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This study explored the colour–touch cross-modal correspondence and its impact on colour–touch multisensory perception. Two laboratory experiments were conducted based on a pre-experiment. In the first experiment, participants chose the colour according to their tactile sense against the vibration generated by the smartphone simulator. A positive cross-modal correspondence was obtained between the vibration amplitude and the colour chroma, indicating the existence of a colour–touch correspondence. The correspondence between vibration parameters and colour hue was preliminarily explored. The impact of the correspondence on the multisensory senses was investigated in the second experiment. Different stimulus combinations were played on a smartphone. The participants were asked to choose the perceived stimulus level. The data illustrated that a better correspondence improved judgement accuracy and reduced reactiontime. Overall, this study offers evidence for colour–touch cross-modal correspondence and presents its potential influence on the single-modal judgement in multimodal perception. The results can be applied in further cross-modal studies, assisting barrier-free society construction and multimodal interface design.

Alcohol consumption is regarded as one of the leading risk factors for secondary osteopenia. Angiogenesis and osteogenesis coupled by type-H vessels coordinate the biological process of bone homeostasis to prevent osteopenia. This study aimed to determine whether chronic alcohol inhibits type-H vessel-dependent bone formation. Two-month-old mice were fed with 5% (v/v) alcohol liquid diet (28% of calories) or normal liquid diet every day for 2 months. The tibias were isolated and detected with X-ray and microcomputed tomography. Paraffin-embedded or frozen tibial sections were prepared and used for immunohistochemical or immunofluorescence staining, respectively. Human umbilical vein endothelial cells (HUVECs) were treated with different concentrations of alcohol, including 0 mM (0%), 8.7 mM (0.5%), 52 mM (3%), or 87 mM (5%) alcohol for 12 h. The conditioned medium of the above HUVEC cells was collected to culture human bone marrow-mesenchymal stem cells (BM-MSCs), which were induced to differentiate into osteoblasts in vitro. The alcoholic diet retarded the bone growth and led to osteoporosis, impaired bone formation of osteoblasts, and decreased CD31

Many organisms can sense and respond to magnetic fields (MFs), with migratory species in particular utilizing geomagnetic field information for long-distance migration. Cryptochrome proteins (Crys) along with a highly conserved Iron-sulfur cluster assembly protein (i.e., MagR) have garnered significant attention for their involvement in magnetoresponse (including magnetoreception). However, in vivo investigations of potential transcriptional crosstalk between Crys and MagR genes have been limited. The brown planthopper, Nilaparvata lugens, is a major migratory pest insect and an emerging model for studying MF intensity-related magnetoresponse. Here, we explored in vivo transcriptional crosstalk between Crys (Cry1 and Cry2) and MagR in N. lugens. The expression of Crys and MagR were found to be sensitive to MF intensity changes as small as several micro-teslas. Knocking down MagR expression led to a significant downregulation of Cry1, but not Cry2. The knockdown of either Cry1 or Cry2 individually did not significantly affect MagR expression. However, their double knockdown resulted in significant upregulation of MagR. Our findings clearly indicate transcriptional crosstalk between MagR and Crys known to be involved in magnetoresponse. This work advances the understanding of magnetoresponse signaling and represents a key initial step towards elucidating the functional consequences of these novel in vivo interactions.

