1. Identification and Characterization of 33 Bacillus cereus sensu lato Isolates from Agricultural Fields from Eleven Widely Distributed Countries by Whole Genome Sequencing
- Author
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Aggerbeck, Marie Rønne, Zghal, Raida Zribi, Hendriksen, Niels Bohse, Zervas, Athanasios, Aggerbeck, Marie, Allaga, Henrietta, Güzel, Mustafa, Hendriks, Marc, Jonuškienė, IIona, Kedves, Orsolya, Kupeli, Ayse, Lamovšek, Janja, Mülner, Pascal, Munday, Denise, Namli, Şahin, Samut, Hilal, Tomičić, Ružica, Tomičić, Zorica, Yeni, Filiz, Zghal, Raida, Zhao, Xingchen, Sanchis-Borja, Vincent, Hendriksen, Niels, Aarhus University [Aarhus], Université de Sfax - University of Sfax, University of Szeged [Szeged], Hitit University, Wageningen University and Research [Wageningen] (WUR), Kaunas University of Technology (KTU), Middle East Technical University [Ankara] (METU), Agricultural Institute of Slovenia, Agricultural Institute, Graz University of Technology [Graz] (TU Graz), Sumitomo Chemical Agro Europe, Partenaires INRAE, University of Novi Sad, Centre de Biotechnologie de Sfax (CBS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), European Cooperation in Science and Technology's COST-action 16110, [Belirlenecek], and MDPI AG (Basel, Switzerland)
- Subjects
Microbiology (medical) ,Ecotypes ,Technology and Engineering ,[SDV]Life Sciences [q-bio] ,Bacillus toyonensis ,DIVERSITY ,ecotypes ,Bacillus thuringiensis ,AFFILIATION ,Bacillus mosaicus ,Bacillus ,Context (language use) ,mycoides ,GROUP STRAINS ,phylogeny ,Microbiology ,Article ,03 medical and health sciences ,Biointeractions and Plant Health ,Sensu ,geographical-distribution ,Phylogenetics ,Virology ,parasitic diseases ,lcsh:QH301-705.5 ,Phylogeny ,030304 developmental biology ,Bacillus mycoides ,Genetics ,Whole genome sequencing ,patho-types ,0303 health sciences ,biology ,Phylogenetic tree ,Ecotype ,030306 microbiology ,fungi ,Geographical-distribution ,biology.organism_classification ,bacterial infections and mycoses ,Cereus ,lcsh:Biology (General) ,WEIHENSTEPHANENSIS ,Identification (biology) ,Patho-types ,geographic locations - Abstract
The phylogeny, identification, and characterization of 33 B. cereus sensu lato isolates originating from 17 agricultural soils from 11 countries were analyzed on the basis of whole genome sequencing. Phylogenetic analyses revealed all isolates are divided into six groups, which follows the generally accepted phylogenetic division of B. cereus sensu lato isolates. Four different identification methods resulted in a variation in the identity of the isolates, as none of the isolates were identified as the same species by all four methods&mdash, only the recent identification method proposed directly reflected the phylogeny of the isolates. This points to the importance of describing the basis and method used for the identification. The presence and percent identity of the protein product of 19 genes potentially involved in pathogenicity divided the 33 isolates into groups corresponding to phylogenetic division of the isolates. This suggests that different pathotypes exist and that it is possible to differentiate between them by comparing the percent identity of proteins potentially involved in pathogenicity. This also reveals that a basic link between phylogeny and pathogenicity is likely to exist. The geographical distribution of the isolates is not random: they are distributed in relation to their division into the six phylogenetic groups, which again relates to different ecotypes with different temperature growth ranges. This means that we find it easier to analyze and understand the results obtained from the 33 B. cereus sensu lato isolates in a phylogenetic, patho-type and ecotype-oriented context, than in a context based on uncertain identification at the species level.
- Published
- 2020