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1. Apoptotic cell death in disease-Current understanding of the NCCD 2023

Author: Vitale, Ilio, Pietrocola, Federico, Guilbaud, Emma, Aaronson, Stuart A, Abrams, John M, Adam, Dieter, Agostini, Massimiliano, Agostinis, Patrizia, Alnemri, Emad S, Altucci, Lucia, Amelio, Ivano, Andrews, David W, Aqeilan, Rami I, Arama, Eli, Baehrecke, Eric H, Balachandran, Siddharth, Bano, Daniele, Barlev, Nickolai A, Bartek, Jiri, Bazan, Nicolas G, Becker, Christoph, Bernassola, Francesca, Bertrand, Mathieu JM, Bianchi, Marco E, Blagosklonny, Mikhail V, Blander, J Magarian, Blandino, Giovanni, Blomgren, Klas, Borner, Christoph, Bortner, Carl D, Bove, Pierluigi, Boya, Patricia, Brenner, Catherine, Broz, Petr, Brunner, Thomas, Damgaard, Rune Busk, Calin, George A, Campanella, Michelangelo, Candi, Eleonora, Carbone, Michele, Carmona-Gutierrez, Didac, Cecconi, Francesco, Chan, Francis K-M, Chen, Guo-Qiang, Chen, Quan, Chen, Youhai H, Cheng, Emily H, Chipuk, Jerry E, Cidlowski, John A, Ciechanover, Aaron, Ciliberto, Gennaro, Conrad, Marcus, Cubillos-Ruiz, Juan R, Czabotar, Peter E, D'Angiolella, Vincenzo, Daugaard, Mads, Dawson, Ted M, Dawson, Valina L, De Maria, Ruggero, De Strooper, Bart, Debatin, Klaus-Michael, Deberardinis, Ralph J, Degterev, Alexei, Del Sal, Giannino, Deshmukh, Mohanish, Di Virgilio, Francesco, Diederich, Marc, Dixon, Scott J, Dynlacht, Brian D, El-Deiry, Wafik S, Elrod, John W, Engeland, Kurt, Fimia, Gian Maria, Galassi, Claudia, Ganini, Carlo, Garcia-Saez, Ana J, Garg, Abhishek D, Garrido, Carmen, Gavathiotis, Evripidis, Gerlic, Motti, Ghosh, Sourav, Green, Douglas R, Greene, Lloyd A, Gronemeyer, Hinrich, Häcker, Georg, Hajnóczky, György, Hardwick, J Marie, Haupt, Ygal, He, Sudan, Heery, David M, Hengartner, Michael O, Hetz, Claudio, Hildeman, David A, Ichijo, Hidenori, Inoue, Satoshi, Jäättelä, Marja, Janic, Ana, Joseph, Bertrand, Jost, Philipp J, and Kanneganti, Thirumala-Devi
Subjects: Mammals, Biochemistry & Molecular Biology, Cell Death, Carcinogenesis, Liver Disease, 1.1 Normal biological development and functioning, Apoptosis, Biological Sciences, Medical and Health Sciences, Good Health and Well Being, Underpinning research, Caspases, Animals, Humans, Generic health relevance, Digestive Diseases
Abstract: Apoptosis is a form of regulated cell death (RCD) that involves proteases of the caspase family. Pharmacological and genetic strategies that experimentally inhibit or delay apoptosis in mammalian systems have elucidated the key contribution of this process not only to (post-)embryonic development and adult tissue homeostasis, but also to the etiology of multiple human disorders. Consistent with this notion, while defects in the molecular machinery for apoptotic cell death impair organismal development and promote oncogenesis, the unwarranted activation of apoptosis promotes cell loss and tissue damage in the context of various neurological, cardiovascular, renal, hepatic, infectious, neoplastic and inflammatory conditions. Here, the Nomenclature Committee on Cell Death (NCCD) gathered to critically summarize anabundant pre-clinical literature mechanistically linking the core apoptotic apparatus to organismal homeostasis in the context of disease.
Published: 2023

2. Apoptotic cell death in disease—Current understanding of the NCCD 2023

Author: Vitale, Ilio, Pietrocola, Federico, Guilbaud, Emma, Aaronson, Stuart A, Abrams, John M, Adam, Dieter, Agostini, Massimiliano, Agostinis, Patrizia, Alnemri, Emad S, Altucci, Lucia, Amelio, Ivano, Andrews, David W, Aqeilan, Rami I, Arama, Eli, Baehrecke, Eric H, Balachandran, Siddharth, Bano, Daniele, Barlev, Nickolai A, Bartek, Jiri, Bazan, Nicolas G, Becker, Christoph, Bernassola, Francesca, Bertrand, Mathieu J M, Bianchi, Marco E, Blagosklonny, Mikhail V, Blander, J Magarian, Blandino, Giovanni, Blomgren, Klas, Borner, Christoph, Bortner, Carl D, Bove, Pierluigi, Boya, Patricia, Brenner, Catherine, Broz, Petr, Brunner, Thomas, Damgaard, Rune Busk, Calin, George A, Campanella, Michelangelo, Candi, Eleonora, Carbone, Michele, Carmona-Gutierrez, Didac, Cecconi, Francesco, Chan, Francis K-M, Chen, Guo-Qiang, Chen, Quan, Chen, Youhai H, Cheng, Emily H, Chipuk, Jerry E, Cidlowski, John A, Ciechanover, Aaron, Ciliberto, Gennaro, Conrad, Marcus, Cubillos-Ruiz, Juan R, Czabotar, Peter E, D'Angiolella, Vincenzo, Daugaard, Mads, Dawson, Ted M, Dawson, Valina L, De Maria, Ruggero, De Strooper, Bart, Debatin, Klaus-Michael, Deberardinis, Ralph J, Degterev, Alexei, Del Sal, Giannino, Deshmukh, Mohanish, Di Virgilio, Francesco, Diederich, Marc, Dixon, Scott J, Dynlacht, Brian D, El-Deiry, Wafik S, Elrod, John W, Engeland, Kurt, Fimia, Gian Maria, Galassi, Claudia, Ganini, Carlo, Garcia-Saez, Ana J, Garg, Abhishek D, Garrido, Carmen, Gavathiotis, Evripidis, Gerlic, Motti, Ghosh, Sourav, Green, Douglas R, Greene, Lloyd A, Gronemeyer, Hinrich, Häcker, Georg, Hajnóczky, György, Hardwick, J Marie, Haupt, Ygal, He, Sudan, Heery, David M, Hengartner, Michael O, Hetz, Claudio, Hildeman, David A, Ichijo, Hidenori, Inoue, Satoshi, Jäättelä, Marja, Janic, Ana, Joseph, Bertrand, Jost, Philipp J, Kanneganti, Thirumala-Devi, Karin, Michael, Kashkar, Hamid, Kaufmann, Thomas, Kelly, Gemma L, Kepp, Oliver, Kimchi, Adi, Kitsis, Richard N, Klionsky, Daniel J, Kluck, Ruth, Krysko, Dmitri V, Kulms, Dagmar, Kumar, Sharad, Lavandero, Sergio, Lavrik, Inna N, Lemasters, John J, Liccardi, Gianmaria, Linkermann, Andreas, Lipton, Stuart A, Lockshin, Richard A, López-Otín, Carlos, Luedde, Tom, MacFarlane, Marion, Madeo, Frank, Malorni, Walter, Manic, Gwenola, Mantovani, Roberto, Marchi, Saverio, Marine, Jean-Christophe, Martin, Seamus J, Martinou, Jean-Claude, Mastroberardino, Pier G, Medema, Jan Paul, Mehlen, Patrick, Meier, Pascal, Melino, Gerry, Melino, Sonia, Miao, Edward A, Moll, Ute M, Muñoz-Pinedo, Cristina, Murphy, Daniel J, Niklison-Chirou, Maria Victoria, Novelli, Flavia, Núñez, Gabriel, Oberst, Andrew, Ofengeim, Dimitry, Opferman, Joseph T, Oren, Moshe, Pagano, Michele, Panaretakis, Theocharis, Pasparakis, Manolis, Penninger, Josef M, Pentimalli, Francesca, Pereira, David M, Pervaiz, Shazib, Peter, Marcus E, Pinton, Paolo, Porta, Giovanni, Prehn, Jochen H M, Puthalakath, Hamsa, Rabinovich, Gabriel A, Rajalingam, Krishnaraj, Ravichandran, Kodi S, Rehm, Markus, Ricci, Jean-Ehrland, Rizzuto, Rosario, Robinson, Nirmal, Rodrigues, Cecilia M P, Rotblat, Barak, Rothlin, Carla V, Rubinsztein, David C, Rudel, Thomas, Rufini, Alessandro, Ryan, Kevin M, Sarosiek, Kristopher A, Sawa, Akira, Sayan, Emre, Schroder, Kate, Scorrano, Luca, Sesti, Federico, Shao, Feng, Shi, Yufang, Sica, Giuseppe S, Silke, John, Simon, Hans-Uwe, Sistigu, Antonella, Stephanou, Anastasis, Stockwell, Brent R, Strapazzon, Flavie, Strasser, Andreas, Sun, Liming, Sun, Erwei, Sun, Qiang, Szabadkai, Gyorgy, Tait, Stephen W G, Tang, Daolin, Tavernarakis, Nektarios, Troy, Carol M, Turk, Boris, Urbano, Nicoletta, Vandenabeele, Peter, Vanden Berghe, Tom, Vander Heiden, Matthew G, Vanderluit, Jacqueline L, Verkhratsky, Alexei, Villunger, Andreas, von Karstedt, Silvia, Voss, Anne K, Vousden, Karen H, Vucic, Domagoj, Vuri, Daniela, Wagner, Erwin F, Walczak, Henning, Wallach, David, Wang, Ruoning, Wang, Ying, Weber, Achim, Wood, Will, Yamazaki, Takahiro, Yang, Huang-Tian, Zakeri, Zahra, Zawacka-Pankau, Joanna E, Zhang, Lin, Zhang, Haibing, Zhivotovsky, Boris, Zhou, Wenzhao, Piacentini, Mauro, Kroemer, Guido, Galluzzi, Lorenzo, Vitale, Ilio, Pietrocola, Federico, Guilbaud, Emma, Aaronson, Stuart A, Kumar, Sharad, Galluzzi, Lorenzo, Associazione Italiana per la Ricerca sul Cancro, Italian Institute for Genomic Medicine, Compagnia di San Paolo, Aaronson, Stuart A., Dieter, Adam, Agostini, Massimiliano, Agostinis, Patrizia, Alnemri, Emad S., Altucci, Lucia, Amelio, Ivano, Andrews, David W., Aqeilan, Rami I., Arama, Eli, Balachandran, Siddharth, Bano, Daniele, Bartek, Jiri, Bazan, Nicolas G., Bernassola, Francesca, Bertrand, Mathieu J. M., Bianchi, Marco Emilio, Blander, J. Magarian, Blandino, Giovanni, Blomgren, Klas, Bortner, Carl D., Bove, Pierluigi, Boya, Patricia, Broz, Petr, Damgaard, Rune Busk, Calin, George A., Campanella, Michelangelo, Candi, Eleonora, Carbone, Michele, Carmona-Gutierrez, Didac, Cecconi, Francesco, Chen, Guo‑Qiang, Cheng, Emily H., Chipuk, Jerry E., Cidlowski, John A., Ciechanover, Aaron, Ciliberto, Gennaro, Conrad, Marcus, Czabotar, Peter E., D’Angiolella, Vincenzo, Daugaard, Mads, Dawson, Valina L., De Maria, Ruggero, Debatin, Klaus-Michael, Deberardinis, Ralph J., Degterev, Alexei, Del Sal, Giannino, Deshmukh, Mohanish, Di Virgilio, Francesco, Diederich, Marc, Dixon, Scott J., El-Deiry, Wafik S., Elrod, John W., Engeland, Kurt, Fimia, Gian María, Ganini, Carlo, García-Sáez, Ana J., Garg, Abhishek D., Garrido, Carmen, Gavathiotis, Evripidis, Ghosh, Sourav, Green, Douglas R., Gronemeyer, Hinrich, Häcker, Georg, Hajnóczky, György, Hardwick, J. Marie, Haupt, Ygal, He, Sudan, Heery, David M., Hengartner, Michael O., Hetz, Claudio, Hildeman, David A., Ichijo, Hidenori, Jäättelä, Marja, Janic, Ana, Joseph, Bertrand, Jost, Philipp J., Kanneganti, Thirumala-Devi, Karin, Michael, Kashkar, Hamid, Kaufmann, Thomas, Kelly, Gemma L., Kepp, Oliver, Kimchi, Adi, Klionsky, Daniel J., Kluck, Ruth, Krysko, Dmitri V., Kulms, Dagmar, Lavandero, Sergio, Lavrik, Inna N., Liccardi, Gianmaria, Linkermann, Andreas, Lipton, Stuart A., Lockshin, Richard A., López-Otín, Carlos, Luedde, Tom, MacFarlane, Marion, Madeo, Frank, Malorni, Walter, Manic, Gwenola, Marchi, Saverio, Marine, Jean-Christophe, Martin, Seamus J., Martinou, Jean-Claude, Mastroberardino, Pier G., Medema, Jan Paul, Mehlen, Patrick, Meier, Pascal, Melino, Gerry, Melino, Sonia, Miao, Edward A., Moll, Ute M., Muñoz-Pinedo, Cristina, Murphy, Daniel J., Niklison-Chirou, Maria Victoria, Novelli, Flavia, Oberst, Andrew, Ofengeim, Dimitry, Opferman, Joseph T., Oren, Moshe, Pagano, Michele, Panaretakis, Theocharis, Pasparakis, Manolis, Penninger, Josef M., Pentimalli, Francesca, Pereira, David M., Pervaiz, Shazib, Peter, Marcus E., Pinton, Paolo, Porta, Giovanni, Puthalakath, Hamsa, Rabinovich, Gabriel A., Rajalingam, Krishnaraj, Ravinchandran, Kodi S., Rehm, Markus, Ricci, Jean-Ehrland, Rizzuto, Rosario, Robinson, Nirmal, Rotblat, Barak, Rothlin, Carla V., Rubinsztein, David C., Rufini, Alessandro, Ryan, Kevin M., Sarosiek, Kristopher A., Sawa, Akira, Sayan, Emre, Schroder, Kate, Scorrano, Luca, Sesti, Federico, Shi, Yufang, Sica, Giuseppe, Silke, John, Simon, Hans-Uwe, Sistigu, Antonella, Stockwell, Brent R., Strappazzon, Flavie, Sun, Liming, Sun, Erwei, Szabadkai, G, Tait, Stephen W. G., Tang, Daolin, Tavernarakis, Nektarios, Turk, Boris, Urbano, Nicoletta, Vandenabeele, Peter, Vanden Berghe, Tom, Vander Heiden, Matthew G., Vanderluit, Jacqueline L., Verkhratsky, A., Villunger, Andreas, Von Karstedt, Silvia, Voss, Anne K., Vucic, Domagoj, Vuri, Daniela, Wagner, Erwin F., Walczak, Henning, Wallach, David, Wang, Ruoning, Weber, Achim, Yamazaki, Takahiro, Zakeri, Zahra, Zawacka-Pankau, Joanna E., Zhivotovsky, Boris, Piacentini, Mauro, Kroemer, Guido, Vitale, Ilio [0000-0002-5918-1841], Piacentini, Mauro [0000-0003-2919-1296], Kroemer, Guido [0000-0002-9334-4405], Galluzzi, Lorenzo [0000-0003-2257-8500], Apollo - University of Cambridge Repository, Abrams, John M, Adam, Dieter, Alnemri, Emad S, Andrews, David W, Aqeilan, Rami I, Baehrecke, Eric H, Barlev, Nickolai A, Bazan, Nicolas G, Becker, Christoph, Bertrand, Mathieu J M, Bianchi, Marco E, Blagosklonny, Mikhail V, Blander, J Magarian, Blomgren, Kla, Borner, Christoph, Bortner, Carl D, Brenner, Catherine, Brunner, Thoma, Calin, George A, Chan, Francis K-M, Chen, Guo-Qiang, Chen, Quan, Chen, Youhai H, Cheng, Emily H, Chipuk, Jerry E, Cidlowski, John A, Conrad, Marcu, Cubillos-Ruiz, Juan R, Czabotar, Peter E, D'Angiolella, Vincenzo, Daugaard, Mad, Dawson, Ted M, Dawson, Valina L, De Strooper, Bart, Deberardinis, Ralph J, Dixon, Scott J, Dynlacht, Brian D, El-Deiry, Wafik S, Elrod, John W, Fimia, Gian Maria, Galassi, Claudia, Garcia-Saez, Ana J, Garg, Abhishek D, Gavathiotis, Evripidi, Gerlic, Motti, Green, Douglas R, Greene, Lloyd A, Hardwick, J Marie, Heery, David M, Hengartner, Michael O, Hildeman, David A, Inoue, Satoshi, Jost, Philipp J, Kaufmann, Thoma, Kelly, Gemma L, Kitsis, Richard N, Klionsky, Daniel J, Krysko, Dmitri V, Lavrik, Inna N, Lemasters, John J, Linkermann, Andrea, Lipton, Stuart A, Lockshin, Richard A, López-Otín, Carlo, Macfarlane, Marion, Mantovani, Roberto, Martin, Seamus J, Mastroberardino, Pier G, Miao, Edward A, Moll, Ute M, Murphy, Daniel J, Núñez, Gabriel, Opferman, Joseph T, Panaretakis, Theochari, Pasparakis, Manoli, Penninger, Josef M, Pereira, David M, Peter, Marcus E, Prehn, Jochen H M, Rabinovich, Gabriel A, Ravichandran, Kodi S, Rehm, Marku, Rodrigues, Cecilia M P, Rothlin, Carla V, Rubinsztein, David C, Rudel, Thoma, Ryan, Kevin M, Sarosiek, Kristopher A, Shao, Feng, Sica, Giuseppe S, Stephanou, Anastasi, Stockwell, Brent R, Strapazzon, Flavie, Strasser, Andrea, Sun, Qiang, Szabadkai, Gyorgy, Tait, Stephen W G, Tavernarakis, Nektario, Troy, Carol M, Turk, Bori, Vander Heiden, Matthew G, Vanderluit, Jacqueline L, Verkhratsky, Alexei, Villunger, Andrea, von Karstedt, Silvia, Voss, Anne K, Vousden, Karen H, Wagner, Erwin F, Wang, Ying, Wood, Will, Yang, Huang-Tian, Zawacka-Pankau, Joanna E, Zhang, Lin, Zhang, Haibing, Zhivotovsky, Bori, and Zhou, Wenzhao
Subjects: Mammals, genetics [Caspases], Cell Death, Settore BIO/11, Carcinogenesis, Cell death, diseases, Settore BIO/12, metabolism [Mammals], Apoptosis, Cell Biology, genetic strategies, regulated cell death (RCD), SDG 3 - Good Health and Well-being, cell loss and tissue damage, Settore BIO/10 - Biochimica, Caspases, Animals, Humans, metabolism [Caspases], ddc:610, Settore BIO/10, 610 Medizin und Gesundheit, Molecular Biology, genetics [Apoptosis]
Abstract: 58 p.-5 fig.-7 box., Apoptosis is a form of regulated cell death (RCD) that involves proteases of the caspase family. Pharmacological and genetic strategies that experimentally inhibit or delay apoptosis in mammalian systems have elucidated the key contribution of this process not only to (post-)embryonic development and adult tissue homeostasis, but also to the etiology of multiple human disorders. Consistent with this notion, while defects in the molecular machinery for apoptotic cell death impair organismal development and promote oncogenesis, the unwarranted activation of apoptosis promotes cell loss and tissue damage in the context of various neurological, cardiovascular, renal, hepatic, infectious, neoplastic and inflammatory conditions. Here, the Nomenclature Committee on Cell Death (NCCD) gathered to critically summarize an abundant pre-clinical literature mechanistically linking the core apoptotic apparatus to organismal homeostasis in the context of disease., I. Vitale is and has been supported by the Associazione Italiana per la Ricerca sul Cancro (AIRC, IG 2017 #20417 and IG 2022 #27685) and by a startup grant from the Italian Institute for Genomic Medicine (Candiolo, Turin, Italy) and Compagnia di San Paolo (Torino, Italy). M. Piacentini, G. Melino, S. Melino, G. Ciliberto are supported by the Ministro dell’Università (Italy) progetto Heal Italia PE6. L. Galluzzi is/has been supported (as a PI unless otherwise indicated) by two Breakthrough Level 2 grants from the US DoD BCRP (#BC180476P1; #BC210945), by a Transformative Breast Cancer Consortium Grant from the US DoD BCRP (#W81XWH2120034, PI: Formenti), by a U54 grant from NIH/NCI (#CA274291, PI: Deasy, Formenti, Weichselbaum), by the 2019 Laura Ziskin Prize in Translational Research (#ZP-6177, PI: Formenti) from the Stand Up to Cancer (SU2C), by a Mantle Cell Lymphoma Research Initiative (MCL-RI, PI: Chen-Kiang) grant from the Leukemia and Lymphoma Society (LLS), by a Rapid Response Grant from the Functional Genomics Initiative (New York, US), by startup funds from the Dept. of Radiation Oncology at Weill Cornell Medicine (New York, US), by industrial collaborations with Lytix Biopharma (Oslo, Norway), Promontory (New York, US) and Onxeo (Paris, France), as well as by donations from Promontory (New York, US), the Luke Heller TECPR2 Foundation (Boston, US), Sotio a.s. (Prague, Czech Republic), Lytix Biopharma (Oslo, Norway), Onxeo (Paris, France), Ricerchiamo (Brescia, Italy), and Noxopharm (Chatswood, Australia). G. Kroemer is supported by the Ligue contre le Cancer (équipe labellisée); Agence National de la Recherche (ANR) – Projets blancs; AMMICa US23/CNRS UMS3655; Association pour la recherche sur le cancer (ARC); Cancéropôle Ile-de-France; European Research Council Advanced Investigator Grand “ICD-Cancer”, Fondation pour la Recherche Médicale (FRM); a donation by Elior; Equipex Onco-Pheno-Screen; European Joint Programme on Rare Diseases (EJPRD); European Research Council (ICD-Cancer), European Union Horizon 2020 Projects Oncobiome and Crimson; Fondation Carrefour; Institut National du Cancer (INCa); Institut Universitaire de France; LabEx Immuno-Oncology (ANR-18-IDEX-0001); a Cancer Research ASPIRE Award from the Mark Foundation; the RHU Immunolife; Seerave Foundation; SIRIC Stratified Oncology Cell DNA Repair and Tumor Immune Elimination (SOCRATE); and SIRIC Cancer Research and Personalized Medicine (CARPEM)
Published: 2023

