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In this study, the effect of ozonation process on the poly(ADP-ribose) polymerase 1 gene expression (PARP-1) and related the NADPH metabolism in strawberry fruit during storage was determined. Our results showed that ozonation with gas at both 10 and 100 ppm concentrations increased the expression of PARP-1 in the fruit during storage. Furthermore, the ozonation process initially increased the level of NAD

Raspberry fruits are a valuable source of bioactive compounds. The study used the modification of the substrate (coconut fibre), consisting of the use of various organic and mineral additives, in the soilless cultivation of raspberries. The additives influenced the biosynthesis of bioactive compounds in the raspberry fruits by modifying the sorption properties and the abundance of the substrate. The influence of the additives on the content of polyphenols was determined as well as their profile (UPLC-MS), antioxidant potential (ABTS), vitamin C content, and the activity of selected enzymes that are markers of stress and resistance to abiotic factors. In the study, a significant effect of these additives was observed on the biosynthesis of polyphenols in raspberry fruit. The highest increase in the content of these compounds in relation to the control sample (substrate—100% coconut fibre), namely 37.7%, was recorded in the case of fruit produced on coconut substrate enriched with sheep wool. These fruits were also characterised by a significantly different profile of these compounds. These changes were caused by readily available ammonium nitrogen and free amino acids in the decomposition of proteins contained in the sheep wool. This was confirmed by the recorded content of chlorophyll SPAD in the plant leaves and the activity of selected enzymes, which proves a low level of stress and good condition of the plants.

The aim of this study was to evaluate the effect of ozone treatment on the mitochondrial energy metabolism in blueberry fruit during storage as well as to determine the relationship between the activity of mitochondria and the antioxidative properties of ozonated fruit. Blueberry fruit was stored for 28 days at 4 °C and ozonated daily with gaseous ozone at the concentration of 15 mg L−1 for 30 min, every 12 h of storage. Research showed that ozonated fruit was characterized by higher activity of enzymes involved in oxidative phosphorylation (by 58.7% for SDH, 118.2% for CCO, and 78.16% for H+-ATPase after 7 days, respectively) than non-ozonated sample, which contributed to reduction of the loss of energy charge and ATP in the fruit during storage. Moreover, the increased activity of mitochondria led to the growth of mitochondrial ROS accumulation which, in turn, activated defense mechanisms against oxidative stress in the fruit. These metabolic responses might collectively contribute to increase the antioxidative properties of ozonated fruit and consequently to maintain a good quality of the fruit over a long period of storage.

Raspberry fruits are a valuable source of bioactive compounds. The study used the modification of the substrate (coconut fibre), consisting of the use of various organic and mineral additives, in the soilless cultivation of raspberries. The additives influenced the biosynthesis of bioactive compounds in the raspberry fruits by modifying the sorption properties and the abundance of the substrate. The influence of the additives on the content of polyphenols was determined as well as their profile (UPLC-MS), antioxidant potential (ABTS), vitamin C content, and the activity of selected enzymes that are markers of stress and resistance to abiotic factors. In the study, a significant effect of these additives was observed on the biosynthesis of polyphenols in raspberry fruit. The highest increase in the content of these compounds in relation to the control sample (substrate-100% coconut fibre), namely 37.7%, was recorded in the case of fruit produced on coconut substrate enriched with sheep wool. These fruits were also characterised by a significantly different profile of these compounds. These changes were caused by readily available ammonium nitrogen and free amino acids in the decomposition of proteins contained in the sheep wool. This was confirmed by the recorded content of chlorophyll SPAD in the plant leaves and the activity of selected enzymes, which proves a low level of stress and good condition of the plants.

Fruits of Amelanchier alnifolia Nutt. ex M. Roem. (Nutt.) are a good source of bioactive compounds and vitamins. Due to the fact that the berries are a soft fruit, they require special procedures to increase their molecular and mechanical stability during cold storage. The study investigated the effects of ozone treatment applied cyclically (every 24 h) on selected chemical and mechanical parameters of saskatoon berries kept in storage. For this purpose, measurements were performed to assess changes in some molecular markers such as antioxidant potential, content of vitamin C, and total polyphenols, as well as microbial stress and maximum destructive force under uniaxial compression of samples. The effectiveness of the storage process was also assessed in relation to the conditions used by determining the proportion of fruit affected by diseases occurring in storage. The findings show that ozone treatment led to increased content of bioactive compounds at the initial stages of storage and resulted in decreased loss of water and bioactive compounds at the later stages. Ultimately, irrespective of the conditions applied during ozone treatment, it was observed that the growth of micro-organisms on the fruit surface was inhibited, and as a result, storage losses during the relevant period were significantly reduced.

