1. Antibiotic resistance genes in treated wastewater and in the receiving water bodies: A pan-European survey of urban settings
- Author
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Cacace, Damiano, Fatta-Kassinos, D., Manaia, Celia M., Cytryn, E., Kreuzinger, Norbert, Rizzo, L., Karaolia, Popi, Schwartz, Thomas, Alexander, Johannes, Merlin, Christophe, Garelick, H., Schmitt, H., Schwermer, Carsten U., Meric, Sureyya, Ozkal, Can Burak, Pons, Marie Noelle, Kneis, D., Berendonk, Thomas U., One Health Microbieel, dIRAS RA-I&I I&I, Veritati - Repositório Institucional da Universidade Católica Portuguesa, Institut für Hydrobiologie, Technische Universität Dresden = Dresden University of Technology (TU Dresden), University of Cyprus [Nicosia], Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Universidade Católica Portuguesa [Porto], Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization - the Volcani Center, Vienna University of Technology (TU Wien), Università degli Studi di Salerno (UNISA), Karlsruhe Institute of Technology (KIT), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Middlesex University [London], Institute for Risk Assessment Sciences [Utrecht, The Netherlands] (IRAS), Utrecht University [Utrecht], Norwegian Institute for Water Research (NIVA), Namik Kemal University, Laboratoire Réactions et Génie des Procédés (LRGP), and Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)
- Subjects
0208 environmental biotechnology ,Dissemination ,02 engineering and technology ,Wastewater ,010501 environmental sciences ,Integron ,01 natural sciences ,Integrons ,Persistence (computer science) ,Pan european ,Antibiotic resistance genes ,Surveys and Questionnaires ,Bacterial Community ,Treatment Plants ,Waste Management and Disposal ,ComputingMilieux_MISCELLANEOUS ,Water Science and Technology ,2. Zero hunger ,biology ,Ecological Modeling ,Drug Resistance, Microbial ,Pollution ,6. Clean water ,Anti-Bacterial Agents ,3. Good health ,Europe ,qPCR ,Impact ,Sewage treatment ,Life sciences ,Environmental Engineering ,Freshwater ,Quantification ,ddc:570 ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,Effluent ,0105 earth and related environmental sciences ,Civil and Structural Engineering ,business.industry ,Hospital Effluent ,Mcr-1 ,020801 environmental engineering ,Biotechnology ,Resistome ,Genes, Bacterial ,13. Climate action ,biology.protein ,business ,Escherichia-Coli ,Removal - Abstract
There is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents from ten different European countries. In order to assess the impact on the receiving water bodies, gene abundances in the latter were also analysed. Six out of the nine ARGs analysed were detected in all effluent and river water samples. Among the quantified genes, intI1 and sul1 were the most abundant. Our results demonstrate that European WWTP contribute to the enrichment of the resistome in the receiving water bodies with the particular impact being dependent on the effluent load and local hydrological conditions. The ARGs concentrations in WWTP effluents were found to be inversely correlated to the number of implemented biological treatment steps, indicating a possible option for WWTP management. Furthermore, this study has identified bla(OXA-58) as a possible resistance gene for future studies investigating the impact of WWTPs on their receiving water. (C) 2019 The Authors. Published by Elsevier Ltd. German Federal Ministry of Education and ResearchFederal Ministry of Education & Research (BMBF) [02WU1351A]; project STARE -Stopping Antibiotic Resistance Evolution [WaterJPI/0001/2013]; project HYREKA (BMBF)Federal Ministry of Education & Research (BMBF); Working Group 5: Wastewater Reuse and Contaminants of Emerging Concern from Norman activities; NIVA's Strategic Research Initiative on Emerging Environmental Contaminants (Research Council of Norway) [208430] The study has been funded by the German Federal Ministry of Education and Research [02WU1351A]. CMM, TB, TS, DFK acknowledge the national funding agencies through the project WaterJPI/0001/2013 STARE -Stopping Antibiotic Resistance Evolution and the project HYREKA (BMBF). This work was supported by Working Group 5: Wastewater Reuse and Contaminants of Emerging Concern from Norman activities (http://www.norman-network.net/?q=node/106).CS acknowledges the financial support from NIVA's Strategic Research Initiative on Emerging Environmental Contaminants (Research Council of Norway; contract no. 208430).
- Published
- 2019