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Let L be a commutative unital quantale. For every L-fuzzy relation E on a nonempty set X, we define an upper rough approximation operator on LX, which is a fuzzy extension of the classical Pawlak upper rough approximation operator. We show that this operator has close relation with the subsethood operator on X. Conversely, by an L-fuzzy closure operator on X, we can easily get an L-fuzzy relation. We show that this relation can be characterized by more smooth ways. Without the help of the lower approximation operator, L-fuzzy rough sets can still be studied by means of constructive and axiomatic approaches, and L-fuzzy similarities and L-fuzzy closure operators are one-to-one corresponding. We also show that, the L-topology induced by the upper rough approximation operator is stratified and Alexandrov.

Various applications of oil palm empty fruit bunches (OPEFB) would be hindered by the presence of residual oil. This study aimed to remove and recover the residual oil from OPEFB using an integrated system, high pressure water spray system (HPWSS). The performance of the HPWSS was evaluated at different temperatures and water pressures, and the residual oil collected was recovered through water shaking method and tested for biodiesel application. A maximum of 84.9% of residual oil was removed by HPWSS at 60 °C and 8960 kPa and the highest residual oil recovery of 58.8% was observed at 95 °C, using power shaking 5 and 90% of dilution. The following ranges of deterioration of bleachability index (DOBI), free fatty acid (FFA), and peroxide value (PV) for the residual oil were 1.21 to 2.67, 7.11 to 10.4%, and 4.85 to 7.56, respectively. Biodiesel with different blends of recovered residual oil (5%, 10%, and 15%) showed lower values (9.87, 9.57, and 9.56 Nm) of brake torque as compared with diesel (10.89 Nm). Overall, this study showed the potential of HPWSS to obtain an acceptable quality of residual oil from OPEFB to be used in any value-added product generation.

The structure diagram and detection mechanism of CsPbBr3/TDPA.

Silver-based organic frameworks with high ECL activity were synthesized and applied for the first time to construct an ECL sensor. The detection limit was as low as 66 fM for mercury ion concentration in water.

A novel FeNi-Mo2C@SiO2 monolith electrocatalyst was constructed to boost the OER due to the increased number of active sites and enhanced intrinsic activity.

Saline–alkaline (SA) stress is characterized by high salinity and high alkalinity (high pH), which severely inhibit plant growth and cause huge losses in crop yields worldwide. Here we show that a moderate elevation of endogenous abscisic acid (ABA) levels by RNAi-mediated suppression of OsABA8ox1 (OsABA8ox1-kd), a key ABA catabolic gene, significantly increased tolerance to SA stress in rice plants. We produced OsABA8ox1-kd lines in two different japonica cultivars, Dongdao 4 and Nipponbare. Compared with non-transgenic control plants (WT), the OsABA8ox1-kd seedlings accumulated 25.9%–55.7% higher levels of endogenous ABA and exhibited reduced plasmalemma injury, ROS accumulation and Na+/K+ ratio, and higher survival rates, under hydroponic alkaline conditions simulated by 10, 15, and 20 mmol L−1 of Na2CO3. In pot trials using SA field soils of different alkali levels (pH 7.59, 8.86, and 9.29), OsABA8ox1–kd plants showed markedly higher seedling survival rates and more vigorous plant growth, resulting in significantly higher yield components including panicle number (85.7%–128.6%), spikelets per panicle (36.9%–61.9%), branches (153.9%–236.7%), 1000–kernel weight (20.0%–28.6%), and percentage of filled spikelets (96.6%–1340.8%) at harvest time. Under severe SA soil conditions (pH = 9.29, EC = 834.4 μS cm−1), OsABA8ox1-kd lines showed an 194.5%–1090.8% increase in grain yield per plant relative to WT plants. These results suggest that suppression of OsABA8ox1 to increase endogenous ABA levels provides a new molecular approach for improving rice yield in SA paddies.

The aim of the articleis to investigate the transformation of female images in Chinese silent cinema of the first third of the 20th century in the context of the interaction of screen and stage arts. Results.An analysis of the development of cinema and theatre in the first half of the 20th century in China proves that the patriarchal heroine types of traditional theatre, who sacrifice themselves for men, are becoming obsolete due to the general liberalisation of social life. The development of spoken drama opens up opportunities for female actresses. However, the feminisation of the cast in the cinema does not happen immediately: initially, male actors played female roles, then supporting actresses appear, and only from the early 1920s, women began to play the main characters. The first generation of Chinese film actresses set an example for imitation, formed new standards of beauty and behaviour. Scientific novelty.This publication for the first time clarifies on the features of the transformation of female images in Chinese silent cinema of the first third of the 20th century in the context of the interaction of screen and stage arts. Conclusions.It is proved that the images of new women in Chinese silent cinema reproduced feminist ideals and at the same time stimulated the struggle for female emancipation. During the 1920s, the idea of moving away from the patriarchal model was consistently broadcast through screen and stage art. Even conservative filmmakers reflected changes in gender issues, as evidenced by the appearance of the penitent heroine from Neo-Confucian melodramas and the female warrior from Wuxia films. It is concluded that in the films of the silent period, different types of female images coexist: on the one hand, a wife who seeks release from heavy duties, on the other – a woman with a dubious reputation who sacrifices herself for the sake of her children. It is emphasised that the female image that adequately reflects the achievements of Chinese feminism will appear already in the era of sound cinema.

