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Turcicum leaf blight (TLB) is a common foliar disease of maize in Mexico that is caused by the fungal pathogen Exserohilum turcicum. The most effective management strategy against TLB is monogenic race-specific resistance. Among the 140 E. turcicum isolates from symptomatic leaves collected from maize fields in Mexico, 100 were obtained from tropical (Veracruz) and temperate areas (Estado de México) between 2010 and 2019, and 40 isolates were obtained from tropical (Sinaloa, Tamaulipas, Veracruz, and Chiapas), subtropical (Nayarit, Jalisco, and Guanajuato), and temperate areas (Estado de Mexico, Hidalgo, and Puebla) collected in 2019. All the isolates caused TLB symptoms on the positive control (ht4), showing that they were all pathogenic. Six physiological races of E. turcicum (2, 3, 23, 3N, 23N, and 123N) were identified based on resistant or susceptible responses displayed by five maize differential genotypes (A619Ht1, A619Ht2, A619Ht3, B68HtN, and A619ht4). The most common was race 23, accounting for 68% of the isolates, followed by races 23N, 123N, 3, 2, and 3N at 15, 8, 6, 2, and 1%, respectively. Race 123N was able to infect the greatest number of maize differential genotypes used in the study. Race 123N was detected in Sinaloa and Estado de México. Race 3 was detected in Nayarit and Jalisco. Race 2 was detected in Jalisco, Estado de México, and Veracruz, and race 3N was detected in Tamaulipas. Race 23 was equally dominant in the tropical, subtropical, and temperate regions, while race 123N was more common in the tropical environment, and race 23N was more common in the tropical and temperate environments. There was no evidence for shifts in the races between 2010 and 2019.

La emergencia sanitaria por la propagación del virus covid-19, ha cambiado la vida de la población mundial, el confinamiento se ha hecho obligatorio para reducir los riesgos de contagio, lo que ha generado reducción de las actividades de participación masiva incluidas las actividades del sistema educativo en todas sus modalidades y niveles, estando en facultad de circular solo para provisionarse de alimentos y demás insumos vitales para la población, en este contexto y con la decisión del Estado de reactivar los estudios de forma virtual, se hace necesario realizar estudios que evidencien cual es la posibilidad de la población de participar de la modalidad virtual para sus estudios manteniendo el principio de inclusión. El presente estudio radica en analizar el acceso al internet y equipos tecnológicos para su permanencia en el sistema educativo de los estudiantes de la provincia de Los Ríos, para lo cual, hace uso de métodos cualitativos y cuantitativos de nivel teórico: análisis-síntesis, inducción-deducción e históricológico, los mismos que permiten el procesamiento de la información proveniente de experiencia de profesional directivo, docente, estudiante universitario, bachiller y de los resultados obtenidos de la encuesta a estudiantes de la provincia en una muestra de 330 participantes, los cuales evidencian un porcentaje que no tiene acceso al internet ni a equipos tecnológicos para ajustarse a la modalidad virtual, por lo cual se generan alternativas que permitan mantenerlos incluidos y garanticen su permanencia sin ser perjudicados con la pérdida de sus niveles de estudio.

As part of an ongoing treatment of species of the Brevantherum clade of Solanum, a new species from the Atlantic Forest of Brazil was discovered and is described here. Solanum helix Giacomin & Stehmann sp. nov. is a species well represented in herbaria that has been overlooked for almost two centuries. It has a unique expanded non-inflated fruiting calyx that resembles a propeller and that is not found in any unarmed species of Solanum. This discovery highlights the importance of continued herbarium and field work in hyperdiverse ecosystems, such as the Atlantic Forest in South America. Taxonomic affinities are discussed and images, as well as mapped distribution, are given. A key to closely related species for NE Brazil is also provided.

La educación rural se caracteriza por tener instituciones educativas que brindan principalmente la educación básica y en los últimos años se ha universalizado la educación inicial, teniendo en las cabeceras rurales el bachillerato, niveles que presentan limitaciones y que han sido superados de cierta forma con la llegada de la tecnología en los sectores donde existe acceso al internet, sin embargo al referirse a la educación superior la situación es diferente, por cuanto, dichas instituciones se encuentran sobre todo en las capitales de provincia y sin programas efectivos de inclusión, que no es percibida por la inexistencia de estudios que demuestren la presencia de bachilleres de escasos recursos económicos que tienen oportunidad de continuar sus estudios y obtener una profesión. Esta investigación pretende visibilizar a este grupo de jóvenes que a pesar de tener capacidad intelectual no pueden acceder a la educación superior por presentar limitaciones económicas y familiares. Con su enfoque cualitativo, basado en la descripción de los hallazgos, es descriptivo, apoyado en métodos analítico-sintético e inductivo-deductivo, que permiten el tratamiento de la información y el análisis del objeto de investigación, con técnicas como la observación y la encuesta, utilizando los medios tecnológicos, con un cuestionario de preguntas para la recolección de la información a una muestra de 376 bachilleres, concluyéndose que la mayoría de los bachilleres de las zonas rurales no continúan con sus estudios superiores debido a las limitaciones económicas que presentan y por factores asociados como los embarazos prematuros, las uniones matrimoniales anticipadas, responsabilidad económica en el hogar y fallecimiento de sus padres.

El objetivo de este trabajo fue evaluar el comportamiento postcosecha del fruto de mango cv Ataulfo en estado de madurez fisiológica como respuesta a la infección inducida por Colletotrichum siamense y Colletotrichum asianum. Se inocularon mangos con C. siamense y C. asianum y se almacenaron durante 10 días a 28 °C y HR de 85-90%. En los frutos se evaluó la virulencia de Colletotrichum spp. Y su efecto en las variables de calidad físicas, químicas y la respiración. A los 10 días de almacenamiento, C. siamense mostró mayor virulencia que C. asianum en los frutos de mango, con algunas diferencias en el color interno entre los frutos inoculados y los testigos. Los frutos inoculados con C. asianum presentaron mayor actividad respiratoria y menor textura con respecto al testigo. Las variables de calidad físicas y químicas en los frutos mostraron diferencias significativas con respecto al tiempo de almacenamiento, pero no por la presencia del hongo. La infección de los mangos causada por las dos especies de Colletotrichum se manifestó como manchas necróticas en la zona infectada de los frutos, afectando la apariencia y la calidad visual.

Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is a major disease of solanaceous crops worldwide. In May 2022, symptoms of wilting, yellowing, and reduced growth were observed on eggplant (Solanum melongena) cv. Barcelona in a commercial greenhouse located in Culiacán, Sinaloa, Mexico. The disease incidence was recorded up to 30%. Sections of stems from diseased plants showed discoloration of the vascular tissue and the pith. Colonies with typical RSSC morphology were isolated from five eggplant stems on Petri plates containing casamino acid-peptone-glucose (CPG) medium supplemented with 1% 2,3,5-triphenyltetrazolium chloride (TZC), and incubated at 25°C for 48-h (Schaad et al. 2001; Garcia et al. 2019). On CPG medium + TZC, white and irregular colonies with pinkish centers were observed. On King’s B medium, mucoid and white colonies were produced. The strains were Gram-negative in the KOH test and were nonfluorescent on King’s B medium. Strains were positive using commercial Rs ImmunoStrip® (Agdia, USA). For molecular identification, DNA was extracted, and the partial endoglucanase gene (egl) was amplified by PCR and sequenced using the primer pair Endo-F/Endo-R (Fegan and Prior 2005). BLASTn searches showed 100% identity with available sequences of R. pseudosolanacearum from Musa sp. in Colombia (MW016967) and from Eucalyptus pellita in Indonesia (MW748363, MW748376, MW748377, MW748379, MW748380, MW748382). To confirm the bacterial identity, DNA was amplified with the primers 759/760 (Opina et al. 1997) and Nmult21:1F/Nmult22:RR (Fegan and Prior 2005) to generate 280 and 144-bp amplicons for RSSC and phylotype I (= R. pseudosolanacearum), respectively. A phylogenetic analysis was performed using the Maximum Likelihood method and the strain was distinguished as R. pseudosolanacearum sequevar 14. The strain (CCLF369) is currently preserved in the Culture Collection of the Research Center for Food and Development (Culiacán, Sinaloa, Mexico) and the sequence was deposited in GenBank (accession number OQ559102). Pathogenicity tests were performed by injection of 20-μl of a bacterial suspension (108 CFU/ml) at the base of the stem of five eggplants cv. Barcelona. Five plants inoculated with sterile distilled water were used as control. Plants were kept in a greenhouse at 28/37°C (night/day) for 12 days. All inoculated plants exhibited wilting, chlorosis, and necrosis of leaves between 8 and 11 days after inoculation, whereas control plants remained asymptomatic. The bacterial strain was only isolated from symptomatic plants and confirmed to be R. pseudosolanacearum using the molecular techniques mentioned above, fulfilling Koch´s postulates. Ralstonia pseudosolanacearum has been previously reported to cause bacterial wilt of tomato in Sinaloa, Mexico (García-Estrada et al. 2023); however, to our knowledge, this is the first report of R. pseudosolanacearum infecting eggplant in Mexico. Further studies on epidemiology and management strategies for this disease are required on vegetable crops in Mexico.

Peronospora tabacina is considered the main limiting factor in tobacco production worldwide. In Mexico, information on the genetic diversity of this pathogen is scarce; therefore, the objective of this research was to evaluate 12 microsatellites in 20 isolates collected in the states of Nayarit, Chiapas, and Veracruz. PCR amplification and sequencing of these microsatellites were performed; as well as the alignment and comparison of the sequences deposited in the GenBank database. A total of 19 isolates showed amplification for the 12 microsatellites evaluated, while in one of the isolates, the amplification of two microsatellites was not observed, it being possible to determine that P. tabacina isolates present in Nayarit, Chiapas, and Veracruz are genetically homogeneous. Regions of dinucleotides were observed, most corresponding to (GT)n repeat motifs or (TG)n variations, as well as (AC)n, (CA)n, (AT)n and (AG)n motifs. The isolates analyzed in this study can be considered products of clonal lines, therefore no genetic diversity was found in these isolates.

The pepper weevil Anthonomus eugenii is one of the most damaging pests to the pepper crop. To offer alternative management strategies to insecticides, several studies have identified the semiochemicals that are involved in the pepper weevil’s aggregation and mating behavior; however, there is no information on its perireceptor molecular mechanism, to date. In this study, bioinformatics tools were used to functionally annotate and characterize the A. eugenii head transcriptome and their probable coding proteins. We identified twenty-two transcripts belonging to families related to chemosensory processes, seventeen corresponding to odorant-binding proteins (OBP), and six to chemosensory proteins (CSP). All results matched with closely related Coleoptera: Curculionidae homologous proteins. Likewise, twelve OBP and three CSP transcripts were experimentally characterized by RT-PCR in different female and male tissues. The results by sex and tissue display the different expression patterns of the AeugOBPs and AeugCSPs; some are present in both sexes and all tissues, while others show expressions with higher specificity, which suggests diverse physiological functions in addition to chemo-detection. This study provides information to support the understanding of odor perception in the pepper weevil.

Mexico produces more than four million tons of tomato fruits and ranks tenth worldwide. In February 2022, tomato plants in a greenhouse in Culiacan, Sinaloa State, were affected by wilt diseases with an incidence of 20% and irreversible wilt and death of the infected plants (severity up 70%). When cut stems from affected plants, a reddish to brown discoloration of the vascular system was observed and these were disinfected with 1% NaClO for 5 min and then placed in a humid chamber. Characteristic milky-white exudate was obtained. From that exudate, irregular, mucoid, and white colonies with pink centres were obtained on casamino peptone glucose (CPG) plates supplemented with 1% 2,3,5-triphenyl 15 tetrazolium chloride (TZC); these characteristics are typical of the Ralstonia solanacearum species complex (RSSC) (Garcia et al., 2019). Identification of the pathogen was done by PCR using specific primer pairs reported by Paudel et al. (2022), RssC-wF3 (5’-TATATATCCTCGACTTTTCCATGAAGCTGTG-3’) - RssCwR3 (5’-CTATATATATACCCCACTTGTTGAGGAACTG-3’) and Rpseu-wF5 (5’-TTTTATTTTTTTGGTGTCCGGGCCAAGATAG-3’) - Rpseu-wR5 (5’- TTATATTACTCGAACGTGCTGCAAAACCACT-3’), which amplified fragments of 162 and 251 bp for RSSC and Ralstonia pseudosolanacearum, respectively. Additionally, 759 (5’-GTCGCCGTCAACTCACTTTCC-3’) - 760 (5’-GTCGCCGTCAGCAATGCGGAATCG-3’) (Opina, et al., 1997) and Nmult21:1F (5′-CGTTGATGAGGCGCGCAATTT-3′) - Nmult22:RR (5’- TCGCTTGACCCTATAACGAGTA-3’) (Fegan and Prior, 2005) were used to generate 282 and 144 bp amplicons for RSSC and phylotype I, respectively. Subsequen to making the specific detection, the representative strain ClnMx was used to generate a sequence for the endoglucanase (egl) gene for separation into sequevars by using the primers Endo-F (5′- ATGCATGCCGCTGGTCGCCGC-3′) and Endo-R (5′-GCGTTGCCCGGCACGAACACC-3′), which amplified a fragment of 750 bp (Fegan et al., 1998). The egl sequence (GenBank Access ON542479) showed 100% identity with the well-defined R. pseudosolanacearum sequevar 14, which was isolated from tomato plants from Senegal (UW763, I-14 GenBank Access CP051174) (Steidl et al., 2021), as well as, the strain MAFF 301070 (GenBank Access AB508612) from Japanese tomato. For pathogenicity tests, four 1-month-old tomato plants were infected using an insulin syringe that contained a pure bacterial suspension with approximately 2x108 CFU/mL. For each plant, 20 µL was infiltrated into the axil of the third upper leaf, and for untreated controls, tomato plants were infiltrated with sterile water. All plants were kept at 28°C under greenhouse conditions. Symptoms resembling those observed in the field were observed in inoculated plants six days after inoculation, and the plant pathogen was recovered on TZC medium. To confirm the bacteria identification a PCR using the specific primer pairs mentioned early was carried out. In contrast, water-treated control plants remained healthy. Koch’s postulates were carried out twice with similar results. Ralstonia solanacearum species complex (RSSC) causes severe economic losses in many countries of the world because of their capability to infect a wide range of host plants, including potato, tomato, eggplant, tobacco, and, banana, among others. Ralstonia pseudosolanacearum has been reported to cause tomato wilt disease mainly on the Afro-Eurasian continent in areas such as Senegal, Cambodia, and Japan (Klass et al., 2019). To our knowledge, this is the first report of R. pseudosolanacearum causing bacterial wilt diseases in tomato plants from Mexico and because, the control of this bacteria is a challenge by the long survival time in soil, water, and infected plant tissues, the identification of this important pathogen could provide relevant information for developing management strategies.

