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Objective: The objectives were to demonstrate that the nitrogen and energy in pig urine supplemented with hydrochloric acid (HCl) are not volatilized and to determine the minimum amount of HCl required for nitrogen preservation from pig urine.Methods: In Exp. 1, urine samples of 3.0 L each with 5 different nitrogen concentrations were divided into 2 groups: 1.5 L of urine added with i) 100 mL of distilled water or ii) 100 mL of 6 N HCl. The urine in open plastic containers was placed on a laboratory table at room temperature for 10 d. The weight, nitrogen concentration, and gross energy concentration of the urine samples were determined every 2 d. In Exp. 2, three urine samples with different nitrogen concentrations were added with different amounts of 6 N HCl to obtain varying pH values. All urine samples were placed on a laboratory table for 5 d followed by nitrogen analysis.Results: Nitrogen amounts in urine supplemented with distilled water decreased linearly with time, whereas those supplemented with 6 N HCl remained constant. Based on the linear broken-line analysis, nitrogen was not volatilized at a pH below 5.12 (standard error = 0.71 and pN HCl to preserve nitrogen in pig urine was developed: additional 6 N HCl (mL) to 100 mL of urine = 3.83×nitrogen in urine (g/100 mL)+0.71 with R2 = 0.96 and pN HCl should be added to the urine collection container.Conclusion: Nitrogen in pig urine is not volatilized at a pH below 5.12 at room temperature and the amount of 6 N HCl required for nitrogen preservation may be up to 240 mL per day for a 110-kg pig depending on urinary nitrogen excretion.

Objective: The objective was to test additivity of digestible energy and nutrient concentrations in the hatchery byproduct mixture fed to nursery pigs.Methods: In the previous studies, energy, phosphorus, calcium, and amino acid digestibility of infertile eggs, unhatched eggs, culled chicks, and a mixture of 3 hatchery byproduct ingredients was determined in nursery pigs (initial body weight = 9.4 to 14.2 kg). An additivity test was conducted using these determined values.Results: No difference was observed between determined and predicted metabolizable energy values in the mixture (3,998 and 3,990 kcal/kg as-is basis, respectively). Measured standardized total tract digestible phosphorus in the mixture was less than the predicted value (4.5 vs 5.3 g/kg as-is basis, respectively; p

Cinnamon is a natural spice with a wide range of pharmacological functions, including anti-microbial, antioxidant, and anti-tumor activities. The aim of this study is to investigate the effects of cinnamaldehyde-rich cinnamon extract (CRCE) on the colorectal cancer cell lines HCT 116 and HT-29. The gas chromatography mass spectrometry analysis of a lipophilic extract of cinnamon revealed the dominance of trans-cinnamaldehyde. Cells treated with CRCE (10–60 µg/mL) showed significantly decreased cell viability in a time- and dose-dependent manner. We also observed that cell proliferation and migration capacity were inhibited in CRCE-treated cells. In addition, a remarkable increase in the number of sub-G1-phase cells was observed with arrest at the G2 phase by CRCE treatment. CRCE also induced mitochondrial stress, and finally, CRCE treatment resulted in activation of apoptotic proteins Caspase-3, -9, and PARP and decreased levels of mu-2-related death-inducing gene protein expression with BH3-interacting domain death agonist (BID) activation.

Background: Alternative feed ingredients are widely used in swine diets to lower feed costs, but these ingredients contain a large quantity of non-starch polysaccharides. Supplemental xylanase is known to break down non-starch polysaccharides. However, the effects of exogenous xylanase from Bacillus subtilis on various feed ingredients have rarely been compared. Objective: To evaluate the effects of supplemental xylanase on in vitro disappearance of dry matter (DM) in various feed ingredients for pigs. Methods: Nine feed ingredients were used to measure in vitro ileal disappearance and in vitro total tract disappearance of DM. Each ground ingredient was supplemented with either supplemental xylanase (9,000 U/g) or cornstarch at 1.0%. Results: Supplemental xylanase increased in vitro ileal disappearance of DM in wheat, barley, wheat flour, and wheat bran (p

Objective: The objectives were to develop prediction equations for digestible energy (DE) and metabolizable energy (ME) of feed ingredients and diets for pigs based on chemical composition and to evaluate the accuracy of the equations using in vivo data.Methods: A total of 734 data points from 81 experiments were employed to develop prediction equations for DE and ME in feed ingredients and diets. The CORR procedure of SAS was used to determine correlation coefficients between chemical components and energy concentrations and the REG procedure was used to generate prediction equations. Developed equations were tested for the accuracy according to the regression analysis using in vivo data.Results: The DE and ME in feed ingredients and diets were most negatively correlated with acid detergent fiber or neutral detergent fiber (NDF; r = –0.46 to r = –0.67; pin vivo data well as follows: DE = 728+0.76×gross energy (GE)–25.18×NDF (R2 = 0.64); ME = 965+0.66×GE–24.62×NDF (R2 = 0.60); ME = 1,133+0.65×GE–29.05×ash–23.17×NDF (R2 = 0.67).Conclusion: In conclusion, the equations suggested in the current study would predict energy concentration in feed ingredients and diets.

Objective: The objective of this study was to investigate the influence of dietary lead (Pb) supplementation and feeding period on growth performance, organ weight, and Pb accumulation in pigs.Methods: In a 56-day feeding experiment, a total of 48 barrows with initial body weight 10.4±0.6 kg were allotted to 2 dietary treatments (0 and 200 mg/kg of supplemental Pb) in a completely randomized design with 6 replicates. Body weight and feed intake were recorded to calculate growth performance. At the end of each 14 day-period (on days 14, 28, 42, and 56), an animal was randomly selected from each pen and slaughtered to collect blood samples, hair samples, left 5th rib, heart, liver, kidneys, lungs, and longissimus dorsi muscle samples.Results: Average daily gain and average daily feed intake were reduced (p

The objective of this study was to develop equations for estimating ileal digestible crude protein (CP) and metabolizable energy (ME) contents of meat meal (MM) and meat and bone meal (MBM) as feed ingredients for pigs based on in vitro assays. Test ingredients were 4 sources of MM and 3 sources of MBM. Ash and CP contents of the ingredients ranged from 3.8% to 33.1% and 46.8% to 82.9% (as-is basis), respectively. In vitro ileal disappearance (IVID) of CP was determined and ileal digestible CP content was calculated by multiplying CP content by IVID of CP. In vitro total tract disappearance (IVTTD) of dry matter (DM) was determined and ME was calculated using gross energy, CP contents, and IVTTD of DM. The IVID of CP and IVTTD of DM ranged from 77.2% to 88.7% and from 82.7% to 92.4%, respectively. Calculated ileal digestible CP and ME contents ranged from 37.8% to 73.5% DM and 2,405 to 3,905 kcal/kg DM, respectively. Ash contents were negatively correlated (P

