8 results on '"Haolin Fang"'
Search Results
2. A custom-made time-resolved fluoroimmunoassay for the quantitation of the host cell protein of Vero in rabies vaccine
- Author
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Yiqi Yang, Zhaoyue Li, Zhigao Zhang, Xiangming Zhai, Xijiu Li, Yue Cao, Haolin Fang, Chunhui He, Yingsong Wu, and Guanfeng Lin
- Subjects
Virology - Published
- 2023
3. Driving–Charging Integrated Controller for Electric Vehicles
- Author
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Kai Zhou, Haolin Fang, and Yang Liu
- Subjects
General Computer Science ,General Engineering ,General Materials Science ,Electrical and Electronic Engineering - Published
- 2022
4. A time-resolved fluoroimmunoassay for assessing rabies antibody titers in the sera of vaccinated human subjects
- Author
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Haolin Fang, Ke Ye, Lun Bian, Guanfeng Lin, Chunhui He, Zhenhua Chen, Li Lin, Hui Zhao, and Ying-Song Wu
- Subjects
0301 basic medicine ,Time Factors ,Rabies ,Fluoroimmunoassay ,Enzyme-Linked Immunosorbent Assay ,Bioengineering ,Antibodies, Viral ,Sensitivity and Specificity ,Applied Microbiology and Biotechnology ,Rabies vaccination ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,030212 general & internal medicine ,Pharmacology ,General Immunology and Microbiology ,business.industry ,Vaccination ,Antibody titer ,Reproducibility of Results ,General Medicine ,Serum samples ,medicine.disease ,Virology ,Titer ,030104 developmental biology ,Rabies Vaccines ,Rabies virus ,business ,Biotechnology - Abstract
Several studies have investigated the use of simple in vitro tests for the assessment of rabies antibody titers in serum samples from vaccinated human subjects, which would allow the effectiveness of rabies vaccination to be conveniently evaluated. To this end, a novel time-resolved fluoroimmunoassay (TRFIA) for the assessment of rabies antibody titers was established in this study for evaluating the effectiveness of protection against rabies. The TRFIA had a satisfactory limit of detection value (0.035 IU/mL) under optimal conditions. Additionally, the application of the TRFIA was demonstrated in 68 serum samples with satisfactory results. The coefficient variations (CVs) were all
- Published
- 2020
5. Neural adaptive output feedback tracking control of underactuated AUVs
- Author
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Kai Fang, Jiawang Li, Jiaqi Yao, Haolin Fang, and Jiawen Zhang
- Subjects
Computer Science::Robotics ,Environmental Engineering ,Observer (quantum physics) ,Artificial neural network ,Underactuation ,Computer science ,Control theory ,Backstepping ,Bounded function ,Trajectory ,Ocean Engineering ,Kinematics ,Parametric statistics - Abstract
This paper addresses the three-dimensional tracking and stabilization problems simultaneously for underactuated autonomous underwater vehicles (AUVs) subjected to parametric uncertainties, external disturbances, unmeasured states and input saturation. A modified error state formulation is introduced to produce auxiliary virtual controls for the underactuated kinematics. A new high-gain observer is proposed to estimate unavailable velocities which doesn't need the position and orientation of AUVs to be bounded. Based on the backstepping technique and combinations of high-gain observer and radial-basis-function neural network (RBFNN) approximations, the kinematic and dynamic controllers are designed to force underactuated AUVs to track a time-varying trajectory or/and a stationary point, and guarantees the tracking errors convergent to a bounded neighborhood of the origin. Some comparative simulations are presented to validate the effectiveness and performance of the proposed control scheme.
- Published
- 2021
6. Mechano growth factor attenuates mechanical overload-induced nucleus pulposus cell apoptosis through inhibiting the p38 MAPK pathway
- Author
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Baofang Tian, Qing Xu, Luo Zhang, Haolin Fang, Liang Zhao, and Cunxin Zhang
- Subjects
0301 basic medicine ,Mechanical overload ,Male ,Nucleus Pulposus ,MAP Kinase Signaling System ,Poly ADP ribose polymerase ,p38 mitogen-activated protein kinases ,Biophysics ,Gene Expression ,Peptide ,Biochemistry ,p38 Mitogen-Activated Protein Kinases ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Gene expression ,medicine ,Animals ,Insulin-Like Growth Factor I ,Growth Substances ,Molecular Biology ,Gene ,Research Articles ,Cells, Cultured ,chemistry.chemical_classification ,mechano growth factor ,intervertebral disc degeneration ,apoptosis ,Cell Biology ,Cell biology ,mechanical overload ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Apoptosis ,030220 oncology & carcinogenesis ,Female ,Stress, Mechanical ,Apoptosis Regulatory Proteins ,Nucleus ,Research Article - Abstract
Mechanical overload is a risk factor of disc degeneration. It can induce disc degeneration through mediating cell apoptosis. Mechano growth factor (MGF) has been reported to inhibit mechanical overload-induced apoptosis of chondrocytes. The present study is aimed to investigate whether MGF can attenuate mechanical overload-induced nucleus pulposus (NP) cell apoptosis and the possible signaling transduction pathway. Rat NP cells were cultured and subjected to mechanical overload for 7 days. The control NP cells did not experience mechanical load. The exogenous MGF peptide was added into the culture medium to investigate its protective effects. NP cell apoptosis ratio, caspase-3 activity, gene expression of Bcl-2, Bax and caspase-3, protein expression of cleaved caspase-3, cleaved PARP, Bax and Bcl-2 were analyzed to evaluate NP cell apoptosis. In addition, activity of the p38 MAPK pathway was also detected. Compared with the control NP cells, mechanical overload significantly increased NP cell apoptosis and caspase-3 activity, up-regulated gene/protein expression of pro-apoptosis molecules (i.e. Bax, caspase-3, cleaved caspase-3 and cleaved PARP) whereas down-regulated gene/protein expression of anti-apoptosis molecule (i.e. Bcl-2). However, exogenous MGF partly reversed these effects of mechanical overload on NP cell apoptosis. Further results showed that activity of the p38 MAPK pathway of NP cells cultured under mechanical overload was decreased by addition of MGF peptide. In conclusion, MGF is able to attenuate mechanical overload-induced NP cell apoptosis, and the p38 MAPK signaling pathway may be involved in this process. The present study provides that MGF supplementation may be a promising strategy to retard mechanical overload-induced disc degeneration.
