1. Molecular characterization of OsCURT1A from upland rice in response to osmotic stress
- Author
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Nurulhikma Md Isa, Cheng Seng Tan, Bernadette Toni, Ismanizan Ismail, and Zamri Zainal
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetics ,biology ,Mutant ,Wild type ,food and beverages ,Heterologous ,Plant Science ,biology.organism_classification ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Arabidopsis ,Chlorophyll ,Coding region ,Arabidopsis thaliana ,Agronomy and Crop Science ,Gene ,010606 plant biology & botany - Abstract
CURT1 proteins in 'Arabidopsis thaliana' have been reported to be important for inducing grana curvature. Currently, we have identified transcript encoding CURT1A from 'Oryza sativa' cv. indica through RNA-seq analysis and characterised using heterologous system in Arabidopsis. The 'OsCURT1A' gene shares 80% of its amino acid sequence with Arabidopsis 'AtCURT1A'. Phylogenetic analysis revealed that the 'OsCURT1A' is also closely related to the CURT1 proteins in other choloroplast- conatining organisms. In silico analysis of OsCURT1A promoter shows that several cis-elements related to stress are present in the 5' upstream from the coding region. Under normal conditions, there were no notable changes in the phenotype and chlorophyll a;b ratio between three Arabidopsis genotypes, which were overexpressed (35S::'OsCURT1A'), T-DNA insertional mutant line ('atcurt1a'), and Wild type (Col-0). However, overexpression of 'OsCURT1A' under salinity condition demonstrate high chlorophyll a:b compared to Col-0, whereas, the lack of atcurt1a gene in the mutant line showed reduced chlorophyll a:b ratio. These results indicate that the 'OsCURT1A' might have a function as salt-stress related gene, which may indirectly regulate the chlorophyll a:b ratio. Therefore, 'OsCURT1A' can be used as a potential candidate for salinity stress tolerance in crop improvement.
- Published
- 2019