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Background: Both late-life depression (LLD) and short sleep duration increase the risk of cognitive impairment. Increased insular resting-state functional connectivity (FC) has been reported in individuals with short sleep duration and dementia. Objective: This study aimed to investigate whether short sleep duration is associated with impaired cognition and higher insular FC in patients with LLD. Methods: This case– control study recruited 186 patients with LLD and 83 normal controls (NC), and comprehensive psychometric assessments, sleep duration reports and resting-state functional MRI scans (81 LLD patients and 54 NC) were conducted. Results: Patients with LLD and short sleep duration (LLD-SS patients) exhibited more severe depressive symptoms and worse cognitive function than those with normal sleep duration (LLD-NS patients) and NC. LLD-SS patients exhibited higher FC between the bilateral insula and inferior frontal gyrus (IFG) pars triangularis than LLD-NS patients and NC, while LLD-NS patients exhibited lower FC than NC. Increased insular FC was correlated with short sleep duration, severe depressive symptoms, and slower information processing speeds. Furthermore, an additive effect was found between sleep duration and LLD on global cognition and insular FC. Conclusion: LLD-SS patients exhibited impaired cognition and increased insular FC. Abnormal FC in LLD-SS patients may be a therapeutic target for neuromodulation to improve sleep and cognitive performance and thus decrease the risk of dementia.

The rovibronic spectrum of PbS in the range of 19520−22900 cm−1 are investigated using the laser ablation-laser induced fluorescence method. The spectra in this range are assigned as the transitions of A- X and B- X according to the spectral analyzation. The upper electronic state of the transition in the range of 19520−22900 cm−1 is analyzed and discussed, and it is concluded that the upper state, A, is a mixture of [Formula: see text] and [Formula: see text] states, the [Formula: see text] state is in domination. The spectrum in the range of 22025−22900 cm−1 is assigned as the [Formula: see text] transition. The molecular constants of these two transitions are derived from the observed spectra. The Frank-Condon factors (FCFs) of these two transitions are also calculated using the RKR/LEVEL method. All the results are compared with the reported theoretical and experimental results, showing that the A state is a mixing state which is in consistent with Balasubramanian's relativistic configuration interaction calculation result. And our calculated FCFs are in agreement with our recorded spectra.

Immunogenic cell death (ICD) is a type of regulated cell death that can activate adaptive immune response, and its ability to reshape the tumor microenvironment via multiple mechanisms may contribute to immunotherapy. The treatment options for patients with skin cutaneous melanoma (SKCM) vary based on BRAF V600E statuses. However, all standard treatments include immunotherapy. Therefore, it is critical to identify ICD-associated signatures that can help classify patients according to benefits from ICD immunotherapy. In this study, data on melanoma samples with BRAF V600E mutation (BRAF V600E-mutant melanoma) and melanoma samples with wild-type BRAF V600E alleles (BRAF V600E WT melanoma) were collected from The Cancer Genome Atlas (TCGA) database. The ICD-related (ICD-high and ICD-low) subgroups of patients with BRAF V600E WT melanoma were established via consensus clustering. The analyses of survival, differentially expressed genes (DEGs), functional annotation, and immune landscape were performed in these two subgroups. Results showed that ICD-high subgroup was correlated with a positive overall survival (OS) and active tumor immune landscape. A model comprising seven prognosis ICD-related gene biomarkers was developed. Survival analysis and receiver operating characteristic (ROC) curve evaluation in both cohorts with BRAF V600E WT and BRAF V600E-mutant melanoma showed an accurate prognostic estimation of ICD-related risk signature. There was a correlation between immune cell infiltration and immunotherapy response and risk score. Thus, the ICD risk signature was closely associated with the tumor’s immune microenvironment. Our results may provide insights to further individualize and improve precision therapeutic decision-making in BRAF V600E-mutant and WT melanoma.

