1. Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots
- Author
-
Furesi, Giulia, Fert, Ingrid, Beaufrère, Marie, Araujo, Luiza M.M., Glatigny, Simon, Baschant, Ulrike, von Bonin, Malte, Hofbauer, Lorenz Christian, Horwood, Nicole J., Breban, Maxime, Rauner, M., HAL UVSQ, Équipe, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Infection et inflammation (2I), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Excellence INFLAMEX [Paris], Université Sorbonne Paris Cité (USPC), Hôpital Ambroise Paré [AP-HP], University of East Anglia [Norwich] (UEA), 860898 Horizon 2020 Framework Programme, H2020 Deutsche Forschungsgemeinschaft, DFG Versus Arthritis: 20372, This work was supported by grants from the Deutsche Forschungsgemeinschaft and the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 860898 to MR and LH, as well as NH, supported by Versus Arthritis Grant No. 20372., and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)
- Subjects
CD4-Positive T-Lymphocytes ,Mice, Inbred BALB C ,HLA-B27 transgenic rat ,[SDV]Life Sciences [q-bio] ,Immunology ,Interleukin-17 ,Mice, Transgenic ,spondyloarthritis ,Rats, Inbred F344 ,SKG mouse ,Rats ,[SDV] Life Sciences [q-bio] ,Disease Models, Animal ,Mice ,bone marrow fat ,IL-17 ,Adipose Tissue ,Bone Marrow ,Spondylarthritis ,Animals ,Female ,Rats, Transgenic ,HLA-B27 Antigen ,Original Research - Abstract
International audience; Bone marrow adipose tissue (BMAT) has recently been recognized as a distinct fat depot with endocrine functions. However, if and how it is regulated by chronic inflammation remains unknown. Here, we investigate the amount of white fat and BMAT in HLA-B27 transgenic rats and curdlan-challenged SKG mice, two well-established models of chronic inflammatory spondyloarthritis (SpA). Subcutaneous and gonadal white adipose tissue and BMAT was reduced by 65-70% and by up to 90% in both experimental models. Consistently, B27 rats had a 2-3-fold decrease in the serum concentrations of the adipocyte-derived cytokines adiponectin and leptin as well as a 2-fold lower concentration of triglycerides. The bone marrow of B27 rats was further characterized by higher numbers of neutrophils, lower numbers of erythroblast precursors, and higher numbers of IL-17 producing CD4+ T cells. IL-17 concentration was also increased in the serum of B27 rats. Using a cell culture model, we show that high levels of IL-17 in the serum of B27 rats negatively impacted adipogenesis (-76%), an effect that was reversed in the presence of neutralizing anti-IL-17 antibody. In summary, these findings show BMAT is severely reduced in two experimental models of chronic inflammatory SpA and suggest that IL-17 is involved in this process.
- Published
- 2021