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La maîtrise des marqueurs de modalité est nécessaire pour les apprenants de langues étrangères. En contexte universitaire, la modalité est particulièrement importante dans la production écrite, dans la mesure où elle permet au scripteur de se positionner par rapport à ses propos. Or, l’enseignement/apprentissage des marqueurs de modalité est un défi considérable : la notion est souvent absente du matériel pédagogique, et les enseignants sont mal préparés à se confronter à l’exercice. L’analyse des interactions recueillies dans le cadre d’une formation continue en ligne pour des enseignants de FLE en Turquie permet de dégager des traits récurrents dans les discours des enseignants. Ces discours constituent une fenêtre vers l’agir professoral, et permettent d’entrevoir les attitudes et pratiques des enseignants sur les obstacles relatifs à l’enseignement de la modalité. The appropriate use of modality markers is crucial for foreign language learners. In an academic context, modality is particularly important in writing, insofar as it allows writers to express their stance in relation to their propositions. However, teaching modality markers constitutes a considerable challenge, which language teachers are ill-prepared to undertake. By analyzing L2 French teachers’ interactions in the context of an online in-service training course in Turkey, this study aims to identify recurring features in their discourse. Teachers’ discourse in training contexts constitute a window towards teacher cognition and help to better understand their views on the obstacles related to the teaching of modality.

Purpose: We undertook this study to determine the prevalence of estrogen receptor (ER) α (ESR1) mutations throughout the natural history of hormone-dependent breast cancer and to delineate the functional roles of the most commonly detected alterations.Experimental Design: We studied a total of 249 tumor specimens from 208 patients. The specimens include 134 ER-positive (ER+/HER2−) and, as controls, 115 ER-negative (ER−) tumors. The ER+ samples consist of 58 primary breast cancers and 76 metastatic samples. All tumors were sequenced to high unique coverage using next-generation sequencing targeting the coding sequence of the estrogen receptor and an additional 182 cancer-related genes.Results: Recurring somatic mutations in codons 537 and 538 within the ligand-binding domain of ER were detected in ER+ metastatic disease. Overall, the frequency of these mutations was 12% [9/76; 95% confidence interval (CI), 6%–21%] in metastatic tumors and in a subgroup of patients who received an average of 7 lines of treatment the frequency was 20% (5/25; 95% CI, 7%–41%). These mutations were not detected in primary or treatment-naïve ER+ cancer or in any stage of ER− disease. Functional studies in cell line models demonstrate that these mutations render estrogen receptor constitutive activity and confer partial resistance to currently available endocrine treatments.Conclusions: In this study, we show evidence for the temporal selection of functional ESR1 mutations as potential drivers of endocrine resistance during the progression of ER+ breast cancer. Clin Cancer Res; 20(7); 1757–67. ©2014 AACR.

PDF file - 187K, Supplementary Table 1: Detailed demographic information of all patients with metastatic breast cancer, including cohorts LM+ and EM+. Supplementary Table 2: ESR1 variant sequence data details. Abbreviations: cvg, coverage, N/A, non-applicable. Supplementary Table 3: List of genes sequenced by captured next generation sequencing. Supplementary figure 1: Genomic profiles of primary and metastatic tumors. Genomic alterations were found in 32 genes in primary and metastatic ER+ breast cancers. Genes are listed from the most frequently altered to least altered gene. Supplementary figure 2: No change in WT and mutant ER activity across a wide range of E2 doses. Luciferase activity in 293T cells after co-transfection of the ERE-TK-Luc reporter vector along with WT-ER, Y537N or D538G and E2 stimulation using doses of E2 ranging from 0.01nM to 100nM or vehicle treatment.

