9 results on '"Liu, Chung-Ming"'
Search Results
2. Biofabrication of advancedin vitro3D models to study ischaemic and doxorubicin-induced myocardial damage
- Author
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Sharma, P, Liu Chung Ming, C, Wang, X, Bienvenu, LA, Beck, D, Figtree, G, Boyle, A, and Gentile, C
- Subjects
Doxorubicin ,Myocardium ,Myocardial Infarction ,Humans ,0903 Biomedical Engineering, 1004 Medical Biotechnology, 1099 Other Technology ,Heart ,Myocytes, Cardiac ,RNA, Messenger ,Cardiotoxicity - Abstract
Current preclinicalin vitroandin vivomodels of cardiac injury typical of myocardial infarction (MI, or heart attack) and drug induced cardiotoxicity mimic only a few aspects of these complex scenarios. This leads to a poor translation of findings from the bench to the bedside. In this study, we biofabricated for the first time advancedin vitromodels of MI and doxorubicin (DOX) induced injury by exposing cardiac spheroids (CSs) to pathophysiological changes in oxygen (O2) levels or DOX treatment. Then, contractile function and cell death was analyzed in CSs in control verses I/R and DOX CSs. For a deeper dig into cell death analysis, 3D rendering analyses and mRNA level changes of cardiac damage-related genes were compared in control verses I/R and DOX CSs. Overall,in vitroCSs recapitulated major features typical of thein vivoMI and drug induced cardiac damages, such as adapting intracellular alterations to O2concentration changes and incubation with cardiotoxic drug, mimicking the contraction frequency and fractional shortening and changes in mRNA expression levels for genes regulating sarcomere structure, calcium transport, cell cycle, cardiac remodelling and signal transduction. Taken together, our study supports the use of I/R and DOX CSs as advancedin vitromodels to study MI and DOX-induced cardiac damge by recapitulating their complexin vivoscenario.
- Published
- 2022
3. Stem Cell-Based 3D Bioprinting for Cardiovascular Tissue Regeneration
- Author
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Clara Liu Chung Ming, Eitan Ben-Sefer, and Carmine Gentile
- Published
- 2022
4. Towards engineering heart tissues from bioprinted cardiac spheroids
- Author
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Wafa Al Shamery, Fabian Haeusermann, Elysse C. Filipe, Hadi Mahmodi Sheikh Sarmast, Jelena Rnjak-Kovacina, Poonam Sharma, Carmine Gentile, Eitan Ben-Sefer, Maryam Alsadat Rad, Joanne L. Tipper, Hien A. Tran, Thomas R. Cox, Lydia Surija, Irina V. Kabakova, Florian Richter, Min Ho Lee, Laura Vettori, Clara Liu Chung Ming, and Liudmila Polonchuk
- Subjects
Vascular Endothelial Growth Factor A ,food.ingredient ,Biomedical Engineering ,Bioengineering ,Biochemistry ,Gelatin ,Biomaterials ,chemistry.chemical_compound ,food ,In vivo ,otorhinolaryngologic diseases ,Myocyte ,Humans ,0903 Biomedical Engineering, 1004 Medical Biotechnology, 1099 Other Technology ,Tissue Engineering ,Tissue Scaffolds ,Spheroid ,Bioprinting ,Endothelial Cells ,Hydrogels ,General Medicine ,In vitro ,Vascular endothelial growth factor ,Endothelial stem cell ,chemistry ,Self-healing hydrogels ,Printing, Three-Dimensional ,Biotechnology ,Biomedical engineering - Abstract
Current in vivo and in vitro models fail to accurately recapitulate the human heart microenvironment for biomedical applications. This study explores the use of cardiac spheroids (CSs) to biofabricate advanced in vitro models of the human heart. CSs were created from human cardiac myocytes, fibroblasts and endothelial cells (ECs), mixed within optimal alginate/gelatin hydrogels and then bioprinted on a microelectrode plate for drug testing. Bioprinted CSs maintained their structure and viability for at least 30 d after printing. Vascular endothelial growth factor (VEGF) promoted EC branching from CSs within hydrogels. Alginate/gelatin-based hydrogels enabled spheroids fusion, which was further facilitated by addition of VEGF. Bioprinted CSs contracted spontaneously and under stimulation, allowing to record contractile and electrical signals on the microelectrode plates for industrial applications. Taken together, our findings indicate that bioprinted CSs can be used to biofabricate human heart tissues for long term in vitro testing. This has the potential to be used to study biochemical, physiological and pharmacological features of human heart tissue.