Fungicide use is integral to reduce yield loss from Sclerotinia sclerotiorum on dry bean and soybean. Increasing fungicide use against this fungus may lead to resistance to the most common fungicides. Resistance has been reported in Brazil (Glycine max) and China (Brassica napus subsp. napus), however, few studies have investigated fungicide sensitivity of S. sclerotiorum in the U.S. The work presented here was conducted to determine if there was a difference in fungicide sensitivity of S. sclerotiorum isolates in the U.S. from: (i) dry bean versus soybean and (ii) fields with different frequencies of fungicide application. We further hypothesized that isolates with fungicide applications of a single active ingredient from tropical Brazil and sub-tropical Mexico were less sensitive compared to temperate U.S. isolates due to different management practices and climates. The EC50(D) fungicide sensitivity of 512 S. sclerotiorum isolates from the U.S. (443), Brazil (36), and Mexico (33) was determined using a discriminatory concentration (DC) previously identified for tetraconazole (2.0 ppm; EC50(D) ranged of 0.197 to 2.27 ppm), boscalid (0.2; 0.042 to 0.222), picoxystrobin (0.01; 0.006 to 0.027), and thiophanate-methyl, which had a qualitative DC of 10 ppm. Among the 10 least sensitive isolates to boscalid and picoxystrobin, two presented mutations known to confer resistance in the SdhB (qualitative) and SdhC (quantitative) genes; however no strong resistance was found. This study established novel DCs that can be used for further resistance monitoring and baseline sensitivity of S. sclerotiorum to tetraconazole throughout the world plus baseline sensitivity to boscalid in the U.S.

Pitahaya (Hylocereus spp.), also called dragon fruit, is a cultivated cactus that is native to Mexico as well as Central and South America. In October 2021, anthracnose symptoms were observed on fruit of pitahaya (Hylocereus costaricensis) in a commercial orchard located in Culiacán, Sinaloa, Mexico. Lesions on fruit were circular, sunken, dark brown and with halo. To fungal isolation, small pieces from adjacent tissue to lesions of symptomatic fruits were surface disinfested by immersion in a 2% sodium hypochlorite solution for 2 min, rinsed in sterile distilled water, and placed in Petri plates containing potato dextrose agar (PDA). The plates were incubated at 25 ºC for 5 days in darkness. Colletotrichum-like colonies were consistently observed on PDA and five monoconidial isolates were obtained. An isolate was selected as a representative for morphological identification, multilocus phylogenetic analysis, and pathogenicity tests. The isolate was deposited as CCLF186 in the Culture Collection of Phytopathogenic Fungi at the Research Center for Food and Development (Culiacán, Sinaloa). On PDA, initially white colonies turned grey with abundant orange conidia masses at 8 days after incubation at 25 ºC. Conidia were cylindrical, with ends rounded, aseptate, hyaline, and measuring 15.2 to 18.9 × 4.3 to 6.4 μm (n= 100). Appressoria were terminal, subglobose to clavate, of 7.4 to 11.6 × 5.9 to 8.2 µm (n= 30). Setae were not observed. These morphological characters were consistent with those reported for the Colletotrichum gloeosporioides species complex (Weir et al. 2012). To determine the phylogenetic identity of the isolate CCLF186, genomic DNA was extracted following the CTAB method (Doyle and Doyle 1990), and the internal transcribed spacer (ITS) region, the ApMat intergenic region, as well as partial sequences of actin (act) and glyceraldehyde-3-phosphate dehydrogenase (gapdh) genes were amplified and sequenced using the primers pairs ITS5/ITS4 (White et al. 1990), AM-F/AM-R (Silva et al. 2012), GDF/GDR, and ACT–512F/ACT–783R (Weir et al. 2012), respectively. The sequences were deposited in GenBank under accession nos. OP269659 (ITS), OP302778 (gapdh), OP302777 (act), and OP302779 (ApMat). BLASTn searches revealed high identity with sequences of C. tropicale (CBS 124949) for ITS (100%), ApMat (100%), act (100%), and gapdh (100%). A phylogenetic tree based on Bayesian inference and Maximum Likelihood methods, including published ITS, ApMat, act, and gapdh sequence datasets for isolates in the Colletotrichum gloeosporioides species complex was generated. The phylogenetic analysis based on the concatenated sequences clustered the isolate CCLF186 with the C. tropicale reference isolates. Pathogenicity of the isolate CCLF186 was confirmed on 10 healthy pitahaya fruits without wounds. A drop of a conidial suspension (1 × 105 spores/ml) was placed on two locations on each fruit. Ten control fruits were treated with sterilized water. The fruits were kept in a moist plastic chamber at 25°C and 12 h light/dark for 8 days. The pathogenicity test was repeated twice. All inoculated pitahaya fruits exhibited sunken and necrotic lesions 6 days after inoculation, whereas no symptoms were observed on the control fruits. The fungus was consistently re-isolated only from the diseased fruits and found to be morphologically identical to the isolate used for inoculation. Recently, C. tropicale causing anthracnose in dragon fruit (Selenicereus monacanthus) was reported from Philippines (Evallo et al. 2022). Now, this is the first report of C. tropicale causing fruit anthracnose in H. costaricensis in Mexico and worldwide. These findings provide a basis for research about the distribution and effective disease-management strategies.

