1. Sub-antimicrobial doses of doxycycline decreased bone loss related to ligature-induced periodontitis in hypertensive rats
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Mário Taba, Rachel F. Gerlach, Gustavo Henrique Apolinário Vieira, Sérgio Luis Scombatti de Souza, Patrícia Garani Fernandes, Michel Reis Messora, Daniela Bazan Palioto, Cristina Antoniali Silva, Flávia A.C. Furlaneto, Arthur B. Novaes, Janine M.T. Moura, Universidade de São Paulo (USP), and Universidade Estadual Paulista (Unesp)
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Male ,0301 basic medicine ,Molar ,medicine.medical_specialty ,medicine.medical_treatment ,Alveolar Bone Loss ,Inflammation ,Matrix metalloproteinase ,Gastroenterology ,Bone and Bones ,03 medical and health sciences ,0302 clinical medicine ,Rats, Inbred SHR ,Internal medicine ,medicine ,Animals ,Periodontitis ,Ligature ,Ligation ,General Dentistry ,Host modulation therapy ,Doxycycline ,biology ,business.industry ,Acid phosphatase ,030206 dentistry ,Cell Biology ,General Medicine ,medicine.disease ,Rats ,030104 developmental biology ,Blood pressure ,Otorhinolaryngology ,biology.protein ,Cardiovascular disease(s) ,Gene expression ,medicine.symptom ,business ,medicine.drug - Abstract
Made available in DSpace on 2019-10-06T16:21:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-05-01 Background/Objective: The beneficial effects of sub-antimicrobial dose doxycycline (SDD) associated with nonsurgical periodontal therapy are well documented. Recently, the effects of SDD on metalloproteinases have been investigated in the treatment of hypertension. The aim of this study was to evaluate the effects of SDD on ligature-induced periodontitis in spontaneously hypertensive rats (SHR). Methods: Fifty-four adult male rats were divided into three groups: SHR-C, SHR-L and SHR-L-DOX (C - Control; L – Ligature). In group SHR-L-DOX, animals were treated with daily 5 mg/kg SDD administration. In L groups, a ligature remained around mandibular first molars for the first 10 days. Each group was divided for euthanasia at 10 or 21 days. Microtomographic and histometric analyses were performed. Osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP) assay and gene expression of 84 inflammatory mediators by polymerase chain reaction (PCR) array. Results: Group SHR-L-DOX presented reduced systolic blood pressure when compared with group SHR-L at both 10 and 21 days (p < 0.05). Group SHR-L-DOX showed decreased bone and attachment loss in comparison with group SHR-L at both 10 and 21 days (p < 0.05). SDD treatment reduced the amount of TRAP-positive cells at 10 days (p < 0.05). Group SHR-L-DOX showed a downregulated inflammatory genes profile in comparison with SHR-L at 10 and 21 days. Conclusion: SDD therapy exerted systemic modulatory effect on inflammation with reduced periodontal tissue destruction in hypertensive rats. Department of Oral Surgery and Periodontology School of Dentistry of Ribeirao Preto University of Sao Paulo Department of Morphology Stomatology and Physiology School of Dentistry of Ribeirao Preto University of Sao Paulo Dental School of Araçatuba Univ Estadual Paulista – UNESP Department of Basic Sciences Dental School of Araçatuba Univ Estadual Paulista – UNESP Department of Basic Sciences
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- 2019
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