- Published
- 2021
5. Characterisation of Cardiac Health in the Reduced Uterine Perfusion Pressure Model and a 3D Cardiac Spheroid Model, of Preeclampsia
- Author
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Zoran Cakic, Kristine C.Y. McGrath, Zeljko Mikovic, Chris Evenhuis, Valentina N. Nikolic, Claire Rennie, Milan Stefanovic, Lana McClements, Kimberly Sesperez, Kristen J. Bubb, Sahar Ghorbanpour, Carmine Gentile, Clara Liu Chung Ming, Natasa Karadzov Orlic, Claire Richards, and Michael Chhor
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Physiology ,Placenta ,Pregnancy Complications, Cardiovascular ,030204 cardiovascular system & hematology ,Preeclampsia ,Gender Studies ,Rats, Sprague-Dawley ,Tacrolimus Binding Proteins ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,FKBPL ,Pre-Eclampsia ,Fibrosis ,Pregnancy ,Internal medicine ,medicine ,QP1-981 ,Cardiac spheroids ,Animals ,Humans ,RNA, Messenger ,Kidney ,Sex Characteristics ,business.industry ,Research ,Endothelial Cells ,Cardiovascular disease ,medicine.disease ,Brain natriuretic peptide ,Reduced uterine perfusion pressure ,Rats ,Perfusion ,030104 developmental biology ,medicine.anatomical_structure ,Blood pressure ,embryonic structures ,Medicine ,Female ,Collagen ,business ,Artery - Abstract
Background Preeclampsia is a dangerous cardiovascular disorder of pregnancy that leads to an increased risk of future cardiovascular and metabolic disorders. Much of the pathogenesis and mechanisms involved in cardiac health in preeclampsia are unknown. A novel anti-angiogenic protein, FKBPL, is emerging as having a potential role in both preeclampsia and cardiovascular disease (CVD). Therefore, in this study we aimed to characterise cardiac health and FKBPL regulation in the rat reduced uterine perfusion pressure (RUPP) and a 3D cardiac spheroid model of preeclampsia. Methods The RUPP model was induced in pregnant rats and histological analysis performed on the heart, kidney, liver and placenta (n ≥ 6). Picrosirius red staining was performed to quantify collagen I and III deposition in rat hearts, placentae and livers as an indicator of fibrosis. RT-qPCR was used to determine changes in Fkbpl, Icam1, Vcam1, Flt1 and Vegfa mRNA in hearts and/or placentae and ELISA to evaluate cardiac brain natriuretic peptide (BNP45) and FKBPL secretion. Immunofluorescent staining was also conducted to analyse the expression of cardiac FKBPL. Cardiac spheroids were generated using human cardiac fibroblasts and human coronary artery endothelial cells and treated with patient plasma from normotensive controls, early-onset preeclampsia (EOPE) and late-onset preeclampsia (LOPE); n = 3. FKBPL and CD31 expression was quantified by immunofluorescent labelling. Results The RUPP procedure induced significant increases in blood pressure (p < 0.001), collagen deposition (p < 0.001) and cardiac BNP45 (p < 0.05). It also induced a significant increase in cardiac FKBPL mRNA (p < 0.05) and protein expression (p < 0.01). RUPP placentae also exhibited increased collagen deposition and decreased Flt1 mRNA expression (p < 0.05). RUPP kidneys revealed an increase in average glomerular size (p < 0.05). Cardiac spheroids showed a significant increase in FKBPL expression when treated with LOPE plasma (p < 0.05) and a trend towards increased FKBPL expression following treatment with EOPE plasma (p = 0.06). Conclusions The rat RUPP model induced cardiac, renal and placental features reflective of preeclampsia. FKBPL was increased in the hearts of RUPP rats and cardiac spheroids treated with plasma from women with preeclampsia, perhaps reflective of restricted angiogenesis and inflammation in this disorder. Elucidation of these novel FKBPL mechanisms in cardiac health in preeclampsia could be key in preventing future CVD.
