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1. Modulation of the ATM/autophagy pathway by a G-quadruplex ligand tips the balance between senescence and apoptosis in cancer cells

Author

Beauvarlet, Jennifer, Bensadoun, Paul, Darbo, Elodie, Labrunie, Gaelle, Rousseau, Benoît, Richard, Elodie, Draskovic, Irena, Londono-Vallejo, Arturo, Dupuy, Jean-William, Nath Das, Rabindra, Guédin, Aurore, Robert, Guillaume, Orange, Francois, Croce, Sabrina, Valesco, Valerie, Soubeyran, Pierre, Ryan, Kevin M, Mergny, Jean-Louis, Djavaheri-Mergny, Mojgan, Institut Bergonié [Bordeaux], UNICANCER, Actions for OnCogenesis understanding and Target Identification in ONcology (ACTION), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bordeaux Segalen - Bordeaux 2-Institut Bergonié [Bordeaux], UNICANCER-UNICANCER, Acides Nucléiques : Régulations Naturelle et Artificielle (ARNA), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Européen de Chimie et Biologie (IECB), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Bordeaux (UB), Dynamique de l'information génétique : bases fondamentales et cancer (DIG CANCER), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Sorbonne Université (SU), Sorbonne Université (SU), Institut Curie [Paris], Centre Génomique Fonctionnelle Bordeaux [Bordeaux] (CGFB), Institut Polytechnique de Bordeaux-Université de Bordeaux Ségalen [Bordeaux 2], Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), University of Glasgow, Czech Academy of Sciences [Prague] (CAS), Institut Bergonié - Département de médecine, Université Bordeaux Segalen - Bordeaux 2-Centre régional de lutte contre le cancer [CRLCC], Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Centre Commun de Mesures Imagerie Cellulaire, Université de Lille, Sciences et Technologies, Telomeres and Cancer Laboratory, Institut Curie, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service d'Anatomie Pathologique Générale, CHU Strasbourg-Hôpital de Hautepierre [Strasbourg], Plateforme de génétique moléculaire des cancers d'Aquitaine, Institut Bergonié - CRLCC Bordeaux, Régulation et dynamique des génomes, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Gestionnaire, Hal Sorbonne Université, UNICANCER-UNICANCER-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Université Nice Sophia Antipolis (1965 - 2019) (UNS)

Subjects
Male, [SDV]Life Sciences [q-bio], Apoptosis, [SDV.CAN]Life Sciences [q-bio]/Cancer, Ataxia Telangiectasia Mutated Proteins, Mice, SCID, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Ligands, Mice, Chemical Biology and Nucleic Acid Chemistry, [SDV.CAN] Life Sciences [q-bio]/Cancer, Mice, Inbred NOD, Cell Line, Tumor, Neoplasms, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Autophagy, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Animals, Humans, Cellular Senescence, ComputingMilieux_MISCELLANEOUS, Mice, Knockout, Xenograft Model Antitumor Assays, G-Quadruplexes, A549 Cells, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], DNA Damage, HeLa Cells, Signal Transduction
Abstract

International audience; G-quadruplex ligands exert their antiproliferative effects through telomere-dependent and telomere-independent mechanisms, but the inter-relationships among autophagy, cell growth arrest and cell death induced by these ligands remain largely unexplored. Here, we demonstrate that the G-quadruplex ligand 20A causes growth arrest of cancer cells in culture and in a HeLa cell xenografted mouse model. This response is associated with the induction of senescence and apoptosis. Transcriptomic analysis of 20A treated cells reveals a significant functional enrichment of biological pathways related to growth arrest, DNA damage response and the lysosomal pathway. 20A elicits global DNA damage but not telomeric damage and activates the ATM and autophagy pathways. Loss of ATM following 20A treatment inhibits both autophagy and senescence and sensitizes cells to death. Moreover, disruption of autophagy by deletion of two essential autophagy genes ATG5 and ATG7 leads to failure of CHK1 activation by 20A and subsequently increased cell death. Our results, therefore, identify the activation of ATM by 20A as a critical player in the balance between senescence and apoptosis and autophagy as one of the key mediators of such regulation. Thus, targeting the ATM/autophagy pathway might be a promising strategy to achieve the maximal anticancer effect of this compound.

