1. A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current Chromatography
- Author
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Geng Yanling, Xiao Wang, Hongjing Dong, Ruixuan Zhao, Jiang Jiaojiao, Yan Mu, Zhenjia Zheng, and Wang Tao
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Magnetic Resonance Spectroscopy ,Pharmaceutical Science ,01 natural sciences ,Justicia procumbens ,Article ,Lignans ,Justicia procumbens L ,lignans and their glycosides ,preparative separation ,high-speed counter-current chromatography ,Analytical Chemistry ,lcsh:QD241-441 ,chemistry.chemical_compound ,Countercurrent chromatography ,lcsh:Organic chemistry ,Justicia ,Drug Discovery ,Diphyllin acetyl apioside ,Physical and Theoretical Chemistry ,Chromatography, High Pressure Liquid ,Solvent system ,chemistry.chemical_classification ,Lignan ,Chromatography ,Molecular Structure ,biology ,010405 organic chemistry ,010401 analytical chemistry ,Organic Chemistry ,Glycoside ,biology.organism_classification ,0104 chemical sciences ,chemistry ,Chemistry (miscellaneous) ,Solvents ,Molecular Medicine - Abstract
Ten compounds, including three lignan glycosides and seven lignans, were purified from Justicia procumbens L. in 8 h using an efficient strategy based on high-speed counter-current chromatography (HSCCC). The two-phase solvent system composed of petroleum–ethyl acetate–methanol–H2O (1:0.7:1:0.7, v/v) was firstly employed to separate the crude extract (320 mg), from which 19.3 mg of justicidin B (f), 10.8 mg of justicidin A (g), 13.9 mg of 6′-hydroxyjusticidin C (h), 7.7 mg of justicidin E (i), 6.3 mg of lignan J1 (j) were obtained with 91.3 mg of enriched mixture of compounds a–e. The enriched mixture (91.3 mg) was further separated using the solvent system consisting of petroleum–ethyl acetate–methanol–H2O (3:3.8:3:3.8, v/v), yielding 12.1 mg of procumbenoside E (a); 7.6 mg of diphyllin-1-O-β-d-apiofuranoside (b); 7.4 mg of diphyllin (c); 8.3 mg of 6′-hydroxy justicidin B (d); and 7.9 mg of diphyllin acetyl apioside (e). The purities of the 10 components were all above 94%, and their structures were identified by NMR and ESI-MS spectra. The results demonstrated that the strategy based on HSCCC for the separation of lignans and their glycosides was efficient and rapid.
- Published
- 2017
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