Objective: The objective was to determine standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in cereal grains and various co-products fed to growing pigs.Methods: Ten feed ingredients tested were barley (9.3% CP), lupin kernels (31.1% CP), and wheat (11.3% CP) as cereal grains, and 2 sources of corn gluten feed produced in China (21.6% CP) and Korea (24.6% CP), corn gluten meal (65.3% CP), lupin hulls (11.6% CP), rice bran (14.5% CP), soybean meal (44.8% CP), and wheat bran (15.4% CP) as co-products. Ten experimental diets were formulated to contain each ingredient as a sole source of N and an N-free diet was used to correct basal endogenous losses of CP and AA. All diets also contained 0.5% Cr2O3 as an indigestible index. A replicated 11×6 incomplete Latin square design with 11 dietary treatments, 6 periods, and 22 animals was employed. Twenty-two barrows with an initial body weight of 64.6±4.9 kg were equipped with a T-cannula in the distal ileum. An experimental period consisted of a 4-d adaptation period and a 2-d collection period.Results: The SID of CP in the barley, lupin kernels, wheat, 2 sources of corn gluten feed, corn gluten meal, lupin hulls, rice bran, soybean meal, and wheat bran were 84.7%, 90.5%, 90.4%, 77.4%, 74.6%, 89.5%, 90.4%, 74.4%, 86.9%, and 63.4% (standard error of the mean [SEM] = 5.3, p = 0.006), respectively. The respective SID values of Lys were 75.5%, 88.4%, 83.9%, 74.7%, 62.4%, 80.3%, 83.9%, 78.5%, 88.0%, and 71.2% (SEM = 3.3, p

Background: Soybean milk by-product (SMBP) is a potential alternative feed ingredient in swine diets due to its high protein content. However, information on energy and nutritional values of SMBP used as swine feed ingredient is limited. Objective: To estimate energy values and protein digestibility of SMBP in pigs based on in vitro assays. Methods: Four SMBP samples were obtained from 3 soybean milk-producing facilities. In vitro total tract disappearance (IVTTD) and in vitro ileal disappearance (IVID) of dry matter (DM) in the SMBP samples were determined. In vitro ileal disappearance of crude protein was determined by analyzing crude protein content in undigested residues after determining IVID of DM. Digestible and metabolizable energy of SMBP were estimated using gross energy, IVTTD of DM, and prediction equations. Results: Sample 4 had greater IVTTD of DM than that of sample 3 (97.7 vs. 94.4%, p

The objective was to determine the effects of drying and blanching methods on the nutrient utilization of black soldier fly larva (BSFL; Hermetia illucens) meal by pigs using in vitro assays. Two-step and three-step in vitro assays were employed to simulate the gastrointestinal tract of pigs. Four BSFL meals were prepared using the following pretreatment methods: (1) microwave drying at 80 °C for 32 min, (2) hot-air drying at 60 °C for 17 h, (3) blanching for 5 min in boiling water and hot-air drying at 60 °C for 17 h, and (4) 2% citric acid solution blanching for 5 min in boiling solution and hot-air drying at 60 °C for 17 h. After the drying process, each BSFL was defatted and ground to obtain BSFL meals. The nitrogen (N) concentration in the test ingredients ranged from 8.5 to 9.4%, and the ether extract ranged from 6.9 to 11.5% on an as-is basis. The amino acid (AA) concentration in the BSFL meals ranged from 2.80 to 3.24% for Lys and 0.71 to 0.89% for Met on an as-is basis. Hot-air-dried BSFL meal had a greater in vitro ileal disappearance (IVID) of N compared with microwave-dried BSFL meal (p < 0.05). However, blanched BSFL meals in water or 2% citric acid solution before hot-air drying had a lower (p < 0.05) IVID of N compared with microwave-dried or hot-air-dried BSFL meal. Blanched BSFL meals in water or 2% citric acid solution before hot-air drying showed a lower (p < 0.05) in vitro total tract disappearance of dry matter and organic matter compared with microwave-dried or hot-air-dried BSFL meal. Microwave-dried BSFL meal had a lower (p < 0.05) IVID of indispensable AA, except for His, Lys, Met, and Phe, compared with hot-air-dried BSFL meals. However, blanched BSFL meals in water or 2% citric acid solution before hot-air drying showed a lower (p < 0.05) IVID of indispensable AA compared with microwave-dried or hot-air-dried BSFL meal. In conclusion, hot-air-dried BSFL meal presented greater nutrient utilization compared with microwave-dried BSFL meal for pigs. However, blanching in water or citric acid solution negatively affected the nutrient digestibility of BSFL meal based on in vitro assays.

The objectives were to determine the effects of graded inclusion rates of wheat bran (WB) on apparent ileal (AID), apparent total tract (ATTD), and hindgut digestibility of nutrients and tested the influence of ileal digesta collection on proceeding fecal nutrient digestibility in pigs. Six barrows with an initial mean body weight of 70.7 ± 5.7 kg fitted with an ileal T-cannula were used. The animals were assigned to a replicated 3 × 3 Latin square design with three diets and three periods. A basal diet was based mainly on wheat, soybean meal, and cornstarch. Two additional diets were formulated to contain 20 or 40% of WB at the expense of cornstarch. Each experimental period consisted of a seven-day adaptation period and a four-day collection period. After the adaptation period, fecal samples were collected on day 8, and ileal digesta were collected on days 9 and 10. Another set of fecal samples was collected on day 11 to determine the influence of ileal digesta collection on proceeding total tract nutrient digestibility. The AID of energy, dry matter (DM), organic matter (OM), crude protein, and phosphorus linearly decreased (p < 0.05) with an increasing inclusion rate of WB from 0 to 40%. The ATTD of energy, DM, OM, crude protein, ether extract, and phosphorus linearly decreased (p < 0.01) as the inclusion rate of WB increased. Hindgut digestibility of DM, OM, and ether extract linearly increased (p < 0.05) with an increasing inclusion rate of WB. The ATTD of GE and most nutrients did not differ between the two fecal collection periods of before and after ileal digesta collection. Taken together, the inclusion of a fiber-rich ingredient reduced ileal and fecal digestibility of nutrients but increased hindgut digestibility of some nutrients, and total tract digestibility of nutrients did not differ whether the fecal samples were collected before or after two days of ileal digesta collection in pigs.