Pest control effectiveness and residues of pesticides are contradictory concerns in agriculture and environmental conservation. On the premise of not affecting the insecticidal effect, the pesticide residues in the later stage should be degraded as fast as possible. In the present study, composite nanoparticles in a double-layer structure, consisting of imidacloprid (IMI) in the outer layer and plant hormone 24-epibrassinolide (24-EBL) in the inner layer, were prepared by the W/O/W solvent evaporation method using Eudragit RL/RS and polyhydroxyalkanoate as wall materials. The release of IMI in the outer layer was faster and reached the maximum within 24 h, while the release of 24-EBL in the inner layer was slower and reached the maximum within 96 h. The contact angle of the composite nanoparticles was half that of the 5% IMI emulsifiable concentrate (EC), and the deposition of composite nanoparticles on rice was twice that of 5% IMI EC, which increased the pesticide utilization efficiency. Compared with the common pesticide, 5% IMI EC, the insecticidal effect of the composite nanoparticles was stronger than that of planthoppers, with a much lower final residue amount on rice after 21 days. The composite nanoparticles prepared in this study to achieve sustained release of pesticides and, meanwhile, accelerate the degradation of pesticide residues have a strong application potential in agriculture for controlling pests and promoting crop growth.

In order to remediate the phytotoxicity of quinclorac to tomato by multi-walled carbon nanotubes (MWCNTs), the adsorption of quinclorac to MWCNTs was monitored and the effect of MWCNTs on the phytotoxicity of quinclorac to tomato in soil were studied. The results showed that the Linear equation and Freundlich equation can well fit the adsorption isotherm of quinclorac in the soil containing MWCNTs. The adsorption of quinclorac in soil was significantly enhanced by the addition of MWCNTs; the K

Adsorption of test substances on micromembrane filters during sample pretreatment before qualitative and quantitative analysis has greatly affected the accuracy of the measurement. In the present study, it was found that the adsorption rate of pyraclostrobin reached 77.7-100% when water samples of pyraclostrobin (1 mL) were filtered with polyethersulfone (PES) and Nylon 6 filters. Therefore, the adsorption mechanisms were investigated from the kinetics, isotherms, and thermodynamics of the pyraclostrobin adsorption process, combined with attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) analysis. The results showed that PES accorded with second-order adsorption kinetics and Nylon 6 with first-order adsorption kinetics, and the correlation coefficient R

Central to homologous recombination (HR) is the assembly of Rad51 recombinase on single-strand DNA (ssDNA), forming the Rad51-ssDNA filament. How the Rad51 filament is efficiently established and sustained remains partially understood. Here, we find that the yeast ubiquitin ligase Bre1 and its human homolog RNF20, a tumor suppressor, function as recombination mediators, promoting Rad51 filament formation and subsequent reactions via multiple mechanisms independent of their ligase activities. We show that Bre1/RNF20 interacts with Rad51, directs Rad51 to ssDNA, and facilitates Rad51-ssDNA filament assembly and strand exchange in vitro. In parallel, Bre1/RNF20 interacts with the Srs2 or FBH1 helicase to counteract their disrupting effect on the Rad51 filament. We demonstrate that the above functions of Bre1/RNF20 contribute to HR repair in cells in a manner additive to the mediator protein Rad52 in yeast or BRCA2 in human. Thus, Bre1/RNF20 provides an additional layer of mechanism to directly control Rad51 filament dynamics.

Temperature variation and gas generation at different depths and positions in the coal combustion process were studied to determine the propagation and evolution of high temperature regions in the process of coal spontaneous combustion. This study selected coal samples from Mengcun, Shaanxi Province, People’s Republic of China, and developed a semi-enclosed experimental system (furnace) for simulating coal combustion. The thermal mass loss of coal samples under various heating rates (5, 10, and 15 °C/min) was analyzed through thermogravimetric analysis, and the dynamic characteristics of the coal samples were analyzed; the reliability of the semi-enclosed experimental system was verified through the equal proportional method of fuzzy response. The results revealed that the high-temperature zone was distributed nonlinearly from the middle to the front end of the furnace, and the temperatures of points in this zone decreased gradually as the layer depth increased. The apparent activation energy of the coal samples during combustion first increased and then decreased as the conversion degree increased. Furthermore, the proportion of mass loss and the mass loss rate in the coal samples observed in the thermogravimetric experiment was consistent with that observed in the first and second stages of the experiment conducted using the semi-enclosed system. The research findings can provide a theoretical basis for the prevention and control of high-temperature zones in coal combustion.