Background: The study was designed to evaluate the impact of pharmacist-led clinical interventions on the health-related quality of life among tuberculosis patients in Pakistan.Methods: A randomized, controlled prospective study was carried out in a Pakistan Institute of Medical Sciences hospital tuberculosis (TB) control center. Participants who visited the TB center between September 2020 and December 2021 were randomly assigned to two clusters, the usual care group (UC group) vs. the intervention group (pharmaceutical care group), in a 1:1 ratio by a simple envelope technique. In the intervention group, a patient received centered care that encompassed informed decision-making, which can increase the quality of care and monitoring of adverse drug events. However, the control group received routine TB treatment at the hospital. The EuroQol-5D-3L instrument was used to assess the health-related quality of life (HRQoL) at the baseline and in the third and sixth months of the treatment time period.Results: A total of 503 patients were eligible, of which only 426 patients were included in this study. At the end of the study, n = 205 of the patients in the intervention group and n = 185 of those in the control group were analyzed. In the intervention group, the EQ-5D-3L health utility score improved significantly (p < 0.001) (from the baseline mean ± SD, 0.40 ± 0.36, to 6 months of treatment, 0.89 ± 0.09, while in the control group from 0.42 ± 0.35 to 0.78 ± 0.27). In multivariate regression analysis, the variables that remained statistically associated (p < 0.001) with the HRQoL (unstandardized β [95% confidence interval]) of the control group were as follows: gender, female vs. male (-0.039 [-0.076 to -0.003]); body weight, less than 40 kg vs. more than 40 kg (-0.109 [-0.195 to -0.024]); patients with any comorbidity vs. without comorbidity (-0.136 [-0.252 to -0.020]); and smokers vs. non-smokers (-0.204 [-0.291 to -0.118]). The study did not find any statistically significant associations between the intervention group’s variables and the HRQoL.Conclusion: Patient-centered care interventions led by pharmacists as part of care coordination enhanced the HRQoL for TB patients significantly. According to this study, clinical pharmacists should be included in the interdisciplinary clinical staff for TB patient management.

Data on the distribution of voriconazole (VRC) in the human peritoneal cavity are sparse. This prospective study aimed to describe the pharmacokinetics of intravenous VRC in the peritoneal fluid of critically ill patients.

In this work, we present an automated platform for trapping and stretching individual micro- and nanoscale objects in solution using electrokinetic forces. The platform can trap objects at the stagnation point of a planar elongational electrokinetic field for long time scales, as demonstrated by the trapping of ~100 nanometer polystyrene beads and DNA molecules for minutes, with a standard deviation in displacement from the trap center < 1 micrometer. This capability enables the stretching of deformable nanoscale objects in a high-throughput fashion, as illustrated by the stretching of more than 400 DNA molecules within ~4 hours. The flexibility of the electrokinetic stretcher opens up numerous possibilities for contact-free manipulation, with size-based sorting of DNA molecules performed as an example. The platform described provides an automated, high-throughput method to track and manipulate objects for real-time studies of micro- and nanoscale systems., 9 pages, 7 figures

For a usual commutative quantale Q (does not necessarily have a unit), we propose a definition of Q-ordered sets by introducing a kind of self-adaptive self-reflexivity. We study their completeness and the related Q-modules of complete lattices. The main result is that, the complete Q-ordered sets and the Q-modules of complete lattices are categorical isomorphic.

The composite PCN-222@CdSe was used to detect PNP.

