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Additional file2. Figure S2: A Sample clustering. B Scale-free fit index for various soft-thresholding powers (β). C Mean connectivity for various soft-thresholding powers.

Additional file3. Figure S3: The estimated proportion of significant immune-related features among IMS subgroups with FDR < 0.05. The top and bottom of the box are the upper quartile (Q3) and the lower quartile (Q1) of the data, respectively. The solid black line in the box represents the median. The whiskers represent the maximum and minimum values of this group of data. The Kruskal-Wallis test was used to assess significant differences. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Additional file1. Figure S1: A-B Cumulative distribution function curve (A) and (B) delta area of immune-related genes in the cohort from TCGA.

p53 is one of the most important tumor suppressor genes involved in human carcinogenesis. Although downstream targets of p53 and their biologic functions in cancer cells have been extensively investigated, it is still far from the full understanding. Here, we demonstrate that Late Cornified Envelope Group I (LCE1) genes, which are located in the LCE gene clusters encoding multiple well-conserved stratum-corneum proteins, are novel downstream targets of p53. Exogenous p53 overexpression using an adenoviral vector system significantly enhanced the expression of LCE1 cluster genes. We also observed induction of LCE1 expressions by DNA damage, which was caused by treatment with adriamycin or UV irradiation in a wild-type p53-dependent manner. Concordantly, the induction of LCE1 by DNA damage was significantly attenuated by the knockdown of p53. Among predicted p53-binding sites within the LCE1 gene cluster, we confirmed one site to be a p53-enhancer sequence by reporter assays. Furthermore, we identified LCE1 to interact with protein arginine methyltransferase 5 (PRMT5). Knockdown of LCE1 by specific small interfering RNAs significantly increased the symmetric dimethylation of histone H3 arginine 8, a substrate of PRMT5, and overexpression of LCE1F remarkably decreased its methylation level. Our data suggest that LCE1 is a novel p53 downstream target that can be directly transactivated by p53 and is likely to have tumor suppressor functions through modulation of the PRMT5 activity.

the reform and opening up, the development of legal migrants and management of the market economy provides the institutional basis. However, human rights as one of the core human rights law, especially for economic development and urban construction has made important contributions to the migrants who were not able to get real protection. Based on this background to build a harmonious society, the law should not just manage the floating population, but should play an important role in the protection of human rights of migrants. Medical benefits significantly improve the flow of population willingness to buy health insurance, but it is has no significant impact on the flow of population health care spending. Medical benefits of the floating population health investment will have a significant positive incentive effects, but it does not improve the flow of population health investment capacity. The reason may be related to the separation of urban and rural health care system, the researchers demonstrate the robustness tests to be descriptive statistical analysis and modeling of two angles. In addition, there are gender differences in the flow of population health investment behavior. Keywords-) migrants; human rights; health