The comprehensive visual modeling of fluid motion has historically been a challenging task, due in no small part to the difficulties inherent in geometries that are non-manifold, open, or thin. Modern geometric cut-cell mesh generators have been shown to produce, both robustly and quickly, workable volumetric elements in the presence of these problematic geometries, and the resulting volumetric representation would seem to offer an ideal infrastructure with which to perform fluid simulations. However, cut-cell mesh elements are general polyhedra that often contain holes and are non-convex; it is therefore difficult to construct the explicit function spaces required to employ standard functional discretizations, such as the Finite Element Method. The Virtual Element Method (VEM) has recently emerged as a functional discretization that successfully operates with complex polyhedral elements through a weak formulation of its function spaces. We present a novel cut-cell fluid simulation framework that exactly represents boundary geometry during the simulation. Our approach enables, for the first time, detailed fluid simulation with "in-the-wild" obstacles, including ones that contain non-manifold parts, self-intersections, and extremely thin features. Our key technical contribution is the generalization of the Particle-In-Cell fluid simulation methodology to arbitrary polyhedra using VEM. Coupled with a robust cut-cell generation scheme, this produces a fluid simulation algorithm that can operate on previously infeasible geometries without requiring any additional mesh modification or repair.

Same-style products retrieval plays an important role in e-commerce platforms, aiming to identify the same products which may have different text descriptions or images. It can be used for similar products retrieval from different suppliers or duplicate products detection of one supplier. Common methods use the image as the detected object, but they only consider the visual features and overlook the attribute information contained in the textual descriptions, and perform weakly for products in image less important industries like machinery, hardware tools and electronic component, even if an additional text matching module is added. In this paper, we propose a unified vision-language modeling method for e-commerce same-style products retrieval, which is designed to represent one product with its textual descriptions and visual contents. It contains one sampling skill to collect positive pairs from user click log with category and relevance constrained, and a novel contrastive loss unit to model the image, text, and image+text representations into one joint embedding space. It is capable of cross-modal product-to-product retrieval, as well as style transfer and user-interactive search. Offline evaluations on annotated data demonstrate its superior retrieval performance, and online testings show it can attract more clicks and conversions. Moreover, this model has already been deployed online for similar products retrieval in alibaba.com, the largest B2B e-commerce platform in the world., Comment: Accepted in The Web Conference (WWW2023) Industry Track. 5 pages, 3 figures

Tetrazole compounds are a category of energetic materials with great potential. Their stability is inherently related to their aromatics. On the basis of research in DFT at b3lyp/6-311** level on three compounds interested, viz. 5-amino-1H-tetrazole, 5-Carboxyl-1H-tetrazole, 2-methyl-5-Carboxyl-tetrazole, their aromatic characters are extensively analyzed by virtue of different estimation method. Analyses show that these methods, NICS, ICSS, AdNDP, π-electrons density and LOL-pi, are efficient for substituted-tetrazole ring aromaticity estimation. Conclusion denotes that both substituent and substituted position can significantly affect the aromaticity of tetrazole ring of tetrazole compound. Among these given substituents, electro-withdrawing group -COOH significantly increases the aromaticity of the tetrazole rings due to the withdrawing to π-electrons thereof but electro-donating group -NH2 weakens the one of the tetrazole rings due to the donating to π-electrons thereof. But for 2-methyl-5-Carboxyl-tetrazole, the aromaticity increasing of -COOH imposed on π-electrons of tetrazole ring thereof is partially balanced by the existence of the electron-donating group -CH3 at asymmetric meta-position of tetrazole ring. Consequently, the aromaticity order is 5-Carboxyl-1H-tetrazole > 2-methyl-5-Carboxyl-tetrazole > 5-amino-1H-tetrazole.

Excessive inflammatory response caused by infiltration of a large number of neutrophils is one of the important features of acute lung injury(ALI)/acute respiratory distress syndrome (ARDS). Lipoxin A4 (LXA4) is an important endogenous mediator in the process of inflammation resolution, which has a strong role in promoting inflammation resolution. In this study, we examined the impact of LXA4 on the pulmonary inflmmatory response and the neutrophil function in a rat model of ARDS. Our results indicated that Exogenous administration of LXA4 could reduce the degree of lung injury in ARDS rats and inhibit the release of pro-inflammatory factors TNF- α and IL-1β in lung tissue homogenate. However, LXA4 has no lung protective effect on ARDS rats with neutrophil depletion in rats, nor can it inhibit the levels of pro-inflammatory factors TNF-α and IL-1β in lung tissue homogenate. LXA4 can inhibit the production of reactive oxygen species (ROS) and NETs in peripheral blood neutrophils of ARDS rats. At the same time, LXA4 can promote the phagocytosis of neutrophils in ARDS rats in vitro, and can also promote the apoptosis of neutrophils in ARDS rats and reduce neutrophil NETosis. In addition, the effect of LXA4 on the function of peripheral blood neutrophils in ARDS rats is mediated by its receptor ALX. LXA4 can inhibit the release of NE and MPO from neutrophils, thereby inhibiting the production of NETs, and the effect of LXA4 can be comparable to NEi and MPO inhibitors. In summary, these findings indicate that LXA4 has a protective effect on LPS-induced ARDS rats by affecting the function of neutrophils.Summary sentenceLXA4 has protective effect on LPS induced ARDS rats by affecting the function of neutrophils.