Published
2019

2. Human RTEL1 stabilizes long G-overhangs allowing telomerase-dependent over-extension

Author

Porreca, Rosa M, Glousker, Galina, Awad, Aya, Matilla Fernandez, Maria I, Gibaud, Anne, Naucke, Christian, Cohen, Scott B, Bryan, Tracy M, Tzfati, Yehuda, Draskovic, Irena, Londoño-Vallejo, Arturo, Dynamique de l'information génétique : bases fondamentales et cancer (DIG CANCER), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC), and Université Pierre et Marie Curie - Paris 6 (UPMC)

Subjects
Guanine, [SDV]Life Sciences [q-bio], Telomere-Binding Proteins, DNA Helicases, Humans, Telomere Homeostasis, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Genome Integrity, Repair and Replication, Telomere, Telomerase, Shelterin Complex, ComputingMilieux_MISCELLANEOUS, Cell Line
Abstract

Telomere maintenance protects the cell against genome instability and senescence. Accelerated telomere attrition is a characteristic of premature aging syndromes including Dyskeratosis congenita (DC). Mutations in hRTEL1 are associated with a severe form of DC called Hoyeraal-Hreidarsson syndrome (HHS). HHS patients carry short telomeres and HHS cells display telomere damage. Here we investigated how hRTEL1 contributes to telomere maintenance in human primary as well as tumor cells. Transient depletion of hRTEL1 resulted in rapid telomere shortening only in the context of telomerase-positive cells with very long telomeres and high levels of telomerase. The effect of hRTEL1 on telomere length is telomerase dependent without impacting telomerase biogenesis or targeting of the enzyme to telomeres. Instead, RTEL1 depletion led to a decrease in both G-overhang content and POT1 association with telomeres with limited telomere uncapping. Strikingly, overexpression of POT1 restored telomere length but not the overhang, demonstrating that G-overhang loss is the primary defect caused by RTEL1 depletion. We propose that hRTEL1 contributes to the maintenance of long telomeres by preserving long G-overhangs, thereby facilitating POT1 binding and elongation by telomerase.

Published
2018

3. FISH-in-CHIPS: A Microfluidic Platform for Molecular Typing of Cancer Cells

Author

Perez-Toralla, Karla, Mottet, Guillaume, Tulukcuoglu-Guneri, Ezgi, Champ, Jérôme, Bidard, François-Clément, Pierga, Jean-Yves, Klijanienko, Jerzy, Draskovic, Irena, Malaquin, Laurent, Viovy, Jean-Louis, Descroix, Stéphanie, Venzac, B., Diakité, L., Herthnek, D., Cissé, I., Bockelmann, U., Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Physico-Chimie-Curie (PCC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], dissemin
Abstract

International audience; Microfluidics offer powerful tools for the control, manipulation, and analysis of cells, in particular for theassessment of cell malignancy or the study of cell subpopulations. However, implementing complex biologicalprotocols on chip remains a challenge. Sample preparation is often performed off chip using multiplemanually performed steps, and protocols usually include different dehydration and drying steps thatare not always compatible with a microfluidic format.Here, we report the implementation of a Fluorescence in situ Hybridization (FISH) protocol forthe molecular typing of cancer cells in a simple and low-cost device. The geometry of the chip allowsintegrating the sample preparation steps to efficiently assess the genomic content of individual cellsusing a minute amount of sample. The FISH protocol can be fully automated, thus enabling its use inroutine clinical practice.

