1. Cysteinyl leukotriene receptors in tonsillar B- and T-lymphocytes from children with obstructive sleep apnea
- Author
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Tsaoussoglou, M. Lianou, L. Maragozidis, P. Hatzinikolaou, S. Mavromati, M. Orologas, N. Panaghiotopoulou-Gartagani, P. Rassidakis, G. Balatsos, N.A.A. Chrousos, G. Kaditis, A.G.
- Subjects
stomatognathic system ,respiratory tract diseases - Abstract
Objectives: Cysteinyl leukotrienes have been implicated in the pathogenesis of adenotonsillar hypertrophy in children with obstructive sleep apnea (OSA). This study aimed to quantify the expression of cysteinyl leukotriene receptors (CysLT1, CysLT2) by tonsillar lymphocyte subpopulations from children with OSA and to make comparisons to lymphocyte subpopulations from control subjects with recurrent tonsillitis (RT). Methods: Tonsillar tissue from children with OSA or RT was studied for CysLT1 and CysLT2 expression by RT-PCR, flow cytometry (FC), and immunofluorescence. Results: Ten children with OSA and 10 control subjects were recruited. In OSA participants, CysLT1+ fraction of small-size CD19+ B-lymphocytes was similar to the CysLT1+ CD3+ T-lymphocytes fraction (FC: 36.5 [16.5-55.4] vs. 14 [2.8-22.1]) (p>0.05) and higher than the CysLT1+ moderate/large-size CD19+ B-lymphocytes fraction (6.6 [1.5-14.4]) (p
- Published
- 2012