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Fibroblasts are key orchestrators of inflammation. Little is known whether these cells change phenotype during resolution of inflammation. We adopted a method to visualise fibroblast activation during inflammation in humans in vivo, which is based on a fibroblast activation protein (FAP) tracer detected by positron emission tomography (PET). While tracer accumulation was high in active arthritis, it decreased significantly after TNF- and IL-17A inhibition. Biopsy-based scRNA-seq analyses in experimental arthritis demonstrated that FAP signal reduction reflected a phenotypic switch from pro-inflammatory MMP3+/IL6+ fibroblasts (high FAP internalisation) to pro-resolving CD200+DKK3+ fibroblasts (low FAP internalisation). Spatial transcriptomics of human joints revealed that pro-resolving niches of CD200+DKK3+ fibroblasts clustered with innate lymphoid cells (ILC)2, whereas MMP3+/IL6+ fibroblasts were co-localised with inflammatory immune cells. CD200+DKK3+ fibroblasts stabilised the ILC2 phenotype and induced resolution of arthritis via CD200/CD200R1 pathway. Taken together, these data suggest a dynamic molecular regulation of the mesenchymal compartment during resolution of inflammation.

In this contribution, new data concerning Algae, bryophytes, fungi and lichens of the Italian flora are presented. It includes new records and confirmations for the algal genus Nitella, for the bryophyte genera Anthoceros, Dicranodontium, Fontinalis, and Riccia, the fungal genera Inocybe and Xerophorus, and the lichen genera Bagliettoa, Biatora, Calicium, Cladonia, Coniocarpon, Lecanora, Opegrapha, Placynthium, Rhizocarpon, Scytinium, Solenopsora, Stereocaulon, and Verrucaria.

Industrial symbiosis is an eco-innovative system concept that is based on a circular economy and industrial ecology ideas. This process comprises the movement of materials, energy, and skills across enterprises located within eco-industrial parks, and strives to provide economic, environmental, and social competitive advantages for all the involved parties. Considering that the agri-food system creates a vast quantity of by-products along the supply chain, it is a sector that has huge potential within material and energy recovery systems and fits well into eco-industrial parks. The current study is a literature review that aims to evaluate the interest exhibited so far by scientific research in the topic of industrial symbiosis in the agri-food sector and to highlight the primary analytical techniques that have been used for this topic. Using the paradigm of multiple correspondence analysis, a content analysis was conducted from which the major themes of the researched phenomena emerged. The results indicate that the topic areas are unrelated and somewhat distant from each other. The analyzed case studies have revealed that the authors had neglected the communicative and collaborative elements among stakeholders, and instead focused on the potential use of some tools and approaches. Furthermore, it has been hypothesized that the hiding of information within a supply chain prevents industrial symbiosis procedures from being implemented. This research suggests the necessity of creating communication and cooperation platforms among stakeholders, which would promote the introduction of new techniques and tools for the development of circular production systems.

Circular bioeconomy represents a recent political vision expected to contribute in tackling the main challenges faced when sustainable industrial transition trajectories are to be implemented. Coordination and interdependence among actors are crucial steps for value creation when developing new sustainable supply chains. Current study is based on a choice experiment devised with contract design theory applied to a specific supply chain in which a circular bioeconomy strategy is implemented. It investigates the propensity of Sicilian millers to participate in a novel supply chain in which feedstuff is produced by processing a by-product, namely olive cake. Furthermore, millers contract attributes’ preferences are analysed. The results from two econometric models reveal that 71% of the interviewed entrepreneurs would participate in the proposed supply chain while the propensity to participate is positively related with firm size and millers’ attitudes but decreases if millers experienced previous investments or have previously participated in cooperatives. Moreover, respondents prefer shorter length of contract, with a minimum guarantee price, with a renegotiation option, and without the obligation of a minimum volume of product to be supplied. Contract agreements, when contract characteristics are designed ad hoc, are proved to be effective tools for circular bioeconomy supply-chain development.

This study aims to examine Italian consumer preferences for extra virgin olive oil (EVOO) enriched with vitamins and to analyze the key drivers that affect consumer choices for this product. Specifically, we assessed consumers’ intention to purchase the enriched product compared to the conventional one. The methodology adopted inferential and multivariate statistical techniques: (1) exploratory factor analysis (EFA), (2) ordinary least squares regression (OLS) and (3) non-hierarchical clustering. This study appears to be the first research project related to exploring consumers’ interest in an extra virgin olive oil enhanced with vitamins, thereby providing preliminary indications. The main results represent a significant starting point for the development of new marketing strategies for the food industry.

As part of a project aimed at investigating the specific and intraspecific taxa of Matthiola which grow spontaneously in Sicily (Italy), in this work our research has been focused on Matthiola tricuspidata and Matthiola sinuata. The phenolic and volatile compounds of the hydroalcoholic extracts from the aerial parts of the selected species have been characterized by HPLC-PDA/ESI-MS and SPME-GC/MS analyses. Moreover, the antioxidant capacity in vitro (1,1-diphenyl-2-picrylhydrazil (DPPH), reducing power and Fe2+ chelating activity assays) and the toxicity (Artemia salina lethality bioassay) of the extracts were investigated. The phytochemical analyses highlighted quite different phenolic and volatile profiles in the two species. Concerning the antioxidant properties, M. tricuspidata extract resulted the most active both in the DPPH and in the reducing power tests (IC50 = 1.20 ± 0.01 mg/mL and ASE/mL = 11.25 ± 0.97), whereas M. sinuata extract showed better secondary antioxidant properties (IC50 = 1.32±0.02mg/mL). The extracts resulted non-toxic in the Artemia salina lethality bioassay. Our findings indicate that both M. tricuspidata and M. sinuata represent new safe sources of bioactive compounds with antioxidant potential.

Brassica villosa subsp. drepanensis (Caruel) Raimondo & Mazzola, belonging to the Brassica oleracea complex, is a wild edible plant endemic to western Sicily and a relative of modern cultivated Brassica crops. In this study, the antioxidant properties, anti-inflammatory activities, enzymatic inhibition, and cytotoxicity in cancer cells of B. villosa subsp. drepanensis leaf ethanolic extract were analysed for the first time. In addition, its chemical profile was investigated partitioning the total 70% ethanol extract among ethyl acetate, n-butanol, and water to obtain three residues that were subjected to chromatographic separation. Two flavonol glycosides, a phenol glucoside, two amino acids, and purine/pyrimidine bases were obtained. The presence of the glucosinolate glucoiberin was detected in the water extract by UHPLC-MS analysis. The total polyphenol and flavonoid content of the 70% ethanol extract showed good antioxidant capacities and anti-inflammatory properties by reducing nitric oxide release and reactive oxygen species levels and increasing glutathione in lipopolysaccharide-stimulated RAW 264.7 cells. The extract inhibited the enzymatic activity of α-amylase, α-glucosidase, and, significantly, of lipase. The MTT assay showed that the extract did not affect the viability of normal HFF-1 and RAW 264.7 cells. Among the cancer cell lines tested, an antiproliferative action was only observed in CaCo-2. The cytotoxicity of the extract was further confirmed by LDH release assay and by the destabilization of the oxidative balance. Results confirmed the antioxidant properties of the crude extract responsible for the anti-inflammatory effect on healthy cells and cytotoxicity in cancer cells.