We model civil litigation as a simultaneous contest between a plaintiff and a defendant who have monetary and emotional preferences. The litigants’ emotional variables capture a non-monetary joy of winning and relational emotions toward each other. A contest success function (CSF) describes the litigants’ respective probabilities of success based on their endogenous litigation expenses and exogenous relative advantages. The model does not specify a functional form for the CSF. Instead, it accommodates any CSF that satisfies general and intuitive assumptions, which capture frequently-used functional forms. A cost-shifting rule allows the winner to recover an exogenous proportion of her litigation expenses from the loser. There exists a unique Nash equilibrium with positive expenses. In equilibrium, negative relational emotions (but not a positive joy of winning) amplify the effects of cost shifting, and vice versa. Thus negative relational emotions and positive cost shifting have a similar strategic role, and one can be a substitute for the other. If the litigants’ relative advantages are sufficiently balanced, then more cost shifting (or more negative relational emotions) increases total expenses in equilibrium.

Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive. Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied. Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin were determined to be 2.90 × 10

Poor visible light utilization and charge separation efficiency of TiO2 restrict its extensive application in the photocatalytic field. Herein, a specific Z-scheme TiO2-x/Cu/ZnO heterojunction was successfully constructed by atomic layer deposition (ALD) technique and spray pyrolysis technology. Benefited from the surface plasmon resonance (SPR) effect of Cu and Z-scheme heterojunction, the visible light absorption capacity was greatly enhanced. Meanwhile, ZnO nanolayer coating, prepared by ALD technique, protects Cu element to hinder its oxidation, thus enhancing the separation efficiency of photogenerated carriers. Therefore, the photocatalytic hydrogen production performance was significant improved, exhibiting a maximum value of 342.0 µmol·g−1·h−1 for the optimal B-T-0.1C-10Z (black TiO2/0.1Cu/10 nm ZnO) sample without any noble-metal cocatalyst, which is higher than pure TiO2 (310.7 µmol·g−1·h−1, with 3 wt% Pt) synthesized by spray pyrolysis method under equal conditions. In addition, a possible mechanism for the enhanced performance was briefly discussed based on the experimental results.

Background: The olfactory system is affected very early in Alzheimer’s disease and olfactory loss can already be observed in patients with mild cognitive impairment (MCI), an early stage of AD. Objective: The aim of this randomized, prospective, controlled, blinded study was to evaluate whether olfactory training (OT) may have an effect on olfactory function, cognitive impairment, and brain activation in MCI patients after a 4-month period of frequent short-term exposure to various odors. Methods: A total of 38 MCI outpatients were randomly assigned to OT or a control training condition, which were performed twice a day for 4 months. Olfactory testing, comprehensive neuropsychological assessment, and a passive odor perception task based on magnetic resonance imaging were performed before and after training. Results: The results suggested that OT exhibited no significant effect on olfaction and cognitive function. Additionally, OT exhibited a positive effect on frontal lobe activation (left middle frontal gyrus and orbital-frontal cortex) but exhibited no effect on grey matter volume. Moreover, the change of olfactory scores was positively associated with the change of frontal activation. Conclusion: OT was found to have a limited effect on olfaction and cognition in patients with MCI compared to a non-OT condition but increased their functional response to odors in frontal area.

A new sodium borate-formate, which displays a pcu-type open framework and ultraviolet nonlinear optical properties, has been synthesized under surfactant-thermal conditions.