The Witten effect implies the electromagnetic interactions between axions and magnetic monopoles. Based on the quantum electromagnetodynamics, a generic low-energy axion-photon effective field theory was built by introducing two four-potentials ($A^\mu$ and $B^\mu$) to describe a photon. More anomalous axion-photon interactions and couplings ($g_{aAA}$, $g_{aBB}$ and $g_{aAB}$) arise in contrary to the ordinary axion coupling $g_{a\gamma\gamma}aF^{\mu\nu}\tilde{F}_{\mu\nu}$. As a consequence, the conventional axion Maxwell equations are further modified. We properly solve the new axion-modified Maxwell equations and obtain the axion-induced electromagnetic fields given a static electric or magnetic field. It turns out that the dominant couplings $g_{aAB}$ and $g_{aBB}$ can be probed in the presence of external magnetic field and electric field, respectively. The induced oscillating magnetic fields are always suppressed compared with the electric fields for the axions with large Compton wavelengths. This is contrary to the situation in conventional experiments searching for the oscillating magnetic fields induced by sub-$\mu$eV axions. Thus, we propose new strategies to measure the new couplings for sub-$\mu$eV axion in haloscope experiments., Comment: 18 pages, 5 figures. Version accepted for publication in JHEP

Objective: To investigate the differences in the niacin skin flushing response of adolescent depressed patients and healthy adolescents and its diagnostic value in adolescent depression. Methods: Thirty-eight cases of acute episodes of depression in unmedicated adolescents and 47 age- and sex-matched healthy controls were included as study subjects, and sociodemographic and clinical data were collected, all of which were stimulated with six concentration gradients (up to 60 mmol/L, followed by sequential 3-fold gradient dilution to a minimum of 0.25 mmol/L) of niacin solution on the forearm skin, and the skin flushing area was applied as an assessment index. Results: The total area of redness of the skin in response to niacin was significantly lower in the adolescent depression group than in the healthy adolescent group (Z=-3.36, p=0.001) and was able to distinguish the adolescent depression group from the healthy adolescent group (area under curve=0.713, sensitivity 51.1%, specificity 83.2%). Conclusions: Niacin sensitivity is reduced in adolescent depressed patients, and the niacin skin flush response has potential clinical value as a diagnostic biomarker for adolescent depression.

ObjectiveThe present study aimed to predict myocardial ischemia in coronary heart disease (CHD) patients based on the radiologic features of coronary computed tomography angiography (CCTA) combined with clinical factors.MethodsThe imaging and clinical data of 110 patients who underwent CCTA scan before DSA or FFR examination in Changzhou Second People’s Hospital, Nanjing Medical University (90 patients), and The First Affiliated Hospital of Soochow University (20 patients) from March 2018 to January 2022 were retrospectively analyzed. According to the digital subtraction angiography (DSA) and fractional flow reserve (FFR) results, all patients were assigned to myocardial ischemia (n = 58) and normal myocardial blood supply (n = 52) groups. All patients were further categorized into training (n = 64) and internal validation (n = 26) sets at a ratio of 7:3, and the patients from second site were used as external validation. Clinical indicators of patients were collected, the left ventricular myocardium were segmented from CCTA images using CQK software, and the radiomics features were extracted using pyradiomics software. Independent prediction models and combined prediction models were established. The predictive performance of the model was assessed by calibration curve analysis, receiver operating characteristic (ROC) curve and decision curve analysis.ResultsThe combined model consisted of one important clinical factor and eight selected radiomic features. The area under the ROC curve (AUC) of radiomic model was 0.826 in training set, and 0.744 in the internal validation set. For the combined model, the AUCs were 0.873, 0.810, 0.800 in the training, internal validation, and external validation sets, respectively. The calibration curves demonstrated that the probability of myocardial ischemia predicted by the combined model was in good agreement with the observed values in both training and validation sets. The decision curve was within the threshold range of 0.1–1, and the clinical value of nomogram was higher than that of clinical model.ConclusionThe radiomic characteristics of CCTA combined with clinical factors have a good clinical value in predicting myocardial ischemia in CHD patients.

The so-called Witten effect implies the existence of electromagnetic interactions between axion and magnetic monopole due to the axion-photon coupling. A sound quantization in the presence of magnetic monopoles, called quantum electromagnetodynamics (QEMD), was utilized to construct a more generic axion-photon Lagrangian in the low-energy axion effective field theory. This generic axion-photon Lagrangian introduces the interactions between axion and two four-potentials, and leads to new axion-modified Maxwell equations. The interface haloscopes place an interface between two electromagnetic media with different properties and are desirable to search for high-mass axions $m_a\gtrsim \mathcal{O}(10)~\mu{\rm eV}$. In this work, for the generic axion-photon couplings built under QEMD, we perform comprehensive calculations of the axion-induced propagating waves and energy flux densities in different interface setups. We also obtain the sensitivity to new axion-photon couplings for high-mass axions., Comment: 16 pages, 1 figure

Additional file 1.