3. The State-Sponsored Genocide of the Rohingya Community from a Constructivist Perspective

Author: Romero Garrido, Carmen
Subjects: Rohingya, genocide, genocidio, constructivism, constructivismo, Buddhism, budismo, General Medicine, Corte Internacional de Justicia, refugees, Islam, refugiados, International Court of Justice
Abstract: The Rohingya community has been persecuted by Myanmar authorities for years and, consequently, subjected to a process of genocide. The object of this study is the investigation of the conflict born between both communities and its religious roots: Buddhism and Islam. Afterwards, we will focus on the analysis of this historical struggle from the perspective of the Theory of Constructivism and how succeeded the construction of the antagonistic identity of “other”, the “enemy”, the “foe” and the “hostile” from within the government, the governmental institutions and the Myanmar public inside culture, legislation, and constant historical manipulation. El pueblo Rohingya ha sido objeto de persecución por las autoridades birmanas y, consecuentemente, sometido a un genocidio. El objeto de este estudio es la investigación del conflicto nacido entre dos pueblos y sus raíces religiosas: budismo e islam. Posteriormente, analizaremos este enconamiento histórico desde un punto de vista propio de la teoría constructivista y cómo se ha logrado la construcción de una identidad antagonista del “otro”, del “enemigo” y lo “hostil” por parte del estado, las instituciones gubernamentales y el pueblo birmano dentro de la cultura, la legislación y la constante manipulación histórica.
Published: 2022

4. TWINLAB: técnicas de visualización, ingeniería y ciencia de datos para el desarrollo de un gemelo digital de un laboratorio de hidroacústica, que habilite la caracterización avanzada de equipos y materiales submarinos

Author: García, Mª Ángeles, Isabel, Hernández, Felis, Iván, Ruiz, Pablo, Garrido, Carmen, Martínez, Rosa, Madrid, Eduardo, Saura, Pablo, and Errachdi, Hamid
Subjects: Gemelo digital, Materiales submarinos, Laboratorio, Caracterización avanzada, Hidroacústica, Tecnología 4.0, Aprendizaje automático, Ciencia de datos
Abstract: Este informe, elaborado por el equipo del Centro Tecnológico Naval y del Mar, tiene como finalidad ofrecer al tejido empresarial una mejora en el conocimiento del entorno que permita detectar tendencias y desarrollar estrategias adecuadas, basadas en niveles superiores de certidumbre a través de la captación y divulgación de información y conocimiento de importancia estratégica en los ámbitos social, tecnológico y económico, que incidan en la detección de nuevas oportunidades de desarrollo regional. Los contenidos de este informe están estrechamente relacionados con el desarrollo del proyecto Investigación de técnicas de visualización, ingeniería y ciencia de datos para el desarrollo de un gemelo digital de un laboratorio de hidroacústica, que habilite la caracterización avanzada de equipos y materiales submarinos. TwinLab pretende acercar distancias entre las tecnologías 4.0y el contexto de laboratorio, mediante el desarrollo de un gemelo digital de un tanque de hidroacústica que permitirá el análisis de datos e incorporará el aprendizaje automático.
Published: 2023
Full Text: View/download PDF

5. Scoping Review

Author: Cabrera-León, Andrés, Garrido, Carmen, and Gosálvez-Prados, Diana
Abstract: The objective of this review is to identify models in the evaluation of the care needs of people with chronic diseases that consider social, economic, demographic and environmental determinants of health to estimate survival, stratify risk or characterize this population.
Published: 2022
Full Text: View/download PDF

6. Author response for 'Lipidomic profiling of exosomes from colorectal cancer cells and patients reveals potential biomarkers'

Author: null Elmallah, Mohammed I. Y., null Ortega-Deballon, Pablo, null Hermite, Laure, null Pais-De-Barros, Jean-Paul, null Gobbo, Jessica, and null Garrido, Carmen
Published: 2022

7. La imagen de la mujer en el sector hotelero desde un enfoque interseccional

Author: Marín Garrido, Carmen María, Díaz Fernández, María del Carmen, Cobeña Ruiz-Lopera, María del Mar, and Universidad de Sevilla. Departamento de Administración de Empresas y Comercialización e Investigación de Mercados (Marketing)
Subjects: Cliente externo, Discriminación, Desigualdad, Cliente interno, Género, Campañas publicitarias, Imagen, Redes sociales, Interseccionalidad
Abstract: La interseccionalidad se impone hoy en día como uno de los puntos clave a la hora de elaborar políticas de igualdad. Desde este enfoque, analizaremos las posibles desigualdades existentes en dos de los ámbitos en los que interactúa la mujer en el sector hotelero, interno y externo. En esta línea, estudiaremos si el servicio ofrecido por el sector se adecua o no a las necesidades de este colectivo desde una mirada interseccional. Se analizará la imagen que se proyecta de la mujer en redes sociales y campañas publicitarias, así como su posible influencia en la demanda del servicio. Para ello, se lleva a cabo un estudio exploratoriodescriptivo, basado en la obtención de datos a partir de un cuestionario elaborado al respecto. Universidad de Sevilla. Máster en Dirección y Planificación del Turismo
Published: 2022

