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Objective: Atherosclerosis (AS) is a chronic inflammatory disease with high mortality. miRNAs perform a vital role in its development. This study aimed to discuss the effect of miR-145 in AS occurrence and development. Methods: The effects of miR-145 mimics and inhibitors on IL-6, IL-1β and TNF-α expressions were assessed by qRT-PCR and ELISA. CCK-8 was applied to examine the influence of miR-145 on macrophage proliferation. The influence of miR-145 on the QKI transcriptional activity was analyzed using luciferase reporter gene assy. Results: Overexpression of miR-145 could enhance the expression of IL-6, IL-1β, and TNF-α. Down-regulation of miR-145 could inhibit the proliferation of macrophages and the expression level of inflammatory cytokines. The effect of miR-145 inhibitor on the expression of inflammatory factors was partially reversed by interfering with the transcription of QKI with siRNA. Conclusion: miR-145 regulates the inflammatory response induced by macrophage activation through targeting QKI. It provides a means for AS targeted therapy.

BOG (Boil off Gas) compressor deals with the gas evaporated from the LNG storage tank. Its working temperature is lower than -120℃. There are two types of starting process for BOG compressor: Direct Starting and No-load Starting. During the starting process, the temperature on cylinder will change rapidly and result in additional thermal stress to some parts on the cylinder, especially to the bolted joints on the cylinder head. In this paper, transient Finite Element thermal analysis is proposed on the cylinder with some improvement of the boundary condition settings, such as the consideration of the ice increase on the cylinder wall. Then, the theoretical and transient FE analysis are proposed subject to the bolted joints of the cylinder head in two starting process. Result shows that the maximum temperature difference on the cylinder is 81.5℃ during direct starting; while it could decrease to 60.9℃in No-Load Starting. Along the bolted joints in the cylinder head, the maximum temperature difference will be up to 52℃ in direct starting and 45℃ in No-Load Starting. The preload increases rapidly over 60% and its mean tensile stress on the bolt is near to the yield strength. Besides, the preload are distributed unevenly during the starting process. The maximum uneven rate is 20% in Direct Starting and 15% in No-Load Starting. It shows that No-Load Starting could offer a more stable starting process. Finally, based on the theoretical analysis, a preload adjustment method is proposed to ensure the safety and validity of bolted joints on BOG compressor.

During the proceeding of problem-solving there usually will be contradiction and confliction among agents because of the single agent's capability and knowledge & the MAS's goal and resource, the agents will coordinate, consult and standardize their actions through expressing their desire intension each other or carrying on a discussion, and these are all for completing the common goal. Cooperation is an important pattern of the interaction among agents. The agents in multi-agent system (MAS) need communication and cooperation for distributed problem-solving. In this paper, a detailed discussion about communication patterns and mechanisms among agents in MAS is made, and a distributed communication structural frame on the basis of CORBA is put off; the cooperation and consultation system; studies and researches the cooperation process under a kind of cooperation mechanism is recount.