1. Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress—A pilot study
- Author
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Fahimeh Koohnavard, Mahsa Archang, Rodney B. Luwor, Zahra Khashavi, Ashok Agarwal, Ehsan Kamrani, Marcella Spinaci, Ahmad Homaei, Ralf Henkel, Khashavi Z., Homaei A., Koohnavard F., Kamrani E., Spinaci M., Luwor R.B., Archang M., Agarwal A., and Henkel R.
- Subjects
Male ,DNA damage ,Cell Survival ,Urology ,Semen ,Pilot Projects ,Semen analysis ,medicine.disease_cause ,cryopreservation ,Cryopreservation ,Male infertility ,Andrology ,Endocrinology ,medicine ,Animals ,Holothuria ,Humans ,Sperm motility ,Holotheria parva ,medicine.diagnostic_test ,urogenital system ,Chemistry ,Tissue Extracts ,ROS ,General Medicine ,Free Radical Scavengers ,medicine.disease ,Sperm ,Spermatozoa ,Chromatin ,Semen Analysis ,Oxidative Stress ,Sperm Motility ,Reactive Oxygen Species ,Oxidative stress ,DNA Damage ,Semen Preservation - Abstract
Cryopreservation is the most effective method for preserving semen for a long period of time. However, during the freeze–thaw process, production of reactive oxygen species (ROS) leads to a steep reduction in sperm fertility indices. In this study, we tested the effects of the extract of the coelomic cavity of five Holotheria parva, a marine organism rich in antioxidants, for its ROS-scavenging activity and cryoprotective effects on oxidative stress. Using a total of 50 semen samples, our results demonstrated that doses of 250 and 500µg/ml of H.parva coelomic cavity extract significantly increased sperm vitality as compared to the control (p 
- Published
- 2020