The pharmacokinetics (PK) of several drugs including antimicrobials might be highly altered during extracorporeal membrane oxygenation (ECMO) therapy. We present the change of voriconazole (VRC) PK during ECMO in a critically ill patient who received intravenous VRC at a maintenance dose of 200 mg every 12 h for empirical antifungal therapy. Two PK profiles were drawn before and after the initiation of ECMO therapy. Though the trough levels (both

STUDY OBJECTIVES This study aimed to establish a population pharmacokinetic (PPK) model of intravenous voriconazole (VRC) in critically ill patients with liver dysfunction and to explore the optimal dosing strategies in specific clinical scenarios for invasive fungal infections (IFIs) caused by common Aspergillus and Candida species. DESIGN Prospective pharmacokinetics study. SETTING The intensive care unit in a tertiary-care medical center. PATIENTS A total of 297 plasma VRC concentrations from 26 critically ill patients with liver dysfunction were included in the PPK analysis. METHODS Model-based simulations with therapeutic range of 2-6 mg/L as the plasma trough concentration (Cmin ) target and the free area under the concentration-time curve from 0 to 24 h (ƒAUC24 ) divided by the minimum inhibitory concentration (MIC) (ie, ƒAUC24 /MIC) ≥25 as the effective target were performed to optimize VRC dosing regimens for Child-Pugh class A and B (CP-A/B) and Child-Pugh class C (CP-C) patients. RESULTS A two-compartment model with first-order elimination adequately described the data. Significant covariates in the final model were body weight on both central and peripheral distribution volume and Child-Pugh class on clearance. Intravenous VRC loading dose of 5 mg/kg every 12 h (q12h) for the first day was adequate for CP-A/B and CP-C patients to attain the Cmin target at 24 h. The maintenance dose regimens of 100 mg q12h or 200 mg q24h for CP-A/B patients and 50 mg q12h or 100 mg q24h for CP-C patients could obtain the probability of effective target attainment of >90% at an MIC ≤0.5 mg/L and achieve the cumulative fraction of response of >90% against C. albicans, C. parapsilosis, C. glabrata, C. krusei, A. fumigatus, and A. flavus. Additionally, the daily VRC doses could be increased by 50 mg for CP-A/B and CP-C patients at an MIC of 1 mg/L, with plasma Cmin monitored closely to avoid serious adverse events. It is recommended that an appropriate alternative antifungal agent or a combination therapy could be adopted when an MIC ≥2 mg/L is reported, or when the infection is caused by C. tropicalis but the MIC value is not available. CONCLUSIONS For critically ill patients with liver dysfunction, the loading dose of intravenous VRC should be reduced to 5 mg/kg q12h. Additionally, based on the types of fungal pathogens and their susceptibility to VRC, the adjusted maintenance dose regimens with lower doses or longer dosing intervals should be considered for CP-A/B and CP-C patients.

In order to effectively deal with the security threats of home and personal Internet of Things(IoT) bot nets,especially for the objective problem of insufficient resources for anomaly detection in the home environment,a kernel density estimation-based lightweight IoT anomaly traffic detection (KDE-LIATD) method is proposed.Firstly,the KDE-LIATD method uses a Gaussian kernel density estimation method to estimate the probability density function and corresponding probability density of each dimension feature value of thenormal samples in the training set.Then,a kernel density estimation-based feature selection algorithm (KDE-FS) is proposed to obtain features that contribute significantly to anomaly detection,thereby reducing the feature dimension while improving the accuracy of anomaly detection.Finally,the cubic spline interpolation method is used to calculate the anomaly evaluation value of the test sample and perform anomaly detection.This strategy greatly reduces the computational overhead and storage overhead required to calculate the anomaly evaluation value of the test sample using the kernel density estimation method.Simulation experiment results show that the KDE-LIATD method has strong robustness and strong compatibility for anomaly traffic detection of heterogeneous IoT devices,and can effectively detect abnormal traffic in home and personal IoT bot nets.