Rhizoctonia solani, is the causal agent of black scurf and stem canker of potatoes (Solanum tuberosum L.) throughout the world. In November 2021, stem canker symptoms were observed in two potato fields located in Ahome, Sinaloa, Mexico. The disease incidence was estimated up to 15%. For fungal isolation, fragments of symptomatic stems were surface sterilized with 2% sodium hypochlorite for 2 min, rinsed with sterilized distilled water, and blotted dry on sterile filter paper. Fragments were placed on PDA medium and incubated at 25°C in darkness for 4 days. Rhizoctonia-like colonies were consistently obtained and 12 isolates were purified by the hyphal-tip method. Fungal colonies on PDA were white initially and then turned brown, raised, and with entire or undulate edges. Septate hyphae were hyaline, smooth, and branched at right angles with a septum near the point of branching. Microscopic examination by staining with 1% safranin O and 3% KOH solution showed multinucleate cells. The morphological features of the isolates resembled those of Rhizoctonia solani (Sneh et al. 1991). Four representative isolates were selected for molecular analysis and pathogenicity tests. The isolates were deposited in the Culture Collection of Phytopathogenic Fungi at the Research Center for Food and Development (Culiacán, Sinaloa) under accession nos. CCLF267, CCLF274, CCLF277, and CCLF279. For molecular identification, genomic DNA from each of the four isolates was extracted, and the internal transcribed spacer (ITS) region was amplified, and sequenced with the primer pair ITS5/ITS4 (White et al. 1990). The sequences were deposited in GenBank (accession nos. OP784258 to OP784261). Phylogenetic analyses were performed using the Maximum Likelihood method with ITS sequences for anastomosis groups (AG) of Rhizoctonia solani. The phylogenetic tree grouped the four isolates within the R. solani AG-7 clade with high bootstrap support (100%). For pathogenicity tests, certified pathogen-free potato mini-tuber (cv. Fianna) were placed in a polystyrene pot (1 L) filled with a 5 cm layer of a sterile substrate composed of soil and peat moss (2:1 w/w). One rice grain (20 mg) colonized with each isolate was placed 10 mm above the uppermost sprout tip and covered with the sterile substrate (Inokuti et al. 2019). Control plants were inoculated with sterile rice grains. All pots were transferred to a greenhouse where the temperature ranged from 20 to 32°C. Stem necrosis symptoms were observed on all inoculated plants 25 days after emergence, whereas control plants remained symptomless. Pathogenicity test was performed twice with similar results. Fungi were reisolated from the infected stems and found to be morphologically identical to the isolates used for inoculation, thus fulfilling Koch’s postulates. The AG-7 has been previously reported to cause potato diseases in South Africa (Truter and Wehner 2004). In Mexico, Carling et al. (1998) reported the presence of an isolate of R. solani AG-7 obtained from a potato tuber-borne sclerotium in Toluca; however, there is no information about the methodology used for the characterization of that isolate. To our knowledge, this is the first confirmed report of R. solani AG-7 causing potato stem canker in Mexico. Our findings improve knowledge about R. solani AGs occurring in potato fields in Mexico. So, further studies should be conducted to investigate the diversity, prevalence, and fungicide sensitivity of AGs distributed in the main potato-producing states in Mexico.

The sexual sensation seeking scale (SSSS) is a widely used instrument to measure individuals’ tendency to seek an optimal level of sexual arousal and novel sexual experiences. However, psychometric studies have suggested different factor structures for this instrument, which may lead to a biased assessment of the subdimensions of sexual sensation seeking. The present study (N = 812) aimed to identify the best factorial model of the SSSS by comparing the model suggested by previous research. Results from Exploratory and Confirmatory Factor Analyses showed that none of the models tested have sufficient goodness-of-fit to support the internal validity of the instrument. Thus, this study highlights the limitations of the SSSS in assessing sexual sensation seeking and proposes psychometric alternatives that should be considered by researchers to achieve a reliable measure of sexual sensation seeking. info:eu-repo/semantics/acceptedVersion

In November 2018, symptoms of brown rot were observed on chayote (

In November 2018, symptoms of brown rot were observed on chayote (Sechium edule) var. nigrum spinosum with a 20% disease incidence of 120 harvested fruits in the National Germplasm Bank of Sechium edule, located in the Centro Regional Universitario Oriente (CRUO) from the Chapingo Autonomous University (Huatusco, Veracruz, Mexico). For fungal isolation, pieces from symptomatic fruits were surface disinfected by immersion in a 1.5% NaClO solution for 2 min, rinsed in sterile distilled water, placed in Petri plates containing potato dextrose agar (PDA) amended with kanamycin sulfate, and incubated at 25ºC. Fusarium-like colonies were consistently isolated on PDA and five monoconidial isolates were obtained. A representative isolate was selected for morphological characterization, phylogenetic analysis, and pathogenicity tests. On PDA, colonies exhibited white and fluffy aerial mycelia, with diffused pale brown pigment in the center at 7 days of incubation at 25℃ in darkness. Macroconidia (n= 100) were hyaline, falcate, with 4 to 5 septa, measuring 23.9 to 31.9 × 2.9 to 4.2 μm, and foot-shaped basal cells. Microconidia and chlamydospores were absent. Morphological features were consistent with the description of the Fusarium incarnatum-equiseti species complex (Xia et al. 2019). The isolate was deposited as FUS2 in the Culture Collection of Phytopathogenic Fungi of the Laboratory of Plant Pathology at the Colegio de Postgraduados. For molecular identification, genomic DNA was extracted, and the internal transcribed spacer (ITS) region, partial sequences of translation elongation factor 1-alpha (EF1-α), and the second-largest subunit of RNA polymerase II (rpb2) genes were amplified, and sequenced with the primer sets ITS5/ITS4 (White et al. 1990), EF1-728F/EF1-986R (Carbone and Kohn 1999), and RBP2-5F/RPB2-7R (Liu et al. 1999), respectively. DNA sequences were edited in BioEdit 7.2 and compared with those in the NCBI nucleotide database. Alignments were implemented in MEGA X using reference sequences from Fusarium spp. A phylogenetic tree, including published ITS, EF1-α, and rpb2 sequence data, was constructed for the Fusarium incarnatum-equiseti species complex (FIESC) based on Maximum Likelihood. The sequences were deposited in GenBank (accession nos. ON878083, ON890421, and ON890420). The phylogenetic analysis grouped the isolate FUS2 within the F. citri clade. Pathogenicity of the fungus was verified on 10 healthy chayote fruits var. nigrum spinosum previously disinfested by immersion in a 1% NaClO solution for 3 min and washed in sterile water. A total of 5 mL of a conidial suspension (1 × 106 spores/ml) was sprayed on each whole fruit. Ten control fruit were sprayed with sterile distilled water. The fruits were kept in a moist plastic chamber at 25°C and 12 h light/dark for 30 days. All inoculated fruits developed water-soaked brown lesions (3 to 5 cm in diameter) covered with white mycelium at 15 days after inoculation, whereas no symptoms were observed on the control fruits. The fungus was consistently re-isolated only from the diseased fruits and found to be morphologically identical to the isolate used for inoculation, fulfilling Koch´s postulates. Fusarium citri has been associated with Capsicum sp. and mandarin orange in China, Triticum sp. in Iran, alfalfa in Denmark, and lettuce in the Czech Republic and Italy (Farr and Rossman 2022). To our knowledge, this is the first report of F. citri causing postharvest fruit rot of chayote in Mexico and worldwide.