In this study, Mentha pulegium leaves and flowers harvested in three different Sicilian areas were investigated from a micromorphological, phytochemical and biological point of view. Light and scanning electron microscopy showed the presence of spherocrystalline masses of diosmin both in the leaf epidermal cells and in thin flower petals. Two different chemotypes were identified (I, kaempferide/rosmarinic acid; II, jaceidin isomer A). Phytochemical screening identified plant from collection site II as the richest in total phenolics (16.74 g GAE/100 g DE) and that from collection site I as the richest in flavonoids (46.56 g RE/100 g DE). Seventy-seven metabolites were identified both in flower and leaf extracts. Plant from site II showed the best antioxidant (0.90–83.72 µg/mL) and anti-inflammatory (27.44–196.31 µg/mL) activity expressed as half-maximal inhibitory concentration (IC50) evaluated by DPPH, TEAC, FRAP, ORAC, BSA denaturation and protease inhibition assays. These data were also corroborated by in vitro cell-based assays on lymphocytes and erythrocytes. Moreover, plant of site II showed the best antiangiogenic properties (IC50 33.43–33.60 µg/mL) in vivo on a chick chorioallantoic membrane. In conclusion, pedoclimatic conditions influence the chemotype and the biological activity of M. pulegium, with chemotype I showing the most promising biological properties.

Alnus glutinosa (namely black alder or European alder) is a tree of the Betulaceae family widely spread through Europe, Southeastern Asia, the Caucasus mountains, and Western Siberia. Its bark is traditionally used for medicinal purposes as an astringent, cathartic, febrifuge, emetic, hemostatic, and tonic, suggesting that it may contain bioactive compounds useful to counteract inflammation. The aim of this study was to investigate the phytochemical profile of A. glutinosa stem bark extract (AGE) by LC-DAD-ESI-MS/MS analysis and to validate some biological activities such as antioxidant, anti-inflammatory and anti-angiogenic properties by in vitro and in vivo models (chick chorioallantoic membranes and zebrafish embryos), that can justify its use against inflammatory-based diseases. The AGE showed a high total phenols content expressed as gallic acid equivalents (0.71 g GAE/g of AGE). Diarylheptanoids have been identified as the predominant compounds (0.65 g/g of AGE) with oregonin, which alone constitutes 74.67% of the AGE. The AGE showed a strong and concentration-dependent antioxidant (IC50 0.15–12.21 µg/mL) and anti-inflammatory (IC50 5.47–12.97 µg/mL) activity. Furthermore, it showed promising anti-angiogenic activity, inhibiting both the vessel growth (IC50 23.39 µg/egg) and the release of an endogenous phosphatase alkaline enzyme (IC50 44.24 µg/embryo). In conclusion, AGE is a promising source of antioxidant, anti-inflammatory and angio-modulator compounds.

Citrus lumia Risso Essential oil Antioxidant properties Anti-cholinesterase activity Cytotoxicity Neuroactive effects 1. Introduction Plants that produce essential oils (EOs) represent a large part of natural flora and an important resource in various fields such as pharmaceutical, food and cosmetic industries, due to their flavor, fra- grance and biological activity (Swamy et al., 2016). EOs play a pivotal role in the growth and colonization of plants, giving color and scent to reproductive organs, attracting pollinators, favoring seed dispersion (Sharifi-Rad et al., 2017), and defending the plant against abiotic (light, temperature, etc.) and biotics (herbivores, harmful insects and pa- thogen microbes) stressors (Sharifi-Rad et al., 2017). More than 250 types of essential oils are commercialized annually on the international market, some of which are used since ancient times in popular medi- cines (Jarić et al., 2015; Mahboubi, 2015), in aromatherapy, and as a therapeutic aid in the treatment of various pathologies including car- diovascular and neurological diseases, as well as diabetes and cancer (Swamy et al., 2016; Lillehei and Halcon, 2014; Solórzano-Santos and ABSTRACT The use of essential oils (EOs) is known since long time in traditional medicine and aromatherapy for the management of various oxidative stress-related disorders and has been further increased recently for their neuroprotective and anti-aging potentials as well as for reducing anxiety and stress. The purpose of this work was to evaluate, for the first time, the chemical composition of Citrus lumia Risso EO and its antioxidant, anti-cholinesterase, and neuroactive properties by cell-free and cell-based assays. The EO has shown strong antioxidant and free radical scavenging properties, particularly in hydrogen atom transfer based assays (β-carotene bleaching and ORAC, IC50 22 μg/mL and 46 μg/mL, respectively), that can be attributed to the high content of monoterpenes, especially D-Limonene (48.905%), and Linalool (18.245%). Furthermore, the EO has shown an interesting anti-acetylcholinesterase activity (IC50 258.25 μg/mL). Data from MTT analysis indicate that the cytotoxicity of EO, evaluated on L929 mouse fibroblasts, is very low, with an IC50 higher than 500 μg/mL at 48 h. Rat neuronal networks subjected to EO showed a concentration-dependent in- hibition of spontaneous electrical activity. Results indicate that C. lumia EO could be an important source of natural antioxidants suggesting an im- portant preventive role in the onset of oxidative stress-related pathologies.

This study explores the factors that influence millennials’ intentions and behavior regarding reduced plastic consumption. An extended theory of planned behavior was established as a conceptual model that explicitly analyzed both the role of past and stated behaviors. The stated behavior was measured using a projective technique. The data obtained from a survey of 741 Italian respondents were analyzed through multiple correspondence analysis and partial least squares structural equation modeling. The results of the projective technique characterized “plastic-free” behavior as a unidimensional construct. Structural equation modeling showed that attitudes, subjective norms, and perceived behavioral control influence the intention of millennial consumers to reduce the use of plastic drinking bottles. The perceived behavioral control is the strongest predictor of intention (ß = 0.304; p < 0.001), followed by social norms (ß = 0.271; p < 0.001) and attitudes (ß = 0.130; p < 0.001). The past behavior construct positively and significantly affects attitude (ß = 0.165; p < 0.001), intention (ß = 0.231; p < 0.001), and stated behavior (ß = 0.073; p < 0.05) constructs. The latter is also positively predicted by intention (ß = 0.151; p < 0.001). Based on the results, actions and incentives for reducing plastic consumption were provided.