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The lifespans of proteins can range from minutes to years within mammalian tissues. Protein lifespan is relevant to organismal aging, as long-lived proteins can accrue damage over time. It is unclear how protein lifetime is shaped by tissue context, where both cell division and proteolytic degradation contribute to protein turnover. Here, we develop turnover and replicationanalysis by15N isotope labeling (TRAIL) to quantify both protein and cell lifetimes with high precision and no toxicity over a 32-day labeling period across 4 mammalian tissues. We report that cell division promotes non-selective protein turnover in proliferative tissues, while physicochemical features such as hydrophobicity, charge, and intrinsic disorder exert a significant influence on protein turnover only in non-proliferative tissues. Protein lifetimes vary non-randomly across tissues after correcting for differences in cell division rate. Multiprotein complexes such as the ribosome have highly consistent lifetimes across tissues, while mitochondria, peroxisomes, and lipid droplets have variable lifetimes. These data indicate that cell turnover, sequence-encoded features, and other environmental factors modulate protein lifespanin vivo. In the future, TRAIL can be used to explore how environment, aging, and disease affect tissue homeostasis.

Mutations to theLMNAgene cause laminopathies including Hutchinson-Gilford progeria syndrome (HGPS) that severely affect the cardiovascular system. The origins of tissue specificity in these diseases are unclear, as the A-type Lamins are abundant and broadly expressed proteins. We show that A-type Lamin protein and transcript levels are uncorrelated across tissues. As protein-transcript discordance can be caused by variations in protein lifetime, we applied quantitative proteomics to profile protein turnover rates in healthy and progeroid tissues. We discover that tissue context and disease mutation each influence A-type Lamin protein lifetime. Lamin A/C has a weeks-long lifetime in the aorta, heart, and fat, where progeroid pathology is apparent, but a days-long lifetime in the liver and gastrointestinal tract, which are spared from disease. The A-type Lamins are insoluble and densely bundled in cardiovascular tissues, which may present an energetic barrier to degradation and promote long protein lifetime. Progerin is even more long-lived than Lamin A/C in the cardiovascular system and accumulates there over time. Progerin accumulation interferes broadly with protein homeostasis, as hundreds of abundant proteins turn over more slowly in progeroid tissues. These findings indicate that potential gene therapy interventions for HGPS will have significant latency and limited potency in disrupting the long-lived Progerin protein. Finally, we reveal that human disease alleles are significantly over-represented in the long-lived proteome, indicating that long protein lifetime may influence disease pathology and present a significant barrier to gene therapies for numerous human diseases.Significance statementMany human diseases are caused by mutations to broadly expressed proteins, yet disease mysteriously manifests only in specific tissues. An example of this is Hutchinson-Gilford progeria syndrome (HGPS), which is caused by a mutation to the Lamin A/C protein. We show that this mutation slows the turnover of Lamin A/C proteins in disease-afflicted tissues, causing the mutant “Progerin” protein to accumulate over time and interfere with the normal turnover of hundreds of other proteins. Because Progerin is a long-lived protein, effective therapies for this disease will need to attack the protein and not just the gene that encodes it.

Cet ouvrage dirigé par Caroline Scheepers, spécialiste des littératies scolaire et universitaire à l’Université libre de Bruxelles, est une compilation riche et diverse de perspectives complémentaires sur la place de l’écrit dans l’enseignement supérieur dans des contextes francophones. L’ouvrage est ambitieux par son envergure thématique et géographique. Il repousse les limites du champ de recherche sur l’écrit universitaire, et s’enrichit des apports des recherches sur des terrains francoph...

Turkish-speaking L2 French learners face specific difficulties in writing. Some of these difficulties may be related to the influence of L1. The aim of our research is to elucidate the differences and similarities between certain writing patterns in French and Turkish, in order to try to understand the extent to which L1 writing patterns and grammar are likely to have an impact on written production in EFL.For this purpose, we have constituted a corpus of native and learner writers. The corpus is composed of four groups of writers: native French speakers, native Turkish speakers, Turkish speakers learning FLE at B1 level, and learners at B2 level. For each group of writers, the corpus consists of texts belonging to two different genres: formal letters and e-mails.We propose, in this presentation, to describe the methodological challenges related to the constitution of this corpus under three axes: LongitudinalityRepresentativenessDesigning the writing tasksWe will discuss the solutions to these issues. While it is not possible to find a solution to all of these challenges and biases, we believe that an awareness of the issues helps to mitigate their impact on the research results. This type of reflection can contribute to reinforcing good practices for the constitution of a written corpus in line with the work of the CORLI consortium, which proposes concrete recommendations for spoken corpora.