Eggplant (

Objective: This PUBA study aims to evaluate the effectiveness of the Fractional CO2 Laser in the conservative treatment of Urinary Stress Incontinence in pre-selected Post-Menopausal Patients, with urine loss to Valsalva maneuvers. Methods: Symptoms of Urinary Stress Incontinence were evaluated before, and 1 month after the last session (3 sessions with a 30-day interval between them) in 50 post-menopausal women (Middle Age - Menopause, Interquartile Range Parity - BMI). Subjective measures (ICIQ SF, VAS) and objective measures such as PAD TEST were used (a dry diaper was previously weighed, and she was instructed to take 500 cc for 15 minutes at rest, and then for 45 minutes to perform Valsalva maneuvers, go up and down stairs, sit, stand up, etc. and then reweigh the diaper) during the study period in order to evaluate the results of the fractional CO2 laser treatment compared to the baseline. Results: Of the 50 patients enrolled, 12 patients in total who presented minimal Urinary symptoms of incontinence (USI) according to the ICIQ questionnaire, all were cured, defining this, as the no loss of urine. Of the 18 patients who presented moderate USI, 10 were cured (55%), that is, they did not present urine loss, and the other 8 patients (45%) had partial recovery, but continued losing urine. Of the 20 patients who presented severe USI, none were cured, 9 (45%) of them remained in severity, and 11 (55%) patients had partial recovery, migrating to the degree of moderate USI. An objective assessment was also performed using the PAD TEST, of the 12 patients with minimal USI, all were cured, of the 18 patients who presented moderate USI, what was found in the ICIQ was confirmed, with 10 patients cured, and 8 with partial recovery. Likewise, of the 20 patients with severe USI, none were cured. VAS (Visual Analogue Scale) was also performed at the end of the treatment and after a year by telephone call, to assess the degree of satisfaction, in regards to the minimal USI at the end of the treatment, this was 100%, and after a year it was reduced to 95%. The patients who presented moderate USI at the end of the treatment presented an average degree of satisfaction of 88.8%, and after 1 year it was 72.2%, and in respect of severe USI it was 6.5% at the end of the laser treatment, and after one year it was 0% Conclusions: In the present study, the data suggest that Fractional CO2 Laser is an effective alternative for the treatment of Minimal-Moderate Urinary Stress Incontinence in well-selected post-menopausal patients, with positive results that persist over time. Keywords (MESH): Fractional CO2 Laser; Menopause; Urinary Incontinence

Turcicum leaf blight (TLB), is a common foliar disease of maize in Mexico that is caused by the fungal pathogen

Insect chemosensory systems, such as smell and taste, are mediated by chemosensory receptor and non-receptor protein families. In the last decade, many studies have focused on discovering these families in Tephritidae species of agricultural importance. However, to date, there is no information on the Mexican fruit fly Anastrepha ludens Loew, a priority pest of quarantine importance in Mexico and other countries. This work represents the first effort to identify, classify and characterize the six chemosensory gene families by analyzing two head transcriptomes of sexually immature and mature adults of A. ludens from laboratory-reared and wild populations, respectively. We identified 120 chemosensory genes encoding 31 Odorant-Binding Proteins (OBPs), 5 Chemosensory Proteins (CSPs), 2 Sensory Neuron Membrane Proteins (SNMPs), 42 Odorant Receptors (ORs), 17 Ionotropic Receptors (IRs), and 23 Gustatory Receptors (GRs). The 120 described chemosensory proteins of the Mexican fruit fly significantly contribute to the genetic databases of insects, particularly dipterans. Except for some OBPs, this work reports for the first time the repertoire of olfactory proteins for one species of the genus Anastrepha, which provides a further basis for studying the olfactory system in the family Tephritidae, one of the most important for its economic and social impact worldwide.

Several endocrine manifestations have been described in patients with 22q11 deletion syndrome, including growth retardation, hypoparathyroidism, and thyroid disorders. This study aimed to characterize these abnormalities in a Colombian retrospective cohort of children with this condition.A retrospective study comprising a cohort of children with 22q11 deletion syndrome in Medellín, Colombia followed between 2011 and 2017 was conducted.Thirty-seven patients with a confirmed diagnosis of 22q11 deletion syndrome were included. 37.8% had some endocrinopathy, the most frequent being hypoparathyroidism (21.6%), followed by hypothyroidism (13.5%), hyperthyroidism (2.7%) and growth hormone deficiency (2.7%). There was wide heterogeneity in the clinical presentation, with late onset of severe hypocalcemia associated with seizure or precipitated in the postoperative cardiac surgery, which highlights the importance of continuous follow-up as indicated by the guidelines. Short stature was mainly related to nutritional factors. Growth monitoring is required with the use of syndrome-specific charts and careful monitoring of the growth rate.As previously reported, a significant proportion of patients with endocrine abnormalities were found in this cohort. This highlights that is essential to carry out an adequate multidisciplinary follow-up, based on the specific clinical guidelines, in order to avoid serious complications such as convulsions due to hypocalcemia. It is important to track size with curves specific to the syndrome and analyze the growth rate.