8. Passport for leisure? Comment on the judgment of the spanish Supreme Court 1112/2021, of September 14, which endorses the requirement of the 'Covid passport' in bars and restaurants in Galicia

Author: Delgado Garrido, Carmen
Subjects: Public health, Pasaporte Covid, Ratificación judicial, Salud pública, Fundamental rights, Covid Passport, Derechos fundamentales, Judicial ratification
Abstract: Este estudio analiza la Sentencia 1112/2021 del Tribunal Supremo español (rec. Casación 5909/2021) sobre la exhibición del “pasaporte Covid” para el acceso al ocio en Galicia. Considera la necesidad de solicitar la autorización judicial por verse afectados los derechos fundamentales a la igualdad, intimidad y protección de datos, aunque sea de forma leve o liviana. Para estas concretas actividades es una limitación proporcionada, idónea y necesaria, primando las razones de salud pública en el contexto epidemiológico actual., This study analyzes Judgment 1112/2021 of the Spanish Supreme Court (rec. Cassation 5909/2021) regarding the exhibition of the ''Covid passport'' for access to leisure in Galicia. It considers the need to request judicial authorization because the fundamental rights to equality, privacy and data protection are affected, although it is mild or light. For these specific activities, it is a proportionate, appropriate and necessary limitation, giving priority to public health reasons in the current epidemiological context.
Published: 2021

9. El nuevo marco internacional de la fiscalidad de las multinacionales

Author: Domínguez Martínez, José M., Molina Garrido, Carmen, and Universidad de Alcalá. Instituto Universitario de Análisis Económico y Social (IAES)
Subjects: Tributación multinacionales, Economics, OCDE, Economía, Multinational taxation, Sociology, Impuesto sobre sociedades, OECD, G20, Corporate taxation, G7, Sociología, BEPS
Abstract: 43 p., Este trabajo tiene como objetivo ofrecer una visión introductoria acerca del alcance y las implicaciones de la reforma del marco internacional de la fiscalidad de las multinacionales acordada en la reunión del G7 de junio de 2021 y posteriormente asumida por 132 países, así como por el G20. Inicialmente se exponen los fundamentos del sistema que ha estado vigente desde hace un siglo, así como las deficiencias puestas de manifiesto a lo largo del tiempo, acentuadas en un contexto de globalización y digitalización. Posteriormente, tras aludir a los antecedentes sobre la materia, se efectúa un repaso del desarrollo del proyecto impulsado por la OCDE para hacer frente al fenómeno BEPS, en el que se basa el referido acuerdo del G7. Los principales aspectos de éste, sustentando en los denominados Pilares Uno y Dos, son objeto de exposición y análisis. Su implementación representa un hito de gran trascendencia que dará lugar a una importante transformación en el ámbito de la fiscalidad de las multinacionales., This paper aims to provide an introductory view on the scope and implications of the reform of the international framework for multinational taxation agreed at the G7 meeting in June 2021 and subsequently assumed by 132 countries, as well as by the G20. Initially, the foundations of the system in place for a century are exposed, as well as the deficiencies revealed over time, accentuated in a context of globalization and digitization. Subsequently, after a brief background on the matter, a review is made of the development of the project promoted by the OECD to deal with the BEPS phenomenon, on which the aforementioned G7 agreement is based. The main aspects of this, based on the so-called Pillars One and Two, are the object of exposition and analysis. Its implementation represents a major milestone of great significance that will lead to an important transformation in the field of multinational taxation.
Published: 2021

10. Moderate SIRT1 overexpression protects against inflammation of brown adipose tissue

Author: Escalona-Garrido, Carmen, Vázquez Pérez, Patricia, Mera, Paula, Zagmutt, Sebastián, García-Casarrubios, Ester, Montero-Pedrazuela, Ana, Rey-Stolle, Fernanda, Guadaño-Ferraz, Ana, Rupérez, Francisco J., Serra, Dolors, Herrero, Laura, Obregón, María Jesús, Valverde, Ángela M., Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Comunidad de Madrid, Fundación Ramón Areces, Generalitat de Catalunya, Fundació La Marató de TV3, Instituto de Salud Carlos III, and Agencia Nacional de Investigación y Desarrollo (Chile)
Subjects: Inflammation, Sirtuin 1, Triiodothyronine, Insulin resistance, Brown adipose tissue, Uncoupling protein-1
Abstract: © 2020 The Author(s)., [Objective]: Metainflammation is a chronic low-grade inflammatory state induced by obesity and associated comorbidities, including peripheral insulin resistance. Brown adipose tissue (BAT), a therapeutic target against obesity, is an insulin target tissue sensitive to inflammation. Therefore, it is necessary to find strategies to protect BAT against the effects of inflammation in energy balance. In this study, we explored the impact of moderate sirtuin 1 (SIRT1) overexpression on insulin sensitivity and ß-adrenergic responses in BAT and brown adipocytes (BA) under pro-inflammatory conditions., [Methods]: The effect of inflammation on BAT functionality was studied in obese db/db mice and lean wild-type (WT) mice or mice with moderate overexpression of SIRT1 (SIRT1Tg+) injected with a low dose of bacterial lipopolysaccharide (LPS) to mimic endotoxemia. We also conducted studies on differentiated BA (BA-WT and BA-SIRT1Tg+) exposed to a macrophage-derived pro-inflammatory conditioned medium (CM) to evaluate the protection of SIRT1 overexpression in insulin signaling and glucose uptake, mitochondrial respiration, fatty acid oxidation (FAO), and norepinephrine (NE)-mediated-modulation of uncoupling protein-1 (UCP-1) expression., [Results]: BAT from the db/db mice was susceptible to metabolic inflammation manifested by the activation of pro-inflammatory signaling cascades, increased pro-inflammatory gene expression, tissue-specific insulin resistance, and reduced UCP-1 expression. Impairment of insulin and noradrenergic responses were also found in the lean WT mice upon LPS injection. In contrast, BAT from the mice with moderate overexpression of SIRT1 (SIRT1Tg+) was protected against LPS-induced activation of pro-inflammatory signaling, insulin resistance, and defective thermogenic-related responses upon cold exposure. Importantly, the decline in triiodothyronine (T3) levels in the circulation and intra-BAT after exposure of the WT mice to LPS and cold was markedly attenuated in the SIRT1Tg+ mice. In vitro BA experiments in the two genotypes revealed that upon differentiation with a T3-enriched medium and subsequent exposure to a macrophage-derived pro-inflammatory CM, only BA-SIRT1Tg+ fully recovered insulin and noradrenergic responses., [Conclusions]: This study has ascertained the benefit of the moderate overexpression of SIRT1 to confer protection against defective insulin and ß-adrenergic responses caused by BAT inflammation. Our results have potential therapeutic value in combinatorial therapies for BAT-specific thyromimetics and SIRT1 activators to combat metainflammation in this tissue., This study was funded by grants RTI2018-094052-B-100 (MICINN/AEI/FEDER, EU), S2017/BMD-3684 (Community of Madrid, Spain), the Ramón Areces Foundation (Spain), and CIBERdem (ISCIII) to A.M.V., grant S2010/BMD-2423 (Community of Madrid, Spain) to M.J.O. and A.M.V., grants SAF2017-83813-C3-1-R (MICINN/AEI/FEDER, EU) and CIBERobn (grant CB06/03/0001, ISCIII, Spain) to L.H. and D.S., grants 2017SGR278 (Government of Catalonia) and 201627-30 (Fundació La Marató of TV3) to D.S., grant RTI2018-095166-B-I00 (MICINN/AEI/FEDER, EU) to F.J.R., and grants SAF2017-86342-R (MICINN/AEI/FEDER, EU) and CIBERer (ISCIII, Spain) to A.G.-F. C.E. is a recipient of an FPU fellowship (Ministry of Universities, Spain) (FPU 15/00251). S.Z. is a recipient of an ANID fellowship from Chile.
Published: 2020

11. Covid-19: la malinterpretación de los datos de la pandemia daña la confianza del público

Author: Saez, Marc, Figueiras Guzmán, Adolfo, Alústiza Galarza, Ainhoa, Ruiz Cantero, Alberto, Medeiros de Figueiredo, Alexandre, Sánchez-Plà, Álex, Forte, Anabel, Cabrera-León, Andrés, Daponte Codina, Antonio, Tobías, Aurelio, Sánchez-Cantalejo Garrido, Carmen, Tebé, Cristian, Fernández Martínez, Daniel, Blanco de Tena-Dávila, David, Royé, Dominic, Cobo Valeri, Erik, Molist, Gemma, Gómez Melis, Guadalupe, Mateo Rodríguez, Inmaculada, Casillas, Jorge, Mateu, Jorge, Martínez, José A., González Alastrué, José Antonio, Caylà, Joan A., Guàrdia Olmos, juan, Barceló, Maria Antònia, Rueda García, María del Mar, Casals, Martí, Fuente Sánchez, Miguel de la, Fonseca i Casas, Pau, Macip, Salvador, Trias-Llimós, Sergi, Riffe, Tim, Cortés, Ulises, Consejo Superior de Investigaciones Científicas (España), Universidad de Valencia, Universidad de Barcelona, Universitat Oberta de Catalunya, Universidad de Granada, Universidad de Vic, Universitat Politècnica de Catalunya, Universidad Internacional de Cataluña, and Universidad de Santiago de Compostela
Subjects: Pandemia, COVID-19
Abstract: Dos cartas publicadas en The Lancet y The Lancet Public Health en los últimos meses defienden la necesidad de evaluar de forma independiente la respuesta española ante la covid-19. Estamos de acuerdo, pero nos gustaría complementarlas con tres puntos que nos acercan a la ciencia abierta. Nos referimos a la confusión terminológica, la calidad de los datos y su disponibilidad. Según las cartas, una de las razones por las que España se ha visto más afectada por la pandemia es la poca confianza que hay en el asesoramiento científico. Creemos que gran parte de esta desconfianza está ocasionada por una importante confusión terminológica. Esta se produce en prácticamente todos los medios de comunicación (con notables excepciones), y conduce a una malinterpretación de los datos y, como consecuencia, a una pérdida de confianza en los sistemas de información sanitaria, en la investigación y la epidemiología., Consejo Superior de Investigaciones Científicas aporta financiación como institución fundacional de The Conversation ES. Universitat de València, Universitat de Barcelona, UOC - Universitat Oberta de Catalunya, Universidad de Granada, Universitat de Vic - Universitat Central de Catalunya , Universitat Politècnica de Catalunya · BarcelonaTech, Universitat Internacional de Catalunya y Universidade de Santiago de Compostela aportan financiación como miembros de The Conversation ES.
Published: 2020

12. Neutralization of HSF1 in cells from PIK3CA-related overgrowth spectrum patients blocks abnormal proliferation

Author: Da Costa, Romain, De Almeida, Steven, Chevarin, Martin, Hadj-Rabia, Smail, Leclerc-Mercier, Stéphanie, Thauvin-Robinet, Christel, Garrido, Carmen, Faivre, Laurence, Vabres, Pierre, Duplomb, Laurence, Jego, Gaëtan, Equipe GAD (LNC - U1231), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, FHU TRANSLAD (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Equipe HSPpathies (LNC - U1231), and Service de Dermatologie (CHU de Dijon)
Subjects: [SDV]Life Sciences [q-bio]
Published: 2020

13. Protective role of SIRT1 activation against insulin resistance and defective thermogenesis associated to inflammation in brown adipose tissue

Author: Escalona Garrido, Carmen, Martínez Valverde, Angela, Vázquez Pérez, Patricia, UAM. Departamento de Bioquímica, and Instituto de Investigaciones Biomédicas 'Alberto Sols' (IIBM)
Subjects: Obesidad - Metabolismo - Tesis doctorales, Medicina, Diabetes tipo 2 - Tratamiento - Tesis doctorales
Abstract: Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Medicina, Departamento de Bioquímica. Fecha de lectura: 28-05-2020, Esta tesis tiene embargado el acceso al texto completo hasta el 28-11-2021
Published: 2020

14. Monitoring HSP70 exosomes in cancer patients' follow up: a clinical prospective pilot study

Author: Chanteloup, Gaëtan, Cordonnier, Marine, Isambert, Nicolas, Bertaut, Aurélie, Hervieu, Alice, Hennequin, Audrey, Luu, Maxime, Zanetta, Sylvie, Coudert, Bruno, Bengrine, Leila, Desmoulins, Isabelle, Favier, Laure, Lagrange, Aurélie, Pages, Pierre-Benoit, Gutierrez, Ivan, Lherminier, Jeanine, Avoscan, Laure, Jankowski, Clémentine, Rébé, Cédric, Chevriaux, Angélique, Padeano, Marie-Martine, Coutant, Charles, Ladoire, Sylvain, Causeret, Sylvain, Arnould, Laurent, Charon-Barra, Céline, Cottet, Vanessa, Blanc, Julie, Binquet, Christine, Bardou, Marc, Garrido, Carmen, Gobbo, Jessica, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer (LabEx LipSTIC), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-Université de Montpellier (UM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UFR des Sciences de Santé (Université de Bourgogne), Université de Bourgogne (UB), Laboratoire de neurosciences expérimentales et cliniques (LNEC), Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, CHU Dijon, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre d'Investigation Clinique 1432 (Dijon) - Epidemiologie Clinique/Essais Cliniques (CIC-EC), Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de chirurgie cardio-vasculaire et thoracique (CHU Dijon), Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and French Government grant ANR-11-LABX-0021-01-LipSTICLigue nationale contre le cancer Institut National du Cancer (INCA) France Centre Georges-Francois Leclerc Canceropole Est Fondation Silab Jean Paufique Regional Council Burgundy/Franche-Comte et FEDER Fondation pour la Recherche Medicale [FRM grant] ECO2016073609
Subjects: liquid biopsy, lcsh:Cytology, diagnosis, Short Communication, cancer, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, lcsh:QH573-671, Exosomes, HSP70
Abstract: International audience; Exosomes are nanovesicles released by all cells that can be found in the blood. A key point for their use as potential biomarkers in cancer is to differentiate tumour-derived exosomes from other circulating nanovesicles. Heat shock protein-70 (HSP70) has been shown to be abundantly expressed by cancer cells and to be associated with bad prognosis. We previously showed that exosomes derived from cancer cells carried HSP70 in the membrane while those from non-cancerous cells did not. In this work, we opened a prospective clinical pilot study including breast and lung cancer patients to determine whether it was possible to detect and quantify HSP70 exosomes in the blood of patients with solid cancers. We found that circulating exosomal HSP70 levels, but not soluble HSP70, reflected HSP70 content within the tumour biopsies. Circulating HSP70 exosomes increased in metastatic patients compared to non-metastatic patients or healthy volunteers. Further, we demonstrated that HSP70-exosome levels correlated with the disease status and, when compared with circulating tumour cells, were more sensitive tumour dissemination predictors. Finally, our case studies indicated that HSP70-exosome levels inversely correlated with response to the therapy and that, therefore, monitoring changes in circulating exosomal HSP70 might be useful to predict tumour response and clinical outcome.
Published: 2020