This paper examines how the intercultural conception of human rights, fueled by the modes of reciprocal recognition associated with Hegel’s social philosophy, draws on traditional understandings of human dignity while avoiding the essentialism associated with those understandings. Part 1 summarizes core elements of an intercultural theory of human rights while addressing the general question of how that theory accommodates an understanding of the relationship of human dignity and human rights. Part 2 presents the intercultural approach as committed to a view of human dignity focused on the intrinsic worth of individuals, but understood, not—with Kant—as an anthropologically inherent property, but as one forged in relations of intersubjective recognition. Part 3, critically engaging arguments of C Beitz and J Habermas, claims that an intercultural approach entails a status-based conception of human dignity, one that construes the latter, not as a metaphysically conceived inherent quality, but as a function of membership in a social order supportive of both liberal right claims and a republican commitment to rights systems generally. Drawing on the work of Hegel and critically engaging the position of A Sangiovanni, Part 4 construes human dignity in terms of a doctrine of human rights expressive of a common humanity, one understood, however, not as an essential attribute of human beings, but in terms of a globally realized legal-political order whose members are dispositionally committed to the worth of all members of the human community and the value of human community itself. Critically appropriating the work of H Arendt for the intercultural theory, Part 5 considers the centrality of actual politics to a dignitarian account of human rights. Part 6 construes human dignity, again with Kant, in terms of the principle of autonomy, but one understood, not abstractly, but—engaging Pico della Mirandola—as the fallibilistically conceived process of social learning on the part the members of the human community. Part 7 considers how the intercultural view, with the idea of social practice expressive of collective human agency, accommodates a concept of human dignity understood as the “foundation”—nuanced and variegated—of human rights. Part 8 details the specific normativity of an intercultural conception, asserting that despite and even because of its attention to the empirical realities of social, historical, and political life, that conception remains committed, as with essentialist views, to context-transcending norms capable of calling into question actual practices and policies. Here, recourse is had to a concept of recognition understood both as a principle of evaluation and as a social practice encompassing the activity of individuals and groups engaged in the discourse on human rights.

Relative to a growing body of knowledge about the negative consequences of freshwater salinization, little is known about how aquatic insects respond to progressively ion-poor conditions. Here, we examined life-history and physiological acclimation in

Relative to a growing body of knowledge about the negative consequences of freshwater salinization, little is known about how aquatic insects respond to progressively ion-poor conditions. Here, we examined life-history and physiological acclimation in Neocloeon triangulifer by rearing nymphs from 1-day post-egg hatch to adulthood across a gradient of decreasing Na concentrations (15, 8, 4, 2 and 1 mg l −1 Na). We found no significant changes in survival, growth, development time and whole-body Na content across these treatments. Radiotracer data revealed that nymphs acclimated to their dilute exposures by increasing their rates of Na uptake and were able to maintain a relatively narrow range of uptake rates (±s.e.m.) of 38.5 ± 4.2 µg Na g −1 h −1 across all treatments. By contrast, the Na uptake rates observed in naive nymphs were much more concentration dependent. This acclimatory response is partially explained by differences in ionocyte counts on the gills of nymphs reared under different salinities. Acclimated nymphs were surprisingly less retentive of their sodium composition when subjected to deionized water challenge. By contrasting our findings with a previous N. triangulifer salinity acclimation study, we show a physiological affinity for dilute conditions in this emerging mayfly model.