Fruits of

Research into the suitability of domestic raw materials, including, for example, new wheat cultivars and fruit additives for the production of flavoured beers, is increasingly being undertaken by minibreweries and craft breweries. The fruits of the Saskatoon berry are an important source of bioactive compounds, mainly polyphenols, but also macro- and microelements. The fruits of two Canadian cultivars of this species, ‘Honeywood’ and ‘Thiessen’, were used in this study. Physicochemical analysis showed that wheat beers with the addition of non-ozonated fruit were characterised by a higher ethanol content by 7.73% on average. On the other hand, enrichment of the beer product with fruit pulp obtained from the cv. ‘Thiessen’ had a positive effect on the degree of real attenuation and the polyphenol profile. Sensory evaluation of the beer product showed that wheat beers with the addition of ‘Honeywood’ fruit were characterised by the most balanced taste and aroma. On the basis of the conducted research, it can be concluded that fruits of both cvs. ‘Honeywood’ and ‘Thiessen’ can be used in the production of wheat beers, but the fermentation process has to be modified in order to obtain a higher yield of the fruit beer product.

Research into the suitability of domestic raw materials, including, for example, new wheat cultivars and fruit additives for the production of flavoured beers, is increasingly being undertaken by minibreweries and craft breweries. The fruits of the Saskatoon berry are an important source of bioactive compounds, mainly polyphenols, but also macro- and microelements. The fruits of two Canadian cultivars of this species, 'Honeywood' and 'Thiessen', were used in this study. Physicochemical analysis showed that wheat beers with the addition of non-ozonated fruit were characterised by a higher ethanol content by 7.73% on average. On the other hand, enrichment of the beer product with fruit pulp obtained from the cv. 'Thiessen' had a positive effect on the degree of real attenuation and the polyphenol profile. Sensory evaluation of the beer product showed that wheat beers with the addition of 'Honeywood' fruit were characterised by the most balanced taste and aroma. On the basis of the conducted research, it can be concluded that fruits of both cvs. 'Honeywood' and 'Thiessen' can be used in the production of wheat beers, but the fermentation process has to be modified in order to obtain a higher yield of the fruit beer product.

The major aim of this study was to check the effect of one-time ozonation on selected quality parameters and antioxidant status of Actinidia arguta fruit. For this purpose, A. arguta fruit was ozonated with gas at a concentration of 10 and 100 ppm, which was carried out successively for 5, 15 and 30 min. Next, the selected quality attributes, antioxidants level as well as NADPH and mitochondrial energy metabolism in mini-kiwi fruit after ozonation were analysed. Our research has shown that ozonation reduced the level of yeast and mould without affecting the content of soluble solids or acidity. In turn, ozonation clearly influenced the antioxidant activity and the redox status of the fruit. The ozonated fruit was characterised by a lower level of ROS due to the higher level of low molecular weight antioxidants, as well as the higher activity of superoxide dismutase and catalase. In addition, improved quality and antioxidant activity of the fruit were indirectly due to improved energy metabolism and NADPH level. The ozonated fruit showed a higher level of ATP, due to both higher activity of succinate dehydrogenase and higher availability of NADH. Moreover, the increased level of NAD

This work aimed to detect changes in trace element chemical speciation in sewage sludge (SS) after the ozonation process. The modified Community Bureau of Reference (BCR) sequential extraction procedure was performed to determine the chemical speciation of trace elements in SS after the ozonation process. To assess potential soil contamination with trace elements from sewage sludge after the ozonation process, the risk assessment code (RAC) coefficient was used. The bioaccumulation factor (BAF) and translocation factor (TF) values were also calculated to characterize the efficiency of trace element accumulation in the studied plant species from soil fertilized with sludges after the ozonation process. Generally, the mean concentration of total trace elements in the SS after the ozonation process was higher, but the differences were statistically significant only in the case of Mn, Cu, Pb, and Cd. The dominant fraction of Fe, Cr, Pb, and Cd was the residual fraction F4, while the extractable/exchangeable fraction F1 was present in the smallest amount. Therefore, in the case of Mn, Zn, and Ni, the ozonation process had a significant impact on the increase in the content of these elements in the F1 fraction. The application of the SS stabilized by ozonation process for maize and wheat fertilization did not significantly affect the bioaccumulation of most of the analyzed metals in aboveground biomass. Higher values of BAF coefficients after the application of ozonated SS were found only in the case of Cu and Ni. In turn, the determined TF coefficients were lower than 1 in most cases. The obtained results showed that the slight change in the concentration of Zn, Mn, and Ni in fraction F1 causes a specific risk of their mobility in the soil environment. It should be noted that due to the variable composition of sewage sludge, an analysis of the content of individual trace elements in chemical fractions should be carried out to assess its actual impact on the environment. This can help to indicate further actions that should be undertaken to limit their negative impact on the environment.