The human tumor suppressor Ring finger protein 20 (RNF20)-mediated histone H2B monoubiquitination (H2Bub) is essential for proper chromosome segregation and DNA repair. However, what is the precise function and mechanism of RNF20–H2Bub in chromosome segregation and how this pathway is activated to preserve genome stability remain unknown. Here, we show that the single-strand DNA-binding factor Replication protein A (RPA) interacts with RNF20 mainly in the S and G2/M phases and recruits RNF20 to mitotic centromeres in a centromeric R-loop-dependent manner. In parallel, RPA recruits RNF20 to chromosomal breaks upon DNA damage. Disruption of the RPA–RNF20 interaction or depletion of RNF20 increases mitotic lagging chromosomes and chromosome bridges and impairs BRCA1 and RAD51 loading and homologous recombination repair, leading to elevated chromosome breaks, genome instability, and sensitivities to DNA-damaging agents. Mechanistically, the RPA–RNF20 pathway promotes local H2Bub, H3K4 dimethylation, and subsequent SNF2H recruitment, ensuring proper Aurora B kinase activation at centromeres and efficient loading of repair proteins at DNA breaks. Thus, the RPA–RNF20–SNF2H cascade plays a broad role in preserving genome stability by coupling H2Bub to chromosome segregation and DNA repair.

Intelligent composite structures with self-aware functions are preferable for future aircrafts. The real-time location of damaged areas of composites is a key step. In this study, deep transfer learning was used to achieve the real-time location of damaged areas. The sensor network obtained acoustic emission signals from different damaged areas of the aluminum alloy plate. The acoustic emission time-domain signal is transformed into the input image by continuous wavelet transform. The convolutional neural network-based model automatically localized the damaged area by extracting features from the input image. A small amount of composite acoustic emission data was used to fine-tune some network parameters of the basic model through transfer learning. This enabled the model to classify the damaged area of composites. The accuracy of the transfer learning model trained with 900 samples is 96.38%, which is comparable to the accuracy of the model trained directly with 1800 samples; the training time of the former is only 17.68% of that of the latter. The proposed method can be easily adapted to new composite structures using transfer learning and a small dataset, providing a new idea for structural health monitoring.

Irritable bowel syndrome (IBS) is a common digestive disorder observed in clinics. Current studies suggest that the pathogenesis of the disease is closely related to abnormal brain–gut interactions, hypokinesia, visceral sensory hypersensitivity in the gastrointestinal tract, and alterations in the intestinal microenvironment. However, it is difficult for a single factor to explain the heterogeneity of symptoms. The Rome IV criteria emphasized the holistic biologic-psycho-social model of IBS, suggesting that symptoms of the disease are closely related to neurogastroenterology and various abnormalities in brain–gut interaction. This study comprehensively reviewed the relationship between the brain–gut axis and IBS, the structure of the brain–gut axis, and the relationship between the brain–gut axis and intestinal microenvironment, and discussed the relationship between the abnormal regulation of the nervous system, endocrine system, and immune system and the incidence of IBS on the basis of brain–gut axis. In terms of treatment, acupuncture therapy can regulate the neuroendocrine-immune system of the body and improve the intestinal microenvironment, and it has the advantages of safety, economy, and effectiveness. We study the pathogenesis of IBS from local to global and micro to macro, and review the use of acupuncture to treat the disease as a whole so as to provide new ideas for the treatment of the disease.