Aging is the single largest risk factor for most chronic diseases, and thus possesses large socioeconomic interest to continuously aging societies. Consequently, the field of aging research is expanding alongside a growing focus from the industry and investors in aging research. This year's 8th Annual Aging Research and Drug Discovery (ARDD) meeting was organized as a hybrid meeting from August 30th to September 3rd 2021 with more than 130 attendees participating on-site at the Ceremonial Hall at University of Copenhagen, Denmark, and 1800 engaging online. The conference comprised of presentations from 75 speakers focusing on new research in topics including mechanisms of aging and how these can be modulated as well as the use of AI and new standards of practices within aging research. This year, a longevity workshop was included to build stronger connections with the clinical community., Aging, 14 (2), ISSN:1945-4589

Raw counts of ionocytes

This essay evaluates Corradetti’s compelling assertion that the “primary aim” of his Relativism and Human Rights “is that of reconstructing some central concepts of the Hegelian understanding of the right and of providing a post-metaphysical reinterpretation of the notion of recognition in terms of a dialectical process, granting fundamental rights within the context of an institutional discursive model of rationality.” The discussion is divided into four parts. Part 1 reviews Hegel’s reception of the tradition of natural right, asserting that the latter plays a role in Hegel’s recognitive account of ethical life greater than Corradetti allows. Part 2 considers the use Corradetti makes of Hegel’s concept of dialectical negativity, arguing that, while it can and should be understood in terms of relations of recognition and, especially, misrecognition, it also articulates features of Hegel’s logic and metaphysics that – pace Corradetti – can play a role in a postmetaphysical theory of social life. Part 3 seconds Corradetti’s claims about the value of recognition theory for a Hegelian account of universal human rights, but asserts that that value is best expressed via an intercultural understanding of that account. Part 4 details the normative dimension of such an account, arguing that here, too, Hegel has more to offer than Corradetti allows.

Ecologists have observed declines in the biodiversity of sensitive freshwater organisms in response to increasing concentrations of major ions (salinization). Yet, how changing salinities physiologically challenge aquatic organisms, such as mayflies, remains remarkably understudied. Moreover, it is not well understood the degree to which species respond and acclimate to salinity changes. Our lab is developing the Baetid mayfly, N. triangulifer, as a model organism for physiological research. We have previously described acclimatory changes in both ion flux rates and altered mRNA transcript levels in response to chronic exposures to elevated major ion concentrations at the whole animal level. In the present study, we use shotgun proteomics to identify the specific proteins associated with apical ion transport and how their abundance changes in response to chronic salinity exposures in gills. Gills were isolated from the penultimate nymphal stage of N. triangulifer reared under control culture conditions, elevated NaCl (157 mg L

The lifespans of proteins can range from moments to years within mammalian tissues. Protein lifespan is relevant to organismal aging, as long-lived proteins can accrue damage over time. It is unclear how protein lifetime is shaped by tissue context, where both cell division and proteolytic degradation contribute to protein turnover. We have developed turnover and replication analysis by 15N isotope labeling (TRAIL) for parallel quantification of protein and cell lifetimes. We have deployed TRAIL over 32 days in 4 mouse tissues to date to quantify cell proliferation with high precision and no toxicity and determine that protein lifespan varies independently of cell lifespan. Variation in protein lifetime is non-random: multiprotein complexes such as the ribosome have consistent lifetimes across tissues, while mitochondria, peroxisomes, and lipid droplets have variable lifetimes across tissues. To model the effects of aging on tissue homeostasis, we apply TRAIL to a mouse model of Hutchinson-Gilford progeria syndrome and uncover fat-specific alterations in cell lifetime and proteome composition, as well as a broad decrease in protein turnover flux. These data indicate that environmental factors influence protein turnover in vivo and provide a framework to understand proteome aging in tissue context.

Hypoxia is a growing concern in aquatic ecosystems. Historically, scientists have used the P

Increased temperatures and exposure to agricultural insecticides are increasingly threatening freshwater ecosystems worldwide. However, their combined effects are still poorly understood. We invest...

