1. Shavenbaby and Yorkie mediate Hippo signaling to protect adult stem cells from apoptosis
- Author
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Bohère, Jérôme, Mancheno-Ferris, Alexandra, Al Hayek, Sandy, Zanet, Jennifer, Valenti, Philippe, Akino, Kohsuke, Yamabe, Yuya, Inagaki, Sachi, Chanut-Delalande, Hélène, Plaza, Serge, Kageyama, Yuji, Osman, Dani, Polesello, Cédric, Payre, François, Centre de biologie du développement (CBD), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Parisien de l'Epaule, and Kanazawa Institute of Technology
- Subjects
animal structures ,Science ,Intracellular Signaling Peptides and Proteins ,Nuclear Proteins ,Apoptosis ,YAP-Signaling Proteins ,Protein Serine-Threonine Kinases ,Article ,DNA-Binding Proteins ,Adult Stem Cells ,In Situ Nick-End Labeling ,Trans-Activators ,Animals ,Drosophila Proteins ,Drosophila ,lcsh:Q ,lcsh:Science ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,ComputingMilieux_MISCELLANEOUS ,Signal Transduction ,Transcription Factors - Abstract
To compensate for accumulating damages and cell death, adult homeostasis (e.g., body fluids and secretion) requires organ regeneration, operated by long-lived stem cells. How stem cells can survive throughout the animal life remains poorly understood. Here we show that the transcription factor Shavenbaby (Svb, OvoL in vertebrates) is expressed in renal/nephric stem cells (RNSCs) of Drosophila and required for their maintenance during adulthood. As recently shown in embryos, Svb function in adult RNSCs further needs a post-translational processing mediated by the Polished rice (Pri) smORF peptides and impairing Svb function leads to RNSC apoptosis. We show that Svb interacts both genetically and physically with Yorkie (YAP/TAZ in vertebrates), a nuclear effector of the Hippo pathway, to activate the expression of the inhibitor of apoptosis DIAP1. These data therefore identify Svb as a nuclear effector in the Hippo pathway, critical for the survival of adult somatic stem cells., Organ regeneration by stem cells is required to compensate for tissue damage during aging, although how stem cells are maintained in adulthood is poorly understood. Here, the authors show in Drosophila that Shavenbaby interacts with Yorkie, a mediator of Hippo signalling, to ensure adult stem cell survival.
- Published
- 2018