1. Pathways of Purine Ribonucleotide Catabolism in Ehrlich Ascites Tumor Cells In Vitro
- Author
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Crabtree Gw and Henderson Jf
- Subjects
Adenosine ,Guanine ,Adenylate kinase ,Guanosine ,Biology ,Models, Biological ,Cell Line ,Dephosphorylation ,chemistry.chemical_compound ,Animals ,Nucleotide ,Carcinoma, Ehrlich Tumor ,Inosine Nucleotides ,chemistry.chemical_classification ,Carbon Isotopes ,Nucleotides ,Catabolism ,Adenine ,General Medicine ,Metabolism ,Ribonucleotides ,Xanthine ,Molecular biology ,Adenosine Monophosphate ,Guanine Nucleotides ,Inosine ,Kinetics ,Biochemistry ,chemistry ,Hypoxanthines ,Xanthines ,Ribonucleosides - Abstract
Ehrlich ascites tumor cells incubated in vitro with adenine-14C catabolized up to 20% of the radioactive nucleotides which were formed, principally via dephosphorylation of inosinate and xanthylate, with small amounts of adenylate and guanylate broken down. Cells incubated with hypoxanthine-14C catabolized a much larger proportion of nucleotides, principally via dephosphorylation of inosinate and xanthylate; guanylate was also broken down. Catabolic products of guanine-14C in these cells were xanthine, which was formed directly from guanine, and guanosine which at least in part was formed via dephosphorylation of guanylate.
- Published
- 1971
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