1. The Inhibitory Activity of Picria fel-terrae Lour Herbs Extract on Nitric Oxide Production toward RAW 264.7 Cells Induced by Lipopolysaccharide
- Author
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Denny Satria, Sri Suryani, Novycha Auliafendri, Yuandani Yuandani, and Rosidah Rosidah
- Subjects
Lipopolysaccharide ,Ethyl acetate ,lcsh:Medicine ,030209 endocrinology & metabolism ,Herbal Medicine in Pharmaceutical and Clinical Sciences ,Picria fel-terrae Lour Herbs Extract ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Griess test ,Maceration (wine) ,Medicine ,030212 general & internal medicine ,RAW 264.7 Cells ,Ethanol ,Traditional medicine ,Immunosuppresive effects ,business.industry ,Extraction (chemistry) ,lcsh:R ,Immunosuppressive effects ,General Medicine ,Nitric Oxide production ,chemistry ,business - Abstract
AIM: The objective of this study was to evaluate the inhibitory activity of Picria fel-terrae Lour on Nitric Oxide production toward RAW 264.7 cells. METHODS: The extraction was obtained by maceration method using n-hexane, ethyl acetate and ethanol solvents and then nitric oxide (NO) production was obtained using Griess reagent. RESULTS: Extract of Picria fel-terrae Lour herbs can reduce the NO production toward RAW 264.7 cells with induced by lipopolysaccharide has obtained nitric concentrations 12.5 and 25 μg/mL from n-hexane extract (72.50 ± 4.51 and 10.42 ± 1.82), ethyl acetate extract: (88.33 ± 6.51 and 30.83 ± 6.86), ethanol extract: (75.00 ± 1.91 and 22.08 ± 2.53). CONCLUSION: n-hexane extract of Picria fel-terrae Lour Herbs has a high potential to reduce the NO production in LPS-stimulated RAW 264.7 cells compared to ethyl acetate and ethanol extracts of Picria fel-terrae Lour Herbs.
- Published
- 2019