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Additional file 5: Table S4 Summary of primers used in this study.

Additional file 1: Fig. S1. Flowchart of the experimental design and analysis for PacBio sequencing and RNA sequencing. Fig. S2 Read length with different ploidy and genome compositions hybrids. Fig. S3 The number of isoforms of functional annotation of with different ploidy hybrids. Fig. S4 RT-PCR validation of AS events for two genes. Gel bands in each figure show DNA makers and PCR results in five tissues/samples. Fig. S5 The p-value for GO enrichment of isoforms.