Published
2017

4. The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus

Author

Gentili, Matteo, Lahaye, Xavier, Nadalin, Francesca, Nader, Guilherme P.F., Puig Lombardi, Emilia, Herve, Solène, De Silva, Nilushi, Rookhuizen, Derek, Zueva, Elina, Goudot, Christel, Maurin, Mathieu, Bochnakian, Aurore, Amigorena, Sebastian, Piel, Matthieu, Fachinetti, Daniele, Manel, Nicolas, Beauvarlet, Jennifer, Bensadoun, Paul, Darbo, Elodie, Labrunie, Gaelle, Rousseau, Benoit, Richard, Elodie, Draskovic, Irena, Dupuy, Jean-William, Nath Das, Rabindra, Guédin, Aurore, Robert, Guillaume, Orange, François, Croce, Sabrina, Valesco, Valerie, Soubeyran, Pierre, Ryan, Kevin, Mergny, Jean-Louis, Mergny, Mojgan, Borie, Raphael, Bouvry, Diane, Cottin, Vincent, Gauvain, Clement, Cazes, Aurélie, Debray, Marie-Pierre, Cadranel, Jacques, Dieudé, Philippe, Degot, Tristan, Dominique, Stéphane, Gamez, Anne Sophie, Jaillet, Madeleine, Juge, Pierre-Antoine, Londono-Vallejo, Arturo, Mailleux, Arnaud, Mal, Herve, Boileau, Catherine, Ménard, Christelle, Nunes, Hilario, Prevot, Grégoire, Quetant, Sébastien, Revy, Patrick, Traclet, Julie, Wemeau-Stervinou, Lidwine, Wislez, Marie, Kannengiesser, Caroline, Crestani, Bruno, Immunité et cancer (U932), Institut Curie-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Curie, Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut Bergonié - Département de médecine, Université Bordeaux Segalen - Bordeaux 2-Centre régional de lutte contre le cancer [CRLCC], Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Centre Commun de Mesures Imagerie Cellulaire, Université de Lille, Sciences et Technologies, Telomeres and Cancer Laboratory, Centre Génomique Fonctionnelle Bordeaux [Bordeaux] (CGFB), Institut Polytechnique de Bordeaux-Université de Bordeaux Ségalen [Bordeaux 2], Acides Nucléiques : Régulations Naturelle et Artificielle (ARNA), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service d'Anatomie Pathologique Générale, CHU Strasbourg-Hôpital de Hautepierre [Strasbourg], Plateforme de génétique moléculaire des cancers d'Aquitaine, Institut Bergonié - CRLCC Bordeaux, University of Glasgow, Régulation et dynamique des génomes, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie et Epidémiologie des Maladies Respiratoires (PHERE (UMR_S_1152 / U1152)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pneumologie [Avicenne], Hôpital Avicenne, Rétrovirus et Pathologie Comparée (RPC), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Université Paris Diderot - Paris 7 (UPD7), Service de pneumologie et réanimation [CHU Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Tenon [APHP], Service de rhumatologie [Bichat], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris]-Université Paris Diderot - Paris 7 (UPD7), Service de pneumologie, oncologie thoracique et soins intensifs respiratoires [Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Département des Maladies Respiratoires [Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Hôpital Arnaud de Villeneuve, Biologie de l'Os et du Cartilage : Régulations et Ciblages Thérapeutiques (BIOSCAR (UMR_S_1132 / U1132)), Dynamique de l'information génétique : bases fondamentales et cancer (DIG CANCER), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie-Centre National de la Recherche Scientifique (CNRS), Service de pneumologie B, Université Paris Descartes - Paris 5 (UPD5), CHU, Hôpital Larrey, Respiratory Disease, CHU Grenoble-Hôpital Michallon, Developpement Normal et Pathologique du Système Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Pneumologie et Immuno-Allergologie, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Theranoscan, Université Pierre et Marie Curie - Paris 6 (UPMC), Hôpital Bichat - Claude Bernard, Physiopathologie et Epidemiologie de l'Insuffisance Respiratoire, ANR-10-LABX-77, LABEX VRI, ANR-10-IDEX-0001-02 PSL∗, LABEX DCBIOL, ACTERIA FoundationACTERIA Foundation, Fondation Schlumberger pour l’Education et la Recherche, ECTZ36691, ANRSANRS, VIH2016126002, SidactionSidaction, DIM Biothérapies, 727408 STIMUNITY, European Research CouncilEuropean Research Council, French Ministry of Education, Research, and Technology, DCBIO 751735, Marie Skłodowska-Curie Individual Fellowship, ALTF 1298-2016, EMBO Long-term fellowship, ANR INNATENUCLEOTIDES, Fondation BMS, Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Curie [Paris], Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Bergonié [Bordeaux], UNICANCER, UNICANCER-UNICANCER, Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université-Sorbonne Université, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris]-Université Paris Diderot - Paris 7 (UPD7), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC), Service de Pneumologie et Immuno-Allergologie [CHU LIlle], Pole Cardio-vasculaire et pulmonaire [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Sorbonne Université (SU), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université d'Orléans (UO), Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]-CHU Rouen

Subjects
0301 basic medicine, Male, [SDV]Life Sciences [q-bio], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], chemistry.chemical_compound, Mice, 0302 clinical medicine, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Transfection, interferon, nuclear envelope, Compartmentalization (psychology), Nucleotidyltransferases, Cell biology, Nuclear DNA, 3. Good health, centromere, Female, Adult, Satellite DNA, Guanosine, LINE, satellite DNA, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, DNA, Satellite, General Biochemistry, Genetics and Molecular Biology, Article, Cell Line, 03 medical and health sciences, Protein Domains, Animals, Humans, dendritic cells, 030304 developmental biology, Cell Nucleus, Innate immune system, DNA, IRF3, Immunity, Innate, Mice, Inbred C57BL, 030104 developmental biology, chemistry, cGAMP, 030217 neurology & neurosurgery, HeLa Cells, cGAS, STING
Abstract