Freshwater salinization is a rapidly emerging ecological issue and is correlated with significant declines in aquatic biodiversity. It remains unclear how changing salinity regimes affect the physiology of sensitive aquatic insects. We used the parthenogenetic mayfly, Neocloeon triangulifer, to ask how ionic exposure history alters physiological processes and responses to subsequent major ion exposures. Using radiotracers (22Na, 35SO4, and 45Ca), we observed that mayflies chronically reared in elevated sodium or sulfate (157 mg L−1 Na or 667 mg L−1 SO4) had 2-fold (p

To better understand the effects of transient thermal stress in an aquatic insect, we first identified static temperatures associated with fitness deficits, and then reared larvae from egg hatch to adulthood under diurnally variable regimens including daily forays into deleterious temperatures. We sampled mature larvae at the coolest and warmest portions of their respective regimens for RNA-seq analysis. Few transcripts (28) were differentially expressed when larvae oscillated between favorable temperatures, while 614 transcripts were differentially expressed when experiencing daily transient thermal stress. Transcripts associated with N-glycan processing were downregulated while those associated with lipid catabolism and chitin turnover were significantly upregulated in heat stressed larvae. An across-regimen comparison of differentially expressed transcripts among organisms sampled at comparable temperatures demonstrated that the effects of daily thermal stress persisted even when larvae were sampled at a more optimal temperature (806 differentially expressed transcripts). The chronically stressed population had reduced expression of transcripts related to ATP synthesis, mitochondrial electron chain functions, gluconeogenesis and glycolytic processes while transcripts associated with cell adhesion, synaptic vesicle transport, regulation of membrane potential and lipid biosynthesis increased. Comparisons of constant vs. variable temperatures revealed that the negative consequences of time spent at stressful temperatures were not offset by more time spent at optimal temperatures.

Periphyton is ubiquitous in rice paddy fields, however its role in paddy soil arsenic (As) biogeochemistry remains unexplored. In this study, microcosm incubations and extensive field sampling were used to better understand the roles of periphyton on As mobility and transformation at the soil-water interface. Microcosm incubations revealed that periphyton on the paddy soil surface enhanced As release to water and increased methylated As contents at the soil-water interface. Experimental additions of dissolved phosphate did not significantly affect these processes. The presence of periphyton increased the dissolved organic carbon (DOC) content of the surface soil which may have played a role in the increased As mobility. However, the increase in methylated As species at the soil-water interface is indicative of detoxification processes of As by periphyton. The results from the field study revealed a high abundance and diversity of As biotransformation and detoxification genes in periphyton. Genera of Kineosporia, Limisphaera, Ornatilinea, Ktedonosporobacter and Anaerolinea played key roles in shaping arsM harboring microbe communities in field periphyton. These results highlight the importance of periphyton in the behavior of As in paddy soils and can potentially facilitate improved management of As contamination in paddy soils.

The genes that code for ribosomal RNA are present in hundreds of tandemly arrayed copies in the human genome. Ribosomal DNA repeats transcribe vast amounts of ribosomal RNA in order to meet the cell's relentless demand for ribosome production. Intrinsic features of ribosomal DNA repeats render them uniquely vulnerable to DNA damage. Sensing and repairing damage to ribosomal DNA involves dramatic spatial reorganization of the nucleolus, the phase-separated nuclear subdomain where ribosomes are made. We highlight recent advances in detecting the incidence of DNA damage and defining the mechanisms of DNA repair on these essential genes.

Hypoxia is a growing concern in aquatic ecosystems. Historically, scientists have used the Pcrit (the dissolved oxygen level below which an animal can no longer oxyregulate) to infer hypoxia tolerance across species. Here, we tested the hypothesis that the Pcrit is positively correlated with temperature in the mayfly, Neocloeon triangulifer. Cross-temperature comparisons showed a modest (r = 0.47), but significant (p

We reared Cloeon dipterum from egg hatch to adult at 10 constant temperatures (12.1–33.5°C) to test 3 hypotheses (thermal equilibrium hypothesis, temperature size rule [TSR], and O2- and ca...