The objectives were to determine the digestible energy and standardized ileal digestibility of amino acids (AA; Exp. 1) and to determine growth performance (Exp. 2) of two sources of dietary spray-dried plasma protein (SDPP) in nursery pigs. In Exp. 1, twelve nursery barrows (9.8 ± 0.9 kg) were assigned to a quadruplicated 3 × 2 Latin square design with three diets and two periods. Each period consisted of 5 d of adaptation, 2 d of fecal sampling, and 2 d of ileal collection. A basal diet was composed of corn, soybean meal, whey, and sucrose as the sole energy and AA sources. Experimental diets were prepared by replacing 15% of the energy and AA sources in the basal diet with SDPP 1 (manufactured in the United States; 78.2% crude protein and 4,862 kcal gross energy/kg as-is) or SDPP 2 (manufactured in Korea; 74.3% crude protein and 4,636 kcal gross energy/kg as-is). Spray-dried plasma protein 1 had greater digestible energy (P0.05), but less (P0.05) standardized ileal digestibility of Lys, Met, Trp, and Thr compared with SDPP 2. In Exp. 2, eighty-four nursery pigs (7.9 ± 0.7 kg) were allotted to three dietary treatments in a randomized complete block design with seven replicate pens and four pigs per pen. Three corn-soybean meal-whey-based diets contained fish meal (6% and 3.5% for days 0 to 14 and 14 to 28, respectively), SDPP 1 (4.5% and 2.7%), or SDPP 2 (5.0% and 3.0%) to maintain same energy and nutrient concentrations. During days 0 to 14 and overall period, pigs fed the diets containing SDPP gained more weight (P0.05) than those fed the fish meal diet with no difference between two SDPP sources. In conclusion, SDPP 1 contains greater digestible energy but less AA digestibility compared with SDPP 2. Growth-promoting effects of both SDPP sources in nursery diets have been clearly demonstrated in this work.Dietary spray-dried plasma protein (SDPP) is widely used in nursery pig diet due to its high nutrient contents and growth-promoting effects. In the present work, two sources of SDPP were evaluated for energy and amino acid (AA) digestibility and growth performance in nursery pigs. The SDPP 1 produced in the United States contained 78.2% crude protein and 4,862 kcal gross energy/kg and SDPP 2 produced in Korea contained 74.3% crude protein and 4,636 kcal gross energy/kg. Spray-dried plasma protein 1 had a greater digestible energy concentration, but less AA digestibility compared with SDPP 2. Pigs fed the diets containing SDPP consumed more feed and grew faster than those fed the fish meal diet with no difference between the two sources of SDPP. Taken together, SDPP 1 contains greater digestible energy but less AA digestibility compared with SDPP 2. Growth-promoting effects of two sources of SDPP on nursery pigs are greater than fish meal with no difference between the two sources of SDPP.

The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) concentrations in high-fiber ingredients fed to growing pigs. Twelve barrows with an initial body weight of 57.5 kg (SD = 5.7) were individually housed in metabolism crates. A replicated 6 × 3 incomplete Latin square design with 12 animals, 6 experimental diets and 3 periods was employed. A basal diet was composed of 75.0% corn and 22.7% soybean meal (SBM) as the sole energy sources. Four experimental diets were prepared by replacing 40% of corn and SBM with soybean hulls (SH), corn gluten feed (CGF), wheat bran (WB), or rice bran (RB). An additional diet was prepared by replacing 10% of corn and SBM with cashew nut hulls (CNH). Each period consisted of a 4-d adaptation period and a 4-d collection period, and the marker-to-marker procedure was used for total collection of feces and urine. The DE and ME values in RB (3,969 and 3,936 kcal/kg DM) were greater (P < 0.05) than those in CGF (2,654 and 2,520 kcal/kg DM) and SH (2,492 and 2,541 kcal/kg DM) and the energy values in WB (3,162 and 3,118 kcal/kg DM) were not different from those in RB, CGF, or SH. The DE and ME values in CNH (350 and 572 kcal/kg DM) were less (P < 0.05) than those in all other test ingredients. In conclusion, energy concentrations in RB were greatest among the high-fiber test ingredients, whereas CNH had the lowest values.

Two experiments were conducted to determine In Vitro Dry Matter Disappearance (IVDMD) of Brewers Dried Grains (BDG) and corn Distillers Dried Grains with Solubles (DDGS) and to investigate effects of supplemental β-glucanase on the IVDMD in barley, BDG and corn DDGS for pigs. In experiment 1, the IVDMD in corn DDGS and two BDG sources produced from Cameroon and Republic of Korea were determined. A three-step IVDMD procedure was employed to simulate digestion of nutrients in the test ingredients by pigs. The digestion 3 steps were for the stomach, the small intestine and the large intestine of pigs. All digestion procedures were conducted in triplicate. The IVDMD in corn DDGS and two BDG sources from Cameroon and Korea was 62.2, 49.2 and 41.0% (±0.6; p

Two experiments were conducted to determine the digestibility of energy and Crude Protein (CP) in Korean Rice Wine Residues (RWR) using in vitro and in vivo approaches. In the in vitro experiment, 2-step and 3-step in vitro disappearance procedures were employed to determine in vitro Ileal Disappearance (IVID) and in vitro Total Tract Disappearance (IVTTD) of nutrients, respectively, in corn, a Soybean Meal-Wheat Mixture (SBMW), RWR and the experimental diets used in the in vivo experiment. In the in vivo experiment, 3 Jeju Island native pigs fitted with a T-cannula in the distal ileum with an initial mean body weight of 74.8±5.4 kg were individually housed in pens equipped with a feeder and a nipple drinker. A replicated 3×3 Latin square design was employed with 3 dietary treatments, 6 periods and 3 animals. Three experimental diets based on the corn and SBMW with a constant ratio (corn:SBMW = 3.06:1) were formulated to contain 0, 15 or 30% of RWR. All diets contained 0.5% chromic oxide as an indigestible index. Korean RWR had greater IVID of Dry Matter (DM) than corn and SBMW (p

The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P < 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P < 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.

Sufficient supply of dietary phosphorus (P) is critical for maximizing muscle and bone growth of pigs. The objective was to compare standardized total tract digestible (STTD) P requirements of growing pigs determined in the experiments and those estimated using modeling approach. Fifty-two experiments in the literature that empirically determined P requirements of pigs were employed. The basis for the determination of P requirements were growth performance (average daily gain or gain to feed ratio; n = 34) and bone development (n = 23). Based on the STTD P in the feed ingredients provided in NRC (2012) and ingredient composition used in the experiments, P requirements presented as total P, available P, and true total tract digestible P were converted to STTD P requirements. To obtain STTD P requirements (g/d) suggested by NRC (2012) for grow-finishing pigs (body weight > 20 kg), mean body weight and sex indicated in the experiments were input into the NRC model. The statistical model for comparing the empirical data with the NRC requirements included the basis for determining P requirements in the experiments as a fixed variable and body weight as a random variable. To quantify the deviations between NRC STTD P requirement estimates and empirically determined STTD P requirements, mean percentage difference was calculated. The STTD P requirements empirically determined based on growth performance were greater than the NRC STTD P requirement estimates (5.89 vs. 4.61 g/d, SEM = 0.27; P < 0.001). Moreover, the bone development-based STTD P requirements were greater than the NRC STTD P requirement estimates (6.63 vs. 4.46 g/d, SEM = 0.42; P < 0.001). In conclusion, the standardized total tract digestible P requirements suggested by the NRC are less than the experimentally determined requirements.