As part of a project aimed at investigating the Matthiola taxa endemic to Sicily (Italy), this study focused on Matthiola incana, an edible species used in the traditional medicine of various countries. Herein, the characterization of phenolic and volatile compounds, the antioxidant capacity in vitro (1,1-diphenyl-2-picrylhydrazil (DPPH), reducing power and Fe2+ chelating activity assays) and the toxicity test (Artemia salina lethality bioassay) of the hydroalcoholic extracts from the aerial parts of M. incana subsp. rupestris from Mt. Pellegrino (Palermo) and Mt. Erice (Trapani), and of M. incana subsp. pulchella are reported. The results are compared with those previously shown for M. incana subsp. incana, to achieve a comprehensive overview of the three subspecies. The HPLC-PDA/ESI-MS and SPME-GC/MS analyses led to the identification of 13 phenolics and 54 volatile compounds. Differences in the qualitative-quantitative profile of these phytochemicals have been highlighted between the M. incana subspecies. The antioxidant tests showed different activity for the extracts, which were found to possess better chelating properties. At last, none of the tested extracts displayed toxicity against brine shrimp larvae. These findings enrich the knowledge on the Matthiola taxa growing wild in Sicily, both from the strictly systematic point of view and for the possible applications as sources bioactive compounds that can be used in the nutraceutical field.

In a project designed to investigate the specific and infraspecific taxa of Matthiola endemic to Sicily (Italy) as new potential sources of bioactive compounds in this work, the infraspecific taxa of Matthiola fruticulosa were studied, namely, subsp. fruticulosa and subsp. coronopifolia. HPLC–PDA/ESI–MS and SPME–GC/MS analyses of hydroalcoholic extracts obtained from the aerial parts of the two subspecies led to the detection of 51 phenolics and 61 volatile components, highlighting a quite different qualitative–quantitative profile. The antioxidant properties of the extracts were explored through in vitro methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power and Fe2+ chelating activity assays. The results of the antioxidant tests showed that the extracts possess a different antioxidant ability: particularly, the extract of M. fruticulosa subsp. fruticulosa exhibited higher radical scavenging activity than that of subsp. coronopifolia (IC50 = 1.25 ± 0.02 mg/mL and 2.86 ± 0.05 mg/mL), which in turn displayed better chelating properties (IC50 = 1.49 ± 0.01 mg/mL and 0.63 ± 0.01 mg/mL). Lastly, Artemia salina lethality bioassay was performed for toxicity assessment. The results of the bioassay showed lack of toxicity against brine shrimp larvae for both extracts. The data presented indicate the infraspecific taxa of M. fruticulosa as new and safe sources of antioxidant compounds.

This study explores the factors influencing consumer intention and behaviors regarding consumption of the perceived inedible parts of fruits and vegetables in Ireland and Italy. A conceptual model based on the theory of planned behavior is developed. In addition to the classical relationships of this theory, the constructs of perceived edibility, perceived health benefits, and perceived sustainability benefits are added to the model. An online survey is conducted in both countries, resulting in an overall sample size of 659 participants. Data is statistically analyzed by implementing the partial least squares structural equation modelling. Certain differences exist between Irish and Italian respondents. A significant difference is noted within the social norms construct, whereby Italy scored 9% higher than Ireland. Conversely, Irish people are characterized by higher values for perceived edibility (8%), perceived behavioral control (8%), health (2%), and sustainability benefits (3%). The results indicate that attitude, social norms, and perceived behavioral control affect the intention to consume the perceived inedible parts of fruits and vegetables. Of all these constructs, attitude has the strongest impact on intention, followed by perceived behavioral control and social norms. The results also show that perceived edibility, perceived sustainability, and health benefits have a positive and significant impact on attitudes toward the perceived inedible parts of fruits and vegetables. Practical and policy implications are proposed based on the findings.

Objective We aimed to investigate whether the stratification of outpatients with Coronavirus disease 2019 (COVID-19) pneumonia by body-mass index (BMI) can help to predict hospitalization and other severe outcomes. Patients and Methods We prospectively collected consecutive cases of community-managed COVID-19 pneumonia from March 1st to April 20th 2020 in the province of Bergamo, and evaluated the association of overweight (25kg/m2≤BMI.05). Overall, 105 (31.07%) patients were hospitalized, and time-to-hospitalization was significantly shorter for obese versus over- or normal-weight patients (p

This study aims at estimating the wheat yield response to future challenges (land degradation and climate change) through a statistical emulator. Data from Global Agro-ecological Zones on the overall wheat yields of 254 provinces for nine main wheat-producing countries and corresponding climate and land suitability information were collected to estimate the statistical relationship between regressors and wheat yields. Once the statistical emulator was calibrated, three scenarios were developed to predict, separately and jointly, the impact of climate change and land degradation on wheat yields and productions in 2050 using the global climate model ECHAM4 information under the B2 scenario with the CO2 fertilization effect. Results showed that under temperature and precipitation projections wheat yield would increase on average by 8% (first scenario); however, the impact of future land suitability (second scenario) on wheat yields would be negative, lowering the average yield (−5%), and overall wheat production (−14%). Combining both effects in the third scenario, future wheat yields would increase by 4.6%, while overall production will decrease by 5.5% since the harvest area will be reduced by 9.7%. Thus, land degradation more than climate change is likely to pose significant challenges for overall wheat production in the future.

The aim of the study was to investigate the micromorphology of Mentha pulegium leaves and flowers harvested in three different Sicilian (Italy) areas with peculiar pedo-climatic conditions, and to characterize the phytochemical profile, the phytotoxic activity, and the eco-compatibility of their essential oils (EOs) for potential use as safe bioherbicides. Light microscopy (LM) and scanning electron microscopy (SEM) highlighted that M. pulegium indumentum consists of non-glandular and glandular trichomes of different types. Peltate trichomes of plants from the different sites showed few significant differences in dimension and abundance, but they were characterized by a surprisingly high number of secretory cells both in leaves and flowers. Phytochemical analyses showed that oxygenated monoterpenes were the most abundant class in all the EOs investigated (92.2–97.7%), but two different chemotypes, pulegone/isomenthone and piperitone/isomenthone, were found. The complex of morphological and phytochemical data indicates that soil salinity strongly affects the expression of the toxic metabolite pulegone, rather than the EO yield. Phytotoxicity tests showed a moderate activity of EOs against the selected species as confirmed by α-amylase assay. Moreover, the low toxicity on brine shrimp provided a rationale for the possible use of investigated EOs as eco-friendly herbicides.