We propose a general convex optimization problem for computing regularized geodesic distances. We show that under mild conditions on the regularizer the problem is well posed. We propose three different regularizers and provide analytical solutions in special cases, as well as corresponding efficient optimization algorithms. Additionally, we show how to generalize the approach to the all pairs case by formulating the problem on the product manifold, which leads to symmetric distances. Our regularized distances compare favorably to existing methods, in terms of robustness and ease of calibration., Comment: 11 pages (excluding supplementary material), 14 figures, SIGGRAPH 2023

We propose a novel Moebius interpolator that takes as an input a discrete map between the vertices of two planar triangle meshes, and outputs a smooth map on the input domain. The output map interpolates the discrete map, is continuous between triangles, and has low quasi-conformal distortion when the input map is discrete conformal. Our map leads to considerably smoother texture transfer compared to the alternatives, even on very coarse triangulations. Furthermore, our approach has a closed-form expression, is local, applicable to any discrete map, and leads to smooth results even for extreme deformations. Finally, by working with local intrinsic coordinates, our approach is easily generalizable to discrete maps between a surface triangle mesh and a planar mesh, i.e., a planar parameterization. We compare our method with existing approaches, and demonstrate better texture transfer results, and lower quasi-conformal errors.

Nasopharyngeal carcinoma (NPC) is a malignant epithelial tumor originating in the nasopharynx and has a high incidence in Southeast Asia and North Africa. To develop these comprehensive guidelines for the diagnosis and management of NPC, the Chinese Society of Clinical Oncology (CSCO) arranged a multi‐disciplinary team comprising of experts from all sub‐specialties of NPC to write, discuss, and revise the guidelines. Based on the findings of evidence‐based medicine in China and abroad, domestic experts have iteratively developed these guidelines to provide proper management of NPC. Overall, the guidelines describe the screening, clinical and pathological diagnosis, staging and risk assessment, therapies, and follow‐up of NPC, which aim to improve the management of NPC.

To improve the performance of Mo-based cermets, Mo-(Ni)-Al2O3 cermets were prepared with raw materials of nano-sized Al2O3 powder. The effects of Al2O3 and Ni additions on the densification behavior, microstructure, bending strength, and hardness of the Mo-(Ni)-Al2O3 cermets were experimentally analyzed. The results showed the content of Al2O3 had a great influence on the degree of densification of Mo-Al2O3 cermets at the sintering temperature of 1600°C. In addition, the Al2O3 particles were distributed evenly in the matrix of Mo-based cermets, which refined the grains of Mo and increased the hardness of cermets. Because of the weak interfacial bonding strength between Al2O3 ceramic phase and Mo metal phase, the bending strength decreased continuously with increasing Al2O3 content. However, Ni addition improved the wetting and adhesion of Al2O3 and Mo. With the addition of Ni, the relative density increased, and thus hardness and bending strength were improved.

BackgroundThis study aimed to develop a vaccine that targets mutation-derived neoantigen in Chinese non-small-cell lung cancer (NSCLC).MethodsA cohort of 1862 Chinese NSCLC patients who underwent targeted sequencing with a 1021-gene panel was investigated. HLA typing was done using OptiType v1.0 and neoantigens were predicted by netMHCpan v4.0. HLA LOH was inferred using the lohhla algorithm and TMB were quantified by counting the total number of non-synonymous ones based on our panel data. CIBERSORT was utilized to estimate the TME in different EGFR mutant subtype by using TCGA data.ResultsHLA-A*11:01(42.59%) was the top one allele and HLA-A*33:03(12.94%) ranked 12th. EGFR L858R (22.61%) was the most prevalent gene variant. The binding affinity (IC50 MT = 22.9 nM) and shared frequency (2.93%) of EGFR L858R in combination with HLA-A*33:03 were optimal. In a subsequent further analysis on immunological features of EGFR mutant subtypes, 63.1% HLA loss of heterozygosity LOH (HLA LOH) and 0.37% (7 of 1862) B2M aberrations were found in our population, both had no significant association with EGFR mutant subtypes suggesting that the process of antigen presentation involved HLA LOH and B2M mechanisms in EGFR L858R is working. Tumor mutation burden (TMB) was investigated by utilizing our panel and showed that EGFR L858R had the lowest TMB compared with other EGFR mutant subtypes. In addition, analysis of 22 immune cell types from The Cancer Genome Atlas (TCGA) data showed EGFR L858R was correlated with low level of CD8 T cells, activated CD4 memory T cells and elevated level of macrophage M2 suggesting an inhibited tumor microenvironment (TME).ConclusionOur study identified that EGFR L858R neoantigen had the potential to generate cancer vaccines in NSCLC patients with HLA A*33:03. The neoantigen-based vaccines may become an effective salvage regimen for EGFR L858R subgroup after targeted therapy or immune checkpoint inhibitors (ICIs) failure.