The major aim of this research was to investigate the effect of ozone treatment on the energy metabolism in raspberry fruit during storage at room temperature. Raspberries were ozonated with an ozone concentration of 8–10 mg L−1, for 30 min, every 12 h of storage at room temperature for 72 h. The results indicated that ozone treatment significantly enhanced the activities of mitochondrial respiratory enzymes, such as succinate dehydrogenase, cytochrome C oxidase, and H+-ATPase, which contributed to maintain the high level of ATP and energy charge in fruit during storage. Moreover, the energy metabolism in mitochondria was closely correlated with the antioxidant potential of raspberry fruit. This study has given an experimental evidence that ozonation procedure in proposed process conditions significantly affects the mitochondrial respiratory system leading to maintain the high quality of the fruit over a long period of storage at room temperature.

This study examined the impact of the ozonation procedure on the content of bioactive compounds in young shoots of Pinus sylvestris L. as well as on the yield and the composition of the essential oils and volatile fraction. The ozonation process consisted of exposure of the raw material during 5, 15 and 30 min to the ozone gas with the concentration of 10 and 100 ppm, and then it was stored for 24 hours. In the ozonated pine shoots, the content of vitamin C overall content of polyphenol content and antioxidant potential were determined. It was found that the investigated parameters increased as a result of the utilized procedure. The observed increase in the content of tested bioactive compounds was dependent on exposition time and was found to be highest for ozonated material for 15 min with ozone gas at a concentration of 10 ppm. As a result of the ozonation procedure the content of polyphenolic compounds and vitamin C content increased. Whereas it, did not have a significant effect on composition of the obtained essential oils. It was found that ozone is an effective elicitor, which allows increasing the content of bioactive compounds in pine shoots.

Herbs can be contaminated with microorganisms, which affects their quality while increasing the risk of food poisoning. Thus, there is a need for efficient decontamination techniques. In this study, the microbial load was reduced in peppermint herb (Mentha piperita L.) exposed to ozone at 10 ppm for 60 minutes. The quality of the ozonated herb was assessed by the determination of microbial load and essential oils content. Total microbial content was reduced by 4 log cfu g−1 of the microorganisms whereas the contamination with molds was reduced from 10.07 log cfu g−1 of the microorganisms to zero. Moreover, sensory evaluation of the herb aroma was conducted. These characteristics were not significantly affected by the treatment. Therefore, ozone can be used to improve the peppermint herb quality by reduction of the microbial contamination of peppermint herb while having no adverse effect on the herb aroma.

The major aim of this study was to check whether a cyclic ozonation process will affect the preservation of the texture of strawberries stored at room temperature. Strawberry fruit was stored for 3 days at room temperature and ozonated with gaseous ozone at a concentration of 10 and 100 ppm for 30 min, every 12 h of storage. Research showed that the ozonation process inhibited the texture deterioration of the fruit during storage. The positive effect of ozone was directly related to the inhibition of the activity of enzymes involved in the degradation of the fruit cell walls, as well as indirectly from the improved energy metabolism of the fruit. The higher level of energy charge corresponded to the higher resistance of ozonated fruit to abiotic stress, leading to the maintenance of the integrity of cell membranes and, consequently, to maintaining good hardness of the fruit throughout the storage period.

The study concerns the effects of drying process conditions (40, 50, 60°C) on the contents of bioactive compounds in Scots pine (Pinus sylvestris L.) shoots as well as the yield and composition of essential oils. The obtained dry material was examined for the total contents of polyphenols and the antioxidant activity as well as the content of vitamin C. The material was examined by using the HS-SPME method, and the composition of the essential oils was determined using gas chromatography method (GC-MS). The highest contents of bioactive compounds were found in the material subjected to drying at 40°C. The highest yield of essential oils (0.63 ml 100 g−1 d.m. of the material) was obtained from the pine shoots dried at 40°C.

The major aim of this study was to investigate the effect of ozonation process on the level of oxidative stress markers in blueberry fruit during cold storage (4 °C). Blueberry (Vaccinum corymbosum L.) fruit was ozonated with an ozone concentration of 15 ppm for 30 min, every 12 h for 28 days of storage at 4 °C. The results indicated that ozone treatment activated a defense mechanism against oxidative stress in blueberry fruit. Ozonated fruit was characterized by higher activity of antioxidant enzymes i.e. superoxide dismutase, glutathione peroxidase, and phenylalanine ammonia-lyase than non-ozonated fruit, over the first 21 days of storage. In turn, the level of superoxide anion radical and hydrogen peroxide in ozonated fruit was significantly lower compared with the untreated material. However, after 21 days of storage, ozone treatment contributed to the oxidative modification of protein which could be a reason of decreasing enzymes activity, involved in cell protection against oxidative stress.