The first attempts to draw up plant biodiversity maps in Italy are identified, some of which represent re-workings of data collected for chorological atlases of geographic areas of different extents. These were based particularly on the UTM grid, introduced and codified in Italy by Pignatti [6]. The limited examples made in Italy concern regional, provincial or district areas, some also in protected natural areas. As examples considered original, the maps developed at the Phytotaxonomy and Phytogeography Laboratory of the University of Palermo are presented. These involve the province of Palermo, the Madonie Natural Park in Palermo province, and the eastern Sicani mountains. The map of this last differs from the first two only in the type of reticule adopted for the smaller extent of the base quadrant. The three maps presented constitute complements of more complex maps that focus on the plant landscape of the mapped areas. In these, plant diversity attributed to the individual quadrants of the grid is represented by quantitative data for the specific and infra-specific taxa surveyed, expressed in different color classes. The quantitative incidence of endemic taxa is shown on each map with different symbols (red circles of different diameters), also expressed here by diametric rather than color classes. For the Madonie Natural Park, plant diversity has been mapped with the same quantitative/color criteria, also showing the phytocoenotic richness of this important plant area.

In this paper we discuss current methodologies for measuring biodiversity, analyse pros and cons at different scales, and finally propose distribution mapping as the most suitable method for measuring floristic biodiversity on small Mediterranean islands. These territories often host endangered flora and habitats, and have peculiarities that distinguish them from the mainland and the larger islands.

After a brief review of the recent acquisition in some native genera of family Asteraceae from southern Italy and Sicily, a new endemic species of Anthemis (sect. Hiorthia) is described from Sicily and named Anthemis parlatoreana. The locus classicus of the new taxon, falling in the NW Tyrrhenian coast of the island (Castellammare del Golfo, Trapani), coincides with that of Ptilostemon greuteri, another endemic very rare species of the Sicilian flora. Data on the morphology, distribution, ecology and conservation status of the new species are provided. The taxonomic relationships with the other Sicilian taxa of the same section, in particular A. cupaniana, are also analyzed. The simultaneous presence in the same site of other endemic plants enriches the area of the limestone reliefs of Castellammare del Golfo and all of north-western Sicily with biogeographic significance and biogenetic importance.

The relationship of two geographically and ecologically separated populations of Turraea socotrana Styles and F. White (Meliaceae), a vulnerable and scattered shrub from the island of Socotra (Yemen), was explored using preliminary molecular data. The nuclear ribosomal ITS region was used to screen the level of genetic divergence of T. socotrana from ecologically distant localities in comparison to a few other species of Turraea and closely related genera. The individuals from the inland (Diksam) differed from the western coastal ones (Ma’alah) by vegetative characters and by the rbcL gene. This may be due to the fact that the population from Ma’alah grows on lithosoil slopes directly exposed to moist winds coming from the sea, whereas the population of Diksam occurs on arid cliffs overlooking the inland of the island. The ITS sequences grouped the studied populations from Socotra together and separated them from other species of Turraea that are morphologically similar andoccurring in East Africa. Considering the differences recorded in the rbcL gene, we suggest that the populations of T. socotrana investigated here on only a few specimens from two different areas on the island, deserve more research. Our preliminary study potentially indicates that there may be independent evolutionary significant units in this species, however, more research including a wider sampling and comparison to other taxa is needed.

According to projects and practices that the Italian botanists and ecologists are carrying out for bringing “more nature in the city”, new insights for a factual integration between ecological perspectives and more consolidated aesthetic and agronomic approaches to the sustainable planning and management of urban green areas are provided.

The historical cultivation of common bean (Phaseolus vulgaris L.) has resulted in the development of local populations/cultivars in restricted Italian rural areas. Many common bean landraces, still cultivated in small mountain areas from Sicily, have become outdated and endangered due to the commercial varieties spreading. These accessions are poorly known but often represent a genetic heritage to be preserved and enhanced. The ex situ conservation of fifty-seven Sicilian common bean landraces was carried out at the &ldquo, Living Plants Germplasm Bank&rdquo, at Ucria (Messina, Italy), founded by the Nebrodi Regional Park, together with the &ldquo, Sicilian Plant Germplasm Repository&rdquo, of University of Palermo (SPGR/PA). To assess the germplasm genetic diversity, nineteen morphological traits and eight Simple Sequence Repeats (SSRs) were used. Genetic distances among landraces were calculated to construct a clustering tree by using unweighted pair group method arithmetic (UPGMA). Seed germplasm diversity of Sicilian common bean varied from 80.7% to 93.3%, based on six seed descriptors and six leaf, flower, and pod descriptors, respectively, while cluster genetic analysis depicted a clear separation among all the 57 landraces. Principal coordinates (PCoA) and STRUCTURE analyses showed a prevalent rate of admixture between Mesoamerican and Andean gene pools in Sicilian common bean collection, confirming its heterogeneity. The observed high level of diversity evidenced the needs to adopt accurate criterion to plan a definitive ex situ germplasm collection to share agrobiodiversity with local farmers and to avoid any further loss of genetic resources in rural and protected areas.

In Italia, secondo la recentissima sintesi di Bartolucci et al., la flora vascolare nativa sarebbe rappresentata da 1092 generi di piante vascolari. Fra di essi ricorre Sorbus L. (Rosaceae) a cui vengono attribuiti 11 taxa specifici e sottospecifici (ivi compresi alcuni taxa ibridogeni) e un dodicesimo taxon da confermare nel territorio italiano. In questo contributo si vuole porre attenzione proprio alla recentissima checklist europea di Sorbus s.l. basata su acquisizioni di carattere filogenetico sul gruppo e, più in generale, sulle Pyrinae. In particolare, si fa riferimento allo studio di Sennikov, Kurtto in cui Sorbus L. viene praticamente smembrato in numerosi altri generi, mentre i taxa totalmente o in parte ibridogeni vengono riferiti ad altri di nuova istituzione. Quest’ultimo trattamento, se accettato, avrà riflessi sulla diversità intergenerica della flora europea e quindi anche di quella italiana che si arricchirebbe così di ben altri 5 generi (Aira, Cormus, Chamaemespilus, Torminalis e Hedlundia). A seguito del nuovo arrangiamento, il taxon generico linneano finisce con il comprendere una sola unità specifica (Sorbus aucuparia). Dunque, il quadro generale della flora europea viene a subire una considerevole variazione introducendo ben 9 generi aggiuntivi fra cui Aira, Cormus, Chamaemespilus e Torminalis nonché i nuovi: Hedlundia, Carpatiosorbus, ×Borkhausenia, ×Ma jovskya e ×Normeyera. Per la flora italiana, le variazioni quantitative sono più a livello generico piuttosto che specifico: 6 generi al posto di uno; 11 taxa specifici e sottospecifici distribuiti in 6 generi piuttosto che in uno solo. Si riporta il prospetto dei generi e dei taxa specifici e sottospecifici correlati a Sorbus s. lato e riferiti al territorio italiano in base al suddetto recente studio.