The objectives of the present work were to assess the accuracy of previously published equations for predicting effects of deoxynivalenol (DON) on the growth performance changes of pigs and to update equations based on recently published data. A total of 59 data were employed for the validation of previously published equations. These data were used to update the equations. The REG and CORR procedures of SAS were used. In the present validation test, a linear bias was significant (p < 0.05), indicating that prediction errors were not consistent across the data ranges. The intercept for ΔFI (−7.75 ± 1.19, p < 0.01) representing a mean bias was less than 0, indicating that the predicted mean of ΔFI was greater than the measured mean of ΔFI. Dietary DON concentrations had negative correlations with ΔWG (r = −0.79; p < 0.01) and ΔFI (r = −0.71; p < 0.01). Updated prediction equations were: ΔWG = −5.93 × DON with r2 = 0.77 and ΔFI = −4.42 × DON with r2 = 0.68. In conclusion, the novel equations developed in this study might accurately predict effects of dietary DON on the performance changes of pigs.

The objectives of the present study were to determine the influence of thermal and non-thermal processing procedures on in vitro ileal disappearance (IVID) of dry matter (DM) and crude protein (CP) in chicken meat as dog foods using 2-step in vitro assays. In thermal processing experiments, IVID of DM and CP in chicken meat thermally processed at 70, 90, and 121 °C, respectively, with increasing processing time was determined. For non-thermal processing experiments, IVID of DM and CP in chicken meat processed by high-pressure, ultraviolet-light emitting diode (UV-LED), electron-beam, and gamma-ray was determined. Thermal processing of chicken meat at 70, 90, and 121 °C resulted in decreased IVID of CP (p &lt, 0.05) as heating time increased. In non-thermal processing experiment, IVID of CP in chicken meat was not affected by high-pressure processing or UV-LED radiation. In vitro ileal disappearance of CP in electron-beam- or gamma-ray-irradiated chicken meat was not affected by the irradiation intensity. Taken together, ileal protein digestibility of chicken meat for dogs is decreased by thermal processing, but is minimally affected by non-thermal processing methods.

Objective: The objectives of the present study were to determine digestible energy (DE), metabolizable energy (ME), and standardized total tract digestibility (STTD) of phosphorus (P) in meat meal (MM) and to compare these values with those in fish meal (FM), and soybean meal (SBM) fed to pigs.Methods: Two experiments were conducted to determine energy concentrations and STTD of P in MM, FM, and SBM fed to growing pigs. In Exp. 1, DE and ME in the 3 test ingredients were measured using 24 barrows with initial body weight (BW) of 77.7±8.3 kg. A corn-based diet and 3 diets containing corn and 22% to 30% of each test ingredient were prepared. In Exp. 2, the STTD of P in the 3 test ingredients was measured using 24 barrows (90.9±6.6 kg BW). Three diets were formulated to contain each test ingredient as the sole source of P.Results: In Exp. 1, the DE and ME values in MM (3,310 and 2,856 kcal/kg dry matter [DM]) were less (p

Amino acid (AA)-to-crude protein (CP) ratios in soybean meal (SBM) may be different for different sources of SBM depending on the presence of additional hulls. Therefore, this study was conducted to develop novel two-slope equations to predict the concentrations of AAs in SBM using CP as an independent variable. Regression analyses were performed with each AA in SBM as the dependent variable and the CP as the independent variable. Among all AAs, the predicted Lys in SBM (% dry matter (DM)) was: Lys = 3.19 − 0.026 × (51.88 − CP) where CP < 51.88% DM and Lys = 3.19 + 0.072 × (CP − 51.88) where CP > 51.88% DM with R2 = 0.51 and p < 0.001. In conclusion, the novel equations provided reasonable estimates of the AA concentrations from different ranges of CP in SBM.

Emulsion gel has been used to replace animal fats in meat products. Konjac is a widely used gelling agent; however, its low emulsion stability limits its use in meat products. This study aimed to examine the quality characteristics of β-cyclodextrin (CD)-supplemented konjac-based emulsion gel (KEG) (CD-KEG) and its application as a fat substitute in emulsion-type sausages. The supplementation of CD increased hydrogen bonds and hydrophobic interactions with konjac and oil in the gels, respectively. Additionally, CD increased the structural complexity and strength of KEG. Since adding more than 6% of CD to KEG did not increase the gel strength, 6% CD-added KEG was adopted to substitute for pork backfat in manufacturing low-fat emulsion-type sausages. The following formulations of the sausages were prepared: pork backfat 20% (PF20); pork backfat 10% + KEG 10% (KEG10); KEG 20% (KEG20); pork backfat 10% + CD-KEG 10% (CD-KEG10); CD-KEG 20% (CD-KEG20); and pork backfat 5% (PF5). The CD-KEG20 formulation exhibited higher viscosity and viscoelasticity than KEG20, which suggested that CD improves the rheological properties and the thermal stability of meat batter. Additionally, CD-KEG20 showed similar emulsion stability, cooking yield and texture parameters compared with PF20. Therefore, 6% CD-added KEG is a suitable fat substitute for preparing low-fat emulsion-type sausages.

The objectives were to demonstrate that nitrogen volatilization from pig urine can be inhibited by the addition of acids and to determine a minimum amount of HCl for nitrogen preservation in pig urine. In experiment 1, five urine samples were collected and had nitrogen concentrations of 0.29% to 0.68%. Each sample was divided into 2 groups that were supplemented with 100 mL of distilled water and 100 mL of 6 N HCl, respectively. The samples were placed in open containers at room temperature for 10 d. The nitrogen concentration was determined every 2 d. The amount of nitrogen in urine supplemented with distilled water decreased linearly (P < 0.001) with time, whereas that supplemented with 6 N HCl remained constant. In experiment 2, three urine samples with different nitrogen concentration (0.12%, 0.53%, and 0.94%) were added with different amounts of 6 N HCl used to make varying acidity (pH = 0.6, 1.1, 2.2, 4.7, 7.1, and 9.3). All urine samples were placed at a room temperature for 5 d followed by nitrogen analysis. Based on a linear broken-line analysis, nitrogen was not volatilized at a pH less than 5.12 (SE = 0.71), whereas nitrogen was volatilized at a pH greater than 5.12 (r2 = 0.98). An equation was developed for the minimum amount of 6 N HCl in urine to avoid nitrogen volatilization: 6 N HCl in urine (g/L) = 0.11 × nitrogen in urine (g/L) + 0.2 with r2 = 0.93 and P < 0.01. Assuming that up to 200 gram of nitrogen is excreted for 5 d, the addition of 100 mL of 6 N HCl to the urine collection container can inhibit nitrogen volatilization. Taken together, a minimum of 100 mL of 6 N HCl is recommended for 5 day-total collection of pig urine.