Background::Glucose control is an important aspect in managing critically ill patients. The goal of this study was to compare the effects of sequential feeding (SF) and continuous feeding (CF) on the blood glucose of critically ill patients.Methods::A non-inferiority randomized controlled trial was adopted in this study. A total of 62 patients who were fed enteral nutritional suspension through gastric tubes were enrolled. After achieving 80% of the nutrition target calories (25 kcal·kg -1·day -1) through CF, the patients were then randomly assigned into SF and CF groups. In the SF group, the feeding/fasting time was reasonably determined according to the circadian rhythm of the human body as laid out in traditional Chinese medicine theory. The total daily dosage of the enteral nutritional suspension was equally distributed among three time periods of 7 to 9 o’clock, 11 to 13 o’clock, and 17 to 19 o’clock. The enteral nutritional suspension in each time period was pumped at a uniform rate within 2 h by an enteral feeding pump. In the CF group, patients received CF at a constant velocity by an enteral feeding pump throughout the study. Blood glucose values at five points (6:00/11:00/15:00/21:00/1:00) were monitored and recorded for seven consecutive days after randomization. Enteral feeding intolerance was also recorded. Non-inferiority testing was adopted in this study, the chi-square test or Fisher test was used for qualitative data, and the Mann-Whitney U test was used for quantitative data to determine differences between groups. In particular, a repeated measure one-way analysis of variance was used to identify whether changes in glucose value variables across the time points were different between the two groups. Results::There were no significant demographic or physiological differences between the SF and CF groups ( P > 0.050). The average glucose level in SF was not higher than that in CF (8.8 [7.3-10.3] vs. 10.7 [9.1-12.1] mmol/L, Z = -2.079, P for non-inferiority = 0.019). Hyperglycemia incidence of each patient was more common in the CF group than that in the SF group (38.4 [19.1-63.7]% vs. 11.8 [3.0-36.7]%, Z = -2.213, P = 0.027). Hypoglycemia was not found in either group. Moreover, there was no significant difference during the 7 days in the incidence of feeding intolerance ( P > 0.050). Conclusions::In this non-inferiority study, the average blood glucose in SF was not inferior to that in CF. The feeding intolerance in SF was similar to that in CF. SF may be as safe as CF for critically ill patients.Trial Registration:ClinicalTrials.gov, NCT03439618; https://clinicaltrials.gov/ct2/show/record/NCT03439618

A family of novel multi-center ionic liquids (ILs) were designed for the target of efficient transformation of CO2 into cyclic carbonates. The multi-center structures could expose more active sites than single-center ones in cycloaddition reaction. Among these ILs, with the chain-regulation strategy, the multi-center hexabutylimidazole bromobenzene (BIM-6) exhibited the performance with 95 % propylene epoxide (PO) conversion and 99 % selectivity (130 degrees C, 2.5 MPa, ILs amount 0.15 mol%, 1 h). Moreover, the multi-center ILs showed good reusability after 5 cycles and extensive suitability for different terminal epoxides. Density functional theory (DFT) and noncovalent interactions (NCI) illustrated that hydrogen-bond formed between the multi-center ILs and epoxides, together with hindrance of side chain lengths setting bromide free, synergistically activated reactants. Thus, the possible mechanism based on hydrogen-bond induction and hindrance regulation was proposed. This study provides a new approach for development of highly efficient ILs in the green chemical process.

Improving the stability of sensitive catalytic systems is an emerging research topic in the catalysis field. However, the current design of heterogeneous catalysts mainly improves their catalytic performance. This paper presents a single-atom catalyst (SAC) strategy to improve the cobalt-catalysed fluorination of acyl chlorides. A stable Co-F intermediate can be formed through the oxidative fluorination of Co

The performance of coal mine gas extraction borehole sealing material plays an essential role in the efficiency of gas extraction. Microcapsule technology was proposed to delay the expansion time of sealing materials to address the ineffective expansion of cement-based sealing materials in hydration. Based on conventional cement-based sealing materials, delayed-expansion microcapsules were prepared by phase separation with ethyl cellulose (EC) as the capsule wall material and montmorillonite (MMT) as the core material. A single-factor experiment showed that the delayed-expansion microcapsule had the best comprehensive effect when the EC content was 3%, the stirring rate was 400 rpm, the MMT content was 3.5%, and the core-wall ratio (CWR) was 10:3. Second, the slow release effect of the cement samples under the action of microcapsules is remarkable through physical tests. In addition, the triaxial compression test results show better mechanical properties of the delayed-expansion sealing materials. Then, nuclear magnetic resonance scanning was applied to the coal samples injected with different sealing materials. It was found that the proportions of macropores and mesopores in the total pore volume in the coal sample at 50 and 100 mm from the borehole wall decreased by 12.28 and 11.82%, respectively, indicating that the delayed-expansion sealing material has a better sealing effect.

Soybean red crown root rot (RCR), caused by the soil-borne fungal pathogen, Calonectria ilicicola, is the most destructive disease affecting soybean production in Japan. To date, no resistant cultivars or effective fungicides have been developed to control this disease. In this study, we evaluated 13 bacterial strains to determine their efficacy in controlling C. ilicicola. We first investigated whether the volatile organic compounds (VOCs) emitted by the bacterial strains exhibited any antifungal activity against C. ilicicola using the double-plate chamber method. The results showed that VOCs from three Pseudomonas bacterial strains, OFT2 (Pseudomonas sp.), OFT5 (Pseudomonas sp.), and Cab57 (Pseudomonas protegens), exhibited strong inhibitory activity against C. ilicicola mycelial growth. Some antifungal activity was also observed in the culture supernatants of these Pseudomonas strains. Greenhouse soil inoculation tests showed that application of OFT2, OFT5, and Cab57 cultures around soybean seeds after seed sowing significantly reduced the severity of RCR, as shown by up to 40% reduction in C. ilicicola fungal growth in the roots and 180–200% increase in shoot and root fresh weights compared to the water control. Our results suggest that OFT2, Cab57, and OFT5 produce potent antifungal compounds against C. ilicicola, thereby showing considerable potential for the biological control of C. ilicicola during soybean production.