OBJECTIVE Human obesity is a complex metabolic disorder disproportionately affecting people of lower socioeconomic strata, and ethnic minorities, especially African Americans and Hispanics. Although genetic predisposition and a positive energy balance are implicated in obesity, these factors alone do not account for the excess prevalence of obesity in lower socioeconomic populations. Therefore, environmental factors, including exposure to pesticides, heavy metals, and other contaminants, are agents widely suspected to have obesogenic activity, and they also are spatially correlated with lower socioeconomic status. Our study investigates the causal relationship between exposure to the heavy metal, cadmium (Cd), and obesity in a cohort of children and in a zebrafish model of adipogenesis. DESIGN An extensive collection of first trimester maternal blood samples obtained as part of the Newborn Epigenetics Study (NEST) was analyzed for the presence of Cd, and these results were cross analyzed with the weight-gain trajectory of the children through age five years. Next, the role of Cd as a potential obesogen was analyzed in an in vivo zebrafish model. RESULTS Our analysis indicates that the presence of Cd in maternal blood during pregnancy is associated with increased risk of juvenile obesity in the offspring, independent of other variables, including lead (Pb) and smoking status. Our results are recapitulated in a zebrafish model, in which exposure to Cd at levels approximating those observed in the NEST study is associated with increased adiposity. CONCLUSION Our findings identify Cd as a potential human obesogen. Moreover, these observations are recapitulated in a zebrafish model, suggesting that the underlying mechanisms may be evolutionarily conserved, and that zebrafish may be a valuable model for uncovering pathways leading to Cd-mediated obesity in human populations.

Crystal structures of two benzo­thia­zinones belonging to a class of compounds implicated in anti­microbial, anti­tumour, and HIV-RT inhibitory activity are reported., The syntheses and crystal structures of 2,3-bis­[3-(tri­fluoro­meth­yl)phen­yl]-2,3-di­hydro-4H-1,3-benzo­thia­zin-4-one (di-m-CF3; C22H13F6NOS) (1) and 2,3-bis­(4-methyl­phen­yl)-2,3-di­hydro-4H-1,3-benzo­thia­zin-4-one (di-p-CH3; C22H19NOS) (2) are reported. Each structure is racemic: the asymmetric unit of 2 consists of two mol­ecules. In both 1 and 2, the six-membered 1,3-thia­zine ring is close to an envelope conformation with the chiral C atom forming the flap. In 1, the 2-aryl group is pseudo-equatorial, while in 2 it is pseudo-axial. In 1, the pendant aryl rings form a V shape with an inter-centroid distance of 3.938 (3) Å and an acute dihedral angle of 48.3 (2)° between them. Both CF3 groups are disordered over two orientations in 0.687 (19):0.313 (19) and 0.667 (16):0.33 (16) ratios. In each of the independent mol­ecules of 2, the aryl rings are almost orthogonal to each other [dihedral angles = 85.50 (12) and 86.07 (11)°]. In both structures, the chiral C atom and the O atoms participate in C—H⋯O-type hydrogen bonding between symmetry-related mol­ecules of 1 or the independent enanti­omers in 2, forming chains along the c-axis direction in 1 and the b-axis direction in 2. Additionally, in 1, π–π contacts of both face-to-face and edge-to-face type, as well as π-H⋯O and π-H⋯F inter­actions are observed. In 2, a racemic mixture of mol­ecules forms layers in the ac plane linked by weak π–π and C—H⋯π inter­actions.

The Utica/Point Pleasant Formations comprise a major unconventional gas resource in the Appalachian Basin. Core samples from several boreholes in Ohio, West Virginia and Pennsylvania were studied to determine mineralogy, microtexture/microfabric, accessible pore surface area, porosity and pore size distribution as a function of maturity in the Utica/Point Pleasant sub-basin. This effort compares shallower, generally more phyllosilicate-rich Utica intervals with deeper, more carbonate-rich Point Pleasant intervals, the latter representative of horizons currently targeted for gas recovery. The Point Pleasant Formation contains mostly calcite (in the form of fossil tests, grains, and cements), distributed within alternating carbonate- and phyllosilicate-rich matrix laminae, as well as coarse-grained fossil-rich laminae. In both Utica and Point Pleasant core samples, the greatest mercury-accessible connected porosity measurements are associated with the highest maturity samples, an observation that is particularly pronounced for the Point Pleasant. More detailed vertical sampling and analysis of core from a well in Harrison County, Ohio (a region that has experienced copious shale gas production) shows that surface area, pore volume and pore connectivity increase locally in target horizons identified for gas recovery. Observations suggest that optimal mixtures and arrangements of minerals and organic matter, as well as increasing maturity, contribute to forming this productive carbonate-rich unconventional resource. In particular, early diagenetic calcite and quartz cement and increasing quantities of silt-sized particles are associated with higher measured connected porosities, while later-stage matrix dolomitization and recrystallization of fossil laminae tend to occlude the pore network.