The objectives were to determine standardized ileal digestibility (SID) of amino acids (AA) in hatchery byproducts fed to nursery pigs and to test the additivity of ileal digestible AA concentrations in hatchery byproducts. Ten barrows (initial body weight = 11.3 ± 1.3 kg) surgically fitted with T-cannulas at the distal ileum were individually housed in pens. Four diets were prepared to contain 30% of infertile eggs, unhatched eggs, culled chicks, or a mixture (20% dried infertile eggs + 20% dried unhatched eggs + 60% dried culled chicks) as the sole source of nitrogen. A nitrogen-free diet was additionally prepared to measure basal endogenous losses of AA. All experimental diet contained 0.5% chromic oxide as an indigestible index. Ten pigs were assigned to a replicated 5 × 4 Latin square design with 5 diets and 4 periods. Each period consisted of a 5-d adaptation period and a 2-d collection period. The hypothesis was that the determined digestible AA concentrations of the mixture are not different from the calculated values based on digestible AA in the individual ingredients. A t-test was used to determine the additivity of digestible AA concentrations in individual ingredients. Infertile eggs had greater (P < 0.05) SID of all indispensable AA except for lysine and methionine compared with culled chicks. The SID of all indispensable AA except for tryptophan was not different between culled chicks and the mixture. The calculated apparent and standardized ileal digestible AA in the mixture were not different from the determined values, but calculated ileal digestible tryptophan in the mixture was less (P < 0.05) than the measured value. In conclusion, ileal digestibility of most indispensable AA in infertile eggs is greater than that in culled chicks and ileal digestible AA values are additive in the mixture except for tryptophan.

The aim of the present study was to determine the effects of supplemental protease on in vitro disappearance of dry matter (DM) and crude protein (CP) in feed ingredients for pigs. The test ingredients were three sources of feather meal (FM 1, FM 2, and FM 3), meat and bone meal, soybean meal, corn dried distillers grains with solubles, copra meal (CM), palm kernel meal, wheat, and corn. In experiment 1, in vitro ileal disappearance (IVID) of DM and CP were determined to represent the digestion and absorption of the stomach and small intestine of pigs. In experiment 2, in vitro total tract disappearance (IVTTD) of DM was determined to represent the digestion and absorption of the stomach, small intestine, and large intestine of pigs. The control group had 99% of a test ingredient and 1% of wheat bran. Protease originating from Bacillus spp. was supplemented to the control diet at the expense of wheat bran to make a treatment group (100,000 U/kg of sample). Protease supplementation increased the IVID of DM in FM 1 (39.8 vs. 37.5%) and CM (45.0 vs. 42.2%). In the protease supplementation group, the IVID of CP also increased in FM 1 (48.0 vs. 43.8%) and CM (85.3 vs. 77.5%). The supplemental protease increased the IVTTD of DM in FM 1 (43.9 vs. 42.1%), FM 3 (55.6 vs. 52.7%), and meat and bone meal (85.9 vs. 84.3%). Therefore, nutrient utilization of FM and CM can be improved by supplemental protease originating from Bacillus spp. based on in vitro assays.

The objective of this editorial is to present standard guidelines in a peer review process for reviewers. The editorial contains acceptance of invitation, review process, decision for manuscripts, and confidentiality. In addition, this editorial also helps authors publication in Animal Bioscience. This editorial does not aim to provide immutable guidelines for reviewing, however, it can assist reviewers with appropriate and effective ways.

This experiment was conducted to growth curve models for leptin-deficient (ob/ob) and wild-type mice fed a meal-form High-Fat (HF) diet prepared in-house for over 120 weeks. Two hundred and twenty-four sets of Body Weight (BW) and age data were collected from ob/ob mice and 485 sets of data were collected from wild-type mice. All animals had free access to the HF diet (34.3% energy from fat) and water. Individual BW and survival rates of mice were measured. To develop growth curves of ob/ob mice and wild-type mice fed the HF diet, Gompertz and Logistic growth models were employed. The survival rates of ob/ob mice fed the HF diet were lower than those of wild-type mice. Models for estimating growth of ob/ob mice fed the HF diet were: BW, g = 61.36 × e-2.56×e^-0.13t in Gompertz model (Akaike’s Information Criterion, AIC = 1,694); and BW, g = 60.65 × (1 + 7.04 × e-0.18t)-1 in Logistic model (AIC = 1,719). Growth models for wild-type mice fed the HF diet were: BW, g = 28.94 × e-1.75×e^-0.13t in Gompertz model (AIC = 3,171); and BW, g = 28.23 × (1 + 4.85 × e-0.25t)-1 in Logistic model (AIC = 3,198). In conclusion, Gompertz models may be more appropriate to estimate the growth of ob/ob mouse fed the diet with high-fat concentration.

The objective of this study was to determine the efficacy of mycotoxin sequestering agents for aflatoxin B1 (AFB1), deoxynivalenol (DON), and zearalenone (ZEA) using an in vitro method. The twelve toxin sequestering agents tested were seven bentonite products (bentonite A, B, C, D, E, F, and G), two aluminosilicate products (aluminosilicate A and B), a heulandite product, an activated charcoal product, and a yeast cell wall product. A two-step in vitro procedure was employed to mimic the conditions of temperature, pH, and digestive enzymes in the stomach and small intestine of pigs. All mycotoxin sequestering agents tested were able to bind to AFB1 with a high efficacy (>92%). The DON sequestering rate of activated charcoal (99.1%) was greater (p < 0.05) than that of other products. The ZEA sequestering rate of bentonite F (97.0%), aluminosilicate A (99.6%), and activated charcoal (100.0%) was the greatest (p < 0.05) among the tested mycotoxin sequestering agents. Overall, most mycotoxin sequestering agents had the ability to bind to AFB1, but most products, except activated charcoal, failed to sequester DON and ZEA.

Sufficient supply of dietary phosphorus (P) is critical for maximizing muscle and bone growth of pigs. The objective was to compare standardized total tract digestible (STTD) P requirements of growing pigs determined in the experiments and those estimated using modeling approach. Fifty-two experiments in the literature that empirically determined P requirements of pigs were employed. The basis for the determination of P requirements were growth performance (average daily gain or gain to feed ratio; n = 34) and bone development (n = 23). Based on the STTD P in the feed ingredients provided in NRC (2012) and ingredient composition used in the experiments, P requirements presented as total P, available P, and true total tract digestible P were converted to STTD P requirements. To obtain STTD P requirements (g/d) suggested by NRC (2012) for grow-finishing pigs (body weight > 20 kg), mean body weight and sex indicated in the experiments were input into the NRC model. The statistical model for comparing the empirical data and the NRC requirements included the basis for determining P requirements in the experiments as a fixed variable and body weight as a random variable. To quantify the deviations between NRC STTD P requirement estimates and empirically determined STTD P requirements, mean percentage difference was calculated. The STTD P requirements empirically determined based on growth performance were greater than the NRC STTD P requirement estimates (5.89 vs. 4.61 g/d, SEM = 0.27; P < 0.001). Moreover, the bone development-based STTD P requirements were greater than the NRC STTD P requirement estimates (6.63 vs. 4.46 g/d, SEM = 0.42; P < 0.001). In conclusion, the standardized total tract digestible P requirements suggested by the NRC are less than the experimentally determined requirements.