Aim: The purpose of this study was to investigate the functions of exosomal miR-150 derived from bone marrow mesenchymal stem cells in osteonecrosis of the femoral head (ONFH). Materials & methods: Cell viability and apoptosis were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry. Alizarin red staining was performed to detect calcium deposits. A rat model was established to assess the effects of exosomal miR-150 on ONFH in vivo. Results: Exosomes or exosomal miR-150 derived from bone marrow mesenchymal stem cells inhibited TNF-α-induced osteoblast apoptosis and promoted osteogenic differentiation and autophagy. Exosomal miR-150 suppressed apoptosis and induced autophagy in TNF-α-treated osteoblasts by regulating the GREM1/NF-κB axis. Exosomal miR-150 also improved the pathological features of ONFH in vivo. Conclusion: Exosomal miR-150 alleviates ONFH by mediating the GREM1/NF-κB axis. This study provides a potential therapeutic strategy for ONFH.

Progress in data-driven self-driving laboratories for solving materials grand challenges has accelerated with the advent of machine learning, robotics, and automation, but they are usually designed with specific materials and processes in mind. To develop the next generation of materials acceleration platforms (MAPs), we propose a unified framework to enable collaboration between MAPs, leveraging on object-oriented programming principles using research groups around theworldthatwouldbeabletoeffectively evolveexperimentalworkflows.Wedemonstratetheframeworkvia three experimental case studies from disparate fields to illustrate theevolutionof,andseamlessintegrationbetween,workflows,promoting efficient resource utilization and collaboration. Moving forward, we project our framework on three other research areas that would benefit from such an evolving workflow. Through the wide adoption of our framework, we envision a collaborative, connected, global community of MAPs working together to solve scientific grand challenges. Agency for Science, Technology and Research (A*STAR) National Research Foundation (NRF) Submitted/Accepted version We acknowledge funding from Accelerated Materials Development for Manufacturing Program A1898b0043 at A*STAR via the AME Programmatic Fund by the Agency for Science, Technology and Research. K.H. also acknowledges funding from the NRF Fellowship NRF-NRFF13-2021- 0011.

Rho GTPases and Hippo kinases are key regulators of cardiomyoblast differentiation. However, how these signaling axes are coordinated spatiotemporally remains unclear. Here, the central and multifaceted roles of the BCH domain containing protein, BNIP-2, in orchestrating the expression of two key cardiac genes (cardiac troponin T [cTnT] and cardiac myosin light chain [Myl2]) in H9c2 and human embryonic stem cell-derived cardiomyocytes are delineated. This study shows that BNIP-2 mRNA and protein expression increase with the onset of cTnT and Myl2 and promote the alignment of H9c2 cardiomyocytes. Mechanistically, BNIP-2 is required for the inactivation of YAP through YAP phosphorylation and its cytosolic retention. Turbo-ID proximity labeling corroborated by super-resolution analyses and biochemical pulldown data reveals a scaffolding role of BNIP-2 for LATS1 to phosphorylate and inactivate YAP in a process that requires BNIP-2 activation of cellular contractility. The findings identify BNIP-2 as a pivotal signaling scaffold that spatiotemporally integrates RhoA/Myosin II and LATS1/YAP mechanotransduction signaling to drive cardiomyoblast differentiation, by switching the genetic programming from YAP-dependent growth to YAP-silenced differentiation. These findings offer insights into the importance of scaffolding proteins in bridging the gap between mechanical and biochemical signals in cell growth and differentiation and the prospects in translational applications.

The electrochemiluminescence and resonance energy transfer (ECL-RET) method was adopted to detect miRNAs, in which the two-dimensional Ti

Soybean red crown rot (RCR) caused by Calonectria ilicicola is a serious soil-borne disease affecting soybean production and quality. The current visual necrosis-based method for the measurement of RCR severity is prone to subjectivity as well as time consuming and laborious as it requires digging out and washing the roots to remove adhering soil prior to the visual scoring. Using cultivar Enrei, we show that, upon C. ilicicola infection, relative fresh weights (RFW; fresh weights relative to non-inoculated control plants) showed a significant negative correlation with visual RCR severity in apical shoot (trifoliate and above, R2 = 0.96), shoot (unifoliate and above, R2 = 0.82) and roots (R2 = 0.89). Furthermore, apical shoot RFW efficiently correlated with varying levels of C. ilicicola resistance in two test sets containing 37 soybean cultivars and three wild soybean accessions, exhibiting a significant correlation with visual severity (R2 = 0.72 and 0.79, p < 0.01). Taken together, our results suggest that RFW can serve as an index of soybean RCR severity, providing a simple, rapid, consistent, and cost-effective method for evaluating C. ilicicola resistance in soybeans.