- Published
- 2019
7. Extensive mechanical tension promotes annulus fibrosus cell senescence through suppressing cellular autophagy
- Author
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Qing Xu, Cunxin Zhang, Baofang Tian, Liang Zhao, Luo Zhang, and Haolin Fang
- Subjects
0301 basic medicine ,Senescence ,Male ,Telomerase ,autophagy ,senescence ,Cell ,Biophysics ,Intervertebral Disc Degeneration ,Matrix (biology) ,Biochemistry ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Molecular Biology ,Aggrecan ,Cells, Cultured ,Cellular Senescence ,Research Articles ,Cell Proliferation ,Cell growth ,Chemistry ,Autophagy ,Annulus Fibrosus ,annulus fibrosus cell ,Cell Biology ,Cell cycle ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Stress, Mechanical ,Research Article ,mechanical tension - Abstract
Background: Mechanical load contributes a lot to the initiation and progression of disc degeneration. Annulus fibrosus (AF) cell biology under mechanical tension remains largely unclear. Objective: The present study was aimed to investigate AF cell senescence under mechanical tension and the potential role of autophagy. Methods: Rat AF cells were cultured and experienced different magnitudes (5% elongation and 20% elongation) of mechanical tension for 12 days. Control AF cells were kept static. Cell proliferation, telomerase activity, cell cycle fraction, and expression of senescence-related molecules (p16 and p53) and matrix macromolecules (aggrecan and collagen I) were analyzed to evaluate cell senescence. In addition, expression of Beclin-1 and LC3, and the ratio of LC3-II to LC3-I were analyzed to investigate cell autophagy. Results: Compared with the control group and 5% tension group, 20% tension group significantly decreased cell proliferation potency and telomerase activity, increased G1/G0 phase fraction, and up-regulated gene/protein expression of p16 and p53, whereas down-regulated gene/protein expression of aggrecan and collagen I. In addition, autophagy-related parameters such as gene/protein expression of Beclin-1 and LC3, and the ratio of LC3-II to LC3-I, were obviously suppressed in the 20% tension group. Conclusion: High mechanical tension promotes AF cell senescence though suppressing cellular autophagy. The present study will help us to better understand AF cell biology under mechanical tension and mechanical load-related disc degeneration.
- Published
- 2019
8. Osteogenic protein-1 attenuates apoptosis and enhances matrix synthesis of nucleus pulposus cells under high magnitude compression though inhibiting the p38 MAPK pathway
- Author
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Buwei Sun, Haolin Fang, Xianzhou Li, Haijun Teng, Pei Li, and Haiming Shen
- Subjects
p38 mitogen-activated protein kinases ,Biophysics ,Matrix (biology) ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Molecular Biology ,Research Articles ,Aggrecan ,030203 arthritis & rheumatology ,TUNEL assay ,Catabolism ,Chemistry ,nucleus pulposus ,apoptosis ,Cell Biology ,compression ,matrix ,Cell biology ,medicine.anatomical_structure ,Terminal deoxynucleotidyl transferase ,Apoptosis ,Nucleus ,osteogenic protein-1 ,030217 neurology & neurosurgery ,Research Article - Abstract
Disc degeneration is correlated with mechanical load. Osteogenic protein-1 (OP-1) is potential to regenerate degenerative disc. To investigate whether OP-1 can protect against high magitude compression-induced nucleus pulposus (NP) cell apoptosis and NP matrix catabolism, and its potential mechanism; porcine discs were cultured in a bioreactor and compressed at a relatively high-magnitude mechanical compression (1.3 MPa at a frequency of 1.0 Hz for 2 h once per day) for 7 days. OP-1 was added along with the culture medium to investigate the protective effects of OP-1. NP cell apoptosis and matrix biosynthesis were evaluated. Additionally, activity of the p38 MAPK pathway is also analyzed. Compared with the control group, high magnitude compression significantly promoted NP cell apoptosis and decreased NP matrix biosynthesis, reflected by the increase in the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive cells and caspase-3 activity, the up-regulated expression of Bax and caspase-3 mRNA and down-regulated expression of Bcl-2 mRNA, and the decreased Alcian Blue staining intensity and expression of matrix proteins (aggrecan and collagen II). However, OP-1 addition partly attenuated the effects of high magnitude compression on NP cell apoptosis and NP matrix biosynthesis. Further analysis showed that inhibition of the p38 MAPK pathway partly participated in this process. OP-1 can attenuate high magnitude compression-induced NP cell apoptosis and promoted NP matrix biosynthesis, and inhibition of the p38 MAPK pathway may participate in this regulatory process. The present study provides that OP-1 may be efficient in retarding mechanical overloading-exacerbated disc degeneration.
- Published
- 2018
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