Zika virus (ZIKV) became a global health concern in 2016 due to its links to congenital microcephaly and other birth defects. Flaviviruses, including ZIKV, reorganize the endoplasmic reticulum (ER) to form a viroplasm, a compartment where virus particles are assembled. Microtubules (MTs) and microtubule-organizing centers (MTOCs) coordinate structural and trafficking functions in the cell, and MTs also support replication of flaviviruses. Here we investigated the roles of MTs and the cell’s MTOCs on ZIKV viroplasm organization and virus production. We show that a toroidal-shaped viroplasm forms upon ZIKV infection, and MTs are organized at the viroplasm core and surrounding the viroplasm. We show that MTs are necessary for viroplasm organization and impact infectious virus production. In addition, the centrosome and the Golgi MTOC are closely associated with the viroplasm, and the centrosome coordinates the organization of the ZIKV viroplasm toroidal structure. Surprisingly, viroplasm formation and virus production are not significantly impaired when infected cells have no centrosomes and impaired Golgi MTOC, and we show that MTs are anchored to the viroplasm surface in these cells. We propose that the viroplasm is a site of MT organization, and the MTs organized at the viroplasm are sufficient for efficient virus production.

Benthic periphytic biofilms are important food sources at the base of aquatic ecosystems. These biofilms also sit at the interface of oxic waters and hypoxic sediments, and can be influenced by or influence trace element speciation. In the present study, we compared arsenic (As) enrichment in periphyton exposed to arsenate (As[V]) or arsenite (As[III]) (20 μg/L, static renewal, 7 d), and we found similar accumulation patterns of total As (101 ± 27 and 88 ± 22 mg kg−1 dry wt, respectively). Periphyton As was 6281- and 6684-fold higher than their aqueous exposures and occurred primarily as As(V). When these biofilms were fed to larval mayflies, similar total As tissue concentrations (13.9 and 14.6 mg kg−1 dry wt, respectively) were observed, revealing significant biodilution (∼ 10% of their dietary concentrations). Finally, we investigated the influence of aeration and periphyton presence on As speciation in solutions and solid phases treated with As(III). Predominantly As(III) solutions were slowly oxidized over a 7-d time period, in the absence of periphyton, and aeration did not strongly affect oxidation rates. However, in the presence of periphyton, solution and solid-phase analyses (by microscale x-ray absorption spectroscopy) showed rapid As(III) oxidation to As(V) and an increasing proportion of organo-As forming over time. Thus periphyton plays several roles in As environmental behavior: 1) decreasing total dissolved As concentrations via abiotic and biotic accumulation, 2) rapidly oxidizing As(III) to As(V), 3) effluxing organo-As forms into solution, and 4) limiting trophic transfer to aquatic grazers. Environ Toxicol Chem 2017;9999:1–11. © 2017 SETAC

Despite baccalaureates in criminal justice (BCJ) being among the most awarded degrees in this country, little published research has appeared in recent years on these programs or their curricula. Using 2015–2016 data collected from the population of BCJ-granting institutions (N = 670) in the U.S., we partially replicated Southerland’s 2002 Criminal justice curricula in the United States: A decade of Change and extended Sloan and Buchwalter’s 2016 The state of bachelor’s degree programs in the United States: Institutional, department, and curricula features analyses of BCJ curricula by comparing selected features of them across three groups of colleges/universities: public, private, not-for-profit, and private, for-profit. Results revealed significant differences existed in curricula by institutional locus of control, indicating a general lack of consensus on undergraduate curricula, including in such fundamental areas as total hours needed for the major. We discuss the implications of these results for th...

