1. A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles
- Author
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Jiang, Linglei, Luirink, Joen, Kooijmans, Sander A A, van Kessel, Kok P M, Jong, Wouter, van Essen, Max, Seinen, Cor W, de Maat, Steven, de Jong, Olivier G, Gitz-François, Jerney F F, Hennink, Wim E, Vader, Pieter, Schiffelers, Raymond M, Pharmaceutics, Afd Pharmaceutics, Molecular Microbiology, AIMMS, LaserLaB - Molecular Biophysics, Pharmaceutics, and Afd Pharmaceutics
- Subjects
Surface Properties ,Blotting, Western ,Biophysics ,Cell Culture Techniques ,Outer membrane vesicles ,02 engineering and technology ,Extracellular vesicles ,Biochemistry ,Post insertion ,03 medical and health sciences ,Mice ,Microscopy, Electron, Transmission ,Labelling ,Cell Line, Tumor ,Animals ,Humans ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,Liposome ,Bacteria ,Chemistry ,Translation (biology) ,021001 nanoscience & nanotechnology ,Flow Cytometry ,Poly(ethylene glycol)lipids ,Immunohistochemistry ,Cell biology ,Bacterial Outer Membrane ,HEK293 Cells ,Post-insertion ,Nucleic acid ,Surface modification ,Electrophoresis, Polyacrylamide Gel ,0210 nano-technology ,Drug carrier - Abstract
Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a ‘post-insertion’ approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.
- Published
- 2021