The existing displacement of parallel + serial type hybrid mechanisms is mainly solved by the equivalent serial mechanism (SM) method. However, a large number of lower mobility parallel mechanisms (PMs) that have high coupled motions at the end-effector cannot be equivalent to SMs. Thus, the displacement problem especially for the inverse displacement of this type of hybrid mechanisms has not been well solved. On the basis of this situation, this article takes a 6-degrees-of-freedom (DOFs) 3-UPU + 3R hybrid mechanism as an example to give a general method to solve the displacement problem. First, based on the inverse displacement and pose coupling relationship of the 3-UPU PM, its forward displacement is solved by Sylvester’s dialytic elimination method, and then the forward displacement of the 3-UPU + 3R hybrid mechanism is obtained by the superposition method. Second, by skillfully dealing with the relationship between coupling motions of the 3-UPU PM and the motion of hybrid mechanism, three nonlinear equations containing three unknown motion parameters are obtained, and the inverse displacement problem is solved using Sylvester’s dialytic elimination. The research in this article is valuable in the kinematics modeling of hybrid mechanisms.

Background Heme has attracted much attention because of its wide applications in medicine and food. The products of genes hemBCDEFY convert 5-aminolevulinic acid to protoporphyrin IX (PPIX; the immediate precursor of heme); protoporphyrin ferrochelatase (FECH) inserts Fe2+ into PPIX to generate heme. Biosynthesis of heme is limited by the need for optimized expression levels of multiple genes, complex regulatory mechanisms, and low enzymatic activity; these problems need to be overcome in metabolic engineering to improve heme synthesis. Results We report a heme biosensor-guided screening strategy using the heme-responsive protein HrtR to regulate tcR expression in Escherichia coli, providing a quantifiable link between the intracellular heme concentration and cell survival in selective conditions (i.e., the presence of tetracycline). This system was used for rapid enrichment screening of heme-producing strains from a library with random ribosome binding site (RBS) variants and from a FECH mutant library. Through up to four rounds of iterative evolution, strains with optimal RBS intensities for the combination of hemBCDEFY were screened; we obtained a PPIX titer of 160.8 mg/L, the highest yield yet reported in shaken-flask fermentation. A high-activity FECH variant was obtained from the saturation mutagenesis library. Fed-batch fermentation of strain SH20C, harboring the optimized hemBCDEFY and the FECH mutant, produced 127.6 mg/L of heme. Conclusion We sequentially improved the multigene biosynthesis pathway of PPIX and performed in vivo directed evolution of FECH, based on a heme biosensor, which demonstrated the effectiveness of the heme biosensor-based pathway optimization strategy and broadens our understanding of the mechanism of heme synthesis.

In this work, the explosion thermal behavior of H2/CH4/air mixtures, at different equivalence ratios (0.6–1.6) and hydrogen volume fractions (0%–100), was investigated in a confined 20-L chamber. The parameters of explosion time and pressure, as well as the explosion heat loss were quantitatively studied and analyzed. Moreover, the dominant chain reactions of the explosion process and heat release were identified via the detailed mechanism of the Foundational Fuel Chemistry Model (FFCM1). The results indicated that an increased H2 volume fraction in the mixtures increased the peak explosion pressure, maximum pressure rise rate and deflagration index. In addition, the explosion duration and fast-burning period were greatly shortened. Both the adiabatic flame temperature and thermal diffusivity monotonically increased with increasing H2 volume ratio. Moreover, the enhancement effect of the H2 ratio on the thermal diffusivity of H2/CH4 mixtures was more prominent for fuel-rich mixtures than for fuel-lean mixtures. The obtained quantitative results are helpful for developing measures to prevent the potential explosion accidents.