Herein, a label-free photoelectrochemical immunosensor based on a g-C3N4/CdSe nanocomposite was established and applied to detect carcinoembryonic antigen (CEA). The prepared nanocomposite materials were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet-visible absorption spectroscopy (UV-vis), X-ray photoelectron spectroscopy (XPS), fourier transform infrared spectrometer (FT-IR) and photoluminescence spectroscopy (PL). The results indicate that g-C3N4/CdSe nanocomposite materials were successfully synthesized. In a typical assembly process, the immunosensor was constructed by modifying a fluorine-doped tin oxide (FTO) electrode with poly dimethyl diallyl ammonium chloride (PDDA), the g-C3N4/CdSe nanocomposite, the anti-carcinoembryonic antigen antibody (Ab) and the blocking agent bovine serum albumin (BSA) successively. In the presence of CEA, the photocurrent signal of the prepared immunosensor decreased significantly. Accordingly, under the optimal conditions, a label-free photoelectrochemical immunosensor was established, and it exhibited excellent selectivity and repeatability for CEA detection. The detection limit was 0.21 ng mL-1, and the range was 10 ng mL-1-100 μg mL-1. Simultaneously, the immunosensor also provides a likely sensing device for detecting other protein targets, which is of great significance for early clinical diagnosis.

Regulatory mechanisms of microRNAs (miRNAs) in the development of intestinal sepsis are unclear. This study investigated the role of rno-miR-146b-5p in sepsis-induced intestinal injury. A rat sepsis model was created using the cecal ligation and puncture method. The expression profiles of miRNA and mRNA in sepsis rats were examined using miRNA and mRNA sequencing; rno-miR-146b was selected for further investigation. The mimics and inhibitors of rno-miR-146b-5p were transfected into IEC-6 cells and then with or without lipopolysaccharide (LPS) treatment, and the expressions of Kruppel-like factor 4 (Klf4) and Cyclin D2 (Ccnd2) were assessed by quantitative real-time transcriptase-polymerase chain reaction (qRT-PCR) and western blotting. Next, cell counting kit-8 assay was used to detect cell viability, and scratch wound healing assay was used to assess cell migration. In sepsis rat model, crypt cell proliferation was inhibited and crypt cell apoptosis was increased. Compared with the sham control, results of miRNA and mRNA sequencing showed that there were 17 miRNAs and 1617 mRNAs that were upregulated and 123 miRNAs and 1917 mRNAs that were downregulated in the sepsis model group. The network diagrams and qRT-PCR validation indicated that rno-miR-146b-5p may inhibit the expression of Klf4. By adjusting the expression of rno-miR-146b-5p in IEC-6 cells with or without LPS treatment, we found that increased expression of rno-miR-146b-5p inhibited cell proliferation and migration and inhibited the expression of Ccnd2. rno-miR-146b-5p may play a vital role in the development of sepsis intestinal injury through targeting Klf4 expression and affecting promoter activity of Ccnd2.

It has been shown that normothermic machine perfusion (NMP), a novel preservation method, is able to assess and resuscitate liver grafts with risk factors. However, there is no consistent criteria for the assessment of liver grafts with NMP. Ischemia-free liver transplantation (IFLT) includes innovative surgical techniques and NMP, which can protect liver grafts from ischemia throughout organ procurement, preservation, and implantation. In our center, 28 human livers from donation after brain death donors were subjected to IFLT between July 2017 and October 2018. The correlation between posttransplant liver function tests with the perfusion parameters, blood gas analysis of perfusate, and bile biochemistry were analyzed. During the preservation phase, the vascular flow was stable, and the lactate level decreased rapidly. The transaminase release in the perfusate was low but stable, whereas the glucose level remained high. The perfusate lactate and aspartate aminotransferase (AST) levels at 1 hour of perfusion were correlated with the posttransplant peak AST level. There were negative correlations between the portal vein and hepatic artery flows at the end of perfusion and the peak transaminase levels within 7 days after transplantation. In conclusion, during IFLT, NMP is able to bridge the liver grafts from donors to recipients and can allow the assessment of liver function by perfusion characteristics.