15. Monitoring HSP70 exosomes in cancer patients’ follow up: a clinical prospective pilot study

Author: Chanteloup, Gaëtan, Cordonnier, Marine, Isambert, Nicolas, Bertaut, Aurélie, Hervieu, Alice, Hennequin, Audrey, Luu, Maxime, Zanetta, Sylvie, Coudert, Bruno, Bengrine, Leila, Desmoulins, Isabelle, Favier, Laure, Lagrange, Aurélie, Pages, Pierre-Benoit, Gutierrez, Ivan, Lherminier, Jeanine, Avoscan, Laure, Jankowski, Clémentine, Rébé, Cédric, Chevriaux, Angélique, Padeano, Marie-Martine, Coutant, Charles, Ladoire, Sylvain, Causeret, Sylvain, Arnould, Laurent, Charon-Barra, Céline, Cottet, Vanessa, Blanc, Julie, Binquet, Christine, Bardou, Marc, Garrido, Carmen, Gobbo, Jessica, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer (LabEx LipSTIC), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-Université de Montpellier (UM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UFR des Sciences de Santé (Université de Bourgogne), Université de Bourgogne (UB), Laboratoire de neurosciences expérimentales et cliniques (LNEC), Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, CHU Dijon, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre d'Investigation Clinique 1432 (Dijon) - Epidemiologie Clinique/Essais Cliniques (CIC-EC), Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de chirurgie cardio-vasculaire et thoracique (CHU Dijon), Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French Government grant ANR-11-LABX-0021-01-LipSTICLigue nationale contre le cancer Institut National du Cancer (INCA) France Centre Georges-Francois Leclerc Canceropole Est Fondation Silab Jean Paufique Regional Council Burgundy/Franche-Comte et FEDER Fondation pour la Recherche Medicale [FRM grant] ECO2016073609, and Lallemant, Christopher
Subjects: liquid biopsy, diagnosis, cancer, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Exosomes, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, HSP70
Abstract: International audience; Exosomes are nanovesicles released by all cells that can be found in the blood. A key point for their use as potential biomarkers in cancer is to differentiate tumour-derived exosomes from other circulating nanovesicles. Heat shock protein-70 (HSP70) has been shown to be abundantly expressed by cancer cells and to be associated with bad prognosis. We previously showed that exosomes derived from cancer cells carried HSP70 in the membrane while those from non-cancerous cells did not. In this work, we opened a prospective clinical pilot study including breast and lung cancer patients to determine whether it was possible to detect and quantify HSP70 exosomes in the blood of patients with solid cancers. We found that circulating exosomal HSP70 levels, but not soluble HSP70, reflected HSP70 content within the tumour biopsies. Circulating HSP70 exosomes increased in metastatic patients compared to non-metastatic patients or healthy volunteers. Further, we demonstrated that HSP70-exosome levels correlated with the disease status and, when compared with circulating tumour cells, were more sensitive tumour dissemination predictors. Finally, our case studies indicated that HSP70-exosome levels inversely correlated with response to the therapy and that, therefore, monitoring changes in circulating exosomal HSP70 might be useful to predict tumour response and clinical outcome.
Published: 2020

16. Contexto macroeconómico y polı́tico nacional que fomentó el comercio electrónico en los primeros años del 2000 en la Argentina

Author: Garrido, Carmen Alicia and Kosacoff, Bernardo P.
Subjects: Comercio electrónico, Inversión extranjera, Privatización de empresas, Análisis FODA
Abstract: Fil: Garrido, Carmen Alicia. Universidad de Buenos Aires. Facultad de Ciencias Económicas
Published: 2020

17. La publicidad en tiempos de guerra (1936-1939): un estudio de la publicidad en el ABC republicano

Author: Romero Garrido, Carmen, Langa-Nuño, Concha, and Universidad de Sevilla. Departamento de Historia Contemporánea
Subjects: Guerra Civil Española, Publicidad, República Española, Historia de España, ABC
Abstract: Este Trabajo de Fin de Grado tiene como objetivo el estudio de la publicidad insertada en un soporte específico, la prensa, más concretamente en el periódico ABC, y más concretamente en un periodo tan excepcional como lo fue la guerra civil española. La elección viene de la existencia de dos ediciones opuestas en estas circunstancias, como veremos explicado más adelante. Por ello nos centraremos en el ABC de Madrid, periódico republicano, durante los tres primeros meses de la guerra (julio, agosto y septiembre de 1936). Antes, procederemos al repaso de la historia de la Guerra Civil Española para contextualizar las razones de todo lo sucedido en prensa y publicidad en este periodo de la Historia de España. Universidad de Sevilla. Grado en Publicidad y Relaciones Públicas
Published: 2020

18. Chaperoning STAT3/5 by Heat Shock Proteins: Interest of Their Targeting in Cancer Therapy

Author: Jego, Gaëtan, Hermetet, François, Girodon, François, and Garrido, Carmen
Subjects: heat shock proteins, chaperones, Review, targeted therapy, stabilization
Abstract: While cells from multicellular organisms are dependent upon exogenous signals for their survival, growth, and proliferation, commitment to a specific cell fate requires the correct folding and maturation of proteins, as well as the degradation of misfolded or aggregated proteins within the cell. This general control of protein quality involves the expression and the activity of molecular chaperones such as heat shock proteins (HSPs). HSPs, through their interaction with the STAT3/STAT5 transcription factor pathway, can be crucial both for the tumorigenic properties of cancer cells (cell proliferation, survival) and for the microenvironmental immune cell compartment (differentiation, activation, cytokine secretion) that contributes to immunosuppression, which, in turn, potentially promotes tumor progression. Understanding the contribution of chaperones such as HSP27, HSP70, HSP90, and HSP110 to the STAT3/5 signaling pathway has raised the possibility of targeting such HSPs to specifically restrain STAT3/5 oncogenic functions. In this review, we present how HSPs control STAT3 and STAT5 activation, and vice versa, how the STAT signaling pathways modulate HSP expression. We also discuss whether targeting HSPs is a valid therapeutic option and which HSP would be the best candidate for such a strategy.
Published: 2019

19. XPO1 regulates erythroid differentiation and is a new target for the treatment of β-thalassemia

Author: Guillem, Flavia, Dussiot, Michael, Colin, Elia, Suriyun, Thunwarat, Arlet, Jean Benoit, Goudin, Nicolas, Marcion, Guillaume, Seigneuric, Renaud, Causse, Sebastien, Gonin, Patrick, Gastou, Marc, Deloger, Marc, Rossignol, Julien, Lamarque, Mathilde, Bellaid Choucair, Zakia, Gautier, Emilie Fleur, Ducamp, Sarah, Vandekerckhove, Julie, Moura, Ivan, Maciel, Thiago, Garrido, Carmen, An, Xiuli, Mayeux, Patrick, Mohandas, Narla, COURTOIS, genevieve, Hermine, Olivier, Centre de recherche biomédicale Bichat-Beaujon (CRB3), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plateforme d'Imagerie Cellulaire [SFR Necker], Structure Fédérative de Recherche Necker (SFR Necker - UMS 3633 / US24), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer (LabEx LipSTIC), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Paris-Sud - Paris 11 (UP11)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-Université de Montpellier (UM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Institut Gustave Roussy (IGR), Hématopoïèse normale et pathologique (U1170 Inserm), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Eléments transposables, évolution, populations, Département génétique, interactions et évolution des génomes [LBBE] (GINSENG), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Cytokines, hématopoïèse et réponse immune (CHRI), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service d'immuno-hématologie pédiatrique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Angiogénèse embryonnaire et pathologique, Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Labex MemoLife, Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Red Cell Physiology Laboratory [New York, USA], New York Blood Center, Service d'hématolgie adulte, Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut des Maladies Génétiques Imagine [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Paris-Sud - Paris 11 (UP11)-École pratique des hautes études (EPHE)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc (CRLCC - CGFL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-France-Comté] (EFS [Bourgogne-France-Comté])-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Université de Franche-Comté (UFC)-Université de Montpellier (UM), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Université Pierre et Marie Curie - Paris 6 (UPMC)-Collège de France (CdF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Paris-Sud - Paris 11 (UP11)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Montpellier (UM), Centre interdisciplinaire de recherche en biologie (CIRB), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), COURTOIS, genevieve, and UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-Université de Montpellier (UM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)
Subjects: [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, hemic and lymphatic diseases, Article, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: β-thalassemia major (β-TM) is an inherited hemoglobinopathy caused by a quantitative defect in the synthesis of β-globin chains of hemoglobin, leading to the accumulation of free a-globin chains that aggregate and cause ineffective erythropoiesis. We have previously demonstrated that terminal erythroid maturation requires a transient activation of caspase-3 and that the chaperone Heat Shock Protein 70 (HSP70) accumulates in the nucleus to protect GATA-1 transcription factor from caspase-3 cleavage. This nuclear accumulation of HSP70 is inhibited in human β-TM erythroblasts due to HSP70 sequestration in the cytoplasm by free a-globin chains, resulting in maturation arrest and apoptosis. Likewise, terminal maturation can be restored by transduction of a nuclear-targeted HSP70 mutant. Here we demonstrate that in normal erythroid progenitors, HSP70 localization is regulated by the exportin-1 (XPO1), and that treatment of β-thalassemic erythroblasts with an XPO1 inhibitor increased the amount of nuclear HSP70, rescued GATA-1 expression and improved terminal differentiation, thus representing a new therapeutic option to ameliorate ineffective erythropoiesis of β-TM.
Published: 2019

20. Beta 3 adrenoceptor signalling pathway inhibites inflammasome activity in macrophages

Author: Zhang, H., Lopez, T., Neiers, Fabrice, Bouysse, E., Wendremaire, Maeva, Garrido, Carmen, Lirussi, Frédéric, UMR 1231, Institut National de la Santé et de la Recherche Médicale (INSERM), Université Bourgogne Franche-Comté [COMUE] (UBFC), Zhejiang University, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Dijon (CHU de Dijon), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)
Subjects: [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], education, [SDV.IMM]Life Sciences [q-bio]/Immunology, health care economics and organizations
Abstract: Meeting Abstract P1246 in Abstracts of IUIS 2019 Beijing.; Beta 3 adrenoceptor signalling pathway inhibites inflammasome activity in macrophages. 17. International Congress of Immunology (IUIS 2019)
Published: 2019

21. Exosomal miRNA: Small Molecules, Big Impact in Colorectal Cancer

Author: Vautrot, Valentin, Chanteloup, Gaëtan, Elmallah, Mohammed, Cordonnier, Marine, Aubin, François, Garrido, Carmen, and Gobbo, Jessica
Subjects: Article Subject
Abstract: Colorectal cancer (CRC) is one of the major causes of cancer-related deaths worldwide. Tumor microenvironment (TME) contains many cell types including stromal cells, immune cells, and endothelial cells. The TME modulation explains the heterogeneity of response to therapy observed in patients. In this context, exosomes are emerging as major contributors in cancer biology. Indeed, exosomes are implicated in tumor proliferation, angiogenesis, invasion, and premetastatic niche formation. They contain bioactive molecules such as proteins, lipids, and RNAs. More recently, many studies on exosomes have focused on miRNAs, small noncoding RNA molecules able to influence protein expression. In this review, we describe miRNAs transported by exosomes in the context of CRC and discuss their influence on TME and their potential as circulating biomarkers. This overview underlines emerging roles for exosomal miRNAs in cancer research for the near future.
Published: 2019
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22. ADRB3 stimulation induced M2-like phenotype in in vitro primary human macrophages

Author: Lopez, Tatiana, Zhang, H., Neiers, Fabrice, Bouysse, E., Wendremaire, Maeva, Garrido, Carmen, Lirussi, Frederic, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Zhejiang University, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and ProdInra, Migration
Subjects: [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.IMM] Life Sciences [q-bio]/Immunology, [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], Immunologie, Immunology, [SDV.IMM]Life Sciences [q-bio]/Immunology, ComputingMilieux_MISCELLANEOUS, Autre (Sciences du Vivant)
Abstract: Meeting Abstract P1929 in Abstracts of IUIS 2019 Beijing.; International audience
Published: 2019

23. Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018

Author: Galluzzi, Lorenzo, Vitale, Ilio, Aaronson, Stuart A, Abrams, John M, Adam, Dieter, Agostinis, Patrizia, Alnemri, Emad S, Altucci, Lucia, Amelio, Ivano, Andrews, David W, Annicchiarico-Petruzzelli, Margherita, Antonov, Alexey V, Arama, Eli, Baehrecke, Eric H, Barlev, Nickolai A, Bazan, Nicolas G, Bernassola, Francesca, Bertrand, Mathieu JM, Bianchi, Katiuscia, Blagosklonny, Mikhail V, Blomgren, Klas, Borner, Christoph, Boya, Patricia, Brenner, Catherine, Campanella, Michelangelo, Candi, Eleonora, Carmona-Gutierrez, Didac, Cecconi, Francesco, Chan, Francis K-M, Chandel, Navdeep S, Cheng, Emily H, Chipuk, Jerry E, Cidlowski, John A, Ciechanover, Aaron, Cohen, Gerald M, Conrad, Marcus, Cubillos-Ruiz, Juan R, Czabotar, Peter E, D'Angiolella, Vincenzo, Dawson, Ted M, Dawson, Valina L, De Laurenzi, Vincenzo, De Maria, Ruggero, Debatin, Klaus-Michael, DeBerardinis, Ralph J, Deshmukh, Mohanish, Di Daniele, Nicola, Di Virgilio, Francesco, Dixit, Vishva M, Dixon, Scott J, Duckett, Colin S, Dynlacht, Brian D, El-Deiry, Wafik S, Elrod, John W, Fimia, Gian Maria, Fulda, Simone, García-Sáez, Ana J, Garg, Abhishek D, Garrido, Carmen, Gavathiotis, Evripidis, Golstein, Pierre, Gottlieb, Eyal, Green, Douglas R, Greene, Lloyd A, Gronemeyer, Hinrich, Gross, Atan, Hajnoczky, Gyorgy, Hardwick, J Marie, Harris, Isaac S, Hengartner, Michael O, Hetz, Claudio, Ichijo, Hidenori, Jäättelä, Marja, Joseph, Bertrand, Jost, Philipp J, Juin, Philippe P, Kaiser, William J, Karin, Michael, Kaufmann, Thomas, Kepp, Oliver, Kimchi, Adi, Kitsis, Richard N, Klionsky, Daniel J, Knight, Richard A, Kumar, Sharad, Lee, Sam W, Lemasters, John J, Levine, Beth, Linkermann, Andreas, Lipton, Stuart A, Lockshin, Richard A, López-Otín, Carlos, Lowe, Scott W, Luedde, Tom, Lugli, Enrico, MacFarlane, Marion, Madeo, Frank, Malewicz, Michal, Malorni, Walter, and Manic, Gwenola
Subjects: Necrosis, Biochemistry & Molecular Biology, Cell Death, Mitochondrial Permeability Transition Pore, Animals, Humans, Biological Sciences, Lysosomes, Mitochondrial Membrane Transport Proteins, Medical and Health Sciences
Abstract: Over the past decade, the Nomenclature Committee on Cell Death (NCCD) has formulated guidelines for the definition and interpretation of cell death from morphological, biochemical, and functional perspectives. Since the field continues to expand and novel mechanisms that orchestrate multiple cell death pathways are unveiled, we propose an updated classification of cell death subroutines focusing on mechanistic and essential (as opposed to correlative and dispensable) aspects of the process. As we provide molecularly oriented definitions of terms including intrinsic apoptosis, extrinsic apoptosis, mitochondrial permeability transition (MPT)-driven necrosis, necroptosis, ferroptosis, pyroptosis, parthanatos, entotic cell death, NETotic cell death, lysosome-dependent cell death, autophagy-dependent cell death, immunogenic cell death, cellular senescence, and mitotic catastrophe, we discuss the utility of neologisms that refer to highly specialized instances of these processes. The mission of the NCCD is to provide a widely accepted nomenclature on cell death in support of the continued development of the field.
Published: 2018

24. Heat shock protein-90 toward theranostics: a breath of fresh air in idiopathic pulmonary fibrosis

Author: Bonniaud , Philippe, Burgy , Olivier, Garrido , Carmen, Service de Pneumologie Soins Intensifs, Appareillage Respiratoire [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Université Bourgogne Franche-Comté ( UBFC )
Subjects: [ SDV.MHEP.PSR ] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Abstract: IF 10.569; International audience
Published: 2018

25. Heat shock protein-90 toward theranostics: a breath of fresh air in idiopathic pulmonary fibrosis

Author: Bonniaud , Philippe, Burgy , Olivier, Garrido , Carmen, Service de Pneumologie Soins Intensifs, Appareillage Respiratoire [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Université Bourgogne Franche-Comté ( UBFC )
Subjects: [ SDV.MHEP.PSR ] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Abstract: IF 10.569; International audience
Published: 2018

26. Heat shock protein-90 toward theranostics: a breath of fresh air in idiopathic pulmonary fibrosis

Author: Bonniaud , Philippe, Burgy , Olivier, Garrido , Carmen, Service de Pneumologie Soins Intensifs, Appareillage Respiratoire [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Université Bourgogne Franche-Comté ( UBFC )
Subjects: [ SDV.MHEP.PSR ] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Abstract: IF 10.569; International audience
Published: 2018

27. Heat shock protein-90 toward theranostics: a breath of fresh air in idiopathic pulmonary fibrosis

Author: Bonniaud , Philippe, Burgy , Olivier, Garrido , Carmen, Service de Pneumologie Soins Intensifs, Appareillage Respiratoire [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Université Bourgogne Franche-Comté ( UBFC )
Subjects: [ SDV.MHEP.PSR ] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Abstract: IF 10.569; International audience
Published: 2018

28. Influences and confluences of classical psychology in social work

Author: Puente Ferreras, Aníbal, Garrido, Carmen Gloria, and Marín, Mª Isabel
Subjects: Trabajo Social, social work, Conductismo, lcsh:HN1-995, social evolutionism, Evolucionismo Social, Psicología, Escuelas Psicológicas, Gestalt, behaviourism, lcsh:H1-99, schools of psychology, lcsh:Social history and conditions. Social problems. Social reform, lcsh:Social sciences (General)
Abstract: This article analyses the influences of psychology on social work. An examination of classical approaches reveals that some have a more significant influence than others: a) evolutionary psychology has a highly negative effect on fairness among persons; b) psychoanalysis impacts on the family as a social entity, c) behaviourism designs models for changing social behaviour; and d) humanism, existentialism and Gestalt psychology use empathy as a means to improve communication. These theoretical models and psychological methods are socially efficient in influencing the professional tasks and practices of social workers in various fields. El artículo analiza las influencias psicológicas en el Trabajos Social. Al examinar los enfoques clásicos se observa que algunos influyen de una manera más significativa que otros: a) el evolucionismo produce un efecto muy negativo sobre la equidad entre las personas; b) el psicoanálisis impronta en la familia como entidad social; c) el conductismo diseña modelos de cambio de conducta social; d) el humanismo, existencialismo y Gestalt usan la empatía como medio para mejorar la comunicación. Estos modelos teóricos y métodos psicológicos intervienen con eficiencia social en las tareas y desempeños profesionales de los trabajadores sociales en diversos ámbitos.
Published: 2018

29. The vesicular transfer of CLIC1 from glioblastoma to microvascular endothelial cells requires TRPM7

Author: Thuringer, Dominique, Chanteloup, Gaetan, Winckler, Pascale, Garrido, Carmen, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), and UNICANCER
Subjects: transient receptor potential melastatin, microRNA, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, glioblastoma, exosome, [SDV.CAN]Life Sciences [q-bio]/Cancer, chloride intracellular channel
Abstract: International audience; Chloride intracellular channel 1 (CLIC1) is highly expressed and secreted by human glioblastoma cells and cell lines such as U87, initiating cell migration and tumor growth. Here, we examined whether CLIC1 could be transferred to human primary microvascular endothelial cells (HMEC). We previously reported that the oncogenic microRNA, miR-5096, increased the release of extracellular vesicles (EVs) by which it increased its own transfer from U87 to surrounding cells. Thus, we also examined its effect on the CLIC1 transfer. In homotypic cultures, miR-5096 did not increase the expression of CLIC1 in U87 nor in HMEC. However, the endothelial CLIC1 level increased after exposure to EVs released by U87, and even more by miR-5096-loaded U87. The EVs-transferred CLIC1 was active in HMEC, promoting endothelial sprouting in matrigel. Cell exposure to EVs induced cytosolic Ca 2+ spikes which were dependent on the transient receptor potential melastatin member 7 (TRPM7). TRPM7 silencing prevented Ca 2+ spikes and the subsequent CLIC1 delivery into HMEC. Our data suggest that the vesicular transfer of CLIC1 between cells requires TRMP7 expression in recipient endothelial cells. How the vesicular transfer of CLIC1 is modulated in cancer therapy is a future challenge.
Published: 2018

30. E2F1 binds to the peptide-binding groove within the BIR3 domain of cIAP1 and requires cIAP1 for chromatin binding

Author: Allègre, Jennifer, Cartier, Jessy, Glorian, Valérie, Nathalie, Droin, Dumetier, Baptiste, Kayaci, Cémile, Berthelet, Jean, Gemble, Simon, Vuillier, Céline, Maillet, Laurent, Garrido, Carmen, Dubrez, Laurence, université de Bourgogne, LNC, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université Bourgogne Franche-Comté [COMUE] (UBFC), Hématopoïèse normale et pathologique (U1170 Inserm), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Stress Adaptation and Tumor Escape in Breast Cancer (CRCINA-ÉQUIPE 8), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)
Subjects: endocrine system, Cultured tumor cells, Synthesis Phase, Gene Expression, lcsh:Medicine, Cell Communication, Biochemistry, Cell Line, Inhibitor of Apoptosis Proteins, Protein Domains, Gene Types, DNA-binding proteins, Genetics, Humans, Immunoprecipitation, Gene Regulation, HeLa cells, Cell Cycle and Cell Division, Post-Translational Modification, lcsh:Science, Binding Sites, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Chromosome Biology, lcsh:R, Ubiquitination, Biology and Life Sciences, Proteins, Cell Biology, Cell cultures, Chromatin, Regulatory Proteins, Precipitation Techniques, Research and analysis methods, Cell Processes, Cell lines, Regulator Genes, Epigenetics, lcsh:Q, biological phenomena, cell phenomena, and immunity, Biological cultures, E2F1 Transcription Factor, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Protein Binding, Signal Transduction, Research Article, Transcription Factors
Abstract: International audience; The cellular inhibitor of apoptosis 1 (cIAP1) is an E3-ubiquitin ligase that regulates cell signaling pathways involved in fundamental cellular processes including cell death, cell proliferation, cell differentiation and inflammation. It recruits ubiquitination substrates thanks to the presence of three baculoviral IAP repeat (BIR) domains at its N-terminal extremity. We previously demonstrated that cIAP1 promoted the ubiquitination of the E2 factor 1 (E2F1) transcription factor. Moreover, we showed that cIAP1 was required for E2F1 stabilization during the S phase of cell cycle and in response to DNA damage. Here, we report that E2F1 binds within the cIAP1 BIR3 domain. The BIR3 contains a surface hydrophobic groove that specifically anchors a conserved IAP binding motif (IBM) found in a number of intracellular proteins including Smac. The Smac N-7 peptide that includes the IBM, as well as a Smac mimetic, competed with E2F1 for interaction with cIAP1 demonstrating the importance of the BIR surface hydrophobic groove. We demonstrated that the first alpha-helix of BIR3 was required for E2F1 binding, as well as for the binding of Smac and Smac mimetics. Overexpression of cIAP1 modified the ubiquitination profile of E2F1, increasing the ratio of E2F1 conjugated with K11- and K63-linked ubiquitin chains, and decreasing the proportion of E2F1 modified by K48-linked ubiquitin chains. ChIP-seq analysis demonstrated that cIAP1 was required for the recruitment of E2F1 onto chromatin. Lastly, we identified an E2F-binding site on the cIAP1-encoding birc2 gene promoter, suggesting a retro-control regulation loop.
Published: 2018

31. Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018

Author: Galluzzi, Lorenzo, Vitale, Ilio, Aaronson, Stuart A., Abrams, John M., Adam, Dieter, Agostinis, Patrizia, Alnemri, Emad S., Altucci, Lucia, Amelio, Ivano, Andrews, David W., Annicchiarico-Petruzzelli, Margherita, Antonov, Alexey V., Arama, Eli, Baehrecke, Eric H., Barlev, Nickolai A., Bazan, Nicolas G., Bernassola, Francesca, Bertrand, Mathieu J. M., Bianchi, Katiuscia, Blagosklonny, Mikhail V., Blomgren, Klas, Borner, Christoph, Boya, Patricia, Brenner, Catherine, Campanella, Michelangelo, Candi, Eleonora, Carmona-Gutierrez, Didac, Cecconi, Francesco, Chan, Francis K.-M., Chandel, Navdeep S., Cheng, Emily H., Chipuk, Jerry E., Cidlowski, John A., Ciechanover, Aaron, Cohen, Gerald M., Conrad, Marcus, Cubillos-Ruiz, Juan R., Czabotar, Peter E., D’Angiolella, Vincenzo, Dawson, Ted M., Dawson, Valina L., De Laurenzi, Vincenzo, De Maria, Ruggero, Debatin, Klaus-Michael, DeBerardinis, Ralph J., Deshmukh, Mohanish, Di Daniele, Nicola, Di Virgilio, Francesco, Dixit, Vishva M., Dixon, Scott J., Duckett, Colin S., Dynlacht, Brian D., El-Deiry, Wafik S., Elrod, John W., Fimia, Gian Maria, Fulda, Simone, García-Sáez, Ana J., Garg, Abhishek D., Garrido, Carmen, Gavathiotis, Evripidis, Golstein, Pierre, Gottlieb, Eyal, Green, Douglas R., Greene, Lloyd A., Gronemeyer, Hinrich, Gross, Atan, Hajnoczky, Gyorgy, Hardwick, J. Marie, Harris, Isaac S., Hengartner, Michael O., Hetz, Claudio, Ichijo, Hidenori, Jäättelä, Marja, Joseph, Bertrand, Jost, Philipp J., Juin, Philippe P., Kaiser, William J., Karin, Michael, Kaufmann, Thomas, Kepp, Oliver, Kimchi, Adi, Kitsis, Richard N., Klionsky, Daniel J., Knight, Richard A., Kumar, Sharad, Lee, Sam W., Lemasters, John J., Levine, Beth, Linkermann, Andreas, Lipton, Stuart A., Lockshin, Richard A., López-Otín, Carlos, Lowe, Scott W., Luedde, Tom, Lugli, Enrico, MacFarlane, Marion, Madeo, Frank, Malewicz, Michal, Malorni, Walter, Manic, Gwenola, Marine, Jean-Christophe, Martin, Seamus J., Martinou, Jean-Claude, Medema, Jan Paul, Mehlen, Patrick, Meier, Pascal, Melino, Sonia, Miao, Edward A., Molkentin, Jeffery D., Moll, Ute M., Muñoz-Pinedo, Cristina, Nagata, Shigekazu, Nuñez, Gabriel, Oberst, Andrew, Oren, Moshe, Overholtzer, Michael, Pagano, Michele, Panaretakis, Theocharis, Pasparakis, Manolis, Penninger, Josef M., Pereira, David M., Pervaiz, Shazib, Peter, Marcus E., Piacentini, Mauro, Pinton, Paolo, Prehn, Jochen H.M., Puthalakath, Hamsa, Rabinovich, Gabriel A., Rehm, Markus, Rizzuto, Rosario, Rodrigues, Cecilia M.P., Rubinsztein, David C., Rudel, Thomas, Ryan, Kevin M., Sayan, Emre, Scorrano, Luca, Shao, Feng, Shi, Yufang, Silke, John, Simon, Hans-Uwe, Sistigu, Antonella, Stockwell, Brent R., Strasser, Andreas, Szabadkai, Gyorgy, Tait, Stephen W.G., Tang, Daolin, Tavernarakis, Nektarios, Thorburn, Andrew, Tsujimoto, Yoshihide, Turk, Boris, Vanden Berghe, Tom, Vandenabeele, Peter, Vander Heiden, Matthew G., Villunger, Andreas, Virgin, Herbert W., Vousden, Karen H., Vucic, Domagoj, Wagner, Erwin F., Walczak, Henning, Wallach, David, Wang, Ying, Wells, James A., Wood, Will, Yuan, Junying, Zakeri, Zahra, Zhivotovsky, Boris, Zitvogel, Laurence, Melino, Gerry, and Kroemer, Guido
Subjects: ddc
Published: 2018