Common bean (

Sesame (Sesamum indicum L.: Pedaliaceae) is the second most cultivated oilseed in Mexico with 80,000 ha per year. The seeds of this crop are used as a condiment, for the extraction of oil, and its medicinal properties. In October 2020, collar rot symptoms were observed in six sesame fields (SOPC-9539 TD variety) located in the Carrizo Valley (26°15'33.1"N; 109°01'37.9"W), El Fuerte, Sinaloa, México. Initially, small brown spots in the basal stem of the infected plants were observed. At advanced stages of the disease, the circumference of stem was necrotic with the presence of white mycelium that extends to the roots. Infected plants were showing symptoms of yellowing, wilting, and finally death. Disease incidence was estimated at 15%, counting the total of diseased plants in five counts done in arbitrary quadrants within the sesame fields. For fungal isolation, stem sections from the symptomatic basal stem were surface disinfected with 1.5% sodium hypochlorite for 2 min, then triple rinsed with sterile distilled water. The tissue sections were dried on sterile blotting paper and plated in Petri dishes with potato dextrose agar (PDA) culture medium. The plates were incubated at 28ºC in darkness for 48 h. Sclerotinia-like colonies were consistently isolated and four isolates from different locations were purified by the hyphal-tip method. Fungal colonies were formed of compact white mycelium, with the formation of sclerotia on the margin of the plate 6 days after inoculating PDA cultures. Sclerotia averaged 3.1 mm in diameter and 0.024 g. One isolate was deposited in the Culture Collection of Phytopathogenic Fungi of the Faculty of Agriculture of Fuerte Valley at the Sinaloa Autonomous University under Accession no. FAVF654. To confirm identification, genomic DNA was extracted from one isolate, and the internal transcribed spacer (ITS) region was amplified by PCR and sequenced directly using the primer pair ITS5/ITS4 (White et al. 1990). The resulting consensus sequence was deposited in GenBank under accession no. ON401416. BLASTn alignments in GenBank showed 100% identity of our sequence with the sequence of the type strain of Sclerotinia sclerotiorum ATCC 46762 (accession no. JX648201). Pathogenicity of the fungus was demonstrated by inoculating healthy sesame plants (Dormilón and SOPC-9539 TD ies), germinated in plastic pots with sterile substrate. Plants were inoculated with the FAVF654 isolate by applying 3 sclerotia at the base of each of the 12 plants. Twelve plants were left uninoculated, which served as controls. All the inoculated plants, of both varieties, developed the characteristic symptoms of the disease 7 days after inoculation, while the control plants remained symptomless. The pathogenicity test was performed twice with the same result. The fungus was reisolated from all the inoculated plants, thus fulfilling Koch's postulates. Sclerotinia sclerotiorum has been reported on sesame plants in Bulgaria and Korea (Farr and Rossman, 2022). To our knowledge, this is the first report of Sclerotinia sclerotiorum causing collar rot in sesame plants in Mexico and the Americas. This disease considerably reduces the yield of sesame; therefore it is necessary to develop effective disease-management strategies.

Insect chemosensory systems, such as smell and taste, are mediated by chemosensory receptor and non-receptor protein families. In the last decade, many studies have focused on discovering these families in Tephritidae species of agricultural importance. However, to date, there is no information on the Mexican fruit fly

Nanche (Byrsonima crassifolia) has great economic importance in the dry and humid tropics in Mexico, where is used for the elaboration of different drinks, foods, and candies; also in the traditional medicine to treat fever and stomach disorders. Since 2021, a high incidence of disease-causing scabby fruit in young nanche fruits has been detected in Las Choapas, Veracruz, Mexico. Disease symptoms were visible on the skin of fruits which progressed as fruits matured. Therefore, the objectives of this study were to identify the causal agent of this scab disease based on morphology and molecular techniques and to determine their pathogenicity. Fifteen isolates were recovered from diseased fruits. Because the isolates presented similar morphology, two of them were selected to determine their identity and pathogenicity. On the basis of colony characteristics, conidial morphology, and phylogenetic analyses of the internal transcribed spacers, partial translation elongation factor 1-α gene, and partial β-tubulin gene sequence the isolates were identified as Neopestalotiopsis australis. Koch's postulates demonstrated the pathogenicity of N. australis on nanche. This is the first report implicating N. australis as a causal agent of Scab Disease on B. crassifolia in Mexico and in the world.

Las luciérnagas (Coleoptera: Lampyridae) tienen importancia turístico-cultural debido a su bioluminiscencia. En el “Santuario de las Luciérnagas” (Nanacamilpa, Tlaxcala) habitan poblaciones de Photinus palaciosi. Anualmente, este sitio es visitado por una gran cantidad de turistas. Sin embargo, faltan estudios ecológicos sobre la biología de la especie y factores abióticos que afectan la abundancia de sus poblaciones. Se comparó la abundancia de adultos en 3 tipos de vegetación y se analizó la relación de factores abióticos sobre la abundancia local de esta especie, se registró talla promedio y proporción de sexos. Se hicieron muestreos en bosque de encino, pino y mixto (pino-encino), con redes aéreas. Se recolectaron 397 especímenes (386 machos y 11 hembras). La temperatura, humedad, punto de rocío y el índice de calor, afectaron la abundancia de las poblaciones de la especie. No hubo un efecto de la vegetación sobre la abundancia y el tamaño corporal. La proporción sexual mostró un sesgo hacia los machos y diferencias entre el bosque de encino (frecuencia menor a la esperada) y el mixto presentó un patrón contrario. Es necesario seguir con estudios a largo plazo para comprender el estado de las poblaciones y proponer medidas de conservación.

Two field experiments were conducted to evaluate synthetic attractants derived from pepper flowers, flower buds and fruits, alone or in combination with the aggregation pheromone. The evaluation was carried out with the release and recapture of Anthonomus eugenii adults at different distances from the four cardinal points in separate trials. The volatility of the synthetic mixture and aggregation pheromone was determined by gas chromatographic analysis of the volatiles captured by dynamic headspace. The traps with synthetic mixture and essential oil captured insects at 10 m, while the aggregation pheromone trapped up to 60 m. The combination of synthetic mixture or essential oil with the aggregation pheromone did not increase the number of recaptures compared to the single pheromone. The synthetic mixture together with geranic acid recaptured adults up to 15 m, although they were not significantly different from the control. The exclusion of geranic acid from the aggregation pheromone significantly reduced the number of recaptured insects (P

La relación entre el deseo y el cuerpo como territorio de control para la constitución de las ficciones políticas de feminidad y masculinidad exige el estudio de los postulados del siglo xx y su evolución contrastada con lecturas contemporáneas. Proponemos, así, las categorías de psicoanálisis Freudiano con la patologización del deseo femenino y el poder institucional de Foucault. Serán revisadas las visiones en virtud de la irrupción del fenómeno queer, lo performativo del género en Butler, el imperio excluyente de la heterónoma a partir de la relación consecuente entre sexo-género-deseo y las lecturas sociológicas de Connell y Sara Martín en la construcción identitaria de la masculinidad viril. Por último, en el intento de vislumbrar una episteme alternativa, se propone el modelo farmocopornográfico de Preciado, que converge dos industrias: la farmacéutica, puntualizando el análisis en la píldora anticonceptiva, y la pornográfica, como instrumento semiótico para la construcción y manipulación del placer. Los dispositivos biotecnológicos abordados permitirán transitar de las lógicas socioculturales al lente de lo bioquímico, concluyendo que lo molecular también es político.