Coal-bearing fractured aquifer is regarded as one of the most dynamic mine water inrush sources, and after pumping and treating, it can be used as a water supply for coal mine production, coal preparation plant, rural irrigation, and even reserved drinking water source. Hence, this study focuses on the hydrochemical characteristics, ion source, and water quality evaluation with respect to drinking and irrigation of the coal-bearing fractured aquifer in Dingji coal mine, Huainan coalfield, China. Descriptive statistics and hydrochemical classification diagrams including the Piper diagram and Chadha rectangular diagram were carried out to depict the hydrochemical characteristics and facies. The water quality of the aquifer was assessed for irrigation and drinking purposes using the WHO threshold value, water quality index (WQI), SAR, % Na and RSC. Hydrochemical formation mechanism and solute origin of major ions were explained by Gibbs diagram, bivariate diagrams, and multivariate statistical analysis. The results show that the dominant hydrochemical facies are the Cl-Na type and the HCO3-Na type. The sequence of ions is Na+ > Ca2+ > Mg2+ for cations, and HCO3? > Cl- > SO42- > CO32- for anions. The main solute sources are controlled by various factors including the dissolution of halite, sulfate, and carbonate rocks, the weathering of silicate, and cation exchange. Water quality assessment based on WQI suggests that none of the samples fall under the excellent category, even 32.5% is not suitable for direct drinking. Meanwhile, the samples of the aquifer are generally unsuitable for irrigation. Before utilization for irrigation and even drinking, appropriate water treatment should be applied to guarantee its security during usage.

Epithelial ovarian cancer (EOC) is a gynecological disease with the highest mortality. With the lack of understanding of its pathogenesis, no accurate early diagnosis and screening method has been established for EOC. Studies revealed the multi-faceted function of Wilms’ tumor (Wt1) genes in cancer, which may be related to the existence of multiple alternative splices. Our results show that Wt1 (+KTS) or Wt1 (−KTS) overexpression can significantly promote the proliferation and migration of human ovarian epithelial cells HOSEpiC, and Wt1 (+KTS) effects were more evident. To explore the Wt1 (+/−KTS) variant mechanism in HOSEpiC proliferation and migration and ovarian cancer (OC) occurrence and development, this study explored the differential regulation of Wt1 (+/−KTS) in HOSEpiC proliferation and migration by transcriptome sequencing. OC-related hub genes were screened by bioinformatics analysis to further explore the differential molecular mechanism of Wt1 (+/−KTS) in the occurrence of OC. Finally, we found that the regulation of Wt1 (+/−KTS) variants on the proliferation and migration of HOSEpiC may act through different genes and signaling pathways and screened out key genes and differentially regulated genes that regulate the malignant transformation of ovarian epithelial cells. The implementation of this study will provide new clues for the early diagnosis and precise treatment of OC.

Background: Acute kidney injury (AKI) induced by renal ischemia/ reperfusion (I/R) leads to a sharp decrease in renal function. Although it has been shown that miRNA-21 is dysregulated in patients who has AKI, it’s related regulation mechanism isn’t clear yet. This study aims to explore the potential mechanisms of miRNA-21-3p and miRNA-21-5p in I/R induced AKI to chronic kidney disease (CKD). Methods: The miRNA expression profile data (GSE125305) and the mRNA expression profile data (GSE148420) of rat model which include I/R induced AKI were downloaded from the NCBI⁃GEO database, and both datasets randomly divided the rats into two groups: the sham operation group and the ischemia reperfusion injury (IRI) group which received IR operation. Comprehensive analysis was used by bioinformatics method. Firstly, the miRNAs expression changes in each group at different time periods were studied. Then, the significantly different miRNAs in each group were unified, and the target genes of miRNAs were obtained. Through the protein-protein interaction (PPI) network, the mutual interaction relationships of target genes were obtained. The regulatory relationships between mRNA-mRNA and miRNA-mRNA were visualized. Next, to find the potential target genes of miRNA-21-3p and miRNA-21-5p, the significantly different mRNAs were analyzed between the group of control-3 days after surgery and the group of 3 days-7 days after surgery. Subsequently, we obtained transcription factors (TFs) that regulate pri-mir-21 and target genes, and further constructed the regulatory network of TFs-miRNA-mRNA. Finally, GO and KEGG enrichment analysis of target genes were carried out. Results: A total of 29 miRNAs with significant differences were obtained in this study. Based on the regulatory relationship between target genes and miRNAs and the changes in the expression of target genes, we believe that miRNA-21-3p tends to target to Fshb, and miRNA-21-5p tends to target to Tagln, Pdpn and Tpm1. Subsequently, we obtained the same TFs which regulate pri-mir-21 and target genes, and constructed the mutual regulatory relationship among miRNA-21-3p, miRNA-21-5p, transcription factors which include Hnf4a and Rela, as well as target genes which include Fshb, Tagln, Pdpn and Tpm1. Finally, the results of enrichment analysis of target genes include muscle organ development, positive regulation of cell adhesion, lymphangiogenesis, epithelial cell fate commitment, regulation of cell morphogenesis, peptide hormone processing, actin filament, actin binding, chemokine binding, chaperone binding, and dilated cardiomyopathy, and illustrated underlying molecular mechanisms. Conclusions: Through participating in functions and pathway of enrichment, target genes play an important role in the progression of IRI induced AKI to CKD. Meanwhile, These target genes possess a potential to be novel biomarkers and molecular targets for the diagnosis and treatment of renal diseases in the future.