Whether external root resorption is associated with hypoxia in the periodontal ligaments of teeth with severe periodontitis remains unclear. Hypoxia inducible factor-1α (HIF-1α) expression and external resorption sites in the periodontal ligaments of these teeth were observed to elaborate upon the relationship between hypoxia and external root resorption in severe periodontitis. Histological analysis was performed to observe external root resorption. The expressions of HIF-1α and Nuclear factor-activated T cells c1 (NFATc1) in the periodontal ligaments were detected by immunofluorescence, western blotting and real-time PCR. Bone marrow macrophages (BMMs) were stimulated by Porphyromonas gingivalis lipopolysaccharide (Pg.LPS) and cultured under hypoxia in vitro. High levels of HIF-1α and NFATc1 were detected in severe periodontitis. HIF-1α positive-cells were observed in the external resorption sites. Hypoxia promoted Pg.LPS-stimulated osteoclastogenesis of BMMs and bone resorption by the NFATc1 pathway. Increased HIF-1α in severe periodontitis are associated with external root resorption by the NFATc1 pathway.

Camellia rostrata S.X.Yang & S.F.Chai is described and illustrated as a new species from Guangxi, China. It is morphologically closest to C. debaoensis R.C.Hu & Y.Q.Liufu, but can be easily distinguished by its abaxially glabrous leaf surface, 1.0−1.5(−2.0) cm long pedicel, powdery puberulent inner surface of bracteoles, sepals, and petals, sparsely pubescent inner filaments base, and triangle globose or ellipsoid capsule with pointed beak. The conservation status of this new species was assessed as Critically Endangered (CR).

To solve the problem of unstable sealing in the sealing section of a gassy, soft coal seam, a seal reinforcement material for gas extraction boreholes was developed, which was mainly made of ordinary Portland cement and blended with additives such as aluminium powder, quicklime, and gypsum. Firstly, in order to obtain the necessary expansion and compressive strength of reinforcement material, key factors affecting the material properties were determined. Key factors affecting the expansion properties and compressive strength of reinforcing materials were investigated by a single-factor test. Moreover, according to the central combination (Box–Behnken) experimental principle and response surface analysis (RSA), the interactions of various factors on the expansion and compressive strength were determined, and the optimal experimental conditions were acquired. The experimental results indicated that the optimum ratio of the material was 2% for gypsum and 0.52% for aluminium powder and quicklime at the experimental temperature of 20°C, and the ratio of water to material was 0.6. Finally, in the N1103 working face of No. 3 coal seam of Yuwu coal mine, Luan Group, China, the sealing property of the reinforcement material was validated, and the problem of hole collapse at the borehole orifice was solved (resulting in a gas concentration 2.48 times that measured before borehole reinforcement), and the gas drainage effect was enhanced.

Objectives: To characterize the pharmacokinetics (PK) of intravenous voriconazole (VRC) in critically ill patients with liver dysfunction. Methods: Patients with liver dysfunction in the intensive care unit (ICU) were included prospectively. The Child–Pugh score was used to categorize the degree of liver dysfunction. The initial intravenous VRC dosing regimen comprised a loading dose of 300 mg every 12 h for the first 24 h, followed by 200 mg every 12 h. The first PK curves (PK curve 1) were drawn within one dosing interval of the first dose for 17 patients; the second PK curves (PK curve 2) were drawn within one dosing interval after a minimum of seven doses for 12 patients. PK parameters were estimated by non-compartmental analysis. Results: There were good correlations between the area under the curve (AUC0–12) of PK curve 2 and the corresponding trough concentration (C0) and peak concentration (Cmax) (r2 = 0.951 and 0.963, respectively; both p < 0.001). The median half-life (t1/2) and clearance (CL) of patients in Child–Pugh class A (n = 3), B (n = 5), and C (n = 4) of PK curve 2 were 24.4 h and 3.31 l/h, 29.1 h and 2.54 l/h, and 60.7 h and 2.04 l/h, respectively. In the different Child–Pugh classes, the CL (median) of PK curve 2 were all lower than those of PK curve 1. The apparent steady-state volume of distribution (Vss) of PK curve 1 was positively correlated with actual body weight (r2 = 0.450, p = 0.004). The median first C0 of 17 patients determined on day 5 was 5.27 (2.61) μg/ml, and 29.4% of C0 exceeded the upper limit of the therapeutic window (2–6 μg/ml). Conclusions: The CL of VRC decreased with increasing severity of liver dysfunction according to the Child–Pugh classification, along with an increased t1/2, which resulted in high plasma exposure of VRC. Adjusted dosing regimens of intravenous VRC should be established based on Child–Pugh classes for these ICU patients, and plasma concentrations should be monitored closely to avoid serious adverse events. Keywords: Voriconazole, Liver dysfunction, Pharmacokinetics, Child–Pugh score

In this work, a two-dimensional (2D) MOF sheet with electrochemiluminescence (ECL) activity is prepared with Ti