Based on recent literature, in the Primrose family the genus Lysimachia L. absorbed Anagallis L. (1). As a result, new name combinations have been introduced for this genus, thus involving the increase of specific and intraspecific taxa (for Italian flora see 2 and 3). A further consequence, focused in this contribution, concerns the binomial combination Lysimachia arvensis (L.) U.Manns & Anderb., which has different synonyms and includes several specific and intraspecific taxa. As far as the taxonomic history of the annual taxa of Anagallis is concerned, different authors preferred to include some of them in A. arvensis and in A. foemina at the level of variety (4) or subspecies (2), while others recognized their specific rank (5, 6, 7). Among these taxa, A. parviflora Hoffmanns. & Link is frequently reported, even included among the synonyms of A. arvensis (5) and then considered as a distinct species (7). The already complex status is further complicated by the occurrence of hybrids, as reported by Pignatti (6). The cited case would regard A. foemina and A. parviflora, but other taxa are also mentioned in literature [cf. Anagallis × intermedia Giraudias = Lysimachia × intermedia (Giraudias) B.Bock [(Lysimachia arvensis (L.) U.Manns & Anderb. × Lysimachia foemina (Mill.) U. Manns & Anderb.]. The analysis of the variability observed in a Sicilian population referable to this group (Fig. 1) revealed a quite critical case, introducing additional variables in the different interpretations of varieties, forms and hybrids of this group of complex species. Plant samples collected in the territory of Palermo particularly showed new colours of the corolla and different dimensions of petals. This recurrence of form and colour observed in the same station for two years, with the presence of congeneric taxa, allows to hypothesize a temporary recurrence of the mentioned features; the authors were induced to interpret this population at variety level or consider it the result of recurringhypothesize a temporary recurrence of the mentioned features; the authors were induced to interpret this population at variety level or consider it the result of recurring hybridization processes. Field researches were extended on this basis and a second population was found in the territory of Misilmeri, near Palermo. Both populations coexist with the best known and widespread A. arvensis (incl. A. phoenicea Scop.) and A. foemina. The possible hybrid origin of the new type – elsewhere reported by amateurs not just in Italy – led to start genetic investigations which are still in progress.

Different species and varieties of bean, spread in Sicily, are representative of local agricultural practices, as result of a careful exploration. Many landraces have become obsolete due to the spread of commercial varieties, but are still cultivated in small areas of Nebrodi Mountains (ME-Italy) and are endangered. The Sicilian bean landraces are often poorly known but represent a genetic heritage to be preserve and to enhance. The ex situ conservation of Sicilian bean landraces was carried out in “Living Plants Germplasm Bank” of Ucria (ME-Italy), founded by the Nebrodi Regional Park, and in “Sicilian Plant Germplasm Repository” of STEBICEF Department - University of Palermo. Within ex situ germplasm conservation the “core collection” represents the maximum genetic diversity without redundancy stored in a gene bank, providing an overall view of the properties to be found in a whole collection and increasing the efficiency of characterization and utilization of collection. The aim of this study was to develop a core collection of representative Sicilian bean genotypes, by morphological characterization. Flowers, pods and seeds of Phaseolus vulgaris L., Phaseolus coccineus L. and Vigna unguiculata L. cultivars were collected from plants grown under same pedo-climatic condition at “Living Plants Germplasm Bank” of Ucria and used for morphological characterization according to the IPGRI descriptors. The analysis of the seeds has allowed to identify 35 morphotypes within the collection, indicated by a number code (5 descriptors). The 68,4% of landraces showed seed coat with pattern and the 46% exhibited a cuboid shape. The main representative seed-morphotype was the 27513 code (6 landraces) characterized by striped pattern coat, seed bicolor (purple and pink) with ligther prevalent and cuboid shape. In general, multiallelic control and environmental factors contribute to color expression variation in flower, pod and seed coat of bean. The results, observed in field collection, shows that the purple flowers are often linked to darker seed development, while white or liliac flowers are linked to lighter seeds. In summary, the results showed a redundancy of some bean accessions supporting the hypothesis that same cultivar is named in different way by the local farmers. Further genetic investigation will be needed to confirm the suggesting cases of synonymies between Sicilian accessions.

The genus Alnus (Betulaceae) comprises many species with a long history in traditional medicines. The crude extracts and isolated compounds from Alnus species exhibit a wide spectrum of in vitro and in vivo pharmacological activities (1). Phytochemical investigations revealed the presence of diarylheptanoids, a class of natural products typically found in Alnus genus with two aryl groups joined by a heptane chain in the main skeleton that have drawn attention due to their multiple biological properties and their therapeutic potential (2). A previous study reported that oregonin and other structurally analogous diarylheptanoids isolated from the bark of A. hirsuta showed inhibitory effects on melanogenesis in B16 melanoma cells.(3). Nowadays the discovery of new whitening agents from natural sources is increasing, due to the weak effectiveness and unwanted side effects of currently available compounds. In this context, the aim of this study was to evaluate the skin whitening capabilities of crude extracts (80% aqueous MeOH) obtained from the fresh bark of Alnus glutinosa (L.) Gaertn. and Alnus cordata (Loisel) Duby, an endemic species in the Mediterranean areas (4). As tyrosinase is the rate-limiting enzyme in melanin biosynthesis, the inhibitory effects of A. glutinosa and A. cordata extracts (AGE and ACE, respectively) on mushroom tyrosinase activity were preliminary evaluated. In addition, the anti-melanogenic ability of AGE and ACE was further investigated on the pigmentation of early stage zebrafish at 72 hours post fertilization (hpf) to find new skin whitening agents without cytotoxic concerns. Results of the enzymatic assay showed that ACE was capable to inhibit dose dependently L-DOPA oxidation catalyzed by tyrosinase (IC50 = 77.44 ± 0.54 μg/mL) as compared to the reference inhibitor kojic acid (2.24 ± 0.18 μg/mL). Unlike, AGE exhibited a lower anti-tyrosinase activity (100 μg/mL reached 28% of inhibition while higher doses showed pro-oxidative effects). Moreover, the zebrafish in vivo assay revealed that ACE (50 μg/mL) has equivalent inhibitory effects on the pigmentation (76.57%) to that of phenylthiourea (PTU, 30 μg/mL), used as the reference inhibitor (77.80%), as compared to control, while they did not affect the embryos development and survival. Conversely, the depigmenting effects of AGE were about 10 fold less than ACE (45.28% at 500 μg/mL). A mild anti-melanogenic activity was also evidenced for the diarylheptanoid oregonin (10% of inhibition at 20 μg/mL). A preliminary phytochemical screening evidenced that ACE and AGE have a high phenolic content (399.27 ± 14.30 and 534.17 ± 20.60 mg GAE/g of extract, respectively). However, despite AGE showed the highest phenolic content, the quali-quantitative RP-HPLC-DAD analysis highlighted as it is predominantly composed by oregonin (418.45 μg/mg of AGE vs 1.23 μg/mg of ACE) that exhibited a mild anti-melanogenic activity both in vitro and in vivo assays. Further phytochemical investigations are still in progress to identify the bioactive compounds of ACE as to be considered a potential candidate for the treatment of skin disorders due to its bleaching properties and favorable safety profiles.