A gold-catalyzed oxidative three-component reaction of terminal alkynes with alcohols and quinone monoimines has been disclosed, affording α-ketoacetals in good to excellent yields. By using quinone monoimines as electrophiles for the interception of the

Representative driving cycles and specific vehicle types play an important role in analyzing energy consumption to provide valuable references for manufacturers and government inspections. In this study, a parallel plug-in hybrid electric vehicle with different driven configurations is employed to analyze energy consumption under a typical driving cycle. Specifically, a typical driving cycle of Tianjin is constructed by integrating the clustering and Markov chain algorithms. Considering the various layouts of energy systems in plug-in hybrid electric vehicles, three different driven configurations (P2, P3, P4) of the parallel plug-in hybrid electric vehicle are established. Finally, the energy consumptions of the different configurations are analyzed under the proposed typical driving cycle and the compared China Automotive Test Cycle. Results of two driving cycles demonstrate that the fuel consumption of the P3 configuration is lower than that of the P2 and P4 configurations, respectively, while the purely electric driving range of the P3 configuration is longer than the other two configurations, respectively. These results provide a basis for the formulation of China’s new light vehicle emission standards and reduce exhaust emission by making the vehicles more economically suitable for the road conditions in Tianjin.

A deposited layer of Sn-based Babbitt alloys was fabricated on the surface of the steel substrate via the arc deposition technique. The effect of the composition of Zn on the microstructure and mechanical properties has been systematically studied. The morphology of Babbitt alloys and the interfacial microstructure of the interface between the cladding layer and substrate have been investigated. The experimental results revealed that SnSb phases and Cu6Sn5 phases become more uniformly dispersed in the Sn-matrix as Zn composition increased, which resulting in the improvement of hardness and tensile strength. The enhanced performance can be attributed to precipitation strengthening of the newly formed substitutional solid solution Cu6Zn0.5Sn4.5. The bonding strength of Babbitt and steel has been tested according to the standard destructive testing method of sliding bearing. The bonding strength increased and decreased with the increment of Zn, since excessive Zn led the fracture site to shift from the SnSb phase to the segregated Cu6Sn4.5Zn 0.5 at the interface near IMC layers.

Object detection in remote sensing images has important applications in various aspects. Object detection algorithms with deep convolutional neural networks (DCNNs) have made remarkable progress. However, when processing objects on vastly multiple scales in high-resolution optical remote sensing images, there is a high computational cost. Therefore, to simplify neural network multiscale training and inference, an automatic multiscale inference framework is proposed to balance the speed and accuracy of object detection. We use an attention mechanism that uses a key-point network to predict regions with small objects on a coarse scale and only process regions obtained from the first stage on finer scales instead of processing an entire larger scale image. The fully convolutional neural network (CNN) that is used in training and detecting is not affected by the image input resolution. The experiments are carried out using the NWPUVHR-10 data set, and the experimental results show that these methods can improve the training efficiency and detection accuracy in remote sensing images.