32. Increased FGF21 in brown adipose tissue of tyrosine hydroxylase heterozygous mice: implications for cold adaptation

Author: Vázquez Pérez, Patricia, Hernández-Sánchez, Catalina, Escalona-Garrido, Carmen, Pereira, Laura, Contreras, Cristina, López, Miguel, Balsinde, Jesús, Pablo, Flora de, Valverde, Ángela M., Ministerio de Ciencia e Innovación (España), Instituto de Salud Carlos III, European Commission, Hernández-Sánchez, Catalina [0000-0002-0846-5019], Contreras, Cristina [0000-0002-7015-7922], Balsinde, Jesús [0000-0002-4157-6714], Pablo, Flora de [0000-0002-8357-5074 ], and Valverde, Ángela M. [0000-0003-1192-9045]
Subjects: Catecholamines, FGF21, Diabetes, UCP-1, PRDM16, Adipose tissue, Metabolic disease, Obesity, Fatty acids, Brown adipose tissue
Abstract: Tyrosine hydroxylase (TH) catalyzes the first step in catecholamines synthesis. We studied the impact of reduced TH in brown adipose tissue (BAT) activation. In adult heterozygous (Th+/-) mice, dopamine and noradrenaline (NA) content in BAT decreased after cold exposure. This reduced catecholaminergic response did not impair cold adaptation, since these mice induced uncoupling protein-1 (UCP1) and maintained BAT temperature to a similar extent than controls (Th+/+). Possible compensatory mechanisms implicated were studied. Prdm16 and Fgf21 expression, key genes in BAT activation, were elevated in Th+/- mice at thermoneutrality from day 18.5 of embryonic life. Likewise, plasma FGF21 and liver Fgf21 mRNA were increased. Analysis of endoplasmic reticulum (ER) stress, a process that triggers elevations in FGF21, showed higher phospho-IRE1, phospho-JNK and CHOP in BAT of Th+/- mice at thermoneutrality. Also, increased lipolysis in BAT of coldexposure Th+/- mice was demonstrated by increased phosphorylation of hormone-sensitive lipase (HSL), as well as diacylglycerol (DAG) and free fatty acids (FFA) content. Overall, these results indicate that the mild effects of Th haploinsufficiency on BAT function are likely due to compensatory mechanisms involving elevations in Fgf21 and Prdm16 and through adaptive changes in the lipid profile. This work was supported by a Ministry of Science, Technology and Innovation grants BFU 2010-15868 to FdeP and CHS, SAF2015-73000-EXP and SAF2016-80883 to JB and SAF2015-65267-R to AMV. We also acknowledge grant INFLAMES (ISCIII PIE14/00045, co-funded by ERDF, “Investing in your future”) to AMV and Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas CIBERdem This is an initiative of the Instituto de Salud Carlos III (Madrid,Spain). We thank MICINN-CSIC and Fondo Social Europeo (FSE) for the JAE-DOC contract (2013 to PV) and CIBERdem contract (to PV 2016).
Published: 2018

33. Modulation of the inwardly rectifying potassium channel Kir4.1 by the pro-invasive miR-5096 in glioblastoma cells

Author: Thuringer, Dominique, Chanteloup, Gaetan, Boucher, Jonathan, Pernet, Nicolas, Boudesco, Christophe, Jego, Gaetan, Chatelier, Aurelien, Bois, Patrick, Gobbo, Jessica, Cronier, Laurent, Solary, Eric, Garrido, Carmen, Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Signalisation et Transports Ioniques Membranaires ( STIM ), Université de Poitiers-Centre National de la Recherche Scientifique ( CNRS ), Université Bourgogne Franche-Comté ( UBFC ), Département d'oncologie médicale [Centre Georges-François Leclerc], Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ), Institut Gustave Roussy ( IGR ), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER, Institut Gustave Roussy (IGR), and Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: microRNA, [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Transfection, migration, K+ current, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, MicroRNAs, filopodia, Cell Movement, Humans, exosome, Potassium Channels, Inwardly Rectifying, Glioblastoma, Cells, Cultured, Pentamidine, Research Paper
Abstract: IF 5.168; International audience; Inwardly rectifying potassium channels (Kir), and especially the barium-sensitive Kir4.1 encoded by KCNJ10, are key regulators of glial functions. A lower expression or mislocation of Kir4.1 is detected in human brain tumors. MicroRNAs participate in the regulation of ionic channels and associated neurologic disorders. Here, we analyze effects of miR-5096 on the Kir4.1 expression and function in two glioblastoma cell lines, U87 and U251. Using whole-cell patch-clamp and western-blot analysis, we show that cell loading with miR-5096 decreases the Kir4.1 protein level and associated K+ current. Cell treatment with barium, a Kir4.1 blocker, or cell loading of miR-5096 both increase the outgrowth of filopodia in glioma cells, as observed by time-lapse microscopy. Knocking-down Kir4.1 expression by siRNA transfection similarly increased both filopodia formation and invasiveness of glioma cells as observed in Boyden chamber assay. MiR-5096 also promotes the release of extracellular vesicles by which it increases its own transfer to surrounding cells, in a Kir4.1-dependent manner in U251 but not in U87. Altogether, our results validate Kir4.1 as a miR-5096 target to promote invasion of glioblastoma cells. Our data highlight the complexity of microRNA effects and the role of K+ channels in cancer.
Published: 2017

34. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues

Author: Glorian, Valérie, Allègre, Jennifer, Berthelet, Jean, Dumetier, Baptiste, Boutanquoi, Pierre-Marie, Droin, Nathalie, Kayaci, Cémile, Cartier, Jessy, Gemble, Simon, Marcion, Guillaume, Gonzalez, Daniel, Boidot, Romain, Garrido, Carmen, Michaud, Olivier, Solary, Eric, Dubrez, Laurence, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Hématopoïèse normale et pathologique (U1170 Inserm), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, Faculté de Médecine Paris Sud, Le Kremlin-Bicêtre, Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Hématopoïèse normale et pathologique ( U1170 Inserm ), Université Paris-Sud - Paris 11 ( UP11 ) -Institut Gustave Roussy ( IGR ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: endocrine system, Transcription, Genetic, polysialic acid, HDL, Ubiquitin-Protein Ligases, Arginine, Methylation, Inhibitor of Apoptosis Proteins, S Phase, LDL, Mice, Structure-Activity Relationship, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, Animals, Humans, hypothalamus, Polyubiquitin, synaptic plasticity, Protein Stability, Lysine, Ubiquitination, Correction, Original Article, biological phenomena, cell phenomena, and immunity, atherosclerosis, E2F1 Transcription Factor, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, DNA Damage
Abstract: IF 5.965; International audience; The E2F transcription factor 1 is subtly regulated along the cell cycle progression and in response to DNA damage by post-translational modifications. Here, we demonstrated that the E3-ubiquitin ligase cellular inhibitor of apoptosis 1 (cIAP1) increases E2F1 K63-poly-ubiquitination on the lysine residue 161/164 cluster, which is associated with the transcriptional factor stability and activity. Mutation of these lysine residues completely abrogates the binding of E2F1 to CCNE, TP73 and APAF1 promoters, thus inhibiting transcriptional activation of these genes and E2F1-mediated cell proliferation control. Importantly, E2F1 stabilization in response to etoposide-induced DNA damage or during the S phase of cell cycle, as revealed by cyclin A silencing, is associated with K63-poly-ubiquitinylation of E2F1 on lysine 161/164 residues and involves cIAP1. Our results reveal an additional level of regulation of the stability and the activity of E2F1 by a non-degradative K63-poly-ubiquitination and uncover a novel function for the E3-ubiquitin ligase cIAP1.
Published: 2017

35. N-glycosylation of mouse TRAIL-R and human TRAIL-R1 enhances TRAIL-induced death : N-glycosylation of TRAIL receptors

Author: Dufour, Florent, Rattier, Thibault, Shirley, Sarah, Picarda, Gaelle, Constantinescu, Andrei Alexandru, Morlé, Aymeric, Zakaria, Al Batoul, Marcion, Guillaume, Causse, Sebastien, Szegezdi, eva, Zajonc, Dirk Michael, Seigneuric, Renaud, Guichard, Gilles, Gharbi, Tijani, Picaud, Fabien, Herlem, Guillaume, Garrido, Carmen, Schneider, Pascal, Benedict, Chris Alan, Micheau, Olivier, Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Equipe HSPpathies (LNC - U1231), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), La Jolla Institute for Allergy & Immunology ( LA JOLLA Institute ), Nanomédecine, imagerie, thérapeutique - UFC ( NIT / NANOMEDECINE ), Université de Franche-Comté ( UFC ) -Université Bourgogne Franche-Comté ( UBFC ), Apoptosis Research Centre [Galway, Ireland] ( ARC ), National University of Ireland [Galway] ( NUI Galway ), Ghent University [Belgium] ( UGENT ), Chimie et Biologie des Membranes et des Nanoobjets ( CBMN ), Université Sciences et Technologies - Bordeaux 1-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux ( ENITAB ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer ( LabEx LipSTIC ), Institut National de la Recherche Agronomique ( INRA ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Université Paris-Sud - Paris 11 ( UP11 ) -École pratique des hautes études ( EPHE ) -Institut Gustave Roussy ( IGR ) -Centre Hospitalier Régional Universitaire de Nancy ( CHRU Nancy ) -Université de Bourgogne ( UB ) -Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ) -Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Fédération Francophone de la Cancérologie Digestive, FFCD-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Centre National de la Recherche Scientifique ( CNRS ) -Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Université de Franche-Comté ( UFC ), Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ), Institut de Biochimie, Université de Lausanne ( UNIL ), INCa, ARC, Ligue Nationale Contre le Cancer, Swiss National Science Foundation, National Institute of Health (AI117530 and AI101423), Conseil Regional de Bourgogne, Ministère de la recherche, ANR : Labex,LipSTIC,ANR-11-LABX-0021-01-LipSTIC, ANR-07-PCVI-0031,ApoMULTI-LIB,A multivalent peptide library approach to identify functional TRAIL mimetics ( 2007 ), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, La Jolla Institute for Allergy & Immunology (LA JOLLA Institute), Nanomédecine, imagerie, thérapeutique - UFC (EA 4662) (NIT / NANOMEDECINE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Franche-Comté (UFC), Apoptosis Research Centre [Galway, Ireland] (ARC), National University of Ireland [Galway] (NUI Galway), Ghent University [Belgium] (UGENT), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université Sciences et Technologies - Bordeaux 1-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer (LabEx LipSTIC), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Paris-Sud - Paris 11 (UP11)-École pratique des hautes études (EPHE)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc (CRLCC - CGFL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-France-Comté] (EFS [Bourgogne-France-Comté])-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Université de Franche-Comté (UFC)-Université de Montpellier (UM), Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc (CRLCC - CGFL), Université de Lausanne (UNIL), ANR: 11-LABX-0021,Lipstic,Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du(2011), ANR-07-PCVI-0031,ApoMULTI-LIB,A multivalent peptide library approach to identify functional TRAIL mimetics(2007), La Jolla Institute for Immunology [La Jolla, CA, États-Unis], Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Universiteit Gent = Ghent University [Belgium] (UGENT), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Paris-Sud - Paris 11 (UP11)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Montpellier (UM), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, and ANR-11-LABX-0021,Lipstic,Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du(2011)
Subjects: TRAIL-R2, TNF, apoptosis, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, TRAIL, DR4, TRAIL-R1, N-glycosylation, DR5, signaling, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Abstract: International audience; APO2L/TRAIL (TNF-related apoptosis-inducing ligand) induces death of tumor cells through two agonist receptors, TRAIL-R1 and TRAIL-R2. We demonstrate here that N-linked glycosylation (N-glyc) plays also an important regulatory role for TRAIL-R1-mediated and mouse TRAIL receptor (mTRAIL-R)-mediated apoptosis, but not for TRAIL-R2, which is devoid of N-glycans. Cells expressing N-glyc-defective mutants of TRAIL-R1 and mouse TRAIL-R were less sensitive to TRAIL than their wild-type counterparts. Defective apoptotic signaling by N-glyc-deficient TRAIL receptors was associated with lower TRAIL receptor aggregation and reduced DISC formation, but not with reduced TRAIL-binding affinity. Our results also indicate that TRAIL receptor N-glyc impacts immune evasion strategies. The cytomegalovirus (CMV) UL141 protein, which restricts cell-surface expression of human TRAIL death receptors, binds with significant higher affinity TRAIL-R1 lacking N-glyc, suggesting that this sugar modification may have evolved as a counterstrategy to prevent receptor inhibition by UL141. Altogether our findings demonstrate that N-glyc of TRAIL-R1 promotes TRAIL signaling and restricts virus-mediated inhibition.
Published: 2017