While for-profit colleges and universities (FPCUs) have long existed in the U.S., most were small, closely held companies (CHCs). Beginning in the 1990s, a new FPCU emerged that was owned by publicly traded companies (PTCs). While FPCUs owned by CHCs and PTCs offer baccalaureates in criminal justice (BCJ), little is known about the characteristics of these programs. To address this gap, the current study analyzed the population (N = 79) of FPCUs offering BCJ programs during academic year 2015–2016 by type of corporate owner. Analyses included comparisons of the two types of FPCUs on three categories of variables: institutional, departmental, and curricular characteristics. Implications of the results for baccalaureate education in criminal justice and future research on the role of FPCUs in criminal justice are presented.

The salinization of freshwater ecosystems is emerging as a major ecological issue. Several anthropogenic causes of salinization (e.g. surface coal mining, hydro-fracking, road de-icing, irrigation of arid lands, etc.) are associated with biodiversity losses in freshwater ecosystems. Because insects tend to dominate freshwater ecology, it is important that we develop a better understanding of how and why different species respond to salinity matrices dominated by different major ions. This study builds upon previous work demonstrating that major ion toxicity to the mayfly Neocloeon triangulifer was apparently due to the ionic composition of water rather than specific conductance. Synthetic waters with low Ca:Mg ratios and high SO4:Na ratios produced toxicity, whereas waters with higher Ca:Mg ratios and lower SO4:Na ratios were not toxic to mayflies at comparable conductivities. Here we used a radiotracer approach to show that Mg did not competitively exclude Ca uptake at environmentally realistic ratios in 4 aquatic insect species. We characterized SO4 uptake kinetics in 5 mayflies and assessed the influence of different ions on SO4 uptake. Dual label experiments show an inverse relationship between SO4 and Na transport rates as SO4 was held constant and Na was increased, suggesting that Na (and not Cl or HCO3) is antagonistic to SO4 transport. Based on this observation, we tested the hypothesis that increasing Na would protect against SO4 induced toxicity in a Na-dependent manner. Increasing Na from 0.7 to 10.9mM improved 96-h survivorship associated with 20.8mM SO4 from 44% to 73% in a concentration dependent manner. However, when Na reached 21.8mM, survivorship decreased to 16%, suggesting that other interactive effects of major ions caused toxicity under those conditions. Thus, the combination of elevated sulfate and low sodium commonly observed in streams affected by mountaintop coal mining has the potential to cause toxicity in sensitive aquatic insects. Overall, it is important that we develop a better understanding of major ion toxicity to effectively mitigate and protect freshwater biodiversity from salinization.

Premature aging disorders provide an opportunity to study the mechanisms that drive aging. In Hutchinson-Gilford progeria syndrome (HGPS), a mutant form of the nuclear scaffold protein lamin A distorts nuclei and sequesters nuclear proteins. We sought to investigate protein homeostasis in this disease. Here, we report a widespread increase in protein turnover in HGPS-derived cells compared to normal cells. We determine that global protein synthesis is elevated as a consequence of activated nucleoli and enhanced ribosome biogenesis in HGPS-derived fibroblasts. Depleting normal lamin A or inducing mutant lamin A expression are each sufficient to drive nucleolar expansion. We further show that nucleolar size correlates with donor age in primary fibroblasts derived from healthy individuals and that ribosomal RNA production increases with age, indicating that nucleolar size and activity can serve as aging biomarkers. While limiting ribosome biogenesis extends lifespan in several systems, we show that increased ribosome biogenesis and activity are a hallmark of premature aging., HGPS is a premature aging disease caused by mutations in the nuclear protein lamin A. Here, the authors show that cells from patients with HGPS have expanded nucleoli and increased protein synthesis, and report that nucleoli also expand as aging progresses in cells derived from healthy individuals.