The objective was to determine the influence of feed intake (FI) and fiber source on hindgut disappearance of energy and nutrients in pigs. Ten canulated barrows with an initial body weight (BW) of 38.3 ± 5.4 kg were allotted to a replicated 5 × 4 Latin square design with 5 treatments and 4 periods. A corn-soybean meal-based basal diet (BD) and 2 × 2 factorial treatment arrangement with 2 amounts of FI (9.0 and 4.5% × BW0.75) and 2 fiber sources of sugar beet pulp (SBP) and corn cob were used. Three diets were 1) a BD, 2) a diet replacing 30% of corn and soybean meal in BD with SBP as a source of soluble dietary fiber (SDF), and 3) a diet replacing 15% of corn and soybean meal in BD with corn cobs as a source of insoluble dietary fiber (IDF) to obtain a similar IDF concentration as in the SBP diet. Each period consisted of 7-d adaptation, 2-d fecal collection, and 2-d ileal collection. Apparent ileal digestibility (AID) of energy, dry matter (DM), organic matter (OM), and crude protein (CP) in SBP diet was less (P < 0.001) than that in corn cob diet, whereas AID of neutral detergent fiber in SBP were greater (P < 0.001) than in corn cob diet. Hindgut disappearance of energy, DM, OM, neutral detergent fiber, and acid detergent fiber in SBP diet was greater (P < 0.05) than that in corn cob diet. However, no interaction between FI and fiber source on the AID, apparent total tract digestibility, and hindgut disappearance of nutrients was observed. Overall, hindgut disappearance of nutrients in sugar beet pulp diet was greater than that in corn cob diet. However, the influence of feed intake or the interaction between feed intake and fiber source was not observed.

An experiment was conducted to determine the standardized total tract digestibility (STTD) of phosphorus (P) in five sources of inorganic phosphate fed to growing pigs, including dicalcium phosphate (DCP), monodicalcium phosphate (MDCP), monocalcium phosphate (MCP), tricalcium phosphate (TCP) and monosodium phosphate (MSP, reagent grade). Six barrows (42.4 ± 1.1 kg) individually housed in metabolism crates were allotted to a 6 × 6 Latin square design with six dietary treatments and six periods. Each experimental period consisted of a 4 day adaptation period and a 5 day collection period. The five experimental diets contained 0.24 to 0.34% of P from each inorganic phosphate as a sole source of P. A P-free diet was also prepared to estimate the basal endogenous loss of P. The STTD of P in MSP (94.9%) was not different from the STTD of P in MCP (93.0%), but was greater (P

One of the goals of swine diet formulation is minimizing nitrogen (N) excretion as well as maximizing N retention. This study was conducted to test the hypothesis that different requirement expressions of dietary amino acids (AA) may affect N balance in pigs. Twelve pigs with average body weight of 40.9 kg (standard deviation =3.3) were used in a quadruplicated 3×3 Latin square design. Three diets were formulated to meet AA requirement estimates based on total AA (Diet total ), apparent ileal digestible AA (Diet AID ), or standardized ileal digestible AA (Diet SID ) with constant amounts of corn, soybean meal, and corn germ meal but different crystalline AA contents. Total feces and urine were collected. Nitrogen balance was calculated based on the analyzed data of the diets, feces, and urine samples. Preplanned orthogonal contrasts were used to compare the least squares means among treatments: 1) Diet total vs. Diet AID and Diet SID and 2) Diet AID vs. Diet SID . Pigs fed Diet total had less retained N ( P =0.048) and retention coefficients ( P P =0.014) and total ( P =0.017) N excretion than Diet AID and Diet SID . Urinary N excretion tended to be greater ( P =0.074) for pigs fed Diet AID than Diet SID . In conclusion, formulating swine diets based on digestible AA rather than on total AA can reduce N excretion.

The objectives of this study were to assess the accuracy of previously published equations for predicting effects of deoxynivalenol (DON) on the growth performance of pigs and to update the equations based on the recently published data. A total of 55 data from 22 experiments published between 2013 and 2020 reporting the effects of DON on the performance of pigs were employed for the validation of the previously published equations and these data were used to update the equations. The REG procedure of SAS was used to validate and develop equations and the CORR procedure was used to determine correlation coefficients among variables. The validation of the previously published equations showed that the slope (0.33 ± 0.08, P < 0.05) representing the linear bias and intercept (-3.55 ± 1.60, P < 0.05) representing the mean bias of weight gain changes (ΔWG, %) were different from 0. The slope (0.55 ± 0.07, P < 0.05) and intercept (-6.94 ± 1.20, P < 0.05) of feed intake changes (ΔFI, %) were also different from 0. Dietary DON concentrations had a negative correlation with ΔWG (r = -0.80; P < 0.001) and ΔFI (r = -0.74; P < 0.001). Updated prediction equations for estimating ΔWG and ΔFI based on a total of 152 data from 41 experiments were: ΔWG = -5.76 × DON with r2 = 0.77 and ΔFI = -4.65 × DON with r2 = 0.71, respectively, where dietary DON concentrations ranged from 0 to 14.6 mg/kg. In conclusion, the novel equations developed based on the large number of observations including recent ones may accurately predict effects of dietary DON on the performance of pigs.

The objective was to investigate the influence of crude protein (CP) concentrations in the basal diet on the metabolizable energy (ME) of feed ingredients determined using the difference procedure in pigs. Twelve barrows (73.7 ± 5.5 kg body weight) individually housed in metabolism crates were used. A low-protein basal diet (LPBD, 7.4% CP) was composed of 97.5% corn as the sole energy source and a high-protein basal diet (HPBD, 14.3% CP) was composed of 78.0% corn and 19.6% soybean meal. Four additional diets were prepared by replacing 20% of the energy sources in the basal diets with full-fat soybean (FFSB, 37.7% CP) or soy protein isolate (SPI, 87.6% CP). The 6 experimental diets were fed to 12 pigs employing a replicated 6 × 4 incomplete Latin square design with 4 periods. Each period was consisted of 4 days of adaptation and 4 days of collection period and the marker-to-marker method was used for fecal collection. Urinary gross energy output was greater (243 vs. 176 kcal/d; P < 0.05) in the HPBD group compared with the LPBD group. While ME in the HPBD was greater (3,418 vs. 3,322 kcal/kg; P < 0.05) than in the LPBD, ME in FFSB diets (3,585 and 3,633 kcal/kg in the LPBD and HPBD group, respectively) and SPI diets (3,537 and 3,537 kcal/kg, respectively) were not different between the 2 basal diet groups. Metabolizable energy of the test ingredients was less (P < 0.05) in pigs fed the HPBD group (4,565 and 4,111 kcal/kg in FFSB and SPI, respectively) compared with pigs fed the LPBD group (4,756 and 4,517 kcal/kg in FFSB and SPI, respectively). In conclusion, as the protein in the basal diet increases, metabolizable energy in a test ingredient determined using the difference procedure decreases mainly due to greater urinary energy output in pigs.

