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Additional file 2: Supplementary materials and methods.

Additional file 1: Supplementary Figure 1 Normoxic AC16 cells were co-cultured with HR-EMVs at different concentrations (10^7, 10^8 and 10^9 particles/ml) for 24 h: (A) CCK-8 assay for AC16 cell viability. (B) Flow cytometry detection of apoptotic rate in AC16 cells after annexin V-FITC/PI double staining. (C) quantitative analysis of apoptotic cells. HR-injured AC16 cells were co-cultured with HR-EMVs, (HR + P25)-EMVs, (HR + P50)-EMVs, (HR + P100)-EMVs, and (HR + P150)-EMVs for 24 h, respectively: (D) CCK-8 assay for AC16 cell viability. (E) Flow cytometry detection of apoptotic rate in AC16 cells after annexin V-FITC/PI double staining. (F) quantitative analysis of apoptotic cells. These data were representative results (n = 3) of three repetitions. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant. Data are expressed as mean ± SEM. Supplementary Figure 2 (A) HUVECs were infected with LV-sh-lncCCT4 or LV-sh-NC for 72 h. The knockdown efficiency of lncCCT4-2 in HUVECs was detected by RT-qPCR. Transfection of AC16 cells with sh-CCT4 and OE-CCT4 plasmids for 48 h: (B-C) Detection of CCT4 mRNA expression in AC16 cells by RT-qPCR. (D-E) Western blotting analysis of CCT4 protein expression in AC16 cells to detect the transfection efficiency of sh-CCT4 and OE-CCT4 plasmids. These data were representative results (n = 3) of three repetitions. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant. Data are expressed as mean ± SEM. Supplementary 3 The genomic location and sequence of lncCCT4-2 and CCT4. Supplementary 4 Prediction of lncCCT4-2 and CCT4 mRNA binding sites.

Additional file 1. Fig. S1: The effect of miR-132-3p in MSC (passage 2) proliferation and senescence. Cell proliferation of MSC was determined by Cell Counting Kit 8 (CCK-8) assay. The graph shows data coming from MSCCon, MSCmiR-132-3p, and MSCantagomiR-132-3p groups (A). Representative images and summary data showing the senescence of MSC in MSCCon, MSCmiR-132-3p, and MSCantagomiR-132-3p groups. *p＜0.05. (PDF 2433 kb)

In recent years, transmission line icing caused by ice and snow disasters threatens the safe and stable operation of power system. Among the existing ice melting devices, the modular multilevel DC ice melting device has the characteristics of less harmonic content, small volume, simple switching mode, low failure rate and small power capacity, which has significant advantages in economy and technical feasibility. However, because the modular multilevel DC ice melting device is only used during the freezing period in winter, the problem of low utilization rate of the device is also prominent. In order to solve the problems of large volume, high harmonic content, high cost and low utilization rate in the operation of traditional DC ice melting device, this paper proposes a control function and implementation method of modular multilevel DC ice melting device based on SVG. During non-ice melting period, the control strategy can output or absorb reactive power according to the needs of the system to support the grid voltage and improve the utilization rate of equipment. This paper first analyzes the topology and working principle of the modular multilevel DC deicing device, then introduces the carrier phase shift modulation strategy of the modular multilevel DC deicing device, and puts forward the SVG control strategy of the modular multilevel DC deicing device under three-phase balanced and unbalanced conditions, so as to realize load dynamic reactive power compensation and grid voltage drop suppression, The reliability of the system is improved significantly. Finally, based on the above method, the Simulink simulation model is established to verify the effectiveness of the control strategy, and the simulation waveform is given for analysis.

Hardy-Weinberg equilibrium assay for rs653765 and rs514049 genotypes in sepsis patients and healthy controls.