- Published
- 2020
6. Biofabrication of advanced in vitro 3D models to study ischaemic and doxorubicin-induced myocardial damage
- Author
-
Poonam Sharma, Clara Liu Chung Ming, Xiaowei Wang, Laura A Bienvenu, Dominik Beck, Gemma Figtree, Andrew Boyle, and Carmine Gentile
- Subjects
Biomaterials ,Biomedical Engineering ,Bioengineering ,General Medicine ,Biochemistry ,Biotechnology - Abstract
Current preclinical in vitro and in vivo models of cardiac injury typical of myocardial infarction (MI, or heart attack) and drug induced cardiotoxicity mimic only a few aspects of these complex scenarios. This leads to a poor translation of findings from the bench to the bedside. In this study, we biofabricated for the first time advanced in vitro models of MI and doxorubicin (DOX) induced injury by exposing cardiac spheroids (CSs) to pathophysiological changes in oxygen (O2) levels or DOX treatment. Then, contractile function and cell death was analyzed in CSs in control verses I/R and DOX CSs. For a deeper dig into cell death analysis, 3D rendering analyses and mRNA level changes of cardiac damage-related genes were compared in control verses I/R and DOX CSs. Overall, in vitro CSs recapitulated major features typical of the in vivo MI and drug induced cardiac damages, such as adapting intracellular alterations to O2 concentration changes and incubation with cardiotoxic drug, mimicking the contraction frequency and fractional shortening and changes in mRNA expression levels for genes regulating sarcomere structure, calcium transport, cell cycle, cardiac remodelling and signal transduction. Taken together, our study supports the use of I/R and DOX CSs as advanced in vitro models to study MI and DOX-induced cardiac damge by recapitulating their complex in vivo scenario.
- Published
- 2022
7. Considerations for the Bioengineering of Advanced Cardiac In Vitro Models of Myocardial Infarction
- Author
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Andrew J. Boyle, Laura Vettori, Poonam Sharma, Gemma A. Figtree, Clara Liu Chung Ming, Carmine Gentile, and Xiaowei Wang
- Subjects
medicine.medical_specialty ,Ischemia ,Myocardial Infarction ,Bioengineering ,Myocardial Reperfusion Injury ,02 engineering and technology ,Biology ,010402 general chemistry ,01 natural sciences ,law.invention ,Biomaterials ,law ,In vivo ,Internal medicine ,medicine ,Animals ,Humans ,General Materials Science ,Myocardial infarction ,3D bioprinting ,Myocardium ,Bioprinting ,General Chemistry ,021001 nanoscience & nanotechnology ,medicine.disease ,In vitro ,0104 chemical sciences ,Disease Models, Animal ,Cardiology ,Stem cell ,0210 nano-technology ,Reperfusion injury ,Ex vivo ,Biotechnology - Abstract
Despite the latest advances in cardiovascular biology and medicine, myocardial infarction (MI) remains one of the major causes of deaths worldwide. While reperfusion of the myocardium is critical to limit the ischemic damage typical of a MI event, it causes detrimental morphological and functional changes known as "reperfusion injury." This complex scenario is poorly represented in currently available models of ischemia/reperfusion injury, leading to a poor translation of findings from the bench to the bedside. However, more recent bioengineered in vitro models of the human heart represent more clinically relevant tools to prevent and treat MI in patients. These include 3D cultures of cardiac cells, the use of patient-derived stem cells, and 3D bioprinting technology. This review aims at highlighting the major features typical of a heart attack while comparing current in vitro, ex vivo, and in vivo models. This information has the potential to further guide in developing novel advanced in vitro cardiac models of ischemia/reperfusion injury. It may pave the way for the generation of advanced pathophysiological cardiac models with the potential to develop personalized therapies.
- Published
- 2020
8. Advanced Cardiac Models: Considerations for the Bioengineering of Advanced Cardiac In Vitro Models of Myocardial Infarction (Small 15/2021)
- Author
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Xiaowei Wang, Andrew J. Boyle, Carmine Gentile, Laura Vettori, Poonam Sharma, Gemma A. Figtree, and Clara Liu Chung Ming
- Subjects
Biomaterials ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Cardiology ,General Materials Science ,General Chemistry ,Myocardial infarction ,medicine.disease ,business ,Biotechnology - Published
- 2021
9. Application of High-Electric-Field Plasma to Medical Fields
- Author
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Liu Chung Ming, Kenichi Iwasaki, Yasushi Nishida, Keng Liang Ou, and Fang Yu Fan
- Subjects
Physics ,Waste management ,Electric field ,General Medicine ,Plasma ,Air cleaning ,Sterilization (microbiology) - Abstract
The high-electric-field plasma (HEFP) phenomenon is reviewed to define its physics and mechanism. Possible applications of the HEFP system in medical and environmental engineering fields are discussed, including sterilization of bacteria, the prolonged preservation of fresh plants, and air cleaning effects.
- Published
- 2013
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