Systemic inflammatory and autoimmune manifestations (SIAMs) are frequently reported in Myelodysplastic syndromes (MDS). Studies focused on the impact of SIMAs on survival outcomes of MDS remains controversial. We performed this systematic review and meta-analysis to determine the association of SIAMs with overall survival, median survival, rate of acute myeloid leukemia transformation and mortality of MDS.An electronic search was conducted in 4 databases without any language restrictions, including PubMed, EMBASE, Medicine and Cochrane library up to April 30, 2021.The 18 studies included a total of 4603 MDS patients, of which 1175 (25.5%) patients had SIAMs. MDS patients with SIAMs had a statistically shorter overall survival compared with patient without SIAMs (Hazard ratio, 2.43; 95% confidence interval [CI], 1.34-4.41; P .01). Our results were most compatible with no effect of SIAMs on median survival, rate of acute myeloid leukemia transformation and mortality (Median survival ratio, 1.16; 95% CI, 0.91-1.47; Odds ratio, 0.96; 95% CI, 0.63-1.45 and 1.2; 95% CI, 0.84-1.7, respectively).In this systematic review and meta-analysis, SIAMs appeared to have an adverse effect on overall survival of MDS patients. This finding suggested that SIAMs may be a potential independent prognostic factor for MDS.

Glycolytic flux is a fundamental index in microbial cell factories. A glycolytic flux biosensor that can monitor glucose metabolism efficiency is a promising strategy in rewiring metabolic flux to balance growth and biosynthesis. A key design feature of the glycolytic flux biosensors is the interaction between the global transcriptional factor Cra and its regulated promoters. However, overexpression and mutation of Cra has unpredictable effects on global metabolism in Escherichia coli. Therefore, new orthogonal biosensor design strategies should be developed to circumvent metabolic issues. In this report, the promoters in glycolytic flux biosensor were replaced with synthetic promoters of varying strengths or phage-derived promoters, and the Cra DNA-binding sites were deployed into promoters at different positions and distances to yield biosensors. The de nova biosensors that depended on Cra could sense Fructose-1,6-diphosphate (FBP) with broad dynamic ranges and low basal leakage. Then the negative-response biosensors were applied to fine-tune the target ATP synthesis gene, leading to the desired increase in pyruvate production (the highest 9.66 g/L) and cell growth. Moreover, the membrane synthesis gene plsC was also dynamically activated by the positive-response biosensor, leading to effective accumulation of lycopene in the cell membrane and a 50-fold increase in lycopene titre (100.3 mg/L) when compared with the control strain, demonstrating the effective and broader usages of our biosensors.

Acute myeloid leukaemia (AML) is a heterogeneous disease associated with poor outcomes. To identify AML-specific genes with prognostic value, we analysed transcriptome and clinical information from The Cancer Genome Atlas (TCGA) database, Gene Expression Omnibus (GEO) datasets, and Genotype-Tissue Expression (GTEx) project. The metabolism-related gene, SLC25A21 was found to be significantly downregulated in AML, and was associated with high white blood cell (WBC) counts, high pretrial blood (PB) and bone marrow (BM) blast abundance, FLT3 mutation, NPM1 mutation, and death events (all p value SLC25A21 in our clinical cohort, and found that SLC25A21 was downregulated in AML. Moreover, we identified low expression of SLC25A21 as an independent prognostic factor by univariate Cox regression (hazard ratio [HR]: 0.550; 95% Confidence interval [CI]: 0.358–0.845; p value = 0.006) and multivariate Cox regression analysis (HR: 0.341; 95% CI: 0.209–0.557; p value SLC25A21 expression level, patients in the TCGA-LAML cohort were divided into low- and high-expression groups. Gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DEGs highlighted growth factor binding, extracellular structure organization, cytokine‒cytokine receptor interaction, etc. The results of gene set enrichment analysis (GSEA) indicated that the epithelial-mesenchymal transition, KRAS signalling, oxidative phosphorylation, and reactive oxygen species pathways were enriched. Through gene coexpression and protein‒protein interaction (PPI) network analysis, we identified two hub genes, EGFR and COL1A2, which were linked to worse clinical outcomes. Furthermore, we found that lower SLC25A21 expression was closely associated with a significant reduction in the levels of infiltrating immune cells, which might be associated with immune escape of AML cells. A similar trend was observed for the expression of checkpoint genes (CTLA4, LAG3, TIGIT, and HAVCR2). Finally, drug sensitivity testing suggested that the low-expression SLC25A21 group is sensitive to doxorubicin, mitomycin C, linifanib but resistant to JQ1, belinostat, and dasatinib. Hence, our study demonstrated that a low expression level of SLC25A21 predicts an unfavourable prognosis in patients with AML.