The objectives were to determine effects of a hatchery byproduct mixture (HBM) on growth performance and to measure digestible energy concentrations in various HBM. In the growth performance experiment, 96 pigs (initial body weight = 9.6 &plusmn, 0.8 kg) were assigned to 4 dietary treatments in a randomized complete block design with 6 blocks. Each treatment consisted of 6 replicate pens with 4 pigs comprising 2 barrows and 2 gilts. Pigs were fed graded concentrations of HBM at 0%, 3.33%, 6.67%, and 10.00% for 14 days. In the energy digestibility experiment, 10 barrows (initial body weight = 11.5 &plusmn, 0.4 kg) were employed to determine digestible energy in HBM. A basal diet based on corn and soybean meal and 4 additional diets containing 25% of 4 different HBM were prepared. The marker-to-marker method was employed for total collection and the experimental design was a replicated 5 &times, 4 Latins square design. Growth performance was not compromised as the inclusion rate of HBM increased up to 10%. Digestible energy of HBM ranged from 2772 to 3887 kcal/kg as-is basis. In conclusion, HBM can be used in nursery pig diets and different energy values should be used for each HBM.

The objective was to validate the prediction equations for digestibility of gross energy and amino acids in commercial pigs using mini pigs. Five commercial crossbred barrows (Landrace × Yorkshire) with initial BW of 62.6 ± 6.1 kg and 5 mini pigs selected from Jeju Island native pigs (M-Pig; Cronex Co., Ltd., Hwaseong, Republic of Korea) with initial BW of 53.4 ± 7.4 kg were used. All pigs were fitted with a T-cannula at the distal ileum, and 5 animals of each breed were randomly allotted to a 5 × 10 Latin square design with 5 diets and 10 periods, respectively. A basal diet contained 98% corn and 4 experimental diets contained 20 or 40% rapeseed meal or wheat distillers dried grains with solubles at the expense of corn. Chromic oxide was included at 0.5% as an indigestible index. The accuracy of previously developed prediction equations for nutrient digestibility of commercial pigs was assessed by regressing measured values from commercial pigs minus predicted values from mini pigs on the predicted values centered to the mean. Comparison of measured and predicted apparent total tract digestibility of gross energy showed that the intercept (0.02 ± 0.81; P = 0.985) indicating mean bias and the slope (0.08 ± 0.15; P = 0.599) indicating linear bias were not different from 0. The equation for standardized ileal digestibility of lysine and arginine did not have mean bias or linear bias (P > 0.05). However, the equations for standardized ileal digestibility of other amino acids had mean bias or linear bias (P < 0.05). In conclusion, energy digestibility of commercial pigs may be predicted by previously developed prediction equations for commercial pigs using mini pigs.

The objective of this study was to determine the additivity of energy concentrations and phosphorus (P) and calcium (Ca) digestibility in hatchery byproducts. In our previous studies, digestible energy (DE), metabolizable energy (ME), and apparent total tract digestibility (ATTD) and standardized total tract digestibility (STTD) of P and Ca were determined using 10-kg barrows. Test ingredients were infertile eggs, unhatched eggs, culled chicks, and a mixture (20% infertile eggs, 20% unhatched eggs, and 60% culled chicks). It was hypothesized that determined nutritional values of the mixture is not different from predicted values calculated from nutritional values in each ingredient. A t-test was used to determine the additivity on nutritional values in the mixture. The predicted DE in the mixture was not different from the determined value (4,203 and 4,224 kcal/kg as-is; P = 0.890), and the predicted ME was not different from the determined value (3,990 and 3,998 kcal/kg as-is; P = 0.959). Predicted ATTD and STTD of P in the mixture (46.4 and 58.4%, respectively) were greater than determined values (35.5 and 47.4%, respectively; P < 0.05). However, predicted ATTD and STTD of Ca in the mixture (45.5 and 51.7%, respectively) were less than determined values (58.0 and 64.5%, respectively; P < 0.05). In conclusion, energy concentrations in a hatchery byproduct mixture could be predicted as a weighted mean of energy values in infertile eggs, unhatched eggs, and culled chicks, whereas phosphorus and calcium digestibility values in the mixture were not additive.

Two experiments were conducted to investigate the effects of phytase source and feed pelleting on digestibility of dry matter (DM), crude protein, organic matter (OM), and phosphorus (P) with different doses of phytase in pigs. In Exp. 1, ten barrows (31.4 ± 3.5 kg of BW) were individually housed in metabolic crates and allotted to a replicated 5 × 6 Latin square design with 5 diets and 6 periods. In Exp. 2, ten barrows (28.2 ± 3.2 kg of BW) were individually housed in metabolic crates and allotted to a replicated 5 × 5 Latin square design with 5 diets and 5 periods. Each period lasted 10 d consisted of a 5-d adaptation and a 5-d collection periods and the marker to marker method was used for fecal collection. In both experiments, a basal diet was prepared based on corn and soybean meal without phytase or inorganic P. Four additional diets were prepared in a 2 × 2 factorial treatment arrangement with 2 phytase sources (Buttiauxella spp. or Escherichia coli) and 2 feed forms (mash or pellet). Both experiments employed the same treatments but had different supplemental phytase doses (500 and 1,500 FTU/kg in Exp. 1 and 2, respectively). In Exp. 1, supplemental phytase increased standardized total tract digestibility of P (66.1 vs. 51.7%; P < 0.001) with no effects of phytase source or feed pelleting. Feed pelleting increased digestibility of DM, crude protein, and OM (P < 0.001). In Exp. 2, supplemental phytase increased standardized total tract digestibility of P (72.8 vs. 44.8%; P < 0.001) with no effects of phytase source or feed pelleting. Feed pelleting increased digestibility of DM and OM (P < 0.001). Taken together, supplemental phytase at 500 and 1,500 FTU/kg increases P digestibility regardless of phytase source or feed pelleting.

The objective was to determine standardized total tract digestibility (STTD) of calcium (Ca) in hatchery byproducts fed to nursery pigs. Ten barrows with mean body weight of 10.2 ± 0.7 kg were individually housed in metabolism crates. Four corn-based diets were formulated to contain 10% of infertile eggs (40% CP, 11.6% Ca, and 4,630 kcal/kg GE as-is), 10% of unhatched eggs (40% CP, 12.7% Ca, and 4,296 kcal/kg GE as-is), 25% of culled chicks (66% CP, 1.8% Ca, and 5,897 kcal/kg GE as-is), and 15% of a mixture of 3 ingredients aforementioned (56% CP, 6.2% Ca, and 5,342 kcal/kg GE as-is), respectively. A calcium-free diet was also prepared to measure basal endogenous losses of Ca. Ten animals were assigned to a replicated 5 × 4 Latin square design with 5 diets, and 4 periods. Each period consisted of a 5-d adaptation period and 5-d collection period. The marker to marker method was employed for total feces collection. The STTD of Ca in culled chicks (75.6%) was greater than that in the mixture (64.5%; P < 0.05). The ATTD and STTD of Ca in unhatched eggs (38.3 and 43.4%, respectively) were less than those in infertile eggs (48.5 and 53.3%, respectively; P < 0.05). Basal endogenous losses of Ca were determined at 659 mg/kg of dry matter intake. In conclusion, culled chicks had the greatest standardized total tract digestibility of calcium among test ingredients and standardized total tract digestible calcium in infertile eggs, unhatched eggs, culled chicks, and the mixture were 6.2, 5.5, 1.4, and 4.0%, respectively.