Leaf functional traits in drylands are sensitive to environmental changes, which are closely related to plant growth strategies and resource utilization ability and can reflect the balance of substance synthesis and water loss. However, the influence of environmental factors on photosynthetic production traits and water use traits is still unclear in drylands. In this study, nine environmental factors (climatic characteristics and soil physical and chemical properties), leaf net photosynthetic rate (A), transpiration rate (E), and stomatal conductance (GSW) were measured via 60 plant samples and 45 soil samples, which were collected at five sampling sites according to rainfall gradient. Redundancy analysis (RDA), structural equation model (SEM), and regression analysis were used to analyze the influencing mechanism of drought on photosynthetic production traits and water use traits. The results provided the following conclusions: (i) The hydrothermal condition determined A, E, and GSW by affecting the spatial distribution of soil nutrients (SN) and soil salinity (SS); meanwhile, temperature was able to affect A, E, and GSW directly. (ii) The water content (WC) was the key driver of the strength of the synergistic relationship between photosynthetic production traits and water use traits; soil salinity (SS) was the main driver of the synergistic relationship between E and GSW.

Carboxymethyl-β-cyclodextrins (CM-β-CDs) with five kinds of degrees of substitution were synthesized and characterized. Analytical enantioseparation of six basic drugs containing N-alkyl groups, including pheniramine, chlorpheniramine, labetalol, propranolol, venlafaxine, and trans-paroxol, was achieved by reversed-phase high-performance liquid chromatography (RP-HPLC) using the synthesized CM-β-CD as chiral mobile phase additives. Key influence factors were optimized, including organic modifier, pH value, CM-β-CD with different degrees of substitution, and concentration of CM-β-CD. The mobile phase was composed of methanol and 10 mmol L

Background Subjective cognitive decline (SCD) is a putative Alzheimer’s disease (AD) precursor without objective neuropsychological deficits. The hippocampus plays an important role in cognitive function and emotional responses and is generally aberrant in SCD. However, previous studies have mainly focused on static functional connectivity (sFC) by resting-state functional magnetic resonance imaging (fMRI) in SCD individuals, and it remains unclear whether hippocampal dynamic functional connectivity (dFC) changes exist in SCD and whether those changes are associated with subtle changes in cognitive function or affect. Methods Seventy SCD patients and 65 healthy controls were recruited. Demographic data, comprehensive neuropsychology assessments, and resting-state fMRI data were collected. The bilateral anterior and posterior hippocampi were selected as seeds to investigate the static and dynamic functional connectivity alterations in SCD. Results Compared to healthy controls, subjects with SCD exhibited: (1) decreased sFC between the left caudal hippocampus and left precuneus; (2) decreased dFC variability between the bilateral caudal hippocampus and precuneus; (3) increased dFC variability between the bilateral rostral hippocampus and caudate nucleus; and (4) increased dFC variability between the left rostral hippocampus and left olfactory cortex. Additionally, the attention scores were positively correlated with dFC variability between the left posterior hippocampus and left precuneus, and the dFC variability between the bilateral anterior hippocampus and caudate nucleus was positively correlated with depression scores and negatively correlated with global cognition scores. Conclusion SCD individuals exhibited abnormal sFC and dFC in the anterior-posterior hippocampus, and abnormal dFC was more widespread than abnormal sFC. A combination of sFC and dFC provides a new perspective for exploring the brain pathophysiological mechanisms in SCD and offers potential neuroimaging biomarkers for the early diagnosis and intervention of AD.

AIM: To investigate the suitability of a modified Hughes procedure, which consists of conjunctival flap with auricular cartilage grafting in reconstructing large full thickness upper and lower eyelid defect. METHODS: Patients with full thickness eyelid carcinoma involving more than 50% margin length who underwent surgical resection were retrospectively reviewed in the study. The defects were reconstructed using conjunctival flap with auricular cartilage grafting, covered with myocutaneous flap above. Followed-up time ranged from 12 to 24mo. Outcomes were classified as “good”, “fair”, and “poor” by evaluating the margin appearance, eyelid appearance, and complications. RESULTS: A total of 42 patients were enrolled in the study (26 males, 16 females, mean age, 68.6±7.7y, range: 53 to 82y). The mean defect widths measured 23.2±2.9 mm (range, 17 to 28 mm). The mean posterior lamellar defect height was 5.5±1.3 mm (4 to 8 mm). Thirty-seven patients had a “good” outcome (88.1%), 5 patients had a “fair” outcome (11.9%), and no one had a “poor” outcome. CONCLUSION: Conjunctival flap with auricular cartilage grafting and myocutaneous flap grafting is an effective procedure in reconstructing large full thickness upper and lower eyelid defect. It can not only achieve satisfied reconstruction, but also preserve intact tarsal plate of the opposite eyelid, avoiding retraction or entropion.