Summary Cytosolic DNA activates cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) synthase (cGAS), an innate immune sensor pivotal in anti-microbial defense, senescence, auto-immunity, and cancer. cGAS is considered to be a sequence-independent DNA sensor with limited access to nuclear DNA because of compartmentalization. However, the nuclear envelope is a dynamic barrier, and cGAS is present in the nucleus. Here, we identify determinants of nuclear cGAS localization and activation. We show that nuclear-localized cGAS synthesizes cGAMP and induces innate immune activation of dendritic cells, although cGAMP levels are 200-fold lower than following transfection with exogenous DNA. Using cGAS ChIP-seq and a GFP-cGAS knockin mouse, we find nuclear cGAS enrichment on centromeric satellite DNA, confirmed by imaging, and to a lesser extent on LINE elements. The non-enzymatic N-terminal domain of cGAS determines nucleo-cytoplasmic localization, enrichment on centromeres, and activation of nuclear-localized cGAS. These results reveal a preferential functional association of nuclear cGAS with centromeres., Graphical Abstract, Highlights • Nuclear-localized cGAS activates a cellular innate immune response • Nuclear cGAS is 200-fold less active toward self-DNA than exogenous cytosolic DNA • Nuclear cGAS is enriched on centromeric satellite and LINE DNA repeats • The non-enzymatic N-terminal of cGAS determines nuclear localization and activity, cGAS is a well-established innate immune sensor of cytosolic DNA, but its presence in the nucleus is poorly understood. Gentili et al. find that nuclear cGAS is active and enriched on centromeres and LINE DNA repeats. Nuclear-cytoplasmic distribution, centromere association, and nuclear activation are determined by its non-enzymatic N-terminal domain.

Published
2019

5. An online health community for aneurysmal subarachnoid hemorrhage patients: a pilot study

Author

Boogaarts, Hieronymus, van Nuenen-Platvoet, Willemijn, van den Abbeele, Leonie, Petersen, Harriette, Draskovic, Irena, de Vries, Joost, Westert, Gert, Grotenhuis, J Andre, and Bartels, Ronald

Subjects
Original Paper, Subarachnoid hemorrhage, subarachnoid hemorrhage, business.industry, Online health communities, Computer applications to medicine. Medical informatics, Other Research Radboud Institute for Health Sciences [Radboudumc 0], R858-859.7, Usability, General Medicine, online community, medicine.disease, Online community, Patient advocacy, Chronic disorders, Likert scale, Reconstructive and regenerative medicine Radboud Institute for Health Sciences [Radboudumc 10], Quality of life (healthcare), quality of care, medicine, Medicine, Medical emergency, business
Abstract

Contains fulltext : 139048.pdf (Publisher’s version ) (Open Access) BACKGROUND: Aneurysmal subarachnoid hemorrhage (aSAH) is a condition affecting relatively young patients and has high rates of morbidity and mortality. Online health communities have emerged to fill the void for patient advocacy and information, allowing individuals with shared experiences and chronic disorders to connect. OBJECTIVE: We have developed an online health community for aSAH patients, and this pilot study was conducted to evaluate it from a patient's perspective. METHODS: We implemented an online, members-only, health community (MijnSAB, translation: MySAH) in addition to the usual aSAH care at Radboudumc, Nijmegen, the Netherlands. A questionnaire that was sent to consecutive aSAH patients was used to evaluate the usability and utility of MySAH. Answers were provided using a 5-point Likert scale. There was also one open-ended question asking about what was missing from the MySAH tool. RESULTS: In total, 66 consecutive patients with aneurysmal subarachnoid hemorrhage were informed about the online health community. Of 64 potential MySAH users, 26 patients gained access to MySAH, 20 of whom were willing to participate in the evaluation. Those who used the community were younger (P=.03) and in a better condition at discharge (P=.03). The patients were positive about MySAH's contribution to the quality of their care, but not to their quality of life. Most patients (18/20, 90%) reported that they would recommend the community to others in their position. Open suggestions on how to improve the tool included more frequent blogs, including by a rehabilitation specialist. CONCLUSIONS: This pilot study showed that the online health community, MySAH, has a beneficial effect on the aftercare of patients suffering from aSAH because it gives easy access to relevant information provided by peers or caregivers. Due to the variable clinical outcomes after aSAH, the tool will mainly be useful for a select group of patients (with a better clinical outcome).

Published
2014

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