Salt stress is one of the main stressors limiting plant growth and yield. As a result of salt stress, unfavorable changes in the photosynthesis process take place, leading to a decrease in plant productivity. Therefore, it is necessary to use biologically active substances that reduce the effects of this stress. An example of such a substance is quercetin, classified as a flavonoid, which plays an important role in alleviating the effects of salt stress, mainly by the inactivation of reactive oxygen species (ROS) and by improvement of the photosynthesis process. A study was made of the effect of the quercetin-copper complex (Q-Cu (II)), which has a stronger antioxidant effect than pure quercetin. By means of a pot experiment, the influence of solutions of the Q-Cu (II) complex (100 mg∙L

Celem prezentowanych badań było określenie wpływu procesu ozonowania na poziom związków polifenolowych oraz aktywność wybranych enzymów zaangażowanych w metabolizm polifenoli w owocach borówki wysokiej (Vaccinum corymbosum L.) podczas przechowywania. Materiał badawczy stanowiły owoce borówki wysokiej odmiany Bluecrop, które były ozonowane gazem o stężeniu 15 ppm, przez 30 min, co 12 godzin przechowywania w temperaturze 4°C przez 28 dni. Owoce były poddawane analizie zawartości związków polifenolowych, aktywności antyoksydacyjnej oraz aktywności enzymów uczestniczących w ich biosyntezie oraz rozkładzie. Wyniki pokazały, że proces ozonowania aktywuje szlak biosyntezy polifenoli w owocach borówki wysokiej. W owocach ozonowanych stwierdzono wyższą aktywność hydroksylazy 3ß-flawononu oraz syntazy flawonolu niż w owocach nieozonowanych, co korespondowało z wyższą zawartością większości analizowanych polifenoli i aktywnością antyoksydacyjną. Ponadto, po 2 tygodniu przechowywania stwierdzono niższą aktywność oksydazy polifenolowej w owocach ozonowanych. Powyższa odpowiedź metaboliczna owoców przyczyniła się do większej odporności owoców na stres abiotyczny, jak również do utrzymania wysokiej jakości owoców, w tym wartości biologicznej podczas przechowywania. Powyższe wyniki badań zostały zaprezentowane na Ogólnopolskiej Konferencji Naukowej pt. „Człowiek - Żywność -Środowisko” dnia 15 października 2021 r. w Rzeszowie.

Paenibacillus larvae is a deadly pathogen for bee brood, which can lead to the death of entire colonies. The presence of specific volatile organic compounds (VOCs) in the hive may be related to the occurrence of this bacterium in brood. Compositions of those volatile fractions present in healthy brood from control colonies and the brood without symptoms of infection collected from the colonies infected by P. larvae were compared using gas chromatography coupled with mass spectrometry (GC-MS) and solid phase microextraction (SPME). Among the seven compounds detected and quantified, the relative concentrations of 3-carene and limonene significantly differentiated the brood from healthy and infected colonies. Based on the ratio analysis, the samples were differentiated in terms of the number of emitted VOCs.

Elderflowers are a well-known source of bioactive compounds. The amount of isolated bioactive compounds may be increased by applying various abiotic and biotic factors. Gaseous ozone (10 and 100 ppm) was used in the process of preparing flowers. Next, the flowers were treated with sugar syrup to extract bioactive compounds. It was shown that this treatment, including the influence of extraction temperature, significantly affects the contents of polyphenols (liquid chromatography–mass spectrometry (LC–MS) methods) and vitamin C, as well as the antioxidant potential (cupric reducing antioxidant capacity (CUPRAC method)), the profile of volatile substances (head space–solid-phase microextraction (HS–SPME methods)) and the colour of the syrup (Commission Internationale de l’Eclairage (CIE) L*a*b* methods). The findings show that an increased dose of ozone and higher extraction temperature applied in the process of syrup production resulted in higher contents and different compositions of bioactive compounds. The highest contents of bioactive compounds were identified in syrup obtained from raw material treated with ozone for 15 min (concentration = 10 ppm) and extraction with sugar syrup at a temperature of 60 °C.