The cross temperature measuring device is applied to monitor the top temperature and gas flow distribution in the blast furnace (BF) during the ironmaking process. However, the temperature of the B...

Lan-gan region, one of traditional agricultural production base in northern Anhui Province, East China, has rare hydrochemical reports on the formation mechanism and irrigation assessment of the Quaternary deeper confined groundwater aquifer. To better master hydrochemical characteristics, solute source, and irrigation properties, twenty-five water samples were collected from individual wells. The Gibbs diagram, Piper diagram, ionic ratio, and multivariate statistical analysis were carried out to delineate the hydrochemical facies and water–rock interactions. Meanwhile, USSL and Wilcox diagrams, and irrigation water quality indices were conducted to illustrate the suitability for irrigation. The results indicate that cations and anions are in the order of Ca2+ > Na+ > Mg2+ >K+ and HCO3− > SO42− > Cl−, respectively. Ca-HCO3 is the main hydrochemical type. Water–rock interactions include silicate weathering, carbonate dissolution, halite dissolution and sulfate dissolution. Based on electrical conductivity (EC) value, the majority of the samples are permissible for irrigation, whereas only 12% of the samples were found good for irrigation. The risidual sodium carbonate (RSC), sodium adsorption ratio (SAR), and percentage sodium (%Na), combined with Wilcox and USSL diagrams, demonstrate that the groundwater samples are permissible for irrigation purposes.

Background Closed reduction and pinning entry fixation have been proposed as treatment strategies for displaced supracondylar humeral fractures (SCHFs) in children. However, controversy exists regarding the selection of the appropriate procedure. Hence, this meta-analysis was conducted to compare the effect of lateral and crossed pin fixation for pediatric SCHFs, providing a reference for clinical treatment. Methods Online databases were systematically searched for randomized controlled trials (RCTs) comparing lateral pinning entry and crossed pinning entry for children with SCHFs. The primary endpoints were iatrogenic ulnar nerve injuries, complications, and radiographic and functional outcomes. Results Our results showed that iatrogenic ulnar nerve injuries occurred more commonly in the crossed pinning entry group than in the lateral pinning entry group (RR = 4.41, 95% CI 1.97–9.86, P < 0.05). However, its risk between the crossed pinning with mini-open incisions group and the lateral pinning entry group was not significantly different (RR = 1.58, 95% CI 0.008–29.57, P = 0.76). The loss of reduction risk was higher in the lateral pinning entry group than in the crossed pinning entry group (RR = 0.66; 95% CI 0.49–0.89, P < 0.05). There were no significant differences in the carry angle, Baumann angle, Flynn scores, infections, and other complications between these two groups. Conclusions The crossed pinning entry with mini-open incision technique reduced the loss of reduction risk, and the risk of iatrogenic ulnar nerve injury was lower than in the lateral pinning entry group. The crossed pinning entry with mini-open incision technique is an effective therapeutic strategy for managing displaced supracondylar humeral fractures in children.