BACKGROUND Moebius syndrome (MBS) is a nonprogressive and rare congenital neuromuscular disorder involving the facial nerve and abductor nerve; it mainly manifests as facial paralysis and eye strabismus paralytic symptoms. Tissues in the oral cavity are also compromised, characterized by microstomia, micrognathia, tongue malformation, cleft lip, high arched palate or cleft palate, bifid uvula, and dental malocclusion. Therefore, dentistry plays a fundamental and crucial role in caring for these individuals. However, there is limited available data on MBS treatment, particularly regarding dental management. CASE SUMMARY This case report presents dental treatment of a 21-year-old man with MBS and discusses crucial interactions among oral complications of MBS. In this case, the patient was clinically characterized by congenital neuromuscular disorder, occlusal disorders, and tooth and gum problems. It is noteworthy that the patient presented early eruption of deciduous teeth 2 mo after birth, which has not been reported in other MBS cases and suggests a potentially new clinical manifestation of this syndrome. It is important to note that MBS cannot be cured, and oral manifestations of this syndrome can be managed by a multidisciplinary health care team that helps the patient maintain oral hygiene and dental health. After a series of oral treatments, no obvious poor oral hygiene, swollen gums, or abnormal imaging results were observed after 2 years of follow-up. CONCLUSION This case addressed the oral clinical manifestations of MBS and difficulties experienced during dental management, and suggested early tooth eruption as a potentially new clinical manifestation of this syndrome. Knowledge of the loop-mediated association among oral complications of this syndrome is essential to perfecting treatments.

In this study, a g-C3N4-Cu2O-TiO2 photocatalyst with a novel three-dimensional ordered macroporous (3DOM) structure was successfully prepared using a sacrificial template strategy and a photodeposition method. The influence of the special porous structure with cross pore channels on the photocatalytic properties of the as-prepared sample was studied in detail. Compared with the original photocatalyst (TiO2 with 3 wt% Pt), g-C3N4-Cu2O-TiO2 exhibited a higher specific surface area and more active sites, thus accelerating the separation efficiency of the photogenerated electron-hole pair. Consequently, the as-prepared photocatalyst showed good photocatalytic performance, reaching a maximum hydrogen production rate of 12,108 µmol g−1 h−1 and approximately five times higher than that of the pristine comparison sample. The enhanced photoactivity of the g-C3N4-Cu2O-TiO2 heterojunction can be ascribed to its double p-n heterojunction and robust porous structure, where the photodeposited Cu2O plays a synergistic catalytic role in the photocatalytic process and the outer clad g-C3N4 layer prevents Cu2O oxidation. Additionally, the possible photocatalytic mechanism was briefly discussed based on the experimental results. This work identifies viable pathways for developing low-cost heterojunction photocatalysts with highly efficient photocatalytic activity toward improved solar energy conversion.

A general synthesis of 4-hydroxylcarbazoles by domino vinylogous conjugate addition/cyclization/elimination/aromatization of easily prepared 3-nitroindoles with alkylidene azlactones under mild and transition-metal-free conditions has been developed. This method was also applicable to other nitrosubstituted benzofused heterocycles such as 3-nitrobenzothiophene, 2-nitrobenzothiophene, and 2-nitrobenzofuran. The valuable tetracyclic carbazole derivatives, such as 6H-oxazolo[4,5-c]carbazole and 3,6-dihydro-2H-oxazolo[4,5-c]carbazol-2-one, were readily prepared from the product, demonstrating synthetic utility of this method.

This study aimed to develop a vaccine that targets mutation-derived neoantigen in Chinese non-small-cell lung cancer (NSCLC).A cohort of 1862 Chinese NSCLC patients who underwent targeted sequencing with a 1021-gene panel was investigated. HLA typing was done using OptiType v1.0 and neoantigens were predicted by netMHCpan v4.0. HLA LOH was inferred using the lohhla algorithm and TMB were quantified by counting the total number of non-synonymous ones based on our panel data. CIBERSORT was utilized to estimate the TME in differentHLA-A*11:01(42.59%) was the top one allele and HLA-A*33:03(12.94%) ranked 12th.Our study identified that