The current study investigates the willingness of Millennial consumers towards several corporate social responsibility initiatives carried out by food companies. More specifically, it explores four cause related marketing campaigns implemented by food companies to spread corporate social responsibility. The analysis was carried out in Italy by administering a structured questionnaire to 308 consumers. The willingness of participants to switch a chocolate snack of their favourite brand to another with similar characteristics but produced by a company supporting different social and environmental causes was assessed in four different scenarios. The study uncovered the effects of both loyalty towards the brand and trust in cause related marketing on consumers&rsquo, willingness to support different corporate social responsibility initiatives. The findings unveiled the willingness of Millennials to support companies&rsquo, social and environmental initiatives. Both trust and loyalty played a key role in affecting consumers&rsquo, willingness to support corporate social responsibility initiatives of food companies. Social and environmental concerns as well as socio-demographics aspects are also significant in supporting cause related marketing campaigns. The study has shed light on the preference of consumers towards corporate social responsibility and cause related marketing. Specifically, it provides marketing insights on the initiatives most preferred by consumers to which companies should address their efforts.

In this study, we investigated the phenolic composition of the crude extract (MeOH 80 %) of Alnus cordata (Loisel.) Duby stem bark (ACE) and its antioxidant and skin whitening properties. RP-LC-DAD analysis showed a high content of hydroxycinnamic acids (47.64 %), flavanones (26.74 %) and diarylheptanoids (17.69 %). Furthermore, ACE exhibited a dose-dependent antioxidant and free-radical scavenging activity, expressed as half-maximal inhibitory concentration (IC50 ): Oxygen radical absorbance capacity (ORAC, IC50 1.78 μg mL-1 )>Trolox equivalent antioxidant capacity (TEAC, IC50 3.47 μg mL-1 )>2,2-Diphenyl-1-picrylhydrazyl (DPPH, IC50 5.83 μg mL-1 )>β-carotene bleaching (IC50 11.58 μg mL-1 )>Ferric reducing antioxidant power (FRAP, IC50 17.28 μg mL-1 ). Moreover, ACE was able to inhibit in vitro tyrosinase activity (IC50 77.44 μg mL-1 ), l-DOPA auto-oxidation (IC50 39.58 μg mL-1 ) and in an in vivo model it exhibited bleaching effects on the pigmentation of zebrafish embryos (72 h post fertilization) without affecting their development and survival. In conclusion, results show that A. cordata stem bark may be considered a potential source of agents for the treatment of skin disorders due to its bleaching properties and favorable safety profiles, associated to a good antioxidant power.

The group of Centaurea (sect. Acrolophus) cineraria (Compositae) in Sicily includes four currently accepted species plus four taxa that have been considered, with some doubt, as subspecies of one of them (C. panormitana). In total, discounting recombinations and replacement names, 11 names (seven specific, three varietal, one of a forma), all based on Sicilian material, were published by past authors for these eight taxa. Two of the names had their holotype indicated upon publication; two have been lectotypified previously; six (C. busambarensis var. obtusiloba, C. cineraria var. soluntina, C. cineraria var. umbrosa, C. todaroi, C. todaroi f. seguenzae and C. ucriae) are lectotypified here; and C. panormitana is neotypified. The whole group is extremely variable and the delimitation of most taxa is still uncertain. Pending further study, including the sampling of wild populations, no taxonomic reassessment is therefore attempted here, with one exception: the merger of C. panormitana subsp. ucriae with C. panormitana subsp. panormitana.

Abies nebrodensis, endemic to the Madonie mountains (central-north Sicily), was originally described by Lojacono Pojero as A. pectinata var. nebrodensis and later raised to the rank of species. The species is considered to be critically endangered and was thought to be extinct until an extant population was rediscovered in 1957. The name is here lectotypified on a specimen preserved at NAP.

From 2012–2016 plants of basil (Ocimum basilicum L.) and parsley (Petroselinum sativum Hoffm.) showing decline symptoms were collected from local markets in Foggia Province (southern Italy) and assessed to determine their main fungal pathogens. These plants showed symptoms including leaf yellowing, necrotic lesions on stems, collar and roots, and in some cases, stunting of entire plants. Mycological analyses revealed fungal isolates mainly belonging to the Plectosphaerella genus. Molecular and morphological studies identified four species of Plectosphaerella: Plectosphaerella cucumerina, P. pauciseptata, P. plurivora, and P. ramiseptata. To understand the pathogenic roles of these fungi, and five other reference Plectosphaerella spp., pathogenicity tests were performed in vitro and in-vivo using, respectively, detached leaves and 30-d-old plants of basil (cv. Napoletano) and parsley (cv. Gigante di Napoli). All the fungal species isolated produced host symptoms, including necrotic spots, parenchymatic patches, hydropic areas, collar and root discolouration on leaves and young plants, with varying severity. The most aggressive species on both plants were P. pauciseptata and P. ramiseptata, while P. alismatis, P. citrulli, P. cucumerina, P. delsorboi, P. melonis, and P. plurivora gave less disease severity on both plants. This is the first report worldwide of P. cucumerina, P. pauciseptata, and P. ramiseptata as pathogens of parsley, and P. pauciseptata, P. plurivora, and P. ramiseptata as pathogens of basil.