An experiment was conducted to 1) test the hypothesis that a minimum adaptation period to diets used in ileal amino acid (AA) digestibility experiments with pigs is needed and 2) to test the null-hypothesis that ileal digestibility and basal endogenous losses of AA are not affected by the indigestible marker used. Eight ileal-cannulated barrows with an initial BW of 58.1 ± 4.3 kg were randomly allotted to a 2-period crossover design with 2 diets and 4 pigs per diet in each period. A soybean meal-based diet and an N-free diet were prepared. Both diets contained 0.4% chromium oxide, 0.4% titanium dioxide, and 0.4% Celite (a source of acid insoluble ash; AIA). Pigs were provided feed in a daily amount of 3 times the maintenance requirement for metabolizable energy, and 2 equal meals were provided each day. Ileal digesta samples were collected from 0800 to 2000 h on each day during the two 9-d collection periods. There was no period by diet interactions observed. Marker concentrations in ileal digesta were analyzed separately for each day, and the point where the concentration of each marker was stabilized in the digesta was determined using a linear broken-line analysis. For pigs fed the soybean meal diet, the breakpoints for Cr, Ti, and AIA in ileal digesta were 2.70, 2.45, and 3.77 d, respectively. In pigs fed the N-free diet, the breakpoints for Cr, Ti, and AIA in ileal digesta were 2.52, 2.39, and 2.29 d, respectively. Based on the pooled data, the basal endogenous losses of most AA calculated using Cr as an indigestible marker were less (P < 0.05) than the values calculated using Ti, but greater (P < 0.05) compared with values calculated based on AIA. The standardized ileal digestibility of most AA in soybean meal calculated using Cr or Ti as a digestibility marker were greater (P < 0.05) than the digestibility values calculated using AIA. In conclusion, 3 d of adaptation is required before markers are stabilized in the ileal outflow if Cr or Ti is used as an indigestible marker and 4 d of adaptation is required if AIA is the marker. Values for AA digestibility calculated using Cr or Ti as the marker are not different, but greater compared with values calculated using AIA as the marker.

The objectives were to determine the apparent ileal digestibility and standardized ileal digestibility (SID) of CP and AA in feed ingredients and to develop prediction equations for estimating SID of feed ingredient for commercial pigs using data from mini pigs. Six 11-week-old crossbred commercial pigs (31.5 ± 1.6 kg of BW; Landrace × Yorkshire) and six 20-week-old mini pigs (31.0 ± 3.2 kg BW; Jeju Island’s native pig) were individually housed in pens and all pigs were surgically fitted with a T-cannula at the distal ileum. Each pig breed was assigned to 5 dietary treatments in a 5 × 10 incomplete Latin square design involving 10 periods, respectively. Four diets were prepared to contain soybean meal, corn gluten feed, copra meal, and sesame expellers as the sole source of N. An N-free diet was formulated to estimate basal ileal endogenous losses of CP and AA. There were no interactions between breed and feed ingredient for the digestibility of CP and AA. The digestibility of CP and AA, except Arg, His, Val, and Pro, were not different between mini pigs and commercial pigs. Prediction equations for SID CP and AA of commercial pigs using SID value of mini pigs were: SID of CP (%) = (1.02 × SID of CP in mini pigs) – 5.20 with r2 = 0.97 and P < 0.05; SID of Lys (%) = (1.12 × SID of Lys in mini pigs) – 9.10 with r2 = 0.98 and P < 0.05; and SID of Met (%) = (1.08 × SID of Met in mini pigs) – 4.27 with r2 = 0.96 and P < 0.05. In conclusion, Jeju Island’s native mini pigs can be used for estimating AA digestibility in feed ingredients for commercial pigs.

The objectives were to determine the apparent total tract digestibility (ATTD) and standardized total tract digestibility (STTD) of phosphorus (P) in feed ingredients and to develop prediction equations for estimating ATTD and STTD of P in feed ingredients for commercial pigs using data from mini pigs. Twelve 13-week-old commercial pigs (42.8 ± 4.4 kg BW; Landrace × Yorkshire) and twelve 33-week-old mini pigs (66.8 ± 9.3 kg BW; Jeju Island’s native pig) were allotted to a replicated 6 × 5 incomplete Latin square design, respectively, and the pigs were individually housed in metabolic crates. Five diets were prepared to contain soybean meal (SBM), corn gluten feed (CGF), copra meal (CM), palm kernel meal (PKM), and sesame expellers (SEP) as the sole source of P. A P-free diet was formulated to estimate basal endogenous losses of P. The ATTD of P in SBM, CGF, CM, PKE, and SEP were 22.7, 23.5, 34.9, 37.6, and 8.3% in mini pigs and 30.6, 29.5, 57.3, 45.9, and 19.7% in commercial pigs. The STTD P of SBM, CGF, CM, PKE, and SEP were 41.5, 39.8, 60.0, 64.4, and 22.3% in mini pigs, and 40.9, 35.3, 71.4, 60.5, and 27.4% in commercial pigs. The mini pigs and commercial pigs showed a strong positive correlation for both ATTD (r = 0.91) and STTD P (r = 0.94). Prediction equations for ATTD and STTD of P of commercial pigs were developed: ATTD of P (%) = (1.159 × ATTD of P in mini pigs) + 7.17 with r2 = 0.82 and P < 0.05; STTD of P (%) = (0.9882 × STTD of P in mini pigs) + 2.64 with r2 = 0.88 and P < 0.05. In conclusion, Jeju Island’s native mini pigs can be used for estimating P digestibility in feed ingredients for commercial pigs.

The objective was to measure energy concentrations and standardized total tract digestibility (STTD) of phosphorus (P) in hatchery byproducts. In Experiment 1, 20 nursery barrows were used to measure energy concentrations in hatchery byproducts. A basal diet based on corn and dried whey and four additional diets containing 25% of infertile eggs, unhatched eggs, culled chicks, or a mixture of the three hatchery byproducts were prepared. In Experiment 2, the STTD of P was measured using 20 nursery barrows. Four diets containing 25% of the same hatchery byproducts used in Experiment 1 as the sole source of P were prepared, and a P-free diet was prepared to measure basal endogenous losses of P. The marker-to-marker method was employed for total collection. Metabolizable energy in culled chicks was the greatest (4560 kcal/kg as-is basis, p &lt, 0.05), whereas infertile eggs had the lowest value (2645 kcal/kg as-is basis, 0.05). The STTD of P in infertile eggs (81.7%) was greater than that in unhatched eggs, culled chicks, and the mixture (61.6, 53.9, and 47.4%, respectively, 0.05). In conclusion, culled chicks had the greatest metabolizable energy and infertile eggs had the greatest phosphorus digestibility among the test ingredients.