Background: The present retrospective study reviewed acute promyelocytic leukemia (APL) cases recorded in Mexico between January 2007 and January 2017. The primary objective of the study was to evaluate overall survival (OS) in Mexican patients with APL. Secondary objective was to evaluate the impact of induction treatment with different anthracyclines on OS, event-free survival (EFS) and complications in this patient population. Methods: The medical charts of patients referred to medical institutions in Mexico from January 2007 through January 2017 for the treatment of suspected APL were reviewed retrospectively. Patients aged 15 - 75 years, in whom the diagnosis of APL was confirmed, who had an Eastern Cooperative Group performance status of 0 - 2, and who were eligible for combined treatment with intensive chemotherapy and all-trans retinoic acid (ATRA), were included in the study. Study participants received induction and consolidation treatment with ATRA plus either daunorubicin or idarubicin, followed by 2 years of single-agent ATRA as maintenance therapy. Patients who were unable to pay for ATRA treatment received anthracycline-based induction and consolidation, with methotrexate plus mercaptopurine as maintenance therapy. Results: A total of 360 patients from 21 public and private hospitals were included in the study. The median age of the population was 37 years, and 51% were male. Of the 360 patients, 205 (57%) vs. 155 (43%) received daunorubicin vs. idarubicin as induction treatment for APL. ATRA was administered to 201 (98%) patients in the daunorubicin group vs. 138 (89%) in the idarubicin group (P = 0.001), and was initiated at diagnosis in 92% vs. 73% of recipients, respectively (P = 0.0001). At 150 months, OS and EFS for the entire population were 84% and 79%, respectively. Both OS (90% vs. 76%, P = 0.003) and EFS (85% vs. 72%, P = 0.001) were significantly prolonged in daunorubicin vs. idarubicin recipients. Rates of complications were similar in the two groups. Conclusions: As arsenic trioxide (ATO) is not currently available in Mexico, anthracycline plus ATRA is the mainstay of treatment for APL here. Our results confirm the efficacy of this strategy, with high OS and EFS rates being observed 12.5 years after diagnosis. J Hematol. 2021;10(2):53-63 doi: https://doi.org/10.14740/jh773

Pitahaya (

Anthracnose, caused by

Tomato is the most economically important vegetable crop worldwide and the second most important for Mexico. However, viral diseases are among the main limiting factors that affect the productivity of this crop, causing total losses in some cases. This review provides key information and findings on the symptoms, distribution, transmission, detection, and management of diseases caused by viruses of major importance in tomato crops in Mexico. Currently, about 25 viruses belonging to nine different families have been reported infecting tomato in Mexico, but not all of them cause economically significant diseases. Viruses of economic importance include tomato brown rugose fruit virus (ToBRFV), tomato spotted wilt virus (TSWV), tomato yellow leaf curl virus (TYLCV), pepino mosaic virus (PepMV), and tomato marchitez virus (ToMarV). The topics discussed here will provide updated information about the status of these plant viruses in Mexico as well as diverse management strategies that can be implemented according to the specific circumstances of each viral pathosystem. Additionally, a list of tomato-affecting viruses not present in Mexico that are continuous threats to the crop health is included.

La muerte descendente del limón ‘Persa’ (Citrus latifolia Tan.) causada por Lasiodiplodia spp., es una enfermedad de alta importancia. La intensidad de las podas en limón incrementa la susceptibilidad y la pérdida anual de hasta 35% de árboles, lo que resulta 60% de reducción de la producción. Durante los años 2016 y 2017, en el municipio de Papantla, Veracruz se evaluó la efectividad del control químico y biológico contra Lasiodiplodia spp., después de la poda. Se realizó un diseño de bloques completo al azar, se aplicaron cinco tratamientos: metil tiofanato (1 g L-1), tiabendazole (2.5 g L-1), clorotalonil (3 g L-1) y mancozeb (4 g L-1), Trichoderma harzianum + Trichoderma viridae (20 ml L-1) y un testigo (sin aplicación). Se realizaron dos aspersiones dirigidas a tronco, ramas y follaje hasta punto de goteo. Se evalúo la incidencia y severidad de la enfermedad cada siete días después de la primera aplicación. Con los datos de incidencia se realizó ajuste de modelos epidemiológicos y se calcularon los parámetros descriptivos como: intensidad inicial, tasa de incremento y área bajo la curva del progreso de la enfermedad. El fungicida metil tiofanato tuvo mayor efectividad para el control de la enfermedad, seguido de tiabendazol.

Anthracnose, caused by Colletotrichum spp., is the most significant field and post-harvest disease of mango worldwide and is mainly controlled through the use of systemic fungicides belonging to the methyl benzimidazole carbamate (MBC) class. In Mexico, this disease in mango has been associated with at least seven cryptic species belonging to the C. gloeosporioides complex. In this study, the distribution of sensitivity to MBC (benomyl and carbendazim) and prochloraz fungicides of 101 Colletotrichum spp. isolates obtained from 101 commercial orchards in Mexico’s most important mango-producing states was determined. All Colletotrichum spp. isolates were obtained from commercial mango orchards with applications of MBC fungicides and that had not been exposed to applications of prochloraz, or any other DMI (demethylation inhibitors) class fungicide. Isolates were evaluated using an in vitro test to determine the effective fungicide concentration at which 50% of mycelial growth is inhibited (EC50) for each isolate-fungicide combination. EC50 values for benomyl and carbendazim ranged from 0.0967 to 0.3995 µg mL−1 (median = 0.1923 µg mL−1) and from 0.0880 to 0.1703 µg mL−1 (median = 0.1171 µg mL−1), respectively, while those for prochloraz ranged from 0.0066 to 0.0813 µg mL−1 (median = 0.0253 µg mL−1). Our results revealed a wide distribution in Mexico of Colletotrichum spp. isolates from mango sensitive to MBC fungicides and prochloraz. Data obtained in this study will serve as a source of comparison of the evolution of the sensitivity of Colletotrichum spp. populations obtained from mango to MBC and DMI fungicides in the future.

Passive acoustic monitoring is a technique in which acoustic sensors are placed with a particular recording setting, without interference from the observer. Despite the recent interest, many places in the Neotropics still do not have information on acoustic activity. Such acoustic sampling events would contribute to answer different questions about the acoustic diversity in the Neotropics. We present the dataset of passive acoustic sampling events during the first semester of 2020, at the Natural Reserve Los Yátaros, 6 km away of Gachantivá, Boyacá, Eastern Andes of Colombia. Sampling consisted of installing six AudioMoth acoustic sensors, two at each sampling site (n=3). We programmed all acoustic sensors to record 1 min every 30 minutes throughout the day (00:00-23:30) to audible spectrum (0 Hz-16 kHz), and at night (16:30-06:00) to ultrasound spectrum (up to 192 kHz). The monitoring was conducted from March 1 to May 2, 2020, gathering 12 447 recording, 9055 audible and 3392 ultrasound recordings.

Contexto: A partir de un análisis desarrollo de la tecnología en biodigestores, se observó un problema recurrente en la cuantificación rigurosa del metano. mediante una revisión bibliográfica reveló sistemas poco eficientes o de costos elevados. Este proyecto plantea un sistema accesible y eficaz que busca contribuir a proyectos académicos y de investigación afines. Método: La cuantificación de metano se realiza mediante un sensor de la serie MQ-4 acoplado a una cápsula, creando un medio parcialmente cerrado en el que se inyecta, por medio de una jeringa, una dilución de gases de muestra de metano con aire del ambiente, lo cual amplía el rango de medición del sensor. Resultados: Se elaboró una curva de calibración, ampliando el rango de medición de 0,02-1 % de concentración a 10-80 %v/v, con una varianza de ± 5 %. Conclusiones: Se desarrolló un prototipo de cuantificación de metano eficiente y accesible para uso académico y de investigación. Esto se complementa con una guía para replicar el proceso.

O Transtorno do Jogo (TJ) é considerado atualmente como um transtorno de dependência (DSM-5) por ter caraterísticas similares com transtornos deste tipo como os causados por uso de substâncias, tendo prejuízos para a vida do individuo e para a sociedade como um todo. Sua prevalência é significativa no mundo variando entre 0,2 e 5,8%, sendo que no Brasil é de 1%, e tendo as loterias como uma das formas mais populares de jogo (controladas pela Caixa Econômica Federal), por ser uma das poucas formas legalizadas ( junto com corridas e pôquer). Assim, varias ferramentas de rastreio tem sido desenvolvidas, dentro das quais se destaca a NODS-CLiP pelo seu desempenho e de fácil aplicação; Porém, esta escala junto com as demais desenvolvidas até hoje tem surgido da pesquisa em populações de universitários ou em amostras clínicas em diferentes países sem incluir o Brasil. Pelo exposto, o presente estudo tem como principal objetivo encontrar a melhor forma de rastreio no território nacional e na comunidade. Para isto foi realizada uma pesquisa de tipo transversal em uma população com pelo menos 5.000 entrevistas aos apostadores da caixa (cálculo realizado para uma boa representatividade), distribuídas pelo país inteiro em 500 unidades lotéricas. Os entrevistadores foram treinados para realizar o questionário completo da NODS (National Opinion Research Center for DSM Screen for Gambling Problems) usando os critérios DSM-5, além da coleta de dados demográficos. No total 23.123 indivíduos foram abordados, dos quais 5.407 aceitaram participar e terminaram a entrevista. Como resultado, observou-se que o apostador médio era homem, com 50,2 anos de idade, casado, com pelo menos ensino médio incompleto e com carteira de trabalho ou autônomo. Os sintomas mais frequentes nesta população foram preocupação e uso de jogo como forma de aliviar emoções negativas. Três combinações de dois, três e quatro itens da NODS foram identificadas como as melhores formas de identificar clientes de loteria com TJ e Jogo Problemático (JPR) . Resultando que tanto ao longo da vida e nos últimos 12 meses a combinação de três itens teve um desempenho melhor que a da NODS-CLiP; Este conjunto de três itens incluía: i) controle; ii) jogar para lidar com as emoções negativas (escapismo); e iii) jogar para recuperar. Quando analisados esses resultados, levantou-se a hipótese de que a prevalência masculina pode estar associada a fatores aquisitivos dada a desigualdade social, ainda existente entre ambos os gêneros. A prevalência de TJ e JPR foi maior entre jogadores de loteria se compararmos com as prevalências na população brasileira, pelo que pode ser uma ambiente ideal de aplicação de escalas de rastreio. O melhor desempenho da escala de três itens proposta neste estudo, com melhor desempenho do que a NODS-CLiP, pode ser explicada, talvez, pela combinação intrínseca entre cultura brasileira e natureza própria do TJ, que fazem com que itens diferentes sejam melhores para rastreio de jogo na população do país e na comunidade. Finalmente o desenvolvimento deste instrumento pode ser uma ferramenta forte no âmbito da saúde pública nacional ao poder identificar um transtorno relativamente frequente na atenção primária Gambling Disorder (GD) is currently considered as an addiction disorder (DSM-5) because of the many similarities with those as for example substance abuse, besides it brings damage to the individual\'s life and to society as a whole. Its prevalence is significant around the world, ranging between 0.2 and 5.8%, in Brazil it is 1%, and with lotteries as one of the most popular types of gambling (controlled by Caixa Econômica Federal), as it is one of the few legal forms (along with racing and poker). Thus, several screening tools have been developed, among which NODS-CLiP stands out for its performance and easy application; However, this scale, together with others developed so far, has emerged from research in university populations or in clinical samples in different countries, without including Brazil. For these reasons, the main goal of this study is to find the best form of screening in the national territory and in a community sample. For this, a cross-sectional survey was carried out in a population with at least 5,000 interviews within lotteries players (calculation performed for a better representation), distributed throughout the country in 500 lottery units. The interviewers were trained to complete the NODS (National Opinion Research Center for DSM Screen for Gambling Problems) questionnaire using the DSM-5 criteria in addition to demographic data collection. A total of 23,123 individuals were approached, of which 5,407 agreed to participate and ended the interview. As a result, it was observed that the average gambler was a man, aged 50.2 years, married, with at least incomplete high school education and with an informal or formal job. The most frequent symptoms in this population were preoccupation and the use of gambling when feeling distressed. Three combinations of two, three, and four NODS items were identified as the best ways to identify lottery customers with GD and Problem Gaming (PG). Resulting that the three-item combination performed better than the NODS-CLiP lifelong and in the last year; This set of three items included: i) control; ii) gamble when feeling distressed; and iii) chasing. When these results were analyzed, was raised that male prevalence may be associated with acquisitive factors given the social inequality, which still exists between both genders. The prevalence of GD and PG was higher among lottery players when compared to the prevalence in the Brazilian population, so it can be an ideal environment for the application of screening scales. The better performance of the three-item scale proposed in this study, with better performance than the NODS-CLiP, can be explained, perhaps, by the intrinsic combination between Brazilian culture and the nature of the GD, which makes different items better for Gambling screening in Brazil as well as in the community. Finally, the development of this instrument can be a strong tool in the scope of national public health, as it can identify a relatively frequent disorder in primary care