In Sicily, the genus Helleborus (Ranuculaceae) is only represented by H. bocconei subsp. siculus (= H. bocconei subsp. intermedius). In some mountain areas of the Island, the rhizomes of this plant, harvested in a particular month of the year (May) and dried, are used in traditional veterinary practice for treating pneumonia in domestic animals, cattle and horses in particular. The same usage – with rhizomes of other Helleborus species or subspecies – is reported from various other areas of Mediterranean Europe. Phytochemical tests have permitted the isolation and characterization of new biologically active molecules. The extracts of rhizomes and aerial parts of the plant were shown antibacterial properties. Some compounds, isolated from the methanolic extract of the rhizomes, were shown to be cytotoxic. In addition, morpho-anatomical studies have revealed the presence of different endophytic and commensal fungi in all organs of the plant, which could be isolated and cultured. One of the isolates has been identified as the endophytic fungus Botrytis byssoidea, which is also widely present in the soil. Further fungal isolates include Chaetomium strumarium, strain RR1, an endophytic ascomycete the identity of which was confirmed by molecular analyses. When cultured, it developed plentifully; the filtered broth from these cultures was used in antibiotic property assays. The tests were positive; the detailed results are forthcoming. They support our initial hypothesis, that the therapeutic effect the hellebore’s rhizomes extract is due to metabolites produced by an endophytic fungus. It remains to be seen whether the plant itself, devoid of the microfungus, produces the same therapeutically effective metabolites that are present in the extracts of plants from the wild or from outdoor cultivation. The study of the Sicilian hellebore, beyond its biological interest, has potential for its relevance for therapeutic applications both in veterinary and human medicine.

An updated inventory of the vascular flora alien to Italy, providing details on the occurrence at regional level, is presented. The checklist includes 1597 species, subspecies, and hybrids, distributed in 725 genera and 152 families; 2 taxa are lycophytes, 11 ferns and fern allies, 33 gymnosperms, and 1551 angiosperms. 157 taxa are archaeophytes and 1440 neophytes. The alien taxa currently established in Italy are 791 (570 naturalized and 221 invasive), while 705 taxa are casual aliens, 4 are not assessed, 7 are of unknown regional distribution, 47 have not been confirmed in recent times, 3 are considered extinct or possibly extinct in the country, and 40 are doubtfully occurring in Italy. This checklist allows to establish an up-to-date number (9792) of taxa constituting the whole (native and alien) Italian flora.

Hieracium sartorianum var. lucanicum, a little known taxon described from Mt Sacro (Parco Nazionale del Cilento e Vallo di Diano, Prov. of Salerno, Campania, Italy), is reexamined. As a result it is reclassified as a subspecies to H. hypochoeroides. A new combination and lectotypification are made and a detailed description is given.

Crop variety mixtures (different varieties of a crop grown together in a single plot) have been successfully deployed in pathogen and pest management for several crops including wheat, common bean and rice. Despite the available evidence, promotion of this approach has remained limited in many countries, including Uganda. The factors that influence farmers' adoption of varietal mixtures for common bean and banana were assessed, as well as the perceptions of farmers on the effects of mixtures on yields, through household surveys and statistical modelling. A three-year yield increase in both common bean and banana varietal mixtures in farmer fields, of 5.2% and 28.6%, respectively, is realized using robust OLS estimates. The study reveals that accessing knowledge on the importance of crop varietal mixtures and the skills relating to the approach are crucial for their adoption. Location of the farm significantly determined the perceived yield change, which calls for more research into mixtures' suitability under particular contexts in respect to compatibility of genotypes, management practices and appropriate acreage for maximum impact. The positive effects of mixtures on yields make it an effective biologically friendly economy strategy. Policies that minimize the adoption barriers could improve the adoption of crop varietal mixtures on a wider scale.

Summary. Young grapevine plants with decline and wood necrosis symptoms were collected from vineyards and nurseries in the Apulia and Molise regions, Italy, from 2013 to 2015. Isolations of fungi were prepared from 45 diseased grapevine plants, and the cultures were identified. Several species commonly associated with Petri disease, Botryosphaeria dieback, and black foot disease were isolated. A detailed study was carried out, and 182 isolates resembling Cylindrocarpon-like asexual forms were identified through morphological characterisation and DNA analysis of internal transcribed spacer regions 1 and 2 of the rRNA gene and the partial β-tubulin gene. Dactylonectria torrensensis and Ilyonectria liriodendri were identified based on morphological features and the partial histone 3 gene, so these fungi can be defined as the causal agents of black foot on grapevine for the first time in Italy. Thelonectria blackeriella is also described as a new species, through morphological characterisation and multigenic analysis using sequence data for five loci (large subunit RNA, internal transcribed spacers, β-tubulin, actin, RNA polymerase II subunit 1). This new species was associated with black foot symptoms according to preliminary pathogenicity tests, with representative isolates of each of the three species. Pathogenicity tests showed that these species can cause black streaking in the wood of 1-year-old grapevine rootstock shoots. The identification of D. torresensis, I. liriodendri and T. blackeriella from young grapevine plants and rooted rootstock highlights the importance of black foot disease in Italy, which has previously been overlooked.

We conducted a GIS spatial analysis with the aim of providing the first quantitative large-scale overview of the distribution patterns of 1536 type localities ( loci classici ) of 1216 Italian endemic vascular plants and their relationship with a set of descriptive variables. Whereas some variables were used to model the presence-absence distribution patterns of the type localities for the whole set of endemics as well as for the subset of narrow endemics, others (e.g., presence inside or outside protected areas and Italian Important Plant Areas) were considered with the purpose of assessing potential assets or risks for conservation. The largest number of type localities was found within the Mediterranean biogeographic region (1134), followed by the Alpine region (306) and Continental region (96). A total of 670 locations are located on islands, whereas 866 are located on the Italian mainland (139 and 124 in the case of narrow endemics, respectively). A large number of type localities are located in mountainous areas and along the coastline, which can be seen as a potential risk for conservation. On the contrary, we detected a positive correlation with the distance from roads, which might be considered to be an asset. Importantly, 1030 type localities fall inside protected areas, whereas 506 localities fall outside protected areas, with 259 of these unprotected localities on islands. We propose considering the results of the analysis of the distribution of type localities of Italian endemics to be a strategic tool for conservation planning and resource management. Application of plant micro-reserves and integration of diverse legislation tools are suggested to strengthen efforts and increase conservation success.

It is reported the presence in Sicily of a new alien species destined to a progressive further spread in the Island. It is Bidens subalternans DC., an annual asteracea of American origin, already known in other Italian regions (Friuli Venezia Giulia, Lombardia, Marche, Lazio and Campania) and in other European countries (Belgium, Corse, Croatia, Cyprus, France, Germany, Great Britain, Montenegro, Republic of Serbia, Slovenia, Spain, Switzerland). The new locality, in Sicily, occurs along the roadside Monreale – Pioppo, south of the city of Palermo. In this station, B. subalternans is found together with B. pilosa subsp. minor and other indigenous and exotic nitrophilous taxa. The population observed near Monreale (Palermo), is quite large and, according to the methods of dispersion, shows in Sicily an invasive tendency in favourable environment. The affinities of B. subalternans with B. bipinnata L. – with which the species was sometimes confused – is also discussed.