36. N-glycosylation of mouse TRAIL-R and human TRAIL-R1 enhances TRAIL-induced death

Author: Dufour, Florent, Rattier, Thibault, Shirley, Sarah, Picarda, Gaelle, Constantinescu, Andrei Alexandru, Morlé, Aymeric, Zakaria, Al Batoul, Marcion, Guillaume, Causse, Sebastien, Szegezdi, Eva, Zajonc, Dirk Michael, Seigneuric, Renaud, Guichard, Gilles, Gharbi, Tijani, Picaud, Fabien, Herlem, Guillaume, Garrido, Carmen, Schneider, Pascal, Benedict, Chris Alan, Micheau, Olivier, Micheau, Olivier, Laboratoires d'excellence - Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du cancer - - Lipstic2011 - ANR-11-LABX-0021 - LABX - VALID, Physique et chimie du vivant - A multivalent peptide library approach to identify functional TRAIL mimetics - - ApoMULTI-LIB2007 - ANR-07-PCVI-0031 - PCVI - VALID, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Equipe HSPpathies (LNC - U1231), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, La Jolla Institute for Immunology [La Jolla, CA, États-Unis], Nanomédecine, imagerie, thérapeutique - UFC (UR 4662) (NIT / NANOMEDECINE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Apoptosis Research Centre [Galway, Ireland] (ARC), National University of Ireland [Galway] (NUI Galway), Universiteit Gent = Ghent University (UGENT), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer (LabEx LipSTIC), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Paris-Sud - Paris 11 (UP11)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-Université de Montpellier (UM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, Institut de Biochimie, Université de Lausanne = University of Lausanne (UNIL), INCa, ARC, Ligue Nationale Contre le Cancer, Swiss National Science Foundation, National Institute of Health (AI117530 and AI101423), Conseil Regional de Bourgogne, Ministère de la recherche, ANR-11-LABX-0021,Lipstic,Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du cancer(2011), and ANR-07-PCVI-0031,ApoMULTI-LIB,A multivalent peptide library approach to identify functional TRAIL mimetics(2007)
Subjects: TRAIL-R2, apoptosis, TNF, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, TRAIL, DR4, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, N-glycosylation, DR5, TRAIL-R1, signaling
Abstract: International audience; APO2L/TRAIL (TNF-related apoptosis-inducing ligand) induces death of tumor cells through two agonist receptors, TRAIL-R1 and TRAIL-R2. We demonstrate here that N-linked glycosylation (N-glyc) plays also an important regulatory role for TRAIL-R1-mediated and mouse TRAIL receptor (mTRAIL-R)-mediated apoptosis, but not for TRAIL-R2, which is devoid of N-glycans. Cells expressing N-glyc-defective mutants of TRAIL-R1 and mouse TRAIL-R were less sensitive to TRAIL than their wild-type counterparts. Defective apoptotic signaling by N-glyc-deficient TRAIL receptors was associated with lower TRAIL receptor aggregation and reduced DISC formation, but not with reduced TRAIL-binding affinity. Our results also indicate that TRAIL receptor N-glyc impacts immune evasion strategies. The cytomegalovirus (CMV) UL141 protein, which restricts cell-surface expression of human TRAIL death receptors, binds with significant higher affinity TRAIL-R1 lacking N-glyc, suggesting that this sugar modification may have evolved as a counterstrategy to prevent receptor inhibition by UL141. Altogether our findings demonstrate that N-glyc of TRAIL-R1 promotes TRAIL signaling and restricts virus-mediated inhibition.
Published: 2017

37. Identidad en el exilio. Análisis de Transit de Anna Seghers

Author: Rojas Garrido, Carmen and Palma Ceballos, Miriam
Abstract: Universidad de Sevilla. Grado en Lengua y Literaturas Alemanas
Published: 2017

38. El Bosco: el proceso creativo de sus pinturas

Author: Garrido, Carmen
Subjects: técnicas, supports, dibujo subyacente, Bosch, material analysis, El Bosco, proceso creativo, reflectografía infrarroja, radiografías, análisis de materiales, soportes, tratamientos pictóricos, underdrawing, X-ray, techniques, creative process, infrared reflectography, painting process
Abstract: De todos es conocida la importancia de la colección de las pinturas de El Bosco que se conservan en el Museo del Prado, atesoradas en su mayoría por el rey Felipe II. El estudio técnico de los tres trípticos, La Adoración de los Magos, El Carro de Heno y El Jardín de las Delicias, junto con La Mesa de los Pecados Mortales y otras de menor tamaño, nos ha permitido conocer el proceso creativo del artista, desde los soportes hasta el dibujo subyacente a la sucesión de capas de color empleadas por el artista. Con ello se pueden distinguir las obras originales de las que fueron hechas por su taller y seguidores., The significance of the collection of paintings by Bosch held in the Museo del Prado, most of them acquired by King Philip II, is widely known. Technical study of the three triptychs, The Adoration of the Magi, The Haywain and The Garden of Earthly Delights, as well as that of The Table of the Seven Deadly Sins and other smaller works, gives us some insight into Bosch’s creative process, from the supports used and the under-drawings to the successive layers of colour applied by the artist. With this information it is possible to distinguish his original works from those completed by his studio and protégées.
Published: 2016

39. Probiotic Lactobacillus Strains Activities on Autophagy and Inflammatory Response

Author: Al-Azzaz, Jana, Rieu, Aurélie, Aoudia, Nabil, Ducreux, Amandine, Jego, Gaëtan, Garrido, Carmen, DALLE, FREDERIC, Lapaquette, Pierre, Guzzo, Jean, Procédés Alimentaires et Microbiologiques (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Laboratoire de parasitologie mycologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Procédés Alimentaires et Microbiologiques ( PAM ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon )
Subjects: Gastroenterology & Hepatology, viruses, education, [ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, ComputingMilieux_MISCELLANEOUS
Abstract: International audience; The aim of the study was to evaluate the effects of probiotic strains metabolites on the reproduction of herpes simplex virus type-1 (HSV1).
Published: 2016

40. P105 Deglycosylated bleomycin has the antitumor activity of bleomycin without pulmonary toxicity

Author: Burgy , Olivier, Wettstein , Guillaume, Bellaye , Pierre-Simon, Decologne , Nathalie, Racoeur , Cindy, Goirand , Françoise, Beltramo , Guillaume, Hernandez , Jean-Francois, Kenani , Abderraouf, Camus , Philippe, Bettaieb , Ali, Garrido , Carmen, Bonniaud , Philippe, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Laboratoire d'Immunologie et Immunothérapie des Cancers ( LIIC ), École pratique des hautes études ( EPHE ) -Université de Bourgogne ( UB ), Université de Montpellier ( UM ), Faculté de Médecine de Monastir [Tunisie], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Service de Pneumologie Soins Intensifs, Appareillage Respiratoire [CHU de Dijon], Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Laboratoire d'Immunologie et Immunothérapie des Cancers (LIIC), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Bourgogne (UB), Université de Montpellier (UM), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)
Subjects: carbohydrates (lipids), bleomycin, respiratory system, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, pulmonary toxicity, [ SDV.MHEP.PSR ] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, respiratory tract diseases
Abstract: IF 2.824; International audience; Bleomycin is an anti-cancer drug used to treat different malignancies, mainly lymphomas, germ cell tumors. Unfortunately, bleomycin has major, pulmonary toxicity that affects 20% of treated individuals with pulmonary fibrosis being the most devastating form. Deglyco-bleomycin is a molecule derived from bleomycin in which the sugar residue D-mannosyl-L-glucose disaccharide has been deleted. The objective of this study is to assess the anti-cancerous activity and the lung toxicity of deglyco-bleomycin.We compared in vivo the antitumor activity in three rodent models after intraperitoneal administrated deglyco-bleomycin and bleomycin. Pulmonary toxicity was in depth examined after intratracheal administration of both chemotherapeutic agents.We demonstrate in vivo in rodent cancer models, including a human Hodgkin lymphoma xenograft and a syngeneic melanoma model, that intra-peritoneal deglyco-bleomycin is as effective as bleomycin...
Published: 2016

41. Influence of portal vein/superior mesenteric vein resection on morbility, mortality and survival of patients with pancreatic ductal adenocarcinoma in the Balearic Islands

Author: Morales Soriano, Rafael, Rodríguez Pino, José Carlos, De Juan, Carmen, Garrido, Carmen, Amengual Antich, Isabel, Guillot Morales, Mónica, Morón Canis, José M., Molina Romero, Xavier, González Argente, Xavier, and Tejada Gavela, Silvia
Subjects: Pancreatitis, Health Sciences
Abstract: Introduction: Recent developments have enabled associate to standard pancreaticoduodenectomy (DPC), vascular resections to increase resectability in pancreatic cancer. Objectives: Analyze morbidity, mortality and survival of a consecutive series of patients with pancreatic cancer, in which a DPC with portal vein resection was performed, and compared it with a group of patients with standard DPC without venous resection. Methodology: Consecutive series of 67 patients who underwent a DPC ought to pancreatic ductal adenocarcinoma, between January 2005 and January 2015. Results: Standard resection (RV-) was performed in 49 cases, and a venous resection in another 18 patients (RV+). There were no significant differences in age (65 vs 68.9 years), ASA, or intraoperative transfusion. Duration of intervention was significantly lower in the RV- group (6.1 vs 6.7; p = 0.05). Morbidity grade III -IV was 14.2 % Clavien in the RV- group and 16.6 % in the RV + group (p = 0.87). There were no differences in hospital mortality (0 % vs 5.5%), or hospital stay (14.4 vs 15.2 days). The surgical margin involvement was more frequent in the RV+ group (18 % vs 50 % ; p = 0.003). One, 3 and 5 years survival was 77, 34 and 11% in the RV- group and 92, 23 and 8% in the group with venous resection. Conclusions: DPC with venous resection can be performed with morbidity and mortality rates similar to standard DPC1. Survival shows no significant difference between the two groups.Venous resection may increase resectability in a selected group of patients with pancreatic adenocarcinoma. Introducción: Los progresos recientes han permitido asociar a la duodenopancreatectomía cefálica estándar (DPC), resecciones vasculares para incrementar la resecabilidad en el cáncer de páncreas. Objetivos: Analizar la morbi-mortalidad y supervivencia de una serie consecutiva de pacientes con cáncer de páncreas, en los que se realizó una DPC con resección de vena porta y compararla con un grupo de pacientes con DPC estándar sin resección venosa. Material y métodos: Serie consecutiva de 67 pacientes intervenidos con adenocarcinoma ductal de páncreas, entre enero 2005 y enero 2015. Resultados: En 49 casos se realizó una resección estándar (RV-) y en 18 pacientes, una resección venosa (RV+). No hubo diferencias significativas en la edad (65 vs 68,9 años), ASA, ni en la transfusión intraoperatoria. La duración de la intervención fue significativamente menor en el grupo RV- (6,1 vs 6,7; p= 0,05). La morbilidad grado III-IV de Clavien fue del 14,2% en el grupo RV- y del 16,6% en el grupo RV+ (p=0,87). No hubo diferencias en la mortalidad hospitalaria (0% vs 5,5%), ni en la estancia hospitalaria (14,4 vs 15,2 días). La afectación del margen quirúrgico fue más frecuente en el grupo RV+ (18% vs 50%; p=0,003). La supervivencia al año, 3 y 5 años fue del 77, 34 y 11% en el grupo RV-,y del 92, 23 y 8% en el grupo con resección venosa. Conclusiones: La DPC con resección venosa puede realizarse con tasas de morbi-mortalidad similares a la DPC estándar. La supervivencia no muestra diferencias significativas entre los dos grupos. La resección venosa puede aumentar la resecabilidad en un grupo seleccionado de pacientes con adenocarcinoma de páncreas.
Published: 2016

42. Identificación y análisis de una problemática en un aula de 6º curso de Educación Primaria

Author: Cruz Garrido, Carmen and Serrano Soriano, Ester
Subjects: comportamiento y cambios, intervención, primaria
Abstract: En este documento se propone la intervención y el análisis dentro de un aula de sexto curso de Educación Primaria con la ayuda del tutor de dicho curso y el orientador del centro. El caso práctico a intervenir es una clase donde se presentan problemas de comportamiento debido a los cambios que se están produciendo en su vida, el inicio de la adolescencia. Esta intervención se va a llevar a cabo a través de una serie de actividades que les ayuden a comprender esta etapa y mejorar sus rendimientos académicos. In this document one proposes the intervention and the analysis inside a classroom of sixth course of Primary Education with the help of the tutor of the above mentioned course and the other teacher of the center. The practical case to intervening is a class where they present problems of behavior due to the changes that are taking place in his life, the beginning of the adolescence. This intervention is going to carried out across a series of activities that help them to understand this stage and to improve his academic performances.
Published: 2016

43. Influencia de la resección de vena porta/vena mesentérica superior en la morbilidad, mortalidad y supervivencia de los pacientes con adenocarcinoma ductal de páncreas en las Islas Baleares