Young barley plants are a good source of bioactive compounds. This paper presents the effects of gaseous O3 (trioxygen or ozone) on the biosynthesis of compounds, determining the antioxidant potential of young barley plants. The total content of polyphenols was determined along with their profile, as well as total antioxidant potential and vitamin C content. The highest contents of these compounds were identified in young barley plants exposed to gaseous O3. The main bioactive compound, representing polyphenols, determined in the examined raw materials was saponarin (isovitexin 7-O-glucoside). The induction of increased biosynthesis of these molecules was directly linked to the modification of the activity of selected enzymes. The increased polyphenol content resulted from the modified activities of polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL). On the other hand, the oxidative effect of ozone on barley plants was reduced, owing to the modified activities of catalases (CAT), glutathione peroxidases (SOD) and guaiacol peroxidase (GPOX). Analysis of the results showed that by applying gaseous O3 at a dose of 50 ppm for 10 min, the contents of bioactive compounds can be maximised in a residue-free way by activating oxidative stress defence mechanisms.

Elderflowers are a well-known source of bioactive compounds. The amount of isolated bioactive compounds may be increased by applying various abiotic and biotic factors. Gaseous ozone (10 and 100 ppm) was used in the process of preparing flowers. Next, the flowers were treated with sugar syrup to extract bioactive compounds. It was shown that this treatment, including the influence of extraction temperature, significantly affects the contents of polyphenols (liquid chromatography–mass spectrometry (LC–MS) methods) and vitamin C, as well as the antioxidant potential (cupric reducing antioxidant capacity (CUPRAC method)), the profile of volatile substances (head space–solid-phase microextraction (HS–SPME methods)) and the colour of the syrup (Commission Internationale de l’Eclairage (CIE) L*a*b* methods). The findings show that an increased dose of ozone and higher extraction temperature applied in the process of syrup production resulted in higher contents and different compositions of bioactive compounds. The highest contents of bioactive compounds were identified in syrup obtained from raw material treated with ozone for 15 min (concentration = 10 ppm) and extraction with sugar syrup at a temperature of 60 °C.

Plant production technologies based solely on the improvement of plants themselves face obstacles resulting from the natural limitations of the biological potential of varieties. Therefore, new substances are sought that positively influence the growth and development of plants and increase resistance to various biotic and abiotic stresses, which also translates into an increase in obtained yields. The exogenous application of various phytoprotectants shows great promise in terms of cost effectiveness compared to traditional breeding methods or transgenic approaches in relation to increasing plant tolerance to abiotic stresses. Quercetin is a strong antioxidant among phenolic compounds, and it plays a physiological and biochemical role in plants. As such, the aim of this research was to assess the effect of an aqueous solution of a quercetin derivative with potassium, applied in various concentrations (0.5%, 1.0%, 3.0% and 5.0%), on the efficiency of the photosynthetic apparatus and biochemical properties of maize. Among the tested variants, compared to the control, the most stimulating effect on the course of physiological processes (PN, gs, ci, CCI, Fv/Fm, Fv/F0, PI) in maize leaves was found in 3.0 and 5.0% aqueous solutions of the quercetin derivative. The highest total antioxidant capacity and total content of polyphenolic compounds were found for plants sprayed with 5.0% quercetin derivative solution, therefore, in this study, the optimal concentration could not be clearly selected.

Quercetin, classified as a flavonoid, is a strong antioxidant that plays a significant role in the regulation of physiological processes in plants, which is particularly important in the case of biotic and abiotic stresses. The study investigated the effect of the use of potassium quercetin solutions in various concentrations (0.5%, 1.0%, 3.0% and 5.0%) on the physiological and biochemical properties of wheat seedlings. A pot experiment was carried out in order to determine the most beneficial dose of this flavonoid acting as a bio-stimulant for wheat plants. Spraying with quercetin derivative solutions was performed twice, and physiological measurements (chlorophyll content and fluorescence as well as gas exchange) were carried out on the first and seventh days after each application. The total phenolic compounds content and the total antioxidant capacity were also determined. It was shown that the concentrations of potassium quercetin applied have a stimulating effect on the course of physiological processes. In the case of most of the tested physiological parameters (chlorophyll content and fluorescence and gas exchange) and the total antioxidant capacity, no significant differences were observed in their increase as a result of application with concentrations of 3.0 and 5.0%. Therefore, the beneficial effect of quercetin on the analysed parameters is already observed when spraying with a concentration of 3.0%.