The chemical composition of the essential oils isolated from the aerial parts of Anthemis arvensis L. subsp. arvensis, Anthemis cretica subsp. messanensis (Brullo) Giardina & Raimondo and from flowers and leaves of Anthemis cretica subsp. columnae (Ten.) Frezén were determinated by GC–FID and GC–MS analyses. Torreyol (85.4%) was recognised as the main constituent of the Anthemis arvensis subsp. arvensis essential oil, while in the essential oils of Anthemis cretica subsp. messanensis, collected on the rock and cultivated in Hortus Botanicus Panormitanus, (E)-chrysanthenyl acetate (28.8 and 24.2% resp.), 14-hydroxy-α-humulene (8.1 and 5.3% resp.), santolina triene (8 and 5.8% resp.) and α-pinene (6.7 and 5.4% resp.) prevailed. 18-cineole (13.3 and 12.2% resp.), was the main component of both flower and leaf oils of Anthemis cretica subsp. columnae together with δ-cadinene (9.0 and 8.2% resp.) and (E)-caryophyllene (8.3 and 5.6% resp.).

A non-sporulating fungus was isolated from different organs of Helleborus bocconei subsp. intermedius (Ranunculaceae) endemic to southern Italy and Sicily, known for the traditional use of dried roots in the treatment of lung diseases of cattle and horses. Molecular characterization of endophytic fungus based on the internal transcribed spacer (ITS) region of the rRNA gene sequences was done. The DNA sequence of full length ITS region of the studied fungus was a 100% match to that of Chaetomium strumarium strain dH 21642 (GenBank accession number JX280851.1). The morphological characters of colony and mycelium of this microfungus are reported here.

Names of 20 presumed taxa in Draba sect. Aizopsis, all based on material from Italy, are considered. Full synonymies are provided, and types are designated (for 14 names) or indicated. Most of the taxa are currently considered unworthy of recognition, of which 16 belong to D. aspera sensu lato. However, the question of whether the Sicilian populations might be distinct from the peninsular populations is still unsettled.

Nella flora vascolare indigena d’Italia, costituita da circa 7000 taxa specifici e infraspecifici, quelli arborei sono rappresentati da un modesto numero di unità. Tra gimnosperme e angiosperme non si superano 200 specie. Diversificano e arricchiscono la dendroflora italiana specie legnose policormiche o arbustive che, con l’età e in particolari condizioni, possono assurgere a veri e propri alberi. Vi contribuiscono anche espressioni genetiche di classiche specie arboree variamente interpretate a livello tassonomico. La dendroflora qui esaminata si riferisce a 53 generi (7 gimonosperme e 46 angiosperme). I taxa specifici e infraspecifici sono 188 (21 gimnosperme e 167 angiosperme). Non pochi generi presentano una elevata diversità (Pinus, Quercus, Salix, Pyrus, Tamarix). La famiglia Rosaceae è la più ricca in generi con Crataegus, Malus, Mespilus, Pyrus, Prunus e Sorbus. Nella famiglia Fagaceae, espressa dai generi Castanea, Fagus e Quercus, i primi due sono monospecifici mentre Quercus esprime la maggiore diversità tassonomica della dendroflora riferita ad uno stesso genere. La famiglia Salicaceae viene espressa dal solo genere nominale Salix e in questo, dunque, si concentra tutta la sua diversità tassonomica, quantitativamente rilevante pressoché quanto in Quercus. Dal punto di vista corologico, la dendroflora italiana presenta caratteri prevalentemente europei e subordinatamente mediterranei; l’endemismo ha una considerevole incidenza ed è preminentemente rappresentato da unità sottospecifiche; non vi mancano tuttavia casi di buone specie come in Abies, Betula, Malus e Pyrus

Di Gristina, E., G. Gottschlich & F.M. Raimondo (2016). Pilosella hoppeana subsp. sicula Di Grist., Gottschl. & Raimondo (Asteraceae), a new endemic subspecies from Sicily (Italy). Candollea 71: 7–12. In English, English abstract. DOI : http://dx.doi.org/10.15553/c2016v711a2 Pilosella hoppeana subsp. sicula Di Grist., Gottschl. & Raimondo (Asteraceae), a new endemic subspecies from the Madonie and Nebrodi Mountains (Sicily, Italy), is described and illustrated. It is morphologically close to Pilosella hoppeana subsp. macrantha (Ten.) S. Braut. & Greuter, but the two subspecies can be distinguished by the indument on the bracts. Its morphology, distribution, ecology, taxonomic relationships are commented and a key to the Pilosella hoppeana (Schult.) F.W. Schultz & Sch. Bip. aggregate is provided. Received: October 16, 2014; Accepted: September 9, 2015; First published online: March 14, 2016

Hieracium nebrodense Tineo ex Lojac., a little-known endemic of Sicily, has been rediscovered from the type locality after almost two centuries since its first and only collection made in 1830 in the Madonie Mountains (N Sicily). In line with the treatment in ‘Med-Checklist 2’, it is reclassified as a subspecies of H. schmidtii. A new combination is made and a detailed description and illustration are provided.

Aim of the present work is the analysis (through the study of enzyme polymorphism) of Sicilian and African (Tunisian) populations of Ambrosina bassii , a small perennial endemic to the Central-Western Mediterranean basin, in order to verify if the complex geological history of this part of the Mediterranean area left its mark in the present-day genetic structure of this taxon. Starch gel allozyme electrophoresis of seven putative loci of A. bassii was employed to estimate genetic diversity, genetic structure and gene flow. Populations from Sicily, Tunisia and Sardinia (as outgroup) were sampled. Results show that Sicily populations have 4 private alleles, Sardinia 3, Tunisia just one. One allele is private to both Sardinia and Tunisia, another one to Sardinia and Sicily. Even if there are no alleles private to Sicily and Tunisia, “cluster analysis” (based on Nei's genetic distances), “non-metric multidimensional scaling” (computed on the basis of a matrix with F ST values between populations) and Bayesian analysis point out a clear isolation of Sardinian populations, and a greater similarity between Sicilian and Tunisian populations compared to Sardinian ones. The strong genetic affinity between populations from Sicily and Tunisia, considering the very low dispersal ability of the species, gives evidence of a recent continuity between the populations as well as between the two areas. Considering also the estimates of divergence times, a post-Messinian terrestrial connection between the two landmasses can be hypothesized.

Limonium cedrorum Domina & Ramondo, a new species belonging to the L. palmare aggr., is described and illustrated from the inland near Becharre (Lebanon). Its relationships with morphologically close taxa are discussed. Limonium cedrorum differs from L. sieberi and L. postii mainly by looser inflorescences and larger flowers. It differs from L. galilaeum by longer outer bract and longer scale below the first inflorescence branch, few sterile branches, thicker base of the inflorescence and denser spikes, and from L. graecum by the shape of the infl orescence, longer basal internodes and the shape of the calyx.