1. Comparison of the Japan Society of Clinical Chemistry reference method and CDC method for HDL and LDL cholesterol measurements using fresh sera
- Author
-
Tamio Teramoto, Hiroshi Yoshida, Shinji Yokoyama, Yuzo Kayamori, Akira Tanaka, Takashi Miida, Ikuo Inoue, Koji Kishi, Satoshi Hirayama, Masami Murakami, Kunihiro Nishimura, Masumi Ai, Hiroyuki Sumino, Hirotoshi Ohmura, Tomonori Okamura, and Masakazu Nakamura more...
- Subjects
Medicine (General) ,SRM, Standard Reference Material ,Clinical Biochemistry ,NIST, National Institute of Standards and Technology ,HDL-C, high-density lipoprotein-cholesterol ,Reference method ,chemistry.chemical_compound ,R5-920 ,AK, Abell-Kendall ,Full Length Article ,medicine ,LDL-cholesterol ,LDL-C, low-density lipoprotein-cholesterol ,apo, apolipoprotein ,QD1-999 ,Homogeneous assay ,BFr-C, bottom fraction-cholesterol ,CDC, Centers for Disease Control and Prevention ,BQ, beta-quantification ,Syx, standard deviation of the regression line ,Lipoprotein cholesterol ,Ldl cholesterol ,CD, cholesterol dehydrogenase ,Chromatography ,CHE, cholesterol esterase ,Radiological and Ultrasound Technology ,Triglyceride ,Cholesterol ,Cholesterol dehydrogenase ,TG, triglycerides ,Beta-quantification ,medicine.disease ,Disease control ,HDL-cholesterol ,LB, Liebermann-Burchard ,TC, total cholesterol ,Chemistry ,EDTA·2Na, ethylenediamine-N,N′,N′,N′-tetraacetic acid, disodium salt, dihydrate ,Dextran sulfate ,chemistry ,DM, n-dodecyl-β-maltopyranoside ,lipids (amino acids, peptides, and proteins) ,EDDA, ethylenediamine-N,N′-diacetic acid ,Dyslipidemia ,HEPES, 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid ,Automated method - Abstract
Objectives In 2009, the Japan Society of Clinical Chemistry (JSCC) recommended a reference method for the measurement of serum high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels. This automated method uses cholesterol esterase-cholesterol dehydrogenase to measure cholesterol levels in fractions obtained after ultracentrifugation and dextran sulfate/magnesium chloride precipitation. In the present study, using fresh samples, we compared the LDL-C and HDL-C levels measured using this method with those measured using the traditional Centers for Disease Control and Prevention (CDC)-beta-quantification (BQ) method. Design and methods: Using both the JSCC and CDC-BQ methods, LDL-C/HDL-C levels were measured in 47 non-diseased and 126 diseased subjects, whose triglyceride levels were lower than 11.29 mmol/L (1000 mg/dL). Results For LDL-C, the equation of the line representing the correlation between the two methods was y = 0.991x + 0.009 mmol/L; r = 0.999; and Sy/x = 0.025 mmol/L, where x is the mean LDL-C level measured using the CDC-BQ method. Similarly, for HDL-C, the equation of the line representing the correlation between the two methods was y = 0.988x + 0.041 mmol/L, r = 0.999, and Sy/x = 0.019 mmol/L, where x is the mean HDL-C level measured using the CDC-BQ method. Conclusions The JSCC method agreed with the CDC-BQ method in cases of both non-diseased and diseased subjects, including those with dyslipidemia., Highlights • Using both the JSCC and BQ methods, LDL-C/HDL-C levels were measured. • The JSCC method agrees with the BQ method. • The JSCC reference method is an accurate, simple, and automatable method. • The JSCC method is suitable for quantitative analysis of LDL-C and HDL-C levels. more...
- Published
- 2021
- Full Text
- View/download PDF