Author: Morales Soriano, Rafael, Rodríguez Pino, José Carlos, De Juan, Carmen, Garrido, Carmen, Amengual Antich, Isabel, Guillot Morales, Mónica, Morón Canis, José M., Molina Romero, Xavier, González Argente, Xavier, and Tejada Gavela, Silvia
Subjects: Pancreatitis, Health Sciences
Abstract: Introduction: Recent developments have enabled associate to standard pancreaticoduodenectomy (DPC), vascular resections to increase resectability in pancreatic cancer. Objectives: Analyze morbidity, mortality and survival of a consecutive series of patients with pancreatic cancer, in which a DPC with portal vein resection was performed, and compared it with a group of patients with standard DPC without venous resection. Methodology: Consecutive series of 67 patients who underwent a DPC ought to pancreatic ductal adenocarcinoma, between January 2005 and January 2015. Results: Standard resection (RV-) was performed in 49 cases, and a venous resection in another 18 patients (RV+). There were no significant differences in age (65 vs 68.9 years), ASA, or intraoperative transfusion. Duration of intervention was significantly lower in the RV- group (6.1 vs 6.7; p = 0.05). Morbidity grade III -IV was 14.2 % Clavien in the RV- group and 16.6 % in the RV + group (p = 0.87). There were no differences in hospital mortality (0 % vs 5.5%), or hospital stay (14.4 vs 15.2 days). The surgical margin involvement was more frequent in the RV+ group (18 % vs 50 % ; p = 0.003). One, 3 and 5 years survival was 77, 34 and 11% in the RV- group and 92, 23 and 8% in the group with venous resection. Conclusions: DPC with venous resection can be performed with morbidity and mortality rates similar to standard DPC1. Survival shows no significant difference between the two groups.Venous resection may increase resectability in a selected group of patients with pancreatic adenocarcinoma., Introducción: Los progresos recientes han permitido asociar a la duodenopancreatectomía cefálica estándar (DPC), resecciones vasculares para incrementar la resecabilidad en el cáncer de páncreas. Objetivos: Analizar la morbi-mortalidad y supervivencia de una serie consecutiva de pacientes con cáncer de páncreas, en los que se realizó una DPC con resección de vena porta y compararla con un grupo de pacientes con DPC estándar sin resección venosa. Material y métodos: Serie consecutiva de 67 pacientes intervenidos con adenocarcinoma ductal de páncreas, entre enero 2005 y enero 2015. Resultados: En 49 casos se realizó una resección estándar (RV-) y en 18 pacientes, una resección venosa (RV+). No hubo diferencias significativas en la edad (65 vs 68,9 años), ASA, ni en la transfusión intraoperatoria. La duración de la intervención fue significativamente menor en el grupo RV- (6,1 vs 6,7; p= 0,05). La morbilidad grado III-IV de Clavien fue del 14,2% en el grupo RV- y del 16,6% en el grupo RV+ (p=0,87). No hubo diferencias en la mortalidad hospitalaria (0% vs 5,5%), ni en la estancia hospitalaria (14,4 vs 15,2 días). La afectación del margen quirúrgico fue más frecuente en el grupo RV+ (18% vs 50%; p=0,003). La supervivencia al año, 3 y 5 años fue del 77, 34 y 11% en el grupo RV-,y del 92, 23 y 8% en el grupo con resección venosa. Conclusiones: La DPC con resección venosa puede realizarse con tasas de morbi-mortalidad similares a la DPC estándar. La supervivencia no muestra diferencias significativas entre los dos grupos. La resección venosa puede aumentar la resecabilidad en un grupo seleccionado de pacientes con adenocarcinoma de páncreas.
Published: 2016

44. Dose-dependent biphasic leptin-induced proliferation is caused by non-specific IL-6/NF-κB pathway activation in human myometrial cells

Author: Barrichon, Marina, Hadi, Tarik, Wendremaire, Maeva, Ptasinski, Clémentine, Seigneuric, Renaud, Marcion, Guillaume, Delignette, Marc, Marchet, Jacques, Dumas, Monique, Sagot, Paul, Bardou, Marc, Garrido, Carmen, and Lirussi, Frédéric
Subjects: Leptin, STAT3 Transcription Factor, Dose-Response Relationship, Drug, Interleukin-6, Myocytes, Smooth Muscle, NF-kappa B, Research Papers, Pregnancy, Myometrium, Humans, Receptors, Leptin, Female, Cell Proliferation, Signal Transduction
Abstract: Leptin, an adipokine synthesized by the placenta during pregnancy, has been proposed for the management of preterm labour (PTL), as it is able to prevent in vitro uterine contractility and remodelling associated with labour onset. Another common feature of labour onset is the phenotypic switch of myometrial smooth muscle cells from a proliferative to a hypertrophic state. As proliferative effects have been demonstrated for leptin in other tissues, we aimed to investigate its ability to induce myometrial proliferation and thus to maintain uterine quiescence.We stimulated human primary myometrial smooth muscle cells with leptin in the presence or absence of receptor antagonists or signalling pathway inhibitors.Leptin induced myometrial cell proliferation in a biphasic manner. At 6.25 ng · mL(-1), leptin-induced proliferation was mediated by the leptin receptor and required the early activation of ERK1/2. At a concentration above 25 ng · mL(-1), leptin induced direct non-specific stimulation of the IL-6 receptor, leading to NF-κB activation, and exerted anti-proliferative effects. However, at 50 ng · mL(-1), leptin re-induces proliferation via IL-6 receptor stimulation that requires STAT3 and delayed ERK1/2 activation.These data bring new insights into leptin signalling-induced myometrial proliferation and its interrelationship with the IL-6/IL-6 receptor axis. In the light of our previous work, the present study emphasizes the potential value of leptin in the pharmacological management of PTL and it also strengthens the hypothesis that leptin might be a contributory factor in the parturition-related disorders observed in obese women.
Published: 2015

45. HSP90 and HSP70: Implication in Inflammation Processes and Therapeutic Approaches for Myeloproliferative Neoplasms

Author: Sevin, Margaux, Girodon, François, Garrido, Carmen, and de Thonel, Aurélie
Subjects: Article Subject
Abstract: Myeloproliferative neoplasms (MPN) are clonal stem cell disorders that lead to the excessive production of one or more blood cell lineages. It has been reported that, in most MPN, inflammatory cytokines are frequently increased, indicating that inflammation plays a crucial role in these disorders. Heat shock proteins (HSP) are induced in response to many stressful conditions from heat shock to hypoxia and inflammation. Besides their chaperone and cytoprotective functions, HSPs are key players during inflammation, hence the term “chaperokine.” Through their chaperone activity, HSP90, a stabilizer of many oncogenes (e.g., JAK2), and HSP70, a powerful antiapoptotic chaperone, tightly regulate Nuclear Factor-kappa B signalling, a critical pathway in mediating inflammatory responses. In light of this potential, several HSP90 inhibitors have been generated as anticancer agents able to degrade oncogenes. As it turns out, however, these drugs are also potent inhibitors of the inflammatory response in various diseases. Given the chaperone potential of HSP70 and the fact that HSP90 inhibitors induce HSP70, interest in HSP70 inhibitors is also increasing. Here, we focus on the implication of HSP90 and HSP70 in inflammatory responses and on the emergence of new therapeutic approaches in MPN based on HSP inhibitors.
Published: 2015
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46. La belleza de la manada

Author: Garrido, Carmen
Subjects: Imágenes - Interpretación
Published: 2015

47. Mecanismos moleculares asociados al envejecimiento

Author: Escalona Garrido, Carmen, Montoro García, Blanca, and Iniesta Serrano, Pilar
Subjects: Geriatría, Biología molecular, Farmacología
Abstract: El envejecimiento es una situación que preocupa a la gran mayoría de la población y cuya etiología es bastante compleja. Hemos realizado una revisión bibliográfica de artículos que estudian los principales mecanismos moleculares implicados en este proceso con independencia de que los resultados fueran aplicables a animales o humanos. Los principales marcadores asociados al envejecimiento identificados hasta el momento son: inestabilidad genómica, desgaste telomérico, pérdida de proteostasis, desregulación de los sistemas de detección de nutrientes, alteraciones epigenéticas, disfunción mitocondrial, senescencia celular, agotamiento de células madre y alteración de la comunicación celular.
Published: 2015

48. Modelo de Seguridad en la Nube utilizando Criptografía de Curva Elíptica

Author: Espinosa Garrido, Carmen Beatriz and Dr. Vicente Alarcón Aquino
Subjects: 5 CIENCIAS SOCIALES, Ciencias de la Computación
Abstract: El objetivo de este trabajo consiste en presentar un modelo de seguridad en la nube pública utilizando la criptografía de la curva elíptica que incremente los niveles de confianza bajo los cuales actualmente operan las redes de comunicaciones en la nube computacional. Asimismo, proponer un esquema que evite que la información que pasa por una nube computacional pública se vea comprometida ante la presencia de un intruso. La seguridad en las redes computaciones es sin duda un tema importante hoy en día y el debate público suele ir acompañado de una gran cantidad de expectativa, utilizando en las notas de prensa el término irrompible para los criptosistemas con grandes llaves. Sin embargo, la demostración de que tan fuerte es un esquema de cifrado a menudo falla en la medición de la fuerza real. Los temas de seguridad que intenta mitigar el modelo propuesto de seguridad son los siguientes: privacidad, los usuarios necesitan que sus datos sean transmitidos, de manera que sean ocultados para cualquier tercero que monitoree las comunicaciones. Identidad de usuarios, donde ambas partes que comunican información deben de estar confiadas de la identidad de los otros. Integridad de mensajes, que los usuarios que se comunican a través de una red, estén seguros de que la otra parte no ha modificado maliciosamente los datos transmitidos. Para la seguridad en la transmisión de los datos dentro de este modelo se manipulará la criptografía de la curva elíptica, la cual cuenta con ventajas claramente definidas en comparación con RSA. En cuanto a la rapidez, tamaño, y la propia seguridad. La criptografía de la curva elíptica tiene un procesamiento más rápido, utiliza menos memoria y almacenamientos en los dispositivos y ofrece seguridad en datos de igual o mayor nivel que el algoritmo RSA. La criptografía de curva elíptica (ECC) es una criptografía de llave pública, esto quiere decir: que cada usuario o dispositivo participa en la comunicación utilizando una llave pública y otra privada y un conjunto de operaciones asociadas a estas llaves que son usadas en el procesamiento criptográfico. Sólo el usuario en particular conoce la llave privada mientras que la llave pública se distribuye a todos los usuarios que participan en la comunicación. Algunos algoritmos de llave pública pueden requerir un conjunto de constantes predefinidas para ser conocidos por todos los dispositivos que participan en la comunicación. Los "Parámetros de dominio" en ECC es un ejemplo de tales constantes. La criptografía de llave pública, a diferencia de la criptografía de llave privada, no requiere ninguna llave secreta compartida entre las partes que se comunican, pero es mucho más lenta que la criptografía de llave privada. La seguridad de ECC depende de la dificultad de resolver el Problema de Logaritmo Discreto de la Curva. Al cotejar el procesamiento de los algoritmos de RSA y ECC se obtiene un mejor desempeño, reducción de tiempo y alto nivel de seguridad al cifrar datos con la Curva Elíptica.
Published: 2014

49. The biofilm mode of life boosts the anti-inflammatory properties of L actobacillus

Author: Rieu, Aurélie, Aoudia, Nabil, Jego, Gaëtan, Chluba, Johanna, Yousfi, Nadhir, Briandet, Romain, Deschamps, Julien, Gasquet, Benoît, Monedero, Vicente, Garrido, Carmen, Guzzo, Jean, and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: [SDV]Life Sciences [q-bio]
Published: 2014

50. The biofilm mode of life boosts the anti-inflammatory properties of Lactobacillus

Author: Rieu, Aurelie, Aoudia, Nabil, Jego, Gaetan, Chluba, Johanna, Yousfi, Nadhir, Briandet, Romain, Deschamps, Julien, Gasquet, Benoit, Monedero, Vicente, Garrido, Carmen, Guzzo, Jean, Procédés Alimentaires et Microbiologiques (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Lipides - Nutrition - Cancer (U866) (LNC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Plateforme d'Imagerie Cellulaire [Dijon] (CellImaP), Université de Bourgogne (UB)-IFR100 - Structure fédérative de recherche Santé-STIC, Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de Alimentos, Spanish National Research Council (CSIC), Centre anticancéreux, Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER, Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc (CRLCC - CGFL), Procédés Alimentaires et Microbiologiques ( PAM ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), MICrobiologie de l'ALImentation au Service de la Santé humaine ( MICALIS ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Plateforme d'Imagerie Cellulaire [Dijon] ( CellImaP ), Université de Bourgogne ( UB ) -IFR100 - Structure fédérative de recherche Santé-STIC, Spanish National Research Council ( CSIC ), and Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL )
Subjects: PROBIOTIC ACTION, SULFONIC-ACID, SOLUBLE-PROTEIN, [ SDV ] Life Sciences [q-bio], HELICOBACTER-PYLORI, ZEBRAFISH, LACTIC-ACBACTERIA, [SDV]Life Sciences [q-bio], NF-KAPPA-B, biochemical phenomena, metabolism, and nutrition, IN-VIVO, CHAPERONIN GROEL, INFLAMMATORY-BOWEL-DISEASE
Abstract: The predominant form of life for microorganisms in their natural habitats is the biofilm mode of growth. The adherence and colonization of probiotic bacteria are considered as essential factors for their immunoregulatory function in the host. Here, we show that Lactobacillus caseiATCC334 adheres to and colonizes the gut of zebrafish larvae. The abundance of pro-inflammatory cytokines and the recruitment of macrophages were low when inflammation was induced in probiotic-fed animals, suggesting that these bacteria have anti-inflammatory properties. We treated human macrophage-differentiated monocytic THP-1 cells with supernatants of L.caseiATCC334 grown in either biofilm or planktonic cultures. TNF- production was suppressed and the NF-B pathway was inhibited only in the presence of supernatants from biofilms. We identified GroEL as the biofilm supernatant compound responsible, at least partially, for this anti-inflammatory effect. Gradual immunodepletion of GroEL demonstrated that the abundance of GroEL and TNF- were inversely correlated. We confirmed that biofilm development in other Lactobacillus species affects the immune response. The biofilms supernatants of these species also contained large amounts of GroEL. Thus, our results demonstrate that the biofilm enhances the immunomodulatory effects of Lactobacillus sp. and that secreted GroEL is involved in this beneficial effect.
Published: 2014

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