Among the waste produced by municipal wastewater treatment plants, the largest technological problem cause the excessive sludge, and its management consumes almost half of the costs of the entire installation. Regardless of the final disposal route, which may be, for example, reclamation of degraded areas, wastewater plant derived sludge should be firstly subjected to a series of processes aimed at: reducing its volume, improving selected physical properties, energy recovery, rot preventing (stabilization) or elimination of epidemiological threats (hygenizing). These goals could be implemented using commonly known technologies, including: dewatering, liming, thermoconditioning, methanogenesis or composting. One of the alternative solutions is the use of ozonation of raw excess sludge, which, in principle, may lead to the simultaneous resolution of several above mentioned problems as: drainage properties improvement, increase the bioavailability of organic matter for biogas production, as well as the removal of undesirable microorganisms. Confirmation of the hygenizing effects usually requires arduous microbiological methods, that's the fast and reliable tools of molecular biology could prove its usefulness in this case. In this study, the possibility of determining the dynamics of selected groups of microorganisms (including changes in total bacteria amount, Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria) after sewage sludge ozonation, by using the semi-quantitative RealTime polymerase chain reaction (qPCR), has been investigated.

The crystallization kinetics was determined for mixtures of stereoisomers which revealed solid–solution phase behavior in the crystalline phase. Two model systems of pharmaceutically active compoun...

The aim of this research was to show the effect of the ozonation process on the quality of sea buckthorn (Hippophae rhamnoides L.). The quality of the ozonated berries of sea buckthorn was assessed. Prior to and after the ozone treatment, a number of parameters, including the mechanical properties, moisture content, microbial load, content of bioactive compounds, and composition of volatile compounds, were determined. The influence of the ozonation process on the composition of volatile compounds and mechanical properties was demonstrated. The ozonation had negligible impact on the weight and moisture of the samples immediately following the treatment. Significant differences in water content were recorded after 7 days of storage. It was shown that the highest dose of ozone (concentration and process time) amounting to 100 ppm for 30 min significantly reduced the water loss. The microbiological analyses showed the effect of ozone on the total count of aerobic bacteria, yeast, and mold. The applied process conditions resulted in the reduction of the number of aerobic bacteria colonies by 3 log cfu g−1 compared to the control (non-ozonated) sample, whereas the number of yeast and mold colonies decreased by 1 log cfu g−1 after the application of 100 ppm ozone gas for 30 min. As a consequence, ozone treatment enhanced the plant quality and extended plant’s storage life.

In this study, we investigated the effects of the utilization of seed coating by dedicated fertilizer on the mechanism of oxidative stress reduction in maize growing in simulated drought conditions. A strict pot experiment was conducted for this purpose in a climatic chamber with a phytotron system and controlled temperature and air humidity. Maize seeds were planted and grown in soil with 20% (extreme drought), 40%, and 60% water holding capacity (WHC). The seeds were enhanced using proposed fertilizer and applied at a rate of 2 kg t−1 seeds. The levels of ROS, as well as antioxidant enzymes SOD, CAT, and GPOX, generated by plants enhanced using the seed coating by fertilizer and by control specimens (non-enhanced), were measured 21 days after the seeds were planted. Antioxidant potential and total polyphenol contents in the plants were also determined. The findings show that under drought stress, plants produce high levels of ROS, which is responsible for oxidative stress. However, the latter phenomenon may be reduced using seed coating. Application of seed coating by fertilizer contributed to a 32.7% decrease in ROS in the case of extreme drought (soil with 20% WHC). The treatment also led to increased activity of SOD (61.2%), CAT (45.7%), and GPOX (35.8%), which shows its positive effects on activation of the enzymatic antioxidant system responsible for neutralization of ROS and for reducing the negative effects of drought.

Salt stress is one of the main stressors limiting plant growth and yield. As a result of salt stress, unfavorable changes in the photosynthesis process take place, leading to a decrease in plant productivity. Therefore, it is necessary to use biologically active substances that reduce the effects of this stress. An example of such a substance is quercetin, classified as a flavonoid, which plays an important role in alleviating the effects of salt stress, mainly by the inactivation of reactive oxygen species (ROS) and by improvement of the photosynthesis process. A study was made of the effect of the quercetin–copper complex (Q-Cu (II)), which has a stronger antioxidant effect than pure quercetin. By means of a pot experiment, the influence of solutions of the Q-Cu (II) complex (100 mg∙L−1 [Q1], 500 mg∙L−1 [Q2] and 1000 mg∙L−1 [Q3]) on the physiological and biochemical processes occurring in wheat plants subjected to salt stress was investigated. The plants were given two sprays of Q-Cu (II) solution, and their physiological parameters were examined both 1 and 7 days after each application of this solution. The level of ROS and the activity of antioxidant enzymes (catalase [CAT], superoxide dismutase [SOD] and guaiacol peroxidase [GPOX]) were also determined. It has been shown that spraying with Q2 and Q3 solutions improves the chlorophyll content, the values of chlorophyll fluorescence parameters (the photochemical efficiency of PS II [Fv/Fm], the maximum quantum yield of primary photochemistry [Fv/F0], and the performance index of PS II [PI]), and gas exchange (net photosynthetic rate [Pn], stomatal conductance [gs], transpiration rate [E] and intercellular CO2 concentration [Ci]). As a result of the application of Q2 and Q3 solutions, the level of ROS and the activity of the antioxidant enzymes tested decreased, which means that these concentrations are most effective in counteracting the effects of salt stress.

This study presents the effects of ozone treatment on microbial status and contents of selected bioactive compounds in marjoram plants. Origanum majorana L. is a widely used plant which in the course of production is affected by microbial infections. One of the ways to reduce microbial load involves application of a strong oxidant, such as ozone. In order to determine the effects of ozonation, a number of analyses were carried out including microbiological tests (aerobic colony count, yeast and mould count, and mesophilic lactic acid bacteria count) and chemical tests assessing total antioxidant potential, total polyphenols, and volatile fraction composition. Ultimately, the findings showed considerable (6-log) reduction in microbial load, with unchanged composition of headspace volatile compounds. Furthermore, the raw material obtained presented elevated the contents of the selected bioactive compounds. It was shown that the most beneficial effects are achieved when ozone treatment is applied at a rate of 1 ppm for a duration of 10 min.

This study presents the effects of ozone treatment on microbial status and contents of selected bioactive compounds in marjoram plants. Origanum majorana L. is a widely used plant which in the course of production is affected by microbial infections. One of the ways to reduce microbial load involves application of a strong oxidant, such as ozone. In order to determine the effects of ozonation, a number of analyses were carried out including microbiological tests (aerobic colony count, yeast and mould count, and mesophilic lactic acid bacteria count) and chemical tests assessing total antioxidant potential, total polyphenols, and volatile fraction composition. Ultimately, the findings showed considerable (6-log) reduction in microbial load, with unchanged composition of headspace volatile compounds. Furthermore, the raw material obtained presented elevated the contents of the selected bioactive compounds. It was shown that the most beneficial effects are achieved when ozone treatment is applied at a rate of 1 ppm for a duration of 10 min.

The aim of the study was to identify the effects of ozone treatment reflected by the microbial, mechanical properties and selected chemical parameters during the storage of rhubarb petioles. For this purpose, after they were harvested, rhubarb petioles were treated with gaseous ozone at concentrations of 10 and 100 ppm, for the duration of 5, 15 and 30 min. Subsequently, the plant material was stored at room temperature for 14 days. After this time, the raw material was subjected to a number of chemical and mechanical tests. It was shown that the rhubarb petioles treated with ozone at a rate of 100 ppm for 30 min were characterized by the lowest loss of water content. It was also found that, compared to the control, most samples subjected to ozone treatment presented better mechanical properties, as well as higher oxidative potential and contents of polyphenols and vitamin C. Based on these findings, it was determined that ozone treatment largely increases storage stability of rhubarb.

This study investigates the effects of various drying methods applied to leaves of Cistus creticus L. on the contents of polyphenols and the composition of the volatile fraction. The following four drying methods were used: convection drying at a temperature of 40 °C (CD 40 °C), 50 °C (CD 50 °C), and 60 °C (CD 60 °C); vacuum-microwave (VMD 240 W); combined drying, involving convection pre-drying (50 °C) and vacuum-microwave (240 W) finish drying (CPD-VMFD) as well as freeze-drying (FD). Polyphenols in the dried leaves were determined using chromatography-photodiode detector-quadrupole/time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS). The contents of odoriferous substances in the dry material were determined by means of head space-solid phase microextraction (HS-SPME) with the use of a gas chromatograph (GC). Thirty-seven polyphenol components including 21 flavonols, eight flavan-3-ols, and eight hydrolyzed tannins in dry Pink Rock Rose material were found for the first time. The highest contents of polyphenols, totaling 2.8 g 100 g−1 dry matter (d.m.), were found in the samples subjected to the CPD/VMFD drying method. Pink Rock Rose subjected to this drying method was characterized by large quantities of odoriferous compounds, mainly eugenol, thymol, and carvacrol, which contribute to its antiseptic properties. By using CPD/VMFD methods, it is possible to obtain fine quality dry material from the leaves of C. creticus.

This manuscript presents the effect of foliar and soil fertilizer produced from thermally processed bone waste on the initial growth and development of maize plants. The developed fertilizers were tested in three different doses in a pot experiment. Because nutrient deficiency interferes with plant physiological processes, the impact of the developed fertilizers on gas exchange parameters, relative chlorophyll content, and chlorophyll fluorescence parameters were assessed. Based on the conducted research, it was found that fertilization with developed foliar and soil fertilizers increased the relative content of chlorophyll in maize leaves and increased the value of gas exchange parameters and chlorophyll fluorescence. All determined parameters of gas exchange and chlorophyll fluorescence showed a positive correlation depending on the dose of foliar fertilization used (average value r = 0.8414). In turn, the soil fertilization that was utilized during the experiment significantly correlated only with the content of chlorophyll (r = 0.6965). The tested fertilizers improvement of the physiological parameters of the plants, which indicates the fertilizing efficiency of the tested fertilizers.

Background A microgranule fertilizer was designed for localized fertilization of soil with controlled release of nutrients. The microgranule matrix was fortified with proteins, which were obtained from food industry byproducts or waste, i.e., whey protein from milk serum, soy protein from soy isolate and egg white protein from chicken egg white powder. The mechanism of the protein decomposition and migration of micro and macromolecule compounds through two different model soil systems was investigated. The potential of the protein fortified fertilizer for localized fertilization of the potted maize seeds was evaluated. Results The study revealed that proteins slowly diffused through soil with simultaneous degradation, which was accompanied with release of ammonia ions. The highest concentration of proteins and degradation products was found in a close vicinity of the microgranule. The microgranules were used as a local fertilizer for maize seeds in the pot experiments. The experiments confirmed statistically significant improvement in root density of maize plant compared to control group. Conclusions Byproducts or waste of food industry, such as the milk serum and soy can be used as a source of proteins that degrade in soil without a pretreatment. The degradation is accompanied with formation of ammonium ions, which can be utilized by plants as a nitrogen source. The fertilizer microgranule should be placed in a close vicinity to the plant seed, since the maximum of the protein concentration and ammonia ions is reached at a very close distance from the microgranule.

Due to a variety of physicochemical properties of the sewage sludge the selection of proper methods for its stabilization is difficult. In this work, the effect of ozone treatment on physico-chemical and microbiological parameters of sludge was investigated. Ozonation was carried out using a counter-current bed reactor, which ensures optimal mass transfer and the contact time of ozone with excessive sediment. The ozonation process was carried out for 15, 30, 45 and 60 min at an ozone concentration of 90−110 ppm. As a result of the ozone treatment it was possible to reduce the number of colony forming units. Also the potential for dewatering of the sludge improved while the total solids of the sludge did not change. The values of COD and BOD5 increased with time of the process.

In this research the impact of ozonation process of wheat flour in fluidized state on the activity of enzymatic apparatus was investigated. Experimental design were performed by means of a Box-Behnken design matrix, using a two factors and three levels of factors (ozone concentration: 5, 15, 30 ppm, and time of process: 5, 15, 30 min). Biochemical analyzes included determination of total amylolytic, proteolytic, lipolytic, and lipoxygenase activity depending on the ozonation process conditions. It was found that activity of ozonated flour enzymes depended on the ozone concentration in the fluidized bed reactor and time of process. The total activity of amylases, proteases and lipases showed a downward trend, with an increase in the ozone concentration in the reactor and duration of the process. In turn, the activity of lipoxygenase increased significantly. Ozonation of wheat flour in fluidized state could be useful tool to control enzymatic activity of wheat flour.

In the studies conducted, the impact of the innovative ozonation procedure on the microbial state and antioxidant potential of highbush blueberry (Vaccinum corymbosum L.) stored under cold storage ...

Ground cucumber, as a nutrient, is a product highly valued by consumers in both fresh and processed form. The aim of the research was to develop a technology for extending the shelf life of...

The potential of the ozone-enriched atmosphere for the improvement of the shelf life of apples of Gloster variety (Malus domestica) in cold storage was investigated. The storage experiment was cond...

The major aim of this study was to evaluate the usefulness of the flavonoid – phosphatidylcholine coating in inhibiting the lipid peroxidation process in flaxseed oil during storage. The level of malondialdehyde, fatty acid profile and antioxidant activity of flaxseed oil stored in a vessel covered with onion skin extract – phosphatidylcholine coating for 7 weeks at room temperature were analyzed. The application of a flavonoid-coating on the walls of a vessel impeded adverse changes caused by the process of lipid peroxidation while storing flaxseed oil. The level of malondialdehyde was significantly lower in oil stored with antioxidant coating than control sample during whole period of storage. Moreover, antioxidants released from onion skin extract reduced a degree of fatty acid modification in oil as well as increased the biological activity of flaxseed oil.

Cucumber souring is a process mediated by a microorganisms present on the surface of cucumber fruits. Sour pickles are fresh cucumbers that have been poured over with a sodium chloride solution and various types of spices. In the present research, pre-ozonated and control cucumber fruits were subjected to the souring processes. It was observed that ozone treatment had no negative impact on the quality of the fruit before the souring process. Moreover, sour pickles after pre-ozonation had better mechanic and sensory properties than the control.