www.miljodirektoratet.no/publikasjoner/2021/juni-2021/kunnskapsstatus-for-bruk-av-molekylare-verktoy-i-kartlegging-og-overvakning-av-biologisk-mangfold-i-marine-miljo/ Denne rapporten er bestilt av Miljødirektoratet fra NorBOL (Norwegian Barcode of Life), et nasjonalt nettverk av forskningsinstitusjoner som koordineres av NTNU Vitenskapsmuseet. Universitetsmuseet i Bergen har hatt prosjektledelsen. Rapporten gir en oppsummering av dagens status på DNA-basert metodikk som verktøy i kartlegging og overvåking av biologisk mangfold i det marine miljø. Fokus har vært på innsamlingsmetodikk og protokoller for DNA-analyser av arter og artsgrupper. Metodikken er evaluert opp mot kartleggings- og overvåkingsaktivitet i regi av Miljødirektoratet, og kommer med anbefalinger for videre framdrift for å ta metodikken i bruk i nasjonal kartlegging og overvåking. Rapporten har også en gjennomgang og evaluering av eksisterende referansemateriale og referansesekvenser for relevante arter og artsgrupper som inngår i Miljødirektoratets kartleggings- og overvåkingsaktivitet.

Denne rapporten er bestilt av Miljødirektoratet fra NorBOL (Norwegian Barcode of Life), et nasjonalt nettverk av forskningsinstitusjoner som koordineres av NTNU Vitenskapsmuseet. Universitetsmuseet i Bergen har hatt prosjektledelsen. Rapporten gir en oppsummering av dagens status på DNA-basert metodikk som verktøy i kartlegging og overvåking av biologisk mangfold i det marine miljø. Fokus har vært på innsamlingsmetodikk og protokoller for DNA-analyser av arter og artsgrupper. Metodikken er evaluert opp mot kartleggings- og overvåkingsaktivitet i regi av Miljødirektoratet, og kommer med anbefalinger for videre framdrift for å ta metodikken i bruk i nasjonal kartlegging og overvåking. Rapporten har også en gjennomgang og evaluering av eksisterende referansemateriale og referansesekvenser for relevante arter og artsgrupper som inngår i Miljødirektoratets kartleggings- og overvåkingsaktivitet. publishedVersion

Project manager Hilde C. Trannum Mesocosm and bottle slurry experiments were conducted to assess and compare the effects of thermally treated cuttings (TCC) versus cuttings with water-based mud (WBM) on benthic communities. While WBM has been discharged for a long time, discharges of TCC have not yet been practised routinely. In a mesocosm experiment, cuttings were added in a layer thickness of 6.3 mm in box-core samples from the Oslofjord, and effects measured on benthic community structure, microprofiles of O2 and biogeochemical fluxes. In addition a bottle incubation experiment was performed on the same mud materials. Results from both experimental approaches showed significantly increased biodegradation measured as consumption of O2 and nitrate+nitrite in WBM and TCC treatments compared to controls. The biodegradation product ΣCO2 was released from WBM, but surprisingly consumed in TCC. This was presumably caused by precipitation of CaCO3(s) triggered by the mud ingredient Ca(OH)2(s) present in TCC. There was a significantly different impact on the benthic communities with mass mortality and reduction in macrofaunal biomass in TCC treatments, but unaltered faunal response in WBM exposure. The documented adverse effect of TCC cuttings was possibly due to intolerable alkaline conditions induced by the calcium oxide. Total E&P Norge AS and Norog

Knowlcdge status and -gaps in coral biology and scnsitivity to drilling opcrations of cold-watcr corals (CWC). as wcll as cxamples of rnitigation actions raken by oil operators were presentcd at the "'OLF coral workshop"· held in Stavanger. Norway, 31 st of May and Ist of June 2010. The workshop was organized by the Norwegian Oil lndustry Association (OLF) and hostcd by the International Rescarch Institute or Stavanger (IRIS). Acona Wellpro has supported IRIS and OLF in the finalizing of this report from the workshop. OLF

This study was made in the frame of an ongoing NFR project aiming at assessing the impact of drilling mud (DM) discharges from offshore O&G operations on the coldwater coral species Lophelia pertusa. Here, we have initiated developments and use of methodologies that could have a potential for application, at a later stage of their optimization, in field monitoring of coral health and hence for documenting the effects of drilling mud discharges

The two main objectives of our project was to study possible effects of drilling mud discharges on water column organism (plankton and fish), and filter feeding bivalves, and to analyse metals in fish and bivalves exposed to suspended particles of drilling mud to find out if metals from the mud was taken up by the organisms. The results from the different parts of the project have been reported in 8 manuscripts/reports. The ultimate aim is to publish as many of these manuscripts as possible. Hence this report is a collection of early versions of future publications.

Antibodies raised in rabbits against pure rat T-kininogen did not cross-react with lle-Ser-Bradykinin, bradykinin, nor with kininogens from other mammalian species. They presented a 1 to 15% cross-reaction with pure rat HMW kininogen depending on the quantity of HMW kininogen. A direct radioimmunoassay for rat T-kininogen in plasma was developed and it enabled 89 fmol of the protein to be detected. A good correspondence was obtained between the direct RIA and the T-kinin generating assay By the direct assay, it was found that T-kininogen is increased about ten fold in rats subcutaneously injected with turpentine. These data were confirmed by HPLC analysis of the plasma kinins released by trypsin which demonstrated that only T-kinins are increased, bradykinin being unchanged. it was possible according to the results obtained by the direct RIA and HPLC analysis to estimate that in the normal rat, HMW and LMW kininogen represent about 35 % and T-kininogen 65 %. In the turpentine-treated rat, T-kininogen reached 95%. This RIA will allow the study of the regulation of T-kininogen in the rat and the synthesis of this protein in cells in culture.