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The current research work attempted to investigate, for the first time, the impact of biochar addition, on anaerobic digestion of olive mill wastewater with different initial chemical oxygen demand loads in batch cultures (10 g/L, 15 g/L, and 20 g/L). Methane yields were compared by applying one-way analysis of variance (ANOVA) followed by post-hoc Tukey’s analysis. The results demonstrated that adding at 5 g/L biochar to olive mill wastewater with an initial chemical oxygen demand load of 20 g/L increased methane yield by 97.8% and mitigated volatile fatty acid accumulation compared to the control batch. According to the results of microbial community succession revealed by the Illumina amplicon sequencing, biochar supplementation significantly increased diversity of the microbial community and improved the abundance of potential genera involved in direct interspecies electron transfer, including Methanothrix and Methanosarcina. Consequently, biochar can be a promising alternative in terms of the recovery of metabolic activity during anaerobic digestion of olive mill wastewater at a large scale. This study was supported by financial aids from ARIMNet2 PYRODI-GEST project under grant agreement no. 618127. Scopus

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Thyroid cancer is a complex disease where many factors influence its morbidity and mortality. Analyzing the clinic pathological parameters of this malignant disease in a series of thyroid cancer patients allows to evaluate their clinical behavior and outcomes. A retrospective analysis on clinical and pathological data of 92 patients with thyroid cancer was carried. Our results indicate that the mean age of the patients was 49.3 ± 14.5, that women accounted for 79.4% of the patients, and the mean followup duration was 67.4 months (range: 7–216 months). Papillary carcinoma was the most common histological type, with 84 cases (91.3%). Low pathological tumor-node-metastasis stage was observed in 39.4% of the patients. Ten year survival was 21.3%. Older patients were associated with advanced stage (stage III and IV) and with high recurrence rate. Understanding better how certain clinic pathologic variables influence disease progression in specific population groups has the potential to allow clinicians to tailor diagnostic and therapeutic options.