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(Neuron 110, 1671–1688; May 18, 2022) It was brought to our attention that there is a clerical mistake in one of the panels of Figure 1 (Figure 1E) in our recent publication. The representative blots for the CNS Stmn2 protein levels in the F 2 generation of Stmn2 mutants included an incorrect duplicated blot for the GAPDH loading control in the brain samples. We have corrected this error and replaced the figure online with one that includes the correct blot for the GAPDH loading control for those samples. We would like to profoundly apologize to the readers for the oversight and honest mistake.[Formula

Rationally designed siRNA delivery materials that are enabled by lipid-modified aminoglycosides are demonstrated. Leading materials identified are able to self-assemble with siRNA into well-defined nanoparticles and induce efficient gene knockdown both in vitro and in vivo. Histology studies and liver function tests reveal that no apparent toxicity is caused by these nanoparticles at doses over two orders of magnitude., Damon Runyon Cancer Research Foundation (DFS-#2050-10), Alnylam Pharmaceuticals (Firm), National Institutes of Health (U.S.) (grant EB000244), National Institutes of Health (U.S.) (grant 1-RO1-CA132091-03)

The development of responsive nanomaterials, nanoscale systems that actively respond to stimuli, is one general goal of nanotechnology. Here we develop nanoparticles that can be controllably triggered to synthesize proteins. The nanoparticles consist of lipid vesicles filled with the cellular machinery responsible for transcription and translation, including amino acids, ribosomes, and DNA caged with a photolabile protecting group. These particles served as nanofactories capable of producing proteins including green fluorescent protein (GFP) and enzymatically active luciferase. In vitro and in vivo, protein synthesis was spatially and temporally controllable, and could be initiated by irradiating micrometer-scale regions on the time scale of milliseconds. The ability to control protein synthesis inside nanomaterials may enable new strategies to facilitate the study of orthogonal proteins in a confined environment and for remotely activated drug delivery., National Cancer Institute (U.S.) (MIT-Harvard Center for Cancer Nanotechnology Excellence Grant U54 CA151884), Marie D. and Pierre Casimir-Lambert Fund, National Cancer Institute (U.S.) (Cancer Center Support (Core) Grant P30-CA14051), National Institutes